Purification and Characterization of Thermostable β-Glucosidase from the Brown-Rot Basidiomycete Fomitopsis palustris Grown on Microcrystalline Cellulose

Jeong-Jun Yoon,Ki-Yeon Kim,Chang-Jun Cha 한국미생물학회 2008 The journal of microbiology Vol.46 No.1

An extracellular β-glucosidase was purified 154-fold to electrophoretic homogeneity from the brown-rot basidiomycete Fomitopsis palustris grown on 2.0% microcrystalline cellulose. SDS-polyacrylamide gel electrophoresis gel gave a single protein band and the molecular mass of purified enzyme was estimated to be approximately 138 kDa. The amino acid sequences of the proteolytic fragments determined by nano-LC- MS/MS suggested that the protein has high homology with fungal β-glucosidases that belong to glycosyl hydrolase family 3. The Kms for p-nitorophenyl-β-D-glucoside (p-NPG) and cellobiose hydrolyses were 0.117 and 4.81 mM, and the Kcat values were 721 and 101.8 per sec, respectively. The enzyme was competitively inhibited by both glucose (Ki= 0.35 mM) and gluconolactone (Ki= 0.008 mM), when p-NPG was used as substrate. The optimal activity of the purified β-glucosidase was observed at pH 4.5 and 70°C. The F. palustris protein exhibited half-lives of 97 h at 55°C and 15 h at 65°C, indicating some degree of thermostability. The enzyme has high activity against p-NPG and cellobiose but has very little or no activity against p-nitrophenyl-β-lactoside, p-nitrophenyl-β-xyloside, p-nitrophenyl-α-arabinofuranoside, xylan, and carboxymethyl cellulose. Thus, our results revealed that the β-glucosidase from F. palustris can be classified as an aryl- β-glucosidase with cellobiase activity.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

유아교육실습 지도교사 교육프로그램 개발 연구

윤기영,정현숙,류칠선 서원대학교 교육연구소 2001 敎育發展 Vol.20 No.2

The purpose of this study was to develop a in-service teacher education program for student teaching in early childhood education center in order to reinforce student teacher's educational role for improving their student teaching. In this paper, the sample consisted of 20 early childhood professionals who have experienced at least 3 years in students teaching program. The survey was administered using the Delphi method. The study explored the objectives, contents, administration and method, and evaluation of the developed program. We also attempt to outline the present conditions and problems on student teaching in early childhood education center. As a conclusion, the program have to assist the able kindergarten teacher for understanding student teacher correctly, to consist and administrate a practicum program with systematic support.

Carbamoylethyl Starch의 製造와 종이 製造時 添加效果에 관한 硏究

尹世永,崔正箕,鄭文箕,趙炳默,吳正壽 동국대학교 생명자원과학대학 연습림 1995 연습림논문집 Vol.- No.4

Recently, paper strength properties come up to be lowered by increasing ratio of utilization of recycling paper. In order to overcome these problems, strengthening agents which may cause enviromental problems have been used. Therefore, acrylamide was added to starch which is a cheap natural polymer, carbamoylethyl starch(CES) with high activity was prepared, and so the applicability as a paper additive was examined. The results are following; FT-IR spectra of CES of which degree of substitution was 0.25 showed strong C=O band at 1680㎝^-1 which corresponds the carbonyl group n amides(amide Ⅰ). Analysis of bravender viscosity showed that cooking start point of CES is 60℃ and instant viscosity is 830 B.U.(bravender unit). Effect of carbamoylethl DS on strength properties was resulted that CES of DS 0.20 appeared to be the best choice for making handsheets from KOCC with good overall strength properties, and it was selected as standard sample. Effect of pH on strength properties was obvious for their increasing values as decreasing pH. Also, a 5-fold increase in size rate as well as significant increase in wet-tensile strength were obtained in handsheets from KOCC by 3% wet-end addition of CES.

敎育改革과 忠北敎育發展

尹基永,鄭賢淑 청주대학교 새마을연구소 1988 새마을硏究論文集 Vol.5 No.-

It is the purpose of this study to analysis of educational character, structure, and problems was related to 「Education Reform」 in Chung-buk for developing plan. The results of this study are as follows 1. Aspect of quantity, the education of Chung-buk was developed quickly through past 40years. But they have quality problems for developing more effective education. 2. Other characters of education in Chung-buk are complexity structure and many difference between each city or country in quality. So planning for city and country type educational model is needed. 3. The most problem is poor budget for education. change the tax system, contribution plan, issuance of public deventure are effective strategy to improve the educational structure in Chung-buk.

악관절내장증을 동반한 Eagle's Syndrome

윤중호,김기정,정상훈,박광호,박정현,김형곤 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.4

A thirty two year-old male was referred with a chief complaint of mouth opening limitation, and maxillofacial pain including left TMJ area. The patient had been treated in a private clinic with medications and conservative treatments, without any improvements in symptoms. MRI findings showed a limitations in condylar head movements, and signs of disc adhesion without the Positional change. Panoramic views showed elongation of stylohyoid process. Brain C-T was taken due to the patient's complaint of headache, facial pain, and paresthesia of tongue, and the result showed no abnormalities. The first surgery included meniscoplasty and the removal of disc adhesion of left TMJ under the preliminary diagnosis of internal derangement with adhesion, and the patient displayed marked improvements in opening movements despite of persistent hemiparesthesia of left facial areas and tongue, and mild dysphasia. Therefore, the second surgery was carried out with the preliminary diagnosis of Eagle's Syndrome, and a resolution of neurologic symptoms was obtained. The final diagnosis of this case was Eagle's Syndrome with internal derangement, and the difficulties in diagnosis and treatment might be due to the existence of two separate diseases with similar symptoms.

