사립체 DNA 뉴클레오타이드 3243 A→G 돌연변이 [MTTL1^*MELAS3243G]에 의한 MELAS 증후군

박기형,김승현,이영주,김희태,김주한,김명호,박성섭 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

양식산 터봇, Scophthalmus maximus에서의 Irido-like virus 감염

김위식,김기홍,김춘섭,김영진,정성주,정태성,신이치, 키타무라,마모루, 요시미즈,오명주 한국어병학회 2003 한국어병학회지 Vol.16 No.3

본 연구는 병리조직학적 및 전자현미경적 방법을 통해 양식산 turbot에서 발생하는 irido-like virus의 감염증을 소개하고자 한 것이다. 2003년 6월 전북 고창 소재 육상수조에서 양식중인 turbot치어에서 50∼70%의 대량폐사가 발생하였다. 병어는 사료섭취가 저하되고 힘없이 유영하다 폐사되었으며, 죽기 직전의 어류는 복부가 팽만되고 안구가 돌출되며 체색이 옅어지는 증상을 보였다. 해부학적 조사 결과, 아가미 빈혈과 간이 퇴색되어 있었으며, 특히 비장조직이 심하게 비대되어 있었다. 병어에 대한 조직학적 검사에서 비대 세포를 특징으로 하는 특이 병변이 주로 신장과 비장의 조혈조직에서 관찰되었으며, 아가미, 심장, 위, 장, 간, 췌장, 지방 조직에서도 관찰되었다. 그리고 전자현미경적 관찰 결과, 비대 세포의 세포질내에서 육각형의 지름이 136∼159 ㎚인 Irido-like virus를 관찰할 수 있었다. The high mortality of cultured juvenile turbot, Scophthalmus maximus occurred in Gochang on June, 2003. The diseased fish was lethargic with reduced feed intake. Grossly, these fish showed pale body, abdominal extension and exophthalmia. The dominant internal gross features of diseased fish were severely enlarged spleen, pale gills and or liver. Diseased fish histologically showed basophilic enlarged cells in the kidney, spleen, gill, heart, stomach, intestine, liver, pancreas and adipose tissue. Transmission electron microscopy (TEM) reveled hexagonal virions in the cytoplasm of necrotic cells. The viral particles lead a central electron-dense core and an electron translucent zone, and were 136-159 nm in diameter. These results suggest that the virus belonging to the iridoviridae was responsible for the mortality of cultured juvenile turbot.

국내의 양식산 무지개송어 성어로부터 분리된 IHNV의 G protein gene에 대한 연구

김기홍,김위식,김춘섭,김영진,정태성,신이치, 키타무라,정성주,오명주 한국어병학회 2003 한국어병학회지 Vol.16 No.3

An Infectious hematopoietic necrosis virus strain (IHNV-RTK) was isolated from cultured rainbow trout at Kumi in Korea during 2000 and 2001. In the RT-PCR amplification with the specific primer set designed from IHNV G protein region, a 540 bp PCR product was amplified from the RTK strain. The RTK strain showed higher sequence homology with the published IHNV G protein genes (RB-76, LR-73, Col-85, and Carson-89).

루프 미러로 구성된 기준팔을 가지는 파장 스위핑 OCT 구현

김훈섭,남궁명우,이정렬,엄진섭 江原大學校 産業技術硏究所 2007 産業技術硏究 Vol.27 No.B

As a reflector of reference arm in OCT systems, a coated fiber reflector has mostly used. Instead, this paper has realized the swaept source optical coherence tomography system using a loop mirror within a reference ann. Through the expehments for a tilted thin glass plate, we found that the loop mirror is better than the coated fiber typed mirror for obtaining the clear image.

廣域光陽灣地域의 機能配置에 關한 硏究

金明洙,盧春變,趙南勳 순천대학 새마을연구소 1987 새마을硏究論文集 Vol.3 No.-

The wide area of kwangyang Bay will be, in the 2000s, developed into a met­roploise with the main functions such as those of manufacturing industry, shipp­ing industry and harbor works, tourist and service industry. Such a development of the said area but to the development of national economy through attempting a balanced development of our country and solving the social disadvantages caused by centralization and accumulation. With this development of the area of kwanghang Bay, Sunchon-city not only as a center of culture, education and distribution but also as a comprehensive resea-rch complex of agriculture and industry should carry out a head function in the wide area of kwnagyang Bay. It should also establish Yeochon-city as stronghold of tourism and marine transportation and make the city at the rear of kwangyang Bay carry out the synthesized function as both a port and industrial city. Yeochon city should be developed into a residential area in the back of Yeo-chon Industry Complex : the city in the back of Yulchon should be developed in to the city with the function of a garden city. In addition, with the view of meeting the great increase in tourism demand, recreation facilities should be provided, and a closer connection with the sight-seeing areas of Jiri Mountain and Hanryosudo should be also built up. For the rational distribution and the full use of all functions of the wide area of kwangyang Bay, the expansion of social indirect capital such as the de-velopment of road, harbor, airport should be given the precedence.

