봉독약침이 류마티스 관절염 환자의 기능회복 및 삶의 질에 미치는 영향

이상훈,이현종,박상민,김수영,박재경,홍승재,양형인,이재동,최도영,김건식,이두익,이윤호 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective: To evaluate the effects of bee venom acupuncture(BVA) on the rehabilitation and quality of life in rheumatoid arthritis(RA) patients Methods: Patients with RA were treated with the BVA therapy twice a week for 3 months. Tender joint counts, swollen joint counts, morning stiffness, Erythrocyte Sedimentation Rate(ESR), C-reactive protein(CRP), patient global assessment, physician global assessment, Korean health assessment questionnaire(KHAQ) were estimated and analyzed before and after BVA therapy. Results: Tender joint counts, swollen joint counts, morning stiffness showed significant decrease after BVA therapy. But, as acute inflammatory reactants, ESR showed no significant difference and CRP showed significant increase after BVA therapy. Patient global assessment physician global assessment, and KHAQ index showed significant improvement after BVA therapy. Conclusions: BVA therapy can improve rehabilitation and health-related quality of life RA patients as well as clinical symptom and signs. Further study is required in more population with large scale including acute inflammatory reaction of BVA therapy.

뇌졸중 후 중추성 통증 환자에 대한 동서협진이 진통과 재활에 미치는 영향

이현종,김수영,이상훈,서동민,이두익,김건식,이재동,이윤호,양형인,박재경,최도영 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Purpose : In order to study the effectiveness of East-West pain treatment on central poststroke pain(CPSP), we evaluated its effect on alleviation of pain and rehabilitation of CPSP Patients who were treated with electroacupuncture and west pain treatment for four weeks. Methods : Twenty four patients diagnosed by their pain characteristics of central pain from stroke were treated with sympathetic nerve block, gabapentin, amitriptyline, and electroacupuncture for four weeks. Pain intensity through the visual analogue scale(VAS), and improvements of mobility and rehabilitation through the modified Barthel index(MBI) and Rankin scale(RS), respectively, before and after pain treatment were also assessed. Results : VAS pain scores were significantly improved from 7.7±1.7 to 4.4±2.0 with pain treatment(p<0.05). In accordance with improvement of pain scores, RS and MBI scores were also improved from 2.88±0.95 to 2.13± 1.01 and from 83.0± 16.9 to 94.7±9.5(p<0.05), respectively, with pain treatment(p<0.05). Conclusions : It was suggested that the active pain treatment was contributed to the rehabilitation of CPSP patients, resulting in improvement of quality of life of CPSP patients. Futhermore, East pain treatment in combination with West pain treatment may be useful modality to alleviate CPSP.

무작위 대조 이중맹검 시험을 통한 봉독 약침의 류마티스 관절염 치료 효과 연구

이상훈,홍승재,김수영,양형인,이재동,최도영,이두익,이윤호 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective : This study was performed in order to evaluate the effect of bee venom therapy on rheumatoid arthritis by randomized controlled double blind method. Method : RA patients were recruited and divided into an experiment group and a control group by random selection. As a double blind test, the experiment group was treated with bee venom injection on acupoints, and the control group was treated with normal saline injection on acupoints twice a week for 8 weeks. Tender joint count, swollen joint count, morning stiffness, pain, health assessment questionnaire, ESR. and CRP were estimated and analyzed at baseline, and at 1 month and 2 months after bee venom therapy Results : Compared to the control group, the experiment group showed significant decrease in tender joint count swollen joint count, morning stiffness, and health assessment questionnaire after 2 months. Pain, ESR and CRP showed significant decrease in the experiment group after 1 & 2 months. Conclusions : These results suggests that bee venom therapy could be an effective method in the treatment of patients with rheumatoid arthritis.

봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향

이상훈,이현종,백용현,김수영,박재경,홍승재,양형인,김건식,이재동,최도영,이두익,이윤호 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective In order to study the effects of bee venom(BV) on the pain, edema, and acute inflammatory reactant of rheumatoid arthritis(RA) patients. Methods Patients with RA who met the ACR(American College of Rheumatology) 1987 revised criteria for the diagnosis of RA were treated with the BV therapy twice a week for 3 months. Tender Joint counts, swollen joint counts, Visual analog scale(VAS), morning stiffness, ESR. C-reactive protein(CRP) were analyzed before and after BV therapy. Results The results as follows. 1. Tender joint counts in patients after BV therapy were significantly lower than those before BV therapy(9.0±7.9 vs 15± 11.4, p=0.002). 2. Swollen joint counts of the patients after BV therapy were significantly lower than those before BV therapy(50±61 vs 15±23, p=0.001). 3. VAS in patients after BV therapy was significantly lower than those before BV therapy(608± 17.6 vs 380± 159, p=0.000). 4. Duration of morning stiffness in patients after BV therapy was significantly reduced compared with that before BV therapy(119.1± 112.6 min vs 59.0±89.7 min, p=0.009). 5. ESR and CRP were not significantly changed before and after BV therapy, suggesting BV itself could make inflammatory reaction as well as therapeutic effect. Conclusions BV therapy improved tender joint counts, swollen joint counts and duration of morning stiffness in this study, and further study is needed on long-term effect of BV therapy.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

