한국내 일본사초의 집단 유전 구조

허만규,허홍욱 한국유전학회 1998 Genes & Genomics Vol.20 No.4

Genetic diversity and population structure of seventeen Carex hondoensis (Cyperaceae) populations in Korea were determined using genetic variation at 23 allozyme loci. The source of this species in Korea is native to Japan. The percent of polymorphic loci within the enzymes was 52.2%. Genetic diversity at the species level and at the population level was high (Hes = 0.170; Hep = 0.156, respectively), whereas the extent of the population divergence was relatively low (G_(ST) = 0.043). F_(IS), a measure of the deviation from random mating within the 17 populations, was 0.191. An indirect estimate of the number of migrants per generation (Nm = 5.51) indicates that gene flow is high among Korean populations of the species. Analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Mean genetic identity between populations was 0.993. This low level of genetic differentiation (G_(ST) = 0.043) and high gene flow among populations (Nm = 5.51) may be undoubtedly contributed to the spread of this species in Korea. In addition, it is highly probable that directional toward genetic uniformity in a relatively the homogeous habitat is thought to be operated among Korean populations of C. hondoensis.

Developed EFG법으로 성장시킨 $YVO_{4}$ 및 Nd:$YVO_{4}$ 단결정의 광학적 특성

허만규,서수정,윤대호,M.A. Ivanov,V.V. Kochurikhin 한국결정성장학회 2001 한국결정성장학회지 Vol.11 No.4

Developed Edge-defined film-fed growth (EFG)법으로 $YVO_{4}$ 및 Nd:$YVO_{4}$ 단결정을 성장하였으며 성장된 결정의 광학적 특성을 측정하였다. $YVO_{4}$ 및 Nd:$YVO_{4}$ 단결정 모두 투명하였으며, 결정성장 동안 융액 표면 온도의 균질 및 meniscus의 안정화로 고품질의 결정을 얻을 수 있었다. 투과 및 흡수 스펙트럼 측정 결과에서 $YVO_{4}$ 단결정은 340 nm에서 1000 nm까지 대체로 넓은 영역에서 높은 투과율이 나타났지만, Nd:$YVO_{4}$ 단결정은 532, 593, 753, 807, 888 nm의 특정한 영역에서 흡수 peak들이 나타남을 획인할 수 있었다. 또한 Photoluminescence (PL) 스펙트럼을 측정한 결과, 800~900 nm의 영역에서 에너지 방출을 관찰할 수 있었다. $YVO_{4}$ and Nd:$YVO_{4}$ single crystals have been grown developed Edge-defined film-fed growth (EFG) method and the crystals were measured on optical properties. $YVO_{4}$ and Nd:$YVO_{4}$ single crystal were transparent, high quality due to homogeneity of surface temperature of the melt and stability of meniscus during crystal growth. In transmittance and absorption spectra, Nd:$YVO_{4}$ single crystals had absorption peaks at wavelengths of 532, 593, 753, 808, 888 though $YVO_{4}$ single crystal had a broad transmittance at wavelength ranging from 340 to 1000nm. Also, Nd:$YVO_{4}$ single crystals had emissions of energy at range of 800~900 nm in photoluminescence (PL) spectrum.

한국내 조릿대의 교배계에 대한 유전학적 연구

허만규,노광수,Huh Man Kyu,Roh Kwang Soo 한국생명과학회 2005 생명과학회지 Vol.15 No.1

조릿대(S. borealis)는 다년생 초본으로 한국, 일녈, 중국의 일부에 제한적으로 분포한다. 이 종은 자가 및 타가수분에 의한 혼합번식 형태를 취한다. 전분 전기영동을 이용한 12개의 대립 유전자좌위에서 종의 교배계를 조사하였다. 교배계에서 타가 및 자가 수분, 그리고 줄기의 변형으로 영양번식이 이루어지는 매우 복잡한 혼합 방식의 교배 양식은 제한된 분포, 고산식생대 등의 환경에서 생존할 수 있는 조릿대의 생존전략과도 관련이 있을 것으로 보인다 또한 일부 자생지 파괴에 대한 유효집단의 감소가 일어나 생물자원 확보를 위한 자생지 보존이 필요하다 Sasa borealis Makino is distributed in East Asia such as Korea, Fushun in China, and Japan. Especially the species is only found in the high altitude (above 600 m) at mountain of cold regions including The Korean Peninsula. The level of genetic diversity and population structure of this species was surveyed using starch gel electrophoresis at putative five enzyme loci from three natural populations in Korea. Results from twelve loci indicated that genetic diversity was low. In addition, analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and loci. The reasons for the deficit of heterozygosity may be partly considered inbreeding, small population sizes, and mating of demes. S. borealis can reproduce extensively by producing rhizomes and potentially by sexually produced seeds. Rhizomes are generally long, prostate stems rooting at the nodes.

Ecological and Genetic Biodiversity of Corbicula leana in the Nakdong River and the Nam River

허만규,이복규,김병기,허윤성,이학영 한국생태학회 2005 Journal of Ecology and Environment Vol.28 No.2

The eleven local samples of six species including Corbicula leana examined in this study werecollected from the Nam River and the Nakdong River in Korea. Buzas's new formula was used to evaluate the ecological biodiversity among eleven populations and six species. In addition, enzyme electrophoresis was used to genetic diversity within and among populations of C. leana. The upper populations of the river have fewer species than the middle and low populations of this river. The genetic diversity trends to increase from the source upstream.

