김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

정신병적 증상이 동반된 기면병 1예

홍승철,송정민,정종현,권용실,한진희,이성필 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.5

Narcolepsy is a sleep disorder characterized by excessive daytime sleepiness, cataplexy, sleep paralysis, hypnagogic hallucinations and disrupted nocturnal sleep. We report a patient with a diagnosis of schizophrenia who was subsequently diagnosed as narcolepsy. A case of a 22-year-old woman with concomitant narcolepsy and psychotic symptoms was discussed from a perspective of the diagnosis and therapeutic challenges, particularly on pharmcological treatment dealing with issues raised by their coexistence.

과학과 수업 방법의 요인분석 연구

우종옥,정진우,홍성일 한국교원대학교 과학교육연구소 1995 청람과학교육연구논총 Vol.5 No.1

The purpose of this study was to find out and analyze the science teacher's teaching methods. A total of 35 teaching methods were abstracted from the previous studies and the relating literatures. An instrument to measure the frequencies of using methods was developed and then tested to middle school science teachers. The Results of two factor analysis methods were compared. The results are as follows: The instruments's reliablity coefficient(Cronbach α) was 0.7707. The teaching methods which middle school science teachers have used frequently were represented as the proposing of the learning objectives, the deductive teaching, the experimental activities by teacher's guide, the summarization after explanation, the reading text etc. Also, it was revealed that they have not use the diagnostic evaluation, the formative evaluation, the experimental activities by student's design, the instructional medium. By confirmatory factor analysis, the 1st factor included 13 teaching methods and 2nd and 3rd factor included 9 and 7 methods respectedly. The meaning of 1st factor was interpreted to stimulate student's learning motives. And the other's were about the development of instruction. In exploratory factor analysis factors were overlapped or more fined.These were due to the structure of factors.

생체용 Ti합금의 양극분극특성에 미치는 Zr 및 Pd의 영향

정종현,이길홍,최칠남,노학,백승남,이상열 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.2

The corrosion resistance of Ti-Zr-Pd-Cr alloys were investigated in order to reveal their possible use for new biomedical implant materials. Titanium alloys consisting of 10∼20%Zr, 0.0∼0.4%Pd and 0.15%Cr were prepared by vacuum arc-melting as alloying elements instead of using hardness Al and V in response to recent concerns about the long term safety of Ti-6%Al-4%V ELI alloys. The Ti-Zr-Pd-Cr alloys were heat-treated at 1000℃ for 2hrs under a vacuum atmosphere. The effect of Zr and Pd Ti alloys on the corrosion resistance for biomedical implant was investigating the anodic polarization curve at 37℃ in 5%HCI solution deaerated by high-purity N₂gas bubbling. The critical current density for passivation(I ) of Ti-Zr-Pd-Cr alloys didn't reveal markedly. Passive current density(I ) at 1.0V vs. SCE of this alloys decreased with increasing Zr in the contents from 10 to 20% and decreased with containing Pd in the range from 0.2 to 0.4%. Potential(E ) at 0.1A * m-² of this alloys increased with increasing Zr in the contents from 10 to 20% and increased with containing Pd in the range from 0.2 to 0.4%. For the Co-30%Cr-6%Mo alloy, passivity zone is narrow, and rapid increase of current density result from the low potential in comparison with Ti and Ti alloys. The corrosion resistance by the anodic polarization of Ti-20%Zr-0.2%Pd-0.15%Cr alloy was found to be superior to those of the Ti, Ti-6%Al-4%V alloy and Co-30%Cr-6%Mo alloy. The passive films formed on the Ti-20%Zr-0.2%Pd-0.15%Cr and Ti-6%Al-4%V alloys in the anodic polarization test consist mainly of TiO₂, Ti compound, ZrO₂, Al₂O₃and V₂O₃.

중공사 정밀여과 MBR공정을 이용한 제직폐수의 재이용

정용준,배종홍,민경석 한국물환경학회 2004 한국물환경학회지 Vol.20 No.4

Submerged membrane bio-reactor equipped with a hollow fiber microfiltration was applied to reuse weaving wastewater of water jet loom, where two parameters such as the concentration of MLSS and the flux were controlled. While the flux at the concentration of MLSS around 900㎎/L was constantly kept over 0.4m/d and 0.8m/d in a short time, the stable flux at around 300㎎/L of MLSS was shown at the 8 days later. Regardless of MLSS and flux, BOO, CODcr and Turbidity of the permeate were l~2㎎/L, 7~10㎎/L and below 1 NTU, which were 85~90%, 87~90% and 98% of removal efficiency, respectively. The stable operation without fouling was achieved because the contents of ECP were smaller than those of common MBR processes and the composition(saccharide/protein) was kept constantly. In this study, 0.5~1.0m/d of flux and 400~900㎎/L of MLSS were considered as the most recommendable operating condition for the reuse of weaving wastewater.

케이블 감속을 이용한 소형 로봇의 개발과 실시간 제어에 관한 연구

홍종성,이정완 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.B

In this thesis, a three degrees of freedom robot, which is able to provide sufficient precision for various robot researches, has been developed. The cable mechanism is used as a basic transmission of robot joints. Based on an optimal design strategy, link and joint parameters are determined and then overall geometry of the robot is designed. As an architecture of robot control, real time control system using real time linux and RtiC-Lab(Real Time Controls Laboratory) is developed. This system, written in C and based on Linux O/S, includes text editor, compiler, downloader, and real time plotter running in host computer for developing control purpose. Using these hardware and software, simple PD position control is implemented, the results shows the effectiveness of the system.

