정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

제2형 당뇨병 외래 환자의 비만도에 따른 미세혈관합병증

이성규,조남한,김현만,이관우,정윤석,채봉남,김윤정,이미덕,노혜림,홍은경,조현경 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.1

Background: Korean type 2 diabetic patients who are frequently non-obese, may be genetically different from Western type 2 diabetics who are frequently obese. Therefore, the diabetic complications of type 2 diabetes mellitus in Korea may be also different from those of Western countries. Until now, most studies reported in Korea did not analyse the microvascular complications of type 2 diabetes mellitus according to obesity, and also the criteria in the diagnosis of microvascular complications were different in each study. We investigated the microvascular complications and its relationship with obesity, in type 2 diabetic patients visiting an outpatient clinic. Methods: The study subjects were type 2 diabetic patients visiting an outpatient clinic of Ajou University Hospital. We selected patients participating in a 75 g oral glucose tolerance test, retrospectively. Type 2 diabetes was diagnosed according to the WHO/NDDG classification of diabetes. Biochemical studies including lipid profile, plasma insulin and C-peptide levels were done. Anthropometric measurements were performed. Based on BMI (㎏/㎡), the patients were divided into the following groups: the lean group, when the BMI was less than 20 ㎏/㎡ the ideal body weight (IBW) group, if the BMI was between 20 ㎏/㎡ and 25 ㎏/㎡ in women and 20 ㎏/㎡ and 27 ㎏/㎡ in men; and the obese group, when the BMI was $gt;25 ㎏/㎡ in women and $gt;27 ㎏/㎡ in men. Results: 1. Neuropathy (45.2%) was the most frequent among the microvascular complications, and the frequency of retinopathy was 15.1%, and that of nephropathy was 4.9%. Within 5 years of diabetes duration, the frequency of neuropathy, retinopathy, and nephropathy was 43.2%, 11.8%, and 2.9%, respectively. 2. Glycosylated hemoglobin (HbA_(1c)) and fasting blood glucose levels were not different among the three groups. Beta cell function{Δ (insulin 30min - insulin Omin)/Δ(glucose 30min - glucose 0min)} was the highest in the obese group. However, beta cell function(ΔI/ΔG) divided by the basal insulin level, considered insulin resistance, was not different among the three groups. 3. Within 5 years of diabetes duration, retinopathy tended to be the most frequent in the lean group, whereas neuropathy tended to be the most frequent in the obese group, and body mass index influenced the retinopathy and neuropathy, statistically significantly. Conclusion: Diabetic neuropathy was the most frequent among microvascular complications of type 2 diabetes mellitus in our study subjects. At the time of presentation within 5 years of diabetes duration, the lean group of type 2 diabetics had a tendency of the more frequent retinopathy, the obese group had a tendency of the more frequent neuropathy. These results suggest that type 2 diabetes mellitus in Korea is also not a single disease entity, as in Western countries and is a heterogenous group of disorders with a diversity of microvascular complications. However, the more studies about this will be required.

전자부품 제조업 근로자들의 시력변화에 대한 연구

이용만,안규동,이성수,함정오,김화성,리갑수,이병국 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