PLZT 세라믹을 이용한 SAW Filter의 특성

이수대,이찬구,안형주,문준규 경남대학교 신소재연구소 2003 신소재연구 Vol.15 No.2

현재 사용되고 있는 SAW 필터 기관물질 중 세라믹 기관은 다공정과 생산에 따른 물성의 재현성 부족을 제외한다면 단결정 기관에 비해 생산단가의 저렴화, 생산공정의 단순화 및 대량생산의 측면에서는 훨씬 경쟁력이 있다. 따라서 본 연구에서는 고온 압축성형법에 의해 1235℃의 온도와 200㎏/㎠의 압력에서 30시간동안의 제조조건에서 조성비 x/65/35(x=5-8)인 PLZT 세라믹 기관들을 제조하여 SAW필터로써의 응용가능성을 살펴보았다. 각 조성별 유전을, SAW 전파 속도, 기계결합계수 등을 측정 비교한 결과 SAW 필터 기관물질로 가장 적합한 조성비는 7/65/35였다. 이 조성비로 제작한 SAW 필터에서 상대적으로 양호한 출력 특성을 얻었고 단결정 기관으로 제작한 SAW 필터에 버금가는 특성을 확인할 수 있었다. The single crystal substrate has been the industrial standard for the design of SAW filters for a long time. However, the ceramics substrate had more simple fabricated process than the crystal. In this study, it was attempted that single crystal was substituted for PLZT(La/Zr/Ti = x/65/35, x=5∼8 at. %) ceramics. The ceramics substrate was prepared by hot-pressed method at temperature 1235C, 200㎏/㎠, 30hrs. The real permittivity, SAW velocity and mechanical coupling factor of PLZT ceramics were measured for a different La mol% content. In the results, we could observed that 7/65/35 composition SAW filter had a good characteristics of frequency response.