Monoclonal Antibody를 이용한 Streptococcus mutans 검출 방법의 임상적 적용에 관한 연구

홍희정,김종수,김용기 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

Monoclonal antibody를 이용한 Saliva-check Mutans키트의 타액 Streptococcus mutans검출방법으로서의 활용도와 임상적 우식지수 및 기존의 세균배양방법과의 상관관계를 알아보기 위해 2008년 2월에서 5월 중 평촌키즈웰치과에 내원한 만 2세에서 만 8세 사이의 92명의 아동을 대상으로 Streptococcus mutans 검출검사를 실시하였으며 또한 우식에 영향을 미치는 다른 요인인 치면세균막 pH와 타액완충능력검사를 실시하여 다음과 같은 결론을 얻었다. 1. Saliva-check Mutans 검사결과 양성을 나타낸 아동은 27명으로 29.3%이었고, 음성을 나타낸 아동은 65명으로 70.65%이었다. 우식경험유치면률은 음성 아동 13.89%, 양성 아동 25.23%으로 나타났다. 2. Monoclonal antibody를 이용한 검사방법 인 Saliva- Mutans와 기존의 세균 배양방법인 -SM은 각각 상이한 검사방법을 이용함에도 불구하고 검사결과 높은 상관관계를 보였다(p<0.01). 3. Saliva-check Mutans검사와 치면세균막 pH검사와는 역상관관계를 나타내었으나(p<0.01),타액완충능 검사와는 상관관계가 나타나지 않았다(p>0.05). 이상의 결과로 보았을 때 Monoclonal antibody를 이용한 검사방법인 Saliva-check Mutans는 구강 내 Streptococcus mutans를 측정하는 방법으로 적당하며 또한 검사에 필요한 시간을 대폭 줄일 수 있고 사용방법도 매우 간편하게 개발되어 환자들에게 적용함에 있어 효과적이라고 사료되었다. The purpose of this study was to evaluate the clinical effectiveness of new streptococcus detection system which used monoclonal antibody against Streptococcus mutans. 92 children aged between 2 and 8 were involved in this experiment and their saliva samples were collected for testing. Streptococcus mutans were measured by both monoclonal antibody-based detecting system (Saliva-check Mutans) and dip slide detecting system(Dentocult-SM). The results showed that Saliva-check Mutans levels had a significant correlation with dfs rate of subjects and the two test kits, Saliva-check Mutans and Dentocult-SM were shown to have a good correlation although they were based on different mechanism.

전치 태반의 임상적 고찰

홍관표,이성동,김왕수,정종일,장하종,정혁,한세준 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

This study was undertaken for the clinical evaluation and statistical analysis on the 38 cases of placenta previa who were admitted and treated from March 1, 1988 to Feb. 28, 1991. The results were as follows : 1. The incidence of placenta previa was one per 53 deliveries or 1.90% 2. There were 57.9 % total placenta previa, 21.1 % partial placenta previa, 13.1 % low-lying placenta previa and 7.9% marginal placenta previa. 3. Placenta previa has occured more often in multipara (63.2%) than primigravida 4. More than one-third (38.8%) cases of placenta previa had delayed admission until labor occured for some time following vaginal spotting has first time been noticed. More instructive prenatal education should be emphasized 5. Lower segment transverse section rate was 63.2%, classical cesarean section rate was 15.8% and cesarean hysterectomy was 9.8%. The rest of them were treated by vaginal deliveries of 5 cases(13.2%) 6. The perinatal loss associated with placenta previa was considered still higher : the premature death was 15.8% and term death was 2.6%. The problem of prevention of prematurity still remains to be studied. 7. There was no maternal mortality in our study

용량별 죽력 투여가 생쥐의 간과 신장의 조직 및 혈액학적 변화에 미치는 영향

정종길,나창수,김경수,홍광표,김정상 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objectives : Bambusae Caulis in Liquamen extracts (BE) has been used for various curative effect that remove intense heat from the heart, replenish deficiency Yin, nourish deficiency blood, resolve phlegm. This study was performed to investigate the toxicity of BE to mice according to the administered dosage. Methods : The experimental groups divided four. Control group treated saline, J1 group treated BE and distilled water in the ratio of 1:15(0.05ml/kg), F2 group treated BE and distilled water in the ratio of 1:7(0.05ml/kg), J3 group treated BE and distilled water in the ratio of 1:17(0.05ml/kg). We administered BE to the mice for 24 weeks at interval of 48 hours, and investigated the body weight, changes in hematological profile, activities of the transaminase(GOT, GPT) and two hepatic antioxidant enzyme(SOD, catalase), and histological changes of the liver and kidney. Results : Leukocytes of J3 group were increased in the administered for 12 weeks, but decreased in the administered for 24 weeks. Platelets were decreased about 36% in J3 group treated for 12 weeks, but increased about 50% in the same group treated for 24 weeks. Blood total proteins were decreased slightly in the all experimental groups compared with control group. The activities of the transaminase and superoxide dismutase(SOD) were increased, but decreased activities of catalase in the J2 and J3 groups administered for 12 or 24 weeks. In the light microscopical observation, ballooned and injured hepatocytes in portal vein area of J1 group occurred, and dilated hepatocytes and disoriented sinusoid observed in J2 group administered extracts to mice for 24 weeks. Apoptosis occured in the ballooned and injured hepatocytes of the portal vein area in J3 group. A agglutination of RBC in glomerular capsule of the kidney observed in the J2 group, and destructed convoluted tubule observed in the J3 group treated for 24 weeks. Conclusions : These results indicate that mice treated with high dose BE develop a hematological changes, dysfuncton of the liver and kidney.