Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

대학생의 전공에 따른 유행 동조성, 혁신성, 의복가치 차이에 관한 연구

장정임;윤지원;김수영;이규혜 한양대학교 2005 韓國 生活 科學 硏究 Vol.25 No.1

A change in economic and social environment has brought lots of changes in lifestyles and values of onsumers. This paper presents fashion conformity, innovativeness and clothing values of college students according to their academic majors. Respondents were 181 undergraduate students with diverse majors. The survey was conducted using a questionnaire designed to measure fashion conformity, innvativeness and utilitarian/hedonic clothing values. Seventeen items measuring fashion conformity and innovativeness and thirty six items measuring clothing values were adopted from existing studies. Chi-square, ANOVA, and multiple comparisons of means were conducted. Students were clustered into three groups of majors: Engineering/NaturalScience/Information majors (ENI), Business/Social/Humanity (BSH) majors and HumanEcology/Art/PhysicalEducation (HAP) majors. HAP majors were more innovative than ENI majors. Significant differences were found in hedonic and utilitarian aspects of clothing values. HAP majors had higher level of novelty/fun/attraction, fashion consciousness and social appraisal sensitivity dimensions. ENI majors showed higher level of comfort seeking among clothing values. No significance was found in clothing confidence, mix and match, economy, utility and handling dimensions of clothing values across different groups of majors. Results of this study imply that the consumer group according to a major has relation to fashion conformity, innovativeness and clothing values.

숙명박물관 브랜드 프로모션을 위한 통합적 브랜드 개발 -2D, 3D, 4D 디자인 프로세스를 중심으로-

길혜경,김보현,오정은,이서연,이지원,이진민,장미정,이성애 숙명여자대학교 산업디자인연구소 2017 숙명디자인학 연구 Vol.24 No.-

본 연구는 여성생활사 중심의 유물 박물관인 숙명박물관의 인지도와 브랜딩의 문제를 해결하기 위해 브랜드·스페이스·마케팅을 활용한 통합 브랜드 개발 프로세스를 제안하는데 목적이 있다. 아울러 본 연구는 박물관의 브랜드를 프로모션하고 그래픽, 제품, 공간의 통합 브랜드를 구축하는 일련의 프로세스를 밝히는데 목적이 있다. 이에 본 연구의 시간적 범위는 2017년 3월부터 6월까지 총 15주간 숙명여대 환경디자인학과 <브랜드·스페이스·마케팅>수업에서 진행된 수업의 결과물로서 4개 팀 중, 1개 팀의 작업물로 한다. 본 연구의 대상적 범위는 ‘숙명여자대학교 숙명박물관'의 2D~4D까지의 전반으로 설정한다. 그 결과, 제품부터 공간까지 숙명박물관의 통합된 브랜딩은 일 관성과 객관성, 스토리성을 얻을 수 있었다. ’숙명박물관'은 학교의 박물관이라는 장점을 극대화시켜 가치와 스토리를 부여하고 하나의 브랜드로 리뉴얼했다는 점에서 의의가 있다. 또한 향후 보편적인 박물관들의 차별화된 브랜드 개발의 참고자료로 활용될 수 있을 것으로 기대하며 브랜드 개발에 있어서 G·I, P·I, S·I의 통합 브랜드 메뉴의 기초 자료로 활용될 것으로 사료된다.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