Comparison of Genetic Diversity and Population Structure of Kalopanax pictus (Araliceae) and its Thornless Variant Using RAPD

허만규,Sang Duk Jung,Heung Kyu Moon,Sea-Hyun Kim,Jung Sook Sung 한국약용작물학회 2005 한국약용작물학회지 Vol.13 No.2

Kalopanax pictus is a long-lived woody species mostly distributed in East Asia. K. pictus has been regarded as medically and ecologically important species in Korea. Thornless castor aralia variant, local name "Cheongsong" is an endemic to Cheongsong province in Korea. Random amplified polymorphic DNA (RAPD) was used to investigate the genetic variation and structure of Korean populations of two species. A high level of genetic variation was found in six K. pictus populations. Twelve primers revealed 49 loci, of which 29 were polymorphic (59.2%). Nei's gene diversity for K. pictus and K. pictusvariant were 0.119 and 0.098, respectively. Mean of genetic diversity in K. pictus was higher than average values for species with similar life history traits. The asexual and sexual reproduction, perennial habitat, and longevity are proposed as possible factors contributing to high genetic diversity. An indirect estimate of the number of migrants per generation (Nm = 0.857) indicated that gene flow was not extensive among Korean populations of K. pictus. It is suggested that the isolation of geographical distance and reproductive isolation between K. pictus and K. pictus variant populations may have played roles in shaping the population structure of this species.

Genetic Diversity and Population Structure of Alder, Alnus japonica Using AFLP Markers

허만규,이주경 한국유전학회 2004 Genes & Genomics Vol.26 No.3

Genetic Diversity and Population Structure of Acanthopanax sessiliflorus Using Random Amplified Polymorphic DNA Markers

허만규,허홍욱,최주수 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.3

Acanthopanax sessiliflorus (Araliaceae) is a long-lived woody species primarily distributed throughout East Asia. This species is regarded as medically and ecologically important in Korea. We used random amplified polymorphic DNA to investigate its molecular variation and genetic structure in samples from Korean populations (five wild and four cultivated). The analysis of 22 primers revealed 92 loci, of which 76 were polymorphic (82.6%). Total genetic diversity at the species level was 0.316. The mean gene diversity for cultivated populations was lower than that for natural (wild) populations. Sexual reproduction modes, perennial habitat, and longevity were possible factors contributing to such high diversity. An indirect estimate of the number of migrants per generation (Nm= 0.367) indicated that gene flow was very low among these Korean populations.

RAPD마커를 이용한 가시오갈피의 기원판별 및 유전적 다양성

허만규,최영현,최병태,김경철,Huh Man Kyu,Choi Yung Hyun,Choi Byung Tae,Kim Gyeong Cheol 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

Acanthopanx senticosus (Araliaceae) is a long-lived woody species primarily distributed throughout East Asia. This species is regarded as medically and ecologically important woody plants in Korea. To identify the variation of the RAPD patterns between domestic and foreign A. senticosus species, 22 random primers were applied to Korean A. senticosus and A. senticosus for. inermis, Chinese and Russian A. senticosus. Six primers of them could be used to discriminate the origins and 58 polymorphisms among 92 scored DNA fragments. Six bands are specific for Korean A. senticosus and A. senticosus for. inermis. Especially, three primers, OPD04, OPD11 and OPE10, were useful to differentiate between domestic and foreign Acanthopanax species. RAPD analysis was a useful method to discriminate among A. senticosus populations or accessions and Korean accessions are distinct genetically.

한국내 삽주의 유전적 다양성과 집단구조

허만규,성정숙,박춘근,박희운,성낙술,문성기,허홍욱 한국약용작물학회 2002 한국약용작물학회지 Vol.10 No.1

한국내 분포하는 다년생 초본인 삽주 집단의 유전적 다양도와 집단구조를 조사하기 위해 전분 전기영동으로 분석하였다. 15 대립유전자좌위당 9개 좌위에서 다형현상(60.0%)을 보였으며, 유전적 다양도는 종수준에서 0.144로 높은 반면 집단수준이 이보다 약간 낮았다. 삽주의 유전적 다양도는 대부분 집단내에 존재하였고 유사한 생활양식을 가진 다른 식물종에 비해 높았다. 그 이유로는 유성생식, 다년생, 다산 등에 기인한다. 집단간 분화는 약 13%였고 지리적 거리와 유전적 거리의 상관은 높았다(r=0.65). 그럼에도 불구하고 일부 격리된 집단은 유효집단크기를 가지지 못하여 이형접합체의 결여가 유의성을 보여 다양도가 높은 집단의 보존이 요망된다. The study of genetic diversity was carried out in Atractylodes japonica KOIDZ. Although this species has been regarded as medically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of eight Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level (Hes=0.144), whereas, that of the population level was relatively low (Hep=0.128). Nearly 87% of the total genetic diversity in A. japonica was apportioned within populations. The sexual reproduction, high fecundity, and perennials are proposed as possible factors contributing to high genetic diversity. The indirect estimated of gene flow based on Gst was 1.69.

침수지에서 식물 생태 천이와 군집변화

허만규,최주수,문성기,Huh, Man-Kyu,Choi, Joo-Soo,Moon, Sung-Gi 한국생명과학회 2006 생명과학회지 Vol.16 No.7

Community structure varies not only in space but also in time. We stand in one position which located at Sinhung-ri, Sacheon-ci and observe the flora of area as time passes. This area begins after disturbance on sites where organisms are already present like secondary succession. In 2001, there are invaded by Trapa japonica, Salvinia natans, and Potamogon cristatus. Four or five years later, this abandoned areas support of clover, pine, and oak, resulting in a shifting pattern of species dominance and diversity through time. Patterns of community structure such as species composition and ecological diversity have shown through seasons and five years, that is the topic of this study. The values of turnover (TO) were changes through time intervals at three regions. The gradual and seemingly directional change in the structure of the community through time from aquatic like to field. Thus, we can observe the process of secondary succession at Sinhung-ri in the very short time.