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      • KCI등재

        제타전위를 이용한 키토산의 응집효과

        이동용,박상순,정병옥,정석진 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.1

        By adjusting the reaction conditions of crab shell, chitosan CsA, CsB and CsC were prepared, respectively. The deacetylationdegree of prepared CsA, CsB, CsC was 91.3%, 94.6% , 97.1 %, and the viscosity average molecular weight were 9.8×105, 6.9×105, 4.2×105, respectively. The particle size was increased with the molecular weight of chitosan increasing. and Zeta potentialwas also decreased with the molecular weight of chitosan increasing. The trend of Zeta potential showed parabola-shaped curvewith increasing dose. The optimal dosage of zeta potential was 1.2 mL for 5000 ppm, 1.5 mL for 3000 ppm, and 1.8 mL for1000 ppm, respectively. The trend of Zeta potential showed mountain-shaped curve with increasing dose. The dosage of largeparticle size form was 1.2 mL for 5000 ppm, 1.5 mL for 3000 ppm, and 1.8 mL for 1000 ppm respectively. At the same dosageof chitosan, the higher the molecular weight and concentration were excellent. In order to increase the utilization of the wastewater treatment, chitosan should be applied by both of molecular weight and dosage for concentration of chitosan. 게 껍질을 반응조건을 조절하여 키토산 CsA, CsB와 CsC 를 제조하였고 얻어진 결과는 다음과 같다. 제조한 CsA, CsB, CsC의 탈아세틸화도는 91.3%, 94.6%, 97.1% 이었으며 점성도평균분자량은 9.8×10 5 , 6.9×10 5 , 4.2×10 5이었다. 분자량이클수록 입자크기는 증가하였다. 또한 분자량이 클수록 제타전이가 낮았다. 투입량이 증가할수록 제타전이는 감소하다가증가하였다. 최적의 제타전위를 보인 투입량은 5000 ppm에서 1.2 mL, 3000 ppm에서 1.5 mL, 1000 ppm에서 1.8 mL 이었다. 투입량이 증가할수록 입자크기는 증가하다가 감소하였다. 가장 큰 입자크기를 보인 투입량은 5000 ppm에서 1.2 mL, 3000 ppm에서 1.5 mL, 1000 ppm에서 1.8 mL이었다. 키토산용액은 동일한 투입량에서는 분자량 및 농도가 높을수록 우수하였다. 키토산을 폐수처리에 활용도를 높이기 위해서는, 키토산농도에 대한 분자량 및 투입량을 선별적으로 적용해야 됨을 알았다.

      • KCI등재

        제 2 회 한국 키틴 , 키토산연구회 심포지움 Proceeding : 키틴 및 키토산을 이용한 의료용과 분리막으로의 응용 - 2 키토산계 분리막을 이용한 유기혼합물의 투과증발분리 -

        이영무(Young Moo Lee),남상용(Sang Yong Nam) 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.3

        Chitosan membranes were prepared by solution casting method. A series of chemically modified chitosan membranes containing carboxymethy1, carboxyethy1, cyanoethy1, amidoxime, sulfony1 and phosphory1 groups has been preapred for separating ethanol-water mixtures in pervaporation. Polyelectrolyte complex membranes were prepared from chitosan and poly(acrylic acid) by mixing two polymer solutions and tested pervaporation for the separation of water/ethanol, water/isopropanol and methano/methy1 t-buty1 ether mixtures. Chitosan composite membranes were also prepared for the enhancement of permeation flux and then modified by surface crosslinking and complexation with anionic surfactants. Surface crosslinked chitosan composite membranes showed good pervaporation performance for the separation of aqueous solutions of ethanol, isopropanol and ethylene glycol. The b-chitosan composite membrane was prepared for the pervaporation of water/alcohol mixtures. b-Chitosan composite membrane showed a better pervaporation performance than that of a-chitosan membrane, particularly a better permeation flux. Surfactant complexed chitosan composite membranes also showed good pervaporation performance for the separation of methanol/methy1 t-buty1 mixtures.