근접작업이 시력약화의 원인이 될 수 있는가를 알아보기 위하여 전자부품을 제조하는 2개 사업장에서 1991년에서 1993년까지 입사한 근로자들 중에서 근접작업으로 인정되는 마이크로칩을 조립하는 근접작업자 306명을 조사군으로, 대조군은 세탁기 타이머 및 일반 기계기구를 조립하는 작업자 154명, 계 460명을 선정하였다. 이들 대상자는 1991년 입사자는 1993, 1994, 1995년 시력을 조사하였으며, 1992년 입사자는 1993, 1994, 1995년 3년동안 조사를 하였으며, 1993년 입사자는 1994, 1995년 2년간 시력을 조사하였다. 대상자 중에서 입사시 안경을 착용한 근로자는 82명(17.8%)이었다. 따라서 남자 근로자와 안경착용자를 제외한 여성 근로자들 335명만을 추적 조사하여 다음과 같은 결론을 얻었다. 조사군에서 입사시 시력 1.0 이상이었던 근로자의 분포는 취업 1년 후 약 25-35% 정도 감소하였으며, 2년후에는 약 40-60% 정도의 감소를 나타내었고, 3년 후 약 50-65% 정도로 감소하였고 4년후에는 약 65% 이상 감소하였다. 그러나 대조군에서는 1.0 이상의 시력을 보였던 근로자들의 비율이 취업 1년 또는 2년 후 15-30%의 감소를 보이고 있으나 그 이후에는 큰 변동이 없었다. 이들의 시력변화를 근무년수에 따라 알아보기 위하여 조사된 시력을 순위변수로 하여 Kruskall-Wallis 검정을 시행한 결과 조사군, 대조군 모두에서 근속년수에 따른 순위의 합의 평균이 년차적으로 유의하게 감소하였으나, 입사시부터 근속년수에 따른 조사군과 대조군과의 순위합의 평균에 의한 시력차 비교에서 입사시 조사군의 순위합의 평균이 유의하게 높았던 것이 근무기간에 따라 대조군의 평균이 높아지면서 통계적 유의성이 없고 대체적으로 1년 이후부터 변화가 적은 것으로 나타나 근접작업으로 인한 시력약화를 추정할 수 있었다. This study was carried out to investigate that the close distance works were associated with decreased visual acuity. 306 workers were subjects who had been recruited from 1991 to 1993 in two electronic parts manufacturing factories, and 154 workers were controls who had been employed from 1991 to 1993 in launder machine timer and general machine assembly factories. Subjects who had been employed in 1991 and 1992 were follow up from 1993 to 1995, and subjects who had been employed in 1993 were from 1994 to 1995. The male workers as well as glasses wearing workers were excluded. Only 335 female workers were followed up to study the change of visual acuity. The results were as follows; The rate of workers with visual acuity over 1.0 at recruit year in subjects group was decreased about 25-35% after 1 year, 40-60% after 2 years, 50-65% after 3 years, and 65% after 4 years. While, the rate of workers with visual acuity over 1.0 at recruit year in control group was decreased about 15-30% after 1 or 2 years, and there was no more additional visual acuity reduction after then. Kruskall-Wallis test was performed to investigate the change of visual acuity in subject and control group by work duration. The means scores of ranks in both group were significantly decreased by work duration. The mean scores of ranks in subject group at recruit year were significantly higher than those in control group at recruit year, but these differences were disappeared after 1 year work. Therefore, this suggest that close distance workers may be affected their visual acuity.

최근접 Clustering 방법을 이용한 비디오 스토리 분할

이해만,정규식 崇實大學校 2000 論文集 Vol.30 No.1

We are approaching to an age of mobile multimedia information service which allows mobile network appliances to exchange multimedia information efficiently and effectively. As a core technology for the service, multimedia transcoding which transforms multimedia information to be suited for the appliance environment will be needed. In dealing with video transcoding, we need a good method of video representation for efficient storage and retrieval. One way to do so is to represent a video in a hierarchy of three levels; Frame Shot & Story. In this thesis, we propose a method of story segmentation by joining the neighbor shots, assuming that a video is already segmented into a set of shots. In the proposed method, firstly, we extract a representative (key) frame from each shot by considering its motion information and secondly extract CCV(Color Coherence Vector) features from the extrarted key frame as a visual feature. Thirdly, we apply a nearest neighbor clustering method to the key frames using two measures of visual similarity and temporal similarity. Using clustering results and story repetition property, we spit and merge video shots to make a set of stories. We implement the proposed method using Visual C++ in a IBM PC and perform experiments using movie videos. Experimental results show the effectiveness of the proposed method.

단일클론 항 γ-Glutamyltrasferase(GCT) 항체를 이용한 혈뇌장벽 내 GGT의 분포에 관한 연구

이병규,김명곤,신규만,류총근 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.3

γ-Glutamyltransferase(GGT: E.C. 2.3.2.2.) is a glycoprotein enzyme which is involved in glutathione metabolism and amino acid transport through the plasma membrane. It is distributed widely in several organs including liver, kidney, pancrease and brain. GGTs derived from the brain of Wister rats and BALB/c mice were biochemically purified to a specific activity of 4246.2, 862.1 units per mg of protein, a purification folds 93.7, 43.8 and the final yield 65.8, 44.0% respectively. Electrophoretic pattern of purified GGTs from rats and mice brain shows very similar protein fraction each other. We have produced six monoclonal antibodies(GGT-MAb 1-6) against 2-acetamidofluorene treated rat liver GGT. Using these GGT-MAb 1-6 we performed immunohistochemistry(IHC) to study the distribution of GGY isozymes in normal tissues of rat brain and in ncoplastic tissues of human brain. The results indicated that human brain GGT was localized in pericytes of blood-brain barrier, especially in the blood-rich portion of the brain(e.g. cerebellum of rat, meningioma and craniopharyngioma of human). Therefore these MAbs may be used to evaluate the distribution of GGT isozymes in different tissues.

