BMB Reports : Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21^Cip1 and p27^Kip1

( Hyang Jee ),( Su Hyung Lee ),( Jun Won Park ),( Bo Ram Lee ),( Ki Taek Nam ),( Dae Yong Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.1

Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and p21^Cip1 and p27^Kip1 expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting. Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly. G1 arrest, up-regulation of cell cycle-regulatory proteins p21^Cip1 and p27^Kip1 was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins. [BMB Reports 2013; 46(1): 25-30]

비강 및 비인두에 발생한 도립유두종과 편평상피암종의 p53단백 및 세포증식능에 관한 연구

한주호,윤기중,이재규,신대균,박근호,조향정,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

The inverted papilloma and squamous cell carcinoma are common neoplasia in the sinonasal cavity and nasopharynx, but the incidence of these tumors are very low and the study on the oncogenesis or biological activity of the tumor cells are not well known. This study was designed to evaluate the oncogenic roles of the p53 gene and the proliferative activity of the tumor cells in the inflammatory polyp, inverted papilloma and squamous cell carcinoma. The experiment was carried by the immunohistochemical stains about the p53 protein, PCNA and Ki-67, histochemical stain about the AgNORs. and flow cytometric analysis about the DNA ploidy using the formalin fixed paraffin embedded tissues. The frequency of the expression of p53 protein was 0%(0/16 cases) in the inflammatory polyps, 18.8% (3/16 cases) in the inverted papillomas, and 87.5%(14/16 cases) in the squamous cell carcinomas. The labelling index(%) of the PCNA and Ki-67 was 5.3% and 3.3% in the inflammatoy polyps, 29.6% and 25.2% in the inverted papillomas, and 51.9% and 36.8% in the squamous cell carcinoma. The expression of the PCNA and Ki-67 was distributed in the periphery of the tumor islands of the inverted papilloma and was distributed in the both of center and periphery of the tumor islands of the squamous cell carcinoma. The number of nuclear AgNORs was increased in the order of inflammatory polyps (0.96), inverted papillomas(1.34) and squamous cell carcinoma(2.09). The frequency of the DNA aneuploidy was 0%(0/16 cases) in the inflammatory polyps, 18.8%(3/16 cases) in the inverted papillomas. and 12.5%(2/16 cases) in the squamous cell carcinomas. Above results indicates that the changes of the p53 gene and proliferative activity of the tumor cells are involved on the oncogenesis and the biological activity of the inverted papilloma or squamous cell carcinoma in the nasopharynx and sinonasa cavity.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

中風病辨證診斷標準에 의한 臨床的 硏究

任長信,文祥官,高昌男,權正男,曺基湖,金永錫,裵亨燮,李京燮 경희대학교 1997 慶熙醫學 Vol.13 No.3

A2024와 SM45C 마찰용접의 열전달 해석

이상윤,고준빈,명태식,김기길,강형모 大田産業大學校 2001 한밭대학교 논문집 Vol.18 No.-

The heat transfer mechanism initiating the friction welding is examined and a transient three dimensional heat conduction model for the welding of two dissimilar cylindrical metal bars is investigated. The cylindrical metal bars are made of materials made of A2024 and SM45C. Numerical simulations of heat flow are performed using the finite volume method. Respectively, Commercial FLUENT code is used in the heat flow simulation and maximum temperature and distribution of temperature are calculated. Temperature of friction welded joining face is compared with the temperature distribution measured by experiment and numerical simulation. The maximum temperature of friction welded joining face is lower than melting point of A2024-T6 aluminum alloy using insert metal. The temperature distribution of friction welded joining face with insert metal is more uniform than that of without insert metal

정신과 외래환자의 약물치료 반응과 심박동 변이의 변화 양상

이상규,이홍석,김인주,김형태,김도훈,손봉기 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.6

Objectives : The purpose of this study was to investigate the change of heart rate variability after antidepressant treatment in depressed or anxious patients. We tried to find the usefulness of heart rate variability as a predictive marker of response to pharmacological treatments in the psychiatric field. Methods : Forty-seven patients with major depression, anxiety disorder, and somatoform disorder were treated over 4 weeks with antidepressants and were then classified as responders or nonresponders according to Hamilton depression scales or CGI scores. The HRV variables were measured and compared among the responders/nonresponders and the matched normal control. Results : The responders group exhibited low heart rate, low PSI, high SDNN, RMSSD, HRV index, NN50, pNN50, LNTP, LNLF, and LNHF compared with the nonresponders after 4 weeks antidepressant treatment. However, no significant differencein HRV vanables was observed between the responders group after treatment and the nonnal control group and the responsegroup after treatment. Conclusion : These results indicate that HRV variables would are normalized after successful antidepressant treatment and it that HRV has some possible uses as a predicting marker of antidepressant response.