SWS 490B 강의 피로균열 진전시 음향방출 거동과 파단면 관계

鄭中采,尹東珍,朴徽立,金基福,李承錫 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.8

The objective of this study was to investigate the effect of AE activities during fatigue test. Laboratory experiment was carried out to identify AE characteristics of fatigue cracks for compact tension specimen. The relationship between a stress intensity factor and AE signals activity was discussed. From the experimental results, the features of specific parameters such as the length of crack growth, the AE energy and the AE peak amplitude, showed similar trend in their increase as the stress intensity factor increased. The fracture surface of test specimens revealed both intergranular fracture and transgranular fracture. Size and density of slip band were varied as the stress intensity factor increased and AE activities were different according to the slip band shape.

주성분 회귀분석 및 인공신경망을 이용한 AE 변수와 응력확대계수와의 상관관계 해석

윤동진,김기복,정중채,박휘립,이승석 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.1

AE 신호와 재료의 기계적 물성과의 관계를 정량적으로 제시할 수 있는 방법을 개발하였다. 재료의 여러 가지 기계적 성질들 중 피로균열 거동에 관련된 응력확대계수를 중심으로 AE 신호와 같은 다변량 데이터의 처리에 많이 사용되고 있는 주성분 회귀분석과 비선형적 문제 해결에 적합한 신경회로망 기법을 이용하였다. 이를 위하여 강교량 부재인 SWS490B 강에 대한 피로균열전파 실험을 수행하였으며 표준 CT 시편에 대한 피로균열진전 시 발생하는 AE 신호의 각 변수와 응력확대계수와의 관계를 고찰하였다. 통계분석 방법인 변수선택법을 적용한 결과 AE 카운트(RC), 에너지(EN), 신호지속시간(ED)의 각각에 대한 유의성이 높은 것으로 나타났으나 전반적으로 전체 AE 변수를 모두 이용할 경우 통계적 유의성이 높은 것으로 나타났다. 부재의 반복하중 시 발생하는 피로균열진전을 정량적으로 도출할 수 있는 응력확대계수 추정모델을 개발하고 평가하였다. 미지 시료에 대하여 개발된 모델의 응력확대계수 예측 성능을 분석한 결과 주성분 회귀모델과 인공신경망 모델 모두 우수한 예측성능을 나타내었으나 전반적으로 인공신경망 모델이 주성분 회귀모델보다 다소 양호한 것으로 분석되었 다. The aim of this study is to develop the methodology which enables to identify the mechanical properties of element such as stress intensity factor by using the AE parameters. Considering the multivariate and nonlinear properties of AE parameters such as ringdown count, rise time, energy, event duration and peak amplitude from fatigue cracks of machine element, the principal component regression(PCR) and artificial neural network(ANN) models for the estimation of stress intensity factor were developed and validated. The AE parameters were found to be very significant to estimate the stress intensity factor. Since the statistical values including correlation coefficients, standard error of calibration, standard error of prediction and bias were stable, the PCR and ANN models for stress intensity factor were very robust. The performance of ANN model for unknown data of stress intensity factor was getter than that of PCR model.

유아교육교사와 유아의 인간관계

윤기영,지옥정 서원대학교 교육연구소 1988 敎育發展 Vol.7 No.1

The purpose of this study is to provide desirable directions for human relations in Early childhood education. To serve this purpose, the meaning, characters and ideal teacher's role are observed. 1.The characters of human relations between kindergarten teachers and young children are ① Teaching and learning relations, ② Hardly have choice relations, ③ Giving and Taking influences relations, ④ Sharing love relations, ⑤ Complicated relations. 2.The idel teacher's role and attitudes are ① qualified teachers, ② Authoritative teachers, ③ Sharing love effectively, ④ Trying to develop themselves, ⑤ Growing with young children. 3.As conclusion, It is necessary to establish more effective ability of human relations for Kindergarten teachers. It is recommended to have lecture of human relations in teacher's college.

양측 하악과두 골절에 대한 관혈적 정보술후 나타난 근약화와 시력장애 : 증례보고

정기훈,신용길,김태열,신재명,윤규호,전인성 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.6

The condylar fracture show high occurrence rate in the following of symphysis and angle, because it is easy to have concentration of outer pressure due to its long and narrow anatomical shape and mouth opened state when trauma is attacked. For the clinical findings, there are trismus, opening deviation, mal-occlusion and open bite The classification of condylar fracture is varied due to the location of condylar bone fragment, fracture site. There are conservative and surgical methods for treatment modalities. Because of many advantages, conservative method is widely used it has no particular limits of treatment adjustment but the cases of absolutely surgical correction indication are easily found in clinical experience. Although many methods are suggested for treatments of condylar fracture, there are no particular surgical methods for precise reduction due to difficult accessibility in high condylar neck fracture. For precise reduction of condyle, we used Nam's method. However, in Nam's method, because condyle is fixed when once it is kept off externally reduced in avascular state, so complication such as condyle resorption is pointed out as a problem. In our clinic, we obtained some knowledge by treating a patient who had muscle weakness and vision disorder after temporary airway obstruction follownig the both condylar fracure operated using Nam's method, so here we reported the case with a study of documents.