새마을工場의 實態分析과 生産性 向上方案 : With Focus on Saemaul Factories of Chunnam District.

金晋鎬,盧春燮,金明洙,李成春 순천대학 새마을연구소 1987 새마을硏究論文集 Vol.3 No.-

The purpose of this paper is to investigate the actual conditions of Sa―emaul Factories and then to suggest their productivity improvement program. The present research ceneteres around Saemaul Factories of Chunnam District, addressing the actual conditions of operation for better productivity. According to the above research, the following will be considered for pr―oductivity improvement program of Saemaul Factories. 1. The designation system of Saemaul Factories should be improved. 2. The impediment factors of operation should be eliminated for better productivity. 3. The educational program should be developed to strengthen members’ sense of productivity. 4. A more efficient assistance system should be sought on the part of government.

합성 Zeolite A 의 Detergent Builder 로써의 이용

김면섭,국명호 韓陽大學校 環境科學硏究所 1986 環境科學論文集 Vol.7 No.-

세제 builder로써의 합성 zeolite A를 하동 고령토로부터 합성하였다. 경도, 염화나트륨의 농도, linear alkyl benzene sulfonate와 합성 zeolite A와 sodium triphosphate의 비율을 달리 하는 세제 용액 중에서 non-finished cotton, resin finished cotton, polyester-cotton blend cloths를 세탁하여 세탁율과 칼슘이온농도를 측정하였다. zeolite A와 sodium triphosphate의 중량비가 1인의 경우의 실험결과는 다음과 같다. 세제의 양이 많을수록 세탁율은 증가하였으나 16˚d에서 3g/1 이상의 세제농도에서는 세탁율은 거의 증가하지 않았다. 세제농도는 경도 8˚d에서는 1.5g/1, 16˚d에서는 3g/1임을 알았으며 세탁율과 제거된 칼슘농도는 염화나트륨의 농도에는 무관하였음을 알았다. Synthetic zeolite A as a laundry derergent builder was prepared from domestic Hadong Kaolin. Non-Finished cotton, resin finished cotton and polyester-cotton blend cloths were washed in the detergent solutions which were containing various hardness, sodium chloride concentration and ratio of linear alkyl benzene sulfonate to zeolite A or to sodium triphosphate, and then cleaning performances and calcium ion concentrations were measured. The followings were the results of experiment in which the weight ratio of zeolite A to sodium triphosphate was 1. The more amount of detergent the more cleaning performance increased, but the performance was almost not inclreased with the concentration of detergent over 3g/1 at 16˚d. It was observed that the concentration of detergent was needed 1.5g/1 at hardness 8˚d and 3g/1 at hardness 16˚d. It was obtained that the cleaning performance and the calcium binding capacity were independent on the concentration of sodium chloride.

파스콘 및 콘크리트 트로프의 열방출 특성에 관한 연구

김현우,이창우,윤명오,이인섭,박인근 한국화재소방학회 2003 한국화재소방학회논문지 Vol.17 No.2

본 논문은 파스콘 또는 콘크리트 재질의 트로프에 설치되는 전선에 대한 열의 영향과 이들 트로프에 대한 열 방출 정도에 대한 차이점을 이론상으로 접근하고 평가하고자 하였으며, 실제 상황과 근접한 조건으로 컴퓨터 시뮬레이션을 실시함으로서 두 트로프 내의 전선에 대한 열의 영향과 두 트로프의 열 발산정도를 추정하고 그에 대한 타당성을 입증하고자 하였다. 이에 대한 연구 결과 파스콘의 고유 열전도 값과 콘크리트의 고유 열전도 값을 단순 비교하면 콘크리트 제품의 열 방출량이 우수한 것으로 판단할 수 있으나, 실제 설치되는 두 트로프의 실제 두께와 면적 및 세부 구조를 고려하여 연산 및 시뮬레이션을 실시해 본 결과 파스콘 트로프가 콘크리트 트로프 보다 다소 우위에 있는 것으로 평가되었다. Thermal effects and characteristics of heat release for electrical wire installed in pascon and concrete trough have been theoretically approached and studied in consideration of the structure of products. The points of difference between two troughs have been evaluated by theoretical approach and estimated by computer simulation in similar condition of institution environment. As a result, the average of the intrinsic thermal conductivity of concrete trough is superiority to pascon trough considering only for characteristic of materials. But when considering for institution environment(thickness, area and trough structure etc) as the results of the calculation and computer simulation using FLUENT, the degree of heat release for pascon trough is of advantage to concrete trough.