Web에서 표현되는 아바타 디자인에 대한 시각적 표현 연구 : 사이버 커뮤니티 사이트를 중심으로

손지혜,장순석 동덕여자대학교 디자인연구소 2004 디자인포럼21 Vol.7 No.-

인터넷의 활성화와 멀티미디어의 발전은 새로운 커뮤니케이션 환경에서 가상공간이라는 다양한 분야를 생성시켰다. 인터넷 커뮤니케이션에서 익명성을 가지며 타인과의 상호작용을 형성해 주며, 이러한 가상공간의 활용으로 현대사회에서 이미 보편화된 하나의 문화로 인정 되고 있다. 가상문화에서 새로운 한계를 넘어 우리의 욕구를 충족시켜 줄 문화가 생겼는데 그것이 아바타이다. 과거에는 문자로써 나를 알리던 것이 이제는 아바타라는 그래픽으로써 표현되어지면서 네티즌들의 욕구표현이 증가 하고 아바타의 표현은 많은 발전을 가져왔다. 아바타를 이용하는 사용자들은 자신의 감정변화와 자신의 외모를 타인에게 알림으로써 나의 정체성을 확립시켜주고 자신의 존재를 가상세계의 표현수단으로 이용한다. 이제는 커뮤니티 아바타가 객관적인 자기표현에서 벗어나 내면적인 자아의 표현 특성을 시각화하는 아바타로 표현된다. 현대의 멀티미디어 사회에서 많은 대상들이 시각적 표현이 자극적으로 보이도록 유도되는 것이 인터넷 사회의 자연스러운 일이다. 단순하면서도 통일성을 가지며 시각적인 자극을 통해 아바타의 특성을 통하여 시각적인 표현을 한다. 본 논문은 아바타의 일반적인 정의를 기본으로 문화적인 의의 및 특징과 아바타와 캐릭터의 비교분석과 연계성을 알아보고 제 III장에서 아바타 디자인의 요소를 분석하고 아바타의 사례분석을 토대로 기존의 아바타의 시각적 표현의 문제점을 알아보고 아바타 디자인의 시각적 표현 개선방안과 아바타 디자인의 미래방향을 모색한다. Activation of the Internet and expansion of multi-media created various territory of cyber-space in new communication environment. Is having anonymousness in Internet communication and form interaction with another person, and is recognized to one culture that is already generalized on modern society by practical use of these cybe-rspace. Culture to satisfy our appetite passing over new boundary in imagination culture formed but it is avatar. As desire representation of netizens increases as become expressed as graphic that it is avatar now to inform me as character in the past, expression of avatar brought a lot of developments. Users who use avatar own emotion change and own appearance be indurated my identity as information to another person and uses own existence by expression means of the imagination world. Now, community avatar is expressed in avatar that visualize expression special quality of self who depart in objective self-expression and is the inside enemy. It is the Internet social natural matter that many targets are derived so that visual representation is seen saltily on modern multi-media society. Express thing which have unity and is optical through optical magnetic pole through special quality of avatar as is simple. This paper general narrow way of avatar searches cultural sense and characteristic and comparative analysis of avatar and Character and link sex to basis and analyzes constituent of avatar design in my III chapter and searches problem of visual expression of existent avatar on the basis of example analysis of avatar and gropes future direction of visual expression improvement plan and avatar design of avatar.

KS H ISO 1242 표준의 평가 : 라반딘 정유의 산값 측정

전혜림,이은지,장혜진,손현화,김건,김남선,전명숙,이동선 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-

The acid value (AV) as well as the ester value of essential oil is an important index to evaluate quality of essential oil. The official standard analytical method of KS H ISO 1242 dealing with “Essential oils - Determination of acid value” was assessed experimentally using two lavandin essential oils obtained in France as an example. One French lavandin oil sample showed the results of AV= 0.9 by both indicator method and potentiometry. Another sample showed AV = 1.9 by indicator method and AV = 1.6 by potentiometry. Latter lavandin oil sample exhibited significantly higher acid value than maximum value of 1 described in the standard. This result suggests that latter lavandin oil was contaminated, because this oil was contained in an orange juice bottle. Results of this study proved that KS H ISO 1242 could be used as a suitable scientific standard for the determination of acid value of essential oils.

서울 시내 4년제 남자 대학생의 우울과 흡연량, 흡연기간, 니코틴 의존도와의 관계

박지영,박소영,이미숙,백수진,신의경,예혜련,오승진,장윤정,조혜진,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

Background: With reports on the relation between smoking and depression appearing since 1980, it has become clear that depression is one of the psychodynamics of smoking, though there has been little or no progress made in the study of whether or not there is indeed a relation between depression and nicotine dependence. Purpose: The purpose of the study was to investigate the effects of depression on the amount and period of smoking and nicotine dependence among university man student smokers. Method: In periodical examination between 14 and 21, May, 2003, using 170 university male. The CES-D(Center for Epidemiologic Studies Depression) Scale and the FTQ(Fagerstrom Tolerance Qustionnaire) were utilized to measure the level of depression and nicotine dependency. The amount of smoking was calculated based on the number of cigarette used. Result: Results indicated that depression had something to do with amount of smoking. Nicotine dependence also was related to depression but total value was relatively low. finally the period of smoking had nothing to do with depression. Conclusion: The results of this study revealed that the amount of smoking of university man student smokers was very high. Both the amount of smoking and nicotine dependence were affected by levels of depression. Accordingly psychiatric nursing access such as emotional support is needed to manage depression and decrease smoking.