      • KCI등재
      • KCI등재

        제 3 회 한국키틴키토산연구회 심포지움 Proceeding : 저농도 산분해방법에 의한 기능성 키틴 , 키토산 가수분해물의 제조 및 이용

        이정석(Jung Suck Lee),주동식(Dong Sik Joo),조순영(Soon Yeong Cho),조만기(Man Gi Cho),이응호(Eung Ho Lee) 한국키틴키토산학회 1998 한국키틴키토산학회지 Vol.3 No.1

        Chitin and chitosan were hydrolyzed by microwaving and autoclaving treatment in a low concentration of hydrochloric and organic acid in order to develop new hydrolysis methods under low pollution conditions. For high yield of chitin hydrolysates prepared by physicochemical method in a low concentration of HCI, a microwaving treatment was more effective than autoclaving treatment. The molecular weight of chitin was lower, the yield of chitin hydrolysates was higher. Through a physicochemical method using a 0.575 organic acid, a higher yield of chitosan hydrolysates was reached through a microwaving treatment than autoclaving treatment. The molecular weight of chitosan was lower, the yield of chitosan hydrolysates was better. Among the organic acids, 0.5% succinic acid was the best solution in physicochemical hydrolysis of chitosan. Chitosan hydrosates were higher antimicrobial activity than chitin hydrolysates. In minimum inhibitory concentration on the growth of bacteria, chitosan hydrolysate was lower than its oligosaccharides fraction(MW< 10,000). All the hydrolysates of chitin and chitosan had anticavitied activity, not the ACE inhibitory activity. Chitosan hydrolysate prepared by microwaving treatment in 0.5% succinic acid for 200min at 121 ℃ was added into fish paste meat, then the change of quality of fish paste meat was measured during storage at 5℃. Until 18th day in storage, viable cell count was lower than the control, jelly strength and hardness was high.

      • KCI등재

        추출조건에 따른 오만둥이 유래 다당류의 이화학적 특성 및 항산화 활성

        이대훈 ( Dae Hoon Lee ),홍주헌 ( Joo Heon Hong ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.2

        In this study, we extracted polysaccharides from Styela plicata, and investigated to determine its physicochemical properties and antioxidant activities. The yields of hot water extract (SHE), autoclave extract (SAE), and enzyme extract (SEE) were 8.6, 10.4, and 11.9%, respectively. The uronic acid contents were 10.86 g/100 g (SEE), 6.13 g/100 g (SAE) and 3.12 g/100 g (SHE), respectively. The hexosamine and sulfate-glycosaminoglycans contents of SEE was highest of 21.40 g/100 g, 33.78 g/100 g respectively. Composition of amino acids was mainly leucine, valine, serine and threonine. With regard to mineral content, P was the highest in all samples, followed by Ca>Mg>K, and Fe contents. The spectrum of FT-IR showed wavenumber at 3,300~3,400 cm-1, 2,900 cm-1, 1,650 cm-1, and 820~850 cm-1 representing the polysaccharide. The decomposition temperature was in the range of 300 and showed relatively good enough for their thermal stabilities. The oxygen radical absorbance capacity (ORAC), FRAP activity and ferrous ion chelating effect of SEE were 215.38 μmole TE/g, 295.38 μM, and 27.79%, respectively. Electron donating abilities, reducing power and tyrosinase inhibition activity increased with an increase in extract concentration.

      • KCI등재

        산수유 꽃 추출물의 항산화 활성에 키토산이 미치는 영향

        유보람 ( Bo Ram Yoo ),김진미 ( Jin Mi Kim ),최지선 ( Ji Sun Choi ),정병옥 ( Byung Ok Jung ),정석진 ( Suk Jin Chung ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.2

        The purpose of this study was to investigate the antioxidant activities of the D.W., EtOH, MeOH, BuOH, and EtOAc extracts of Cornus officinalis flowers (COF), and COF extracts+Chitosan (Cs). As a result, the yield of extract showed the highest value in an D.W. extract. The total polyphenol, flavonoid, and tannin showed 98.95 mg GAE/g, 17.96 mg QE/g, and 81.26 mg TAE/g at the EtOAc extract. The COF extracts at a concentration of 1 mg/mL, the ABTS radical scavenging activity and FRAP assay indicated the hightest activity with EtOAc extract. And DPPH was high value in an EtOH extract (90.57%). We knew that the EtOAc extract is good antioxidant potential. It can be seen that these effects are due to the polyphenol and flavonoid. The addition of Cs in the extracts of COF improved the DPPH and ABTS radical scavenging activity. As the result, extracts of COF and Cs are going to be used as a nature antioxidant and antioxidants substance for functional foods.

      • KCI등재

        Antibacterial Activity of Chitosan against Antibiotics Resistance Bacteria in Porcine Semen

        ( Da Gyung Lee ),( Dae Young Kim ),( Jae Kweon Park ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.3

        Bacteria in porcine semen having resistance on antibiotics decrease the quality and viability of sperms. In the continuing search for potential antibacterial of chitosan with different molecular weight, we have isolated bacterial strain from porcine semen and applied chitosan to address their antibacterial activity. However, definitive evidence for this relationship between different molecular weight chitosan and its antibacterial activity toward some bacterial strains in porcine semen was in lacking. The results showed that no significant synergic effects of chitosan with different molecular weight with antibiotics on bacteria isolated from two different sources of semen were observed. However all chitosan and glucosamine used in this study showed antibacterial activity toward ARB, which showed the resistance especially toward ampicillin and erythromycin. Our data suggest that chitosan can be used as potential therapeutic agents in the treatment of porcine semen that could specifically target antibiotics resistance bacterial cells (ARB).