敎科課程을 中心으로 한 韓日農學系(園藝·食品·獸醫)大學의 敎育現況 調査

李愚升,孫泰華,尹衡植,毛麒喆 경북대학교 1982 새마을 硏究論叢 Vol.2 No.-

The authors studied on the curicullum between Korea and Japan in the deaprtments of horiculture, food technology and veterinary. The results are as follows. 1. In the department of horticulture, professional education period was 3 years in Korea while 2 or 2.5 years in Japan. These results were compared among 3 colleges of Korea and Japan. Credits for graduation was 48-52% for propessional education with submajoring subjects and 45% when majoring subject in Korea, but 54-61% in Japan. Credits for required subjects were 17-29% among credits for graduation in Korea while 28-29% in Japan. 2. Cultural education period is almost 1 year in Korea, but different in Japan. Credits for graduation are as follows; Cultural education is 36-37%, majoring fields 15% and the others 12-13% in Korea while 43-46% for cultural education, 54-57% for majoring field in Japan. Differences were shown among 3 colleges dealt with the department of food technology by locational characteristics in Japan. 3. In the department of Veterinary, collage curicullums were not fixed for the students because of transitional period. Required subjects among 4 colleges were shown as the same tendency in Korea.

非線形 理論에 依한 채터 解析

이형식,정준기,박태형 연세대학교 산업기술연구소 1983 논문집 Vol.15 No.2

The chatter analysis has been studied by many authors using non-linear. In this study, the stability chart obtained from experimental work with a lathe was qualitatively compared with the result of chatter analysis by non-linear theory. Experimental results verify that the stability of the machining process is affected by the width of cut in the following manner. As the chip width is increased from zero with the rotational speed of the workpiece kept constant, three ranges are distinguished; (a) unconditionally stable range, (b) conditionally stable range, (c) unstable range. In the conditionally stable range the system is stable for small disturbances but unstable when these exceed a certain level. When the chatter occurs, the amplitude initially increases and stabilizes itself at a finite level. This phenomenon agrees well with the analysis of non-linear theory qualitatively.

TGA를 이용한 가교폴리에틸렌의 방사선 열화 평가

이청,김기엽,류부형,임기조 한국안전학회 2003 한국안전학회지 Vol.18 No.2

Radiation degradation of crosslinked polyethylene(XLPE) was investigated using thermogravimetric analysis (TGA), The results of TGA were compared with FT-IR, melting temperature, oxidation induction time, and elongation al break on the XLPE exposed by γ-ray. 5% decomposition temperature of γ-ray irradiated XLPE showed similar tendencies with the case of elongation at break. Both properties agreed below 1000 kGy, however, did not show any remarkable characteristics above 1000 kGy, these properties can be useful to evaluate the radiation degradation of XLPE for only low irradiated region. Above 1000 kGy, the thermal decomposition activation energy showed decreased, on the contrary, increasing below 1000 kGy. Compared with FT-IR spectrum of irradiated XLPE, it was confirmed that the oxidation reaction was still occurring below 1000 kGy. Radiation degradation of XLPE was dependent upon the irradiation doses, TGA can be a useful tool to evaluate the degradation.

Acanthamoeba의 한국형 동정 및 SAM-synthetase와 Ubiquitin의 생활사 특이적 발현

이기현,최호형 公州大學校 基礎科學硏究所 1996 自然科學硏究 Vol.5 No.-

Acanthamoeba는 자유생활을 하며 전세계적으로 모든 환경에서 존재한다. 한국형 Acanthamoeba는 공주산성에서 발견되지만 cyst의 크기가 다양하여 더 자세한 특징을 서술할 필요가 있다. 활성아메바는 cyst-inoculation 시작 3일후에 관찰되고 cyst들은 11일째 PYG 배지에서 관찰되며 세포밀도는 9일째 가장 높다. SAM-synthetase mRNA와 ubiquirin mRNA 합성은 포낭시기동안 감소되고 SAM-synthetase mRNA는 ubiquitin mRNA보다 더 빨리 감소된다. 이런 결과는 SAM-synthetase mRNA와 ubiquitin mRNA의 농도감소가 전사 단계에서 조절된다는 것을 시사한다. Acanthamoeba is a free living amoeba that is present in all types of environments throughout the world. A Korean type Acanthamobea was identified at Sang-sung Park in Kong-ju city, but it needs more chracterization because size of cyst was various. Activated amoeba were observated at day 3 from cyst-inoculation, and cysts were detected at day 11 in PYG-media and cell-density was most high at day 9. Synthesis of SAM-synthetase mRNA and ubiquitin mRNA were decreased during encystment, and SAM-synthetase mRNA was decreased more rapidly than ubiquitin mRNA. This results suggest that decline of concentration of SAM-synthetase mRNA and ubiquitin mRNA were presumed that were regulated in transcription level from the compared with report of Feldman(1991), Moura(1992) and Tomlinson(1991) that concentration of SAM-synthetase and ubiquitin were decreased during encystment in Acanthamoeba.