라우팅 브로커링에 기초한 분산 데이터베이스 액세스 메커니즘의 설계

신혜균,장지훈,김정선,최중민,우훈식 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

현재의 클라이언트 서버 환경에서는 수많은 이질적인 데이터베이스 서버들이 LAN과 WAN상에 분산되어 있다. 따라서 이들 서버의 위치에 관계없이 일관되고 확장성 있는 액세스 메커니즘을 클라이언트에게 제공하는 것이 필요하다. 본 논문에서는 라우팅 브로커링에 기초하여 분산되고 이질적인 데이터베이스에 대한 일관되고 위치 투명성과 확장성 있는 액세스 매커니즘을 제시한다. 본 고에서 제안한 메커니즘은 클라이언트와 다수의 서버사이의 중간계층으로서 작동되도록 java와 CORBA 그리고 JDBC의 최신 기술을 적용시켜 설계되었다. In current client/server environments, it is not uncommon to have multiple, heterogeneous database servers scattered on LAN or WAN. Therefore, it is desirable to provide clients with uniform and scalable access mechanism to connect to those servers in a location-transparent way. In this paper, we propose a uniform, location-transparent, and scalable access mechanism based on routing brokering scheme to access distributed, heterogeneous databases on LAN. The proposed mechanism is designed to act as a middle tier between clients and multiple servers, and adopts Java, CORBA and JDBC as its state -of-the -art techniques.

제7차 「기술 · 가정」 교과에 대한 학생의 인식

최지혜,장현숙 경북대학교 사범대학 부속 중등교육연구소 2005 중등교육연구 Vol.53 No.2

본 연구는 2001학년도부터 시행되어 온 제7차 교육과정에서 「기술 · 가정」 교과에 대한 학생의 인식을 알아보는 데 목적이 있으며, 이를 위해 2004학년도 12월 현재 대구광역시에 소재한 7학년부터 10학년까지의 학생 1065명을 조사한 결과 다음과 같이 결론을 내릴 수 있다. 첫째, 「기술 · 가정」 교과에 대한 학생의 인식에서 성별에 따라서는 여학생이 남학생보다 「기술 · 가정」 교과를 더 실용적으로 인식하고 있었으며, 학년에 따라서는 9학년 학생이 다른 학년의 학생보다 「기술 · 가정」 교과에 대한 흥미도, 중요도, 실용도가 낮게 나타났고, 운영형태에 따라서는 7학년 학생이 전담지도보다는 분담지도의 경우 더 흥미있어하고 덜 어려워하는 것으로 나타났다. 둘째, 학생의 흥미도, 중요도, 실용도, 난이도의 상관관계를 살펴보면, 실용적인 것을 중요하게 여기고, 흥미가 있으면 중요하게 여기고 중요한 것에 흥미를 나타내며, 실용적인 것에 흥미를 나타내는 것으로 나타났으며, 어려울수록 흥미를 잃는 것으로 나타났다. The purpose of this research was to find the perception of students on the subject 「technology and home economics」 in the 7th national curriculum. For this research, questionnaires were sent by post to middle school students and first graders in high school. Then they were collected. The number of students' questionnaires was 1082 and 1065 questionnaires were used for being analyzed. The results of this research were summarized as follows. 1) Students' perception about the subject, technology and home economics, was affected by sex, grade, and implementation types. Girls' perception on the subject was more practical than boys'. Students in the 9th grade showed lower interest, importance, practicality on the subject than those in the other grades. According to implementation types, students in the 7th grade showed more interests in the partial charge than in the whole charge. 2) Considering correlation among interest, importance, practicality and difficulty, they thought of practical things as important and when they were interested in some things, they thought them important. They got interested in what they thought important and practical. And the more difficult the subject was, the less interested they were in it.

역류식 충진탑에서 수력학적인 분석과 이산화탄소의 탈착에 관한 연구

楊善惠,崔東勳,지혜미,金恩貞,金彰昊 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.1

This study was carried out to interpret hydraulic behavior and CO₂gas desorption in countercurrent packing tower which packed 50 mm plastic Hiflow-ring. The results are as follows: 1. To compare with conventional packing, 50mm Hiflow-ring could save energy because of low pressure drop under high load. 2. As relative error between calculated value and investigated value was less than 6% in the loading point and flooding point we found that we can predict results mathematically which occurres in the packing tower. 3. The unique magnituteds of packing which was used are as follows. C_(L) = 2.1×10^(-4), n = 0.787 so we can predict efficency which occurres.