흰쥐 뇌세서의 Cholecystokinin mRNA 함유신경세포의 분포

김우태,배명애,이호섭,박매자,배용철,홍해숙,조희중,주강 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.4

목적 : 뇌에서 Cholecystokinin(CCK)-8 함유신경세포의 분포에 대해서는 면역조직화학적기법을 이용하여 행한 연구가 많으나 CCK mRNA함유신경세포의 분포에 대한 연구는 아직 단편적이므로 본 연구에서는 이들 신경세포의 분포를 정확히 밝히고져 하였다. 대상 및 방법 : 흰쥐 뇌에서 ^35S가 표지된 oligonucleotide를 이용한 in situ hybridization기법으로 관찰하였다. 결과 및 결론 : 종뇌; 후구의 전후핵, 신피질, 이상엽, 후내야, 외측, 기저 및 피질 편도핵, 해마체, 그리고 선조체에서 CCK mRNA함유신경세포가 관찰되었다. 간뇌; CCK mRNA함유신경세포가 시상의 경우 전내측시상핵, 전복측시상핵, 외측시상핵, 내측시상핵, nucleus reunions, nucleus rhomboideus, 그리고 내측슬상핵에서 관찰되었으며 시상하부의 경우는 시삭상핵, 실방핵, 등내측시상하부핵, 그리고 유두상핵에서 관찰되었다. 중뇌; 흑질의 밀집부, 복측피개야, nucleus linea rostralis, 중심회색질, Edinger-Westphal nucleus, 그리고 하구에서 CCK mRNA 함유신경세포가 관찰되었다. 교 및 연수; CCK mRNA 함유신경세포들이 lateral parabrachial nucleus 삼차신경척수로고유핵, 거대그물구성체핵, 연수그물구성체, 그리고 삼차신경척수로핵에서 관찰되었다. 이상의 결과를 지금까 보고된 CCK-8함유신경세포의 분포에 대한 면역조직화학적 연구결과와 비교 하였을 때 대부분의 부위에서 CCK-8 및 CCK mRNA 함유신경세포의 분포가 유사하였으나 시상의 경우 CCK-8 함유신경세포는 검출되지 않은 반면 매우 많은 수의 CCK mRNA 함유신경세포가 존재함을 알 수 있었고 대뇌 피질의 경우 CCK mRNA 함유신경세포수가 CCK-8 함유신경세포보다 월등히 더 많았다. 반면, 등측봉선핵 및 고속핵에서는 CCK-8 함유신경세포가 다수 존재함이 보고되어 있으나 본 연구의 경우 CCK mRNA 함유신경세포를 관찰할 수 없었다. In order to investigate the distribution of cholecystokinin(CCK) mRNA containing neurons in the rat brain, brains were extirpated rapidly, frozen sectioned and processed for in situ hybridization technique with ^35S labeled oligonucleotide probe. The results obtained were as follows Telencephalon; CCK mRNA containing neurons were found in the anterior olfactory nucleus, neo-cortex, piriform cortex, entsorhinal cortex, lateral, basal and cortical amygdaloid nucleus, hippocampus and corpus striatum. Diencephalon; In the anterior medial thalamic nucleus, anterior ventral thalamic nucleus, lateral thalamic nucleus, medial thalamic nucleus, nucleus reuniens, nucleus rhomboideus, and medial geniculate nucleus, CCK mRNA containing neurons were found. In the hypothalamus, CCK mRNA containing neurons were located in the supraoptic nucleus, paraventricular nucleus, dorsomedial nucleus and supramammillary nucleus. Midbrain; CCK mRNA containing neurons were found in the substantia nigra pars compacta, ventral tegmental area, nucleus linea rostralis, substantia gricea centralis, Edinger-Westphal nucleus and inferior colliculus. Pons and Medulla oblongata; CCK mRNA containing neurons were found in the lateral parabrachial nucleus, nueteus originalis nervi trigemini, nucleus reticularis gigantocellularis, nucleus reticularis medullae oblongatae, nucleus tractus spinalis nervi trigemini.

삼상 유동층에서의 기-액 물질전달특성

이명섭,김덕찬 서울市立大學校 1986 論文集 Vol.20 No.1

The characteristics of gas-liquid mass transfer in bubble column and three phase fluidized beds have been Studied in a 6.3cm I.D and 160cm height acryl column. The three phase fluidized beds were consisted of water, carbon dioxide mixed with oil-free compressed air and glass beads as liquid, gas and solid phases respectively. In this investigation, the effects of gas velocity (4.28-21.39cm/sec), liquid velocity (1.07-17.79 cm/sec) and particle size(1.3-6.0mm) on the ovlumetric mass transfer coefficient have been examined. The effects of the hight from the distributor on concentration profile and volumetric mass transfer coefficient were also determined. Volumetric mass transfer coefficient, was increased with increasing gas and liquid velocity, and particle sizes, but decreased with the height from the distributor. The concentration profile was showed exponential increasing with the height, but in the three phase fluidized beds, it was increased rapidly and decreased at once, i.e., it was exhibited the maximum value in the grid zone, and then increased continuously with the height. Volumetric mass transfer coefficient, K??, were correlated in terms of gas and liquid velocity and particle size; ????