海外留學指導硏究方案樹立을 위한 基礎硏究

李炯國,安正秀 慶熙大學校 1980 論文集 Vol.10 No.-

수온 변동 자극이 양식 넙치, Paralichthys olivaceus의 비특이적 생체 방어에 미치는 영향

이덕찬,김도형,김수미,강명석,홍미주,김현정,박수일 한국어병학회 2002 한국어병학회지 Vol.15 No.2

본 연구는 넙치에 대한 수온 변동 자극의 영향을 생체 방어적인 측면에서 검토하고자 하였다. 수온 변동 자극은 매일 18℃에서 25℃까지 상승과 하강을 반복하는 방법을 사용하였으며 그 기간은 30일간 지속하였다. 실험어는 온도변화를 주기 시작한 후 28일째까지 무작위로 채포하여 말초혈액 중의 백혈구 수, 전혈의 식작용능, 활성산소, 환원능, chemiluminescence(CL) response 및 lysozyme용균능과 같은 각종 비특이적 생체방어와 관련한 실험을 수행하였다. 수온 변동 자극을 주기 시작한 후 1일째부터 시작하여 1주일 이내에 약 18%의 넙치가 폐사하였으며 이후에는 대조구와 유사하였다. 또한, 수온 변동 자극을 준 실험구의 호중구 수는 2주째에 유의적인 증가를 보였으며, 림프구 수는 2일과 3일째에 감소하였으나 1주째부터 대조구와 유사한 수준으로 회복되었다. 실험구에서는 식작용 결과 식균율과 식균지수에서 이물질 투여 후의 반응이 늦게 나타났다. 식세포의 활성을 조사한 NBT reduction 실험에서는 실험 기간 동안 대조구와 유사한 경향ㅇ르 나타내었다. 또한, CL response의 경우, 실험구는 온도 변동 자극초기에 대조구에 비하여 유의적으로 낮았다. 그리고, 식세포가 분비하는 용균성 효소인 점액 내 lysozyme의 활성은 실험 기간 동안 실험구에서 낮게 나타나는 경향을 보였다. 이러한 여러 가지 비특이적 생체 방어 반응의 저하 현상은 수온 변동 자극 초기에 나타났으며, 1주일 정도 지속하면 그 환경에 적응하는 것을 알 수 있었다. 그리고 이러한 현상은 이들이 초기 폐사 발생과 어떤 상관 관계에 있는 것으로 생각되었다. This study was performed to know the effects of stress nduced by the daily fluctuation of water temperature fro 18? to 25? up and down for 30 days on the defence mechanism of olive flounde,r Paralichthys olivaceus. To make clear the temperature stress on the defense mechanism of the tested fish, several factors of immune response such as counting of leucocyte appearance in periopheral blood, phagocytic activity in whole blood cells, nitroblue tetrazolium(NBT) reduction, chemiluminescence(CL) response, and lysozyme activity were investigated at 28 days after giving the change of water temperature. The fish was controlled under the none feeding condition during experimental period. Mortality of the tested fish was rapidly increased up to 22% within the first one week of the experimental period without any additional stress factors. The number of neutrophil of peripheral blood in the tested group was significantly higher than the control group at the 2nd week, but the number of lymphocyte was significantly lower than the control group at the 1st and 3rd day of the experimental period, respectively. In the NBT reduction test, the activity of macrophage in the control group fish was the highest on the 7th day while that in the tested group was on the 3rd day. Also, the phagocytosis of tested group against formalin killed cells was retarded compared with the control CL response of the tested group was significantly lower from 2nd to 5th day of the experimental period than the contro. The lysozyme activity of tested group was remained higher during the experimental period than the contro. Even through the tested fish showed different results in some non-specific factors of immune responses between tested and control group fish, olive flounder seems highly adaptable in repeated water temperature change in condition after one week under the given temperature fluctuation range

고립성 직장결핵 3예

이동수,정강욱,황도연,이형화,김대현,김영성,곽동협 대한내과학회 2003 대한내과학회지 Vol.64 No.1

저자들은 장결핵 중 발생 부위상 드문 곳으로 알려진 직장에 발생한 고림성 결핵을 3예 경험하였기에 문헌고찰과 함께 보고하는 바이다. Isolated rectal tuberculosis commonly involves sigmoid, ascending, or transverse colon. Rectal involvement in tuberculosis is uncommon and poorly characterized. Isolated rectal tuberculosis was defined as focal lesions of rectum in the abscence of demonstrable lesions in the small and large bowel. Diagnosis of rectal tuberculosis was based on characteristic endoscopic appearance of lesions, histopathologic feature of tuberculosis in biopsy material and response to antitubercular therapy. Three patients with isolated rectal tuberculosis were seen at Kwak's hospital. The lesions observed was classified according to macroscopic morphology as follows: ulcerative, hypertrophic and ulcero-hypertrophic. Clinical manifestations were non-specific and consists of rectal bleeding, fever, weight loss, constipation and abdominal pain. One of the three patients had coexisting tuberculous pleurisy. Response to antitubercular chemotherapy was good. (Koeran J Med 64:96-100, 2003)

전도성 고분자 센서 어레이를 이용한 휘발성 유기 화합물 가스 인식

이경문,주병수,유준부,황하룡,이병수,이덕동,변형기,허증수 한국센서학회 2002 센서학회지 Vol.11 No.5

휘발성 유기 화합물 가스(Volatile Organic Compounds)를 인식하고 분석하기 위하여 전도성 고분자 센서어레이를 이용한 시스템을 제작하였다. Polypyrrole와 Polyaniline을 화학중합법으로 센서에 전도성고분자막을 형성하였고 이를 통해 VOC 검지용 센서 어레이를 제작하였다. 센서어레이로부터 측정되는 다차원 데이터는 주성분분석법(PCA)과 RBF(Radial Basis Function Network)을 이용하였다. 제안된 시스템으로 VOCs 가스를 인식하는데 있어서 RBF Network이 PCA방식보다 더욱 효율적인 것으로 판단되었다. We fabricated gas recognition system using conducting polymer sensor array for recognizing and analyzing VOCs(Volatile Organic Compounds) gases. The polypyrrole and polyaniline thin film sensors which were made by chemical polymerization were employed to detect VOCs. The multi-dimensional sensor signals obtained from the sensor array were analyzed using PCA(principal component analysis) technique and RBF(radial basis function) Network. Throughout the experimental trails, we confirmed that RBF Network is effective than PCA technique in identifying VOCs.