      • KCI등재

        제타전위를 이용한 키토산의 응집효과

        이동용 ( Dong Yong Lee ),박상순 ( Sang Soon Park ),정병옥 ( Byung Ok Jung ),정석진 ( Suk Jin Chung ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.2

        By adjusting the reaction conditions of crab shell, chitosan CsA, CsB and CsC were prepared, respectively. The deacetylation degree of prepared CsA, CsB, CsC was 91.3%, 94.6% , 97.1 %, and the viscosity average molecular weight were 9.8×10 5 , 6.9× 10 5 , 4.2×10 5 , respectively. The particle size was increased with the molecular weight of chitosan increasing. and Zeta potential was also decreased with the molecular weight of chitosan increasing. The trend of Zeta potential showed parabola-shaped curve with increasing dose. The optimal dosage of zeta potential was 1.2 mL for 5000 ppm, 1.5 mL for 3000 ppm, and 1.8 mL for 1000 ppm, respectively. The trend of Zeta potential showed mountain-shaped curve with increasing dose. The dosage of large particle size form was 1.2 mL for 5000 ppm, 1.5 mL for 3000 ppm, and 1.8 mL for 1000 ppm respectively. At the same dosage of chitosan, the higher the molecular weight and concentration were excellent. In order to increase the utilization of the waste water treatment, chitosan should be applied by both of molecular weight and dosage for concentration of chitosan.

      • KCI등재

        Growth and Quality of Soybean Sprouts Affected by Chitosan Treatment and Watering Time

        ( Yo Sep Kim ),( Na Young Park ),( Hong Kyoon No ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.3

        The effects of chitosan concentrations (0.05 and 0.1%) and watering times (1 and 5 min/every 20 min) on growth and selected quality of soybean sprouts were evaluated. In chitosan concentration studies, soaking of soybeans in 0.05% chitosan solution was more effective in increasing total weight of soybean sprouts than soaking in 0.1% chitosan solution. In chitosan treatment and watering time studies, soybeans were soaked in water (W) or 0.05% chitosan solution (CH) for 8 h and then cultivated by spraying water for 1 min (W-1 and CH-1) or 5 min (W-5 and CH-5) for 3 days at 20±2oC. Increase in watering time from 1 min to 5 min increased total weight of soybean sprouts (W-1 vs. W-5 and CH-1 vs. CH-5). At the same watering time, chitosan treatment was more effective in increasing total weight than water treatment (W-1 vs. CH-1 and W-5 vs. CH-5). No significant differences in hardness, mineral content, and DPPH radical scavenging activity were observed among four treatment groups. However, the moisture content increased with increasing watering time. Total free amino acid contents of W-5, CH-1, and CH-5soybean sprouts were all comparable, but higher than that of W-1 soybean sprouts. Increased shelf life of CH-5 soybean sprouts, compared with W-5, was not observed during storage at 10°C for 21 days.

      • KCI등재

        Anti-inflammatory Potential of Chitin, Chitosan and Chitooligosaccharides: A Review

        ( Gaurav Lodhi ),( Yon Suk Kim ),( Jin Woo Hwang ),( Se Kwon Kim ),( You Jin Jeon ),( Sang Ho Moon ),( Byong Tae Jeon ),( Pyo Jam Park ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.3

        Inflammation is primarily a defense mechanism of the host against invading pathogens with the main purpose being mainte-nance of tissue homeostasis. However, if it goes uncontrolled or unchecked, it leads to a plethora of pathological conditions including tissue damage, tumorogenesis and even organ failure. Chitin is a non-toxic, natural biopolymer synthesized in enor-mous amount by living organism. Chitosan is a partially deacetylated derivative of chitin. Both chitin and chitosan have been in the center of research because of their functional properties but poor solubility limits their application. Chitooligosaccharides(COS) are chemically or enzymatically degraded products of chitosan or chitin with an advantage of increased solubility. The application of chitin, chitosan and COS varies from being used in food, cosmetics, biomedicine, agriculture, environmental protection, and waste water management to being used as a bioactive material and carriers in drug delivery. In light of the various proven biomedical applications of chitin, chitosan and COS, this review focuses on their anti-inflammatory potential.

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