한국산 노루발풀의 유전적 변이와 집단구조

이인섭,문성기,허만규 慶星大學校 1997 論文集 Vol.18 No.2

Pyrola japonica (Pyrolaceae) is a perennial herbaceous species to Asia. This species reproduces both asexually by underground rhizomes and sexually by seeds. The level of genetic diversity of this species was surveyed using starch gel electrophoresis at putative six enzyme loci from 11 natural populations in Korea. Results from six loci indicated that genetic diversity was high and population divergence was found between genetic identity statistics and geographic distance between populations. Although the range of genetic diversity statistics among populations was unsually large, genetic drift and asexual reproduction did not appear to play major roles in structuring genetic variation. We concluded that the level of genetic diversity maintained within P. japonica populations and the level of population divergence was influenced by its status as forest and mode of gene flow.

근로생활의 질이 구성원의 조직몰입에 미치는 영향에 관한 연구

이종법,이만규,정윤희 東亞大學校 大學院 2005 大學院論文集 Vol.30 No.-

The purpose of this study is to investigate the relation between quality of working life (QWL) and organizational commitment and to determine QWL influences on organizational commitment with a view to improving QWL and organizational commitment. The subjects of the research were 200 workers sampled from seven manufacturing industries and five service industries in Busan and Kyungnam areas. All of them were given a questionnaire consisting of 30 items. Their responses were then analyzed by means of the correlation and regression methods. The results are as follows: Firstly, it is proved that there exists a correlation between QWL and organizational commitment, and that significantly related to organizational commitment are companies' contribution to the society, promotion, human relation in the organization, working environment, and welfare benefits; Secondly, pay is the least influential for working, while promotion is an important factor for high wage workers and long-term employees. This means merit system based on an objective evaluation is necessary. third, harmony with private life has negative influences, and companies' contribution to the society is the most important factor on organizational commitment. Thirdly, service industry rather than manufacturing industry is more affected by QWL factors. This indicates that the collection of members' opinions, their participation in decision making, and sufficient conference are essential to setting up company's goal and managing service industry.

유리 광배근피판을 이용한 하지의 재건

이규윤,김용배,이영만,양순재,박종섭 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.1

Significant soft-tissue defects in the lower extremity including the tibia and the foot are best managed with free skin-muscle of skin-grafted free muscle transfers. While free flap donor sites useful for lower exremity reconstruction include the latissimus dorsi, the rectus abdominis, the gracilis, and the scapular flaps, the latissimus dorsi seems ideal. This muscle has a reliably long vascular pedicle of wide diameter, making microsurgical anastomosis of the thoracodorsal vessels straightforward. Because it is so large, it can cover virtually and size defect. Furthermore, its flat configuration makes it especially easy to fold on itself to that it can be used to closure wounds with complex three-dimensional geometry and dead space. Although the gracilis and rectus abdominis muscles have their roles, in most cases the latissimus dorsi should serve well. We described technical considerations in each case and the advantages of free latissimus dorsi myocutaneous flap on the reconstruction of the soft tissue defect in the lower extremity.

Nifedipine 투여로 증상 호전을 보인 일측성 다발성 피부 모평활근종 1예

이은주,김윤동,이증훈,서기범,박장규,김진만 대한피부과학회 2002 大韓皮膚科學會誌 Vol.40 No.10

We report a case multiple piloleiomyoma in a 50-year-old man. About 30 years ago, multiple red colored firm papules with pain developed on the left lower leg. The skin lesions gradually increased in number and in and size arranging in a group. Some, lesion became more painful after cold exposure. About a few years ago, new lesions developed on the left back and flank abdomen, The histologic findings showed poorly demarcated and were composed of interlacing bundles of smooth muscle fibers with varying amounts of collagen bundles being intermingled. Masson's trichrome and smooth muscle actin stain showed typical leiomyoma, and s-100 stains revealed an increased amount of nerve fibers interlacing within muscle fibers and the surrounding tissue. We diagnosed our case as unilateral multiple piloleiomyoma arising from arrector pilorum muscle. Pain was ameliorated with oral nifedipine alone.

인터넷 광고효과에 관한 조사연구

이태로,최만규,임국환 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

As internet becomes very important communication means for numerous people, a variety of advertisement are beginning to appear on the internet. People can get information about various things and do shopping via internet. This study was carried out to investigate the effect of internet advertisement on various groups. For this study, 200 people were surveyed. The results obtained were as follows: 1. Among the various advertisement types, since most people use e-mail and check e-mail very often, the advertisement through e-mail turned out the most effective advertisement means. 2. The type of advertisement using animation and moving pictures were preferred for most consumers. 3. As for the age groups, young people had the preference for the banner style and old age groups were interested in advertisements including free gift or discount events. 4. As for the favorite hours, the people in the twenties and the thirties preferred evening time(18:00 to 24:00) and people in the forties and fifties afternoon(12:00-18:00). 5. Male consumers showed preference (or the advertisement including free service programs and female consumers preferred the advertisement including discount programs or gifts.