Bacillus subtilis SS103으로 제조한 된장의 품질 특성 및 Angiotensin 전환효소 저해 활성

홍지수,박정륭,전정례,차명화,김진,김정희 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.4

Three different doengjang(fermented soybean paste) were prepared by using B. subtilis SS103 with high angiotensin converting enzyme(ACE) inhibitory activity, Asp. oryzae and mixed culture of two organisms, respectively. Quality characteristics and the inhibitory activity were compared. β-Amylase activity rapidly increased during 30 days of fermentation, but lower activity was found in doenjang prepared with B. subtilis. However, α-Galactosidase activity decreased for 15 days of fermentation and little activity was observed after that fermentation period. Protease production tended to increase during the fermentation. High protease activity was found only in doengjang prepared with Asp. oryzae, but the doengjang prepared with B. subtilis SS103 showed very low activity. Total isoflavone content at 90 day fermentation was the highest in doengjang prepared with mixture of Asp. oryzae and B. subtilis. ACE inhibitory activity increased during the fermentation period. The highest activity was found in doengjang made with mixture of Asp. oryzae and B. subtilis. Sensory evaluation on doengjang itself and its soup indicated that doengjang made with mixture of Asp. oryzae and B. subtilis showed higher acceptability on taste, flavor and color than those prepared with only Asp. oryzae or B. subtilis, respectively.

혼합형 자가면역성용혈성빈혈과 동반된 Evans 증후군 1례

선길홍,윤찬영,박상곤,박경희,우정주,한경택,김진화,김영훈,정춘해,박치영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Evans syndrome is defined as a simultaneous or sequential occurrence of autoimmune hemolytic anemia and autoimmune thrombocytopenia. Autoimmune hemolytic anemia (AIHA) is usually induced by IgG warm autoantibody or cold-active IgM antibodies reacting specifically with antigens associated with a patient's RBC. AIHA is a fairly uncommon disorder, with estimates of the incidence at 1 to 3 cases per 100,000 per year. Mixed-type AIHA is a relatively uncommon form of AIHA, with studies noting the incidence of 7-8% among cases of AIHA. We experienced a patient, 46-year-old woman who was diagnosed having a very rare clinical presentation of mixed warm and cold antibody mediated Evans syndrome. She was treated with corticosteroid therapy only and has been maintaining a complete response for 15 weeks. 저자들은 혼합형의 자가면역성 용혈성 빈혈과 자가면역성 혈소판 감소증이 동시에 발생한 혼합형 Evans 증후군으로 진단하고 스테로이드 요법 후 혈액학적으로 회복된 상태로 15주가 지난 현재 steroid 5 mg/일 까지 감량한 상태에서 추적 관찰 치료중인 46세 여자 환자를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

장기간 보관한 멸균물품의 오염 상태에 관한 연구

주홍순,조순덕,정재심,신동분,임바올라,노은지,이선희,송영미,최정화,백병윤,김미나 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.1

결핵군 55 kDa 항원의 면역학적 분석

김화중,김홍성,임재현,조은경,송창화,박정규 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Identification and characterization of mycobacterial antigens are critical for evaluation of their role in diagnosis, vaccination, and pathogenesis of mycobacterial diseases. An attempt has been made to immunologic characterize the 55 kDa protein antigen of Mycobacterium tuberculosis H37Rv which attracted our interest because it is present in high concentration in 50-80% ammonium sulfate fraction of the culture filtrate. Two monoclonal antibodies (MAbs) directed to 55 kDa antigen were produced. MAbs MT55-1 and MT55-2 reacted with a single 55 kDa protein band. On examination of degree for cross-reactivity with other mycobacterial species by enzymelinked immunosorbent assay (ELISA) and immunoblotting, these antibodies reacted strongly with M. tuberculosis and M. bovis BCG, and reacted weakly with M. marinum and M. smegmatis. To investigated the subcellular distribution of MAbs defined epitopes in the 55 kDa antigen within the mycobacterium, we isolated three major subcellular factions of M. tuberculosis, namely, cell wall, cytoplasmic membrane, and cytosol, by a simple fractionation procedure. MAb MT55-1 reactive cpitope was found in the cytosol when tested by immunoblotting. A sandwich ELISA was initially developed for detecting 55 kDa antigen using MT55-1 MAb in mycobacterial culture filtrate before detecting it in clincal specimens. The minimal detectable concentration was 1.0 ㎍/m1 for M. tuberculosis culture filtrate and 100 ㎍/ml for sonic extracts of M. bovis BCG and M. marinum, respectively. But the 55 kDa antigen was not detected in sonic extracts of other mycobacterial species examined. Although further evaluations are required, this study suggests that the 55 kDa antigen may be of interest as potential diagnostic reagent.

體育科 入試 實技考査種目의 妥糖性에 關한 硏究

金正默,林聖愛,李來華,金炳斗,安禹洪,李敏炯,金基學 慶北大學校 師範大學 1977 敎育硏究誌 Vol.19 No.-

The following conclusion has been derived from the foregoing results of analysis and observation. First, we found, in this proposed system, reliability, objecivity, and individuality. Second, this type of system must be able to measure numerable testees. Third, testees are regulated that they are brought to the same condition in which their willful adventage are not allowed, Fourth, when considering the foundamental physical strength and skills can be evaluated in general throughout all skills areas of physical education, the following items of skills are recognized to have the advantages and disadvantages which relatively lack or relieve one another. However, under various conditions of the prevailing system of the entrance physical test, the following measurable items of physical skills selected are concluded as having highest validity primarily for the physical entrance examination. 1. Side Step B Style 2. Zigzag Run 3. Wall Pass 4. Standing Long Jump

CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

한국인에서 도파민 D4 수용체 다형성과 Novelty Seeking 성격 특성의 연관성

이헌정,이홍석,강화연,김린,이민수,서광윤,변영찬 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.4

연구목적 : 저자들은 한국인에서 도파민 D4 수용체(DRD4) 유전자의 다형성과 성격의 관련성을 알아보고자 본 연구를 시행하였다. 방 법 : 173명의 건강한 한국인 여자 청소년(13.88±0.29세)을 대상으로 하여 성격 기질 설문지(Temperament and Character Inventory : TCI)를 작성하도록 하였으며, 이들에서 DRD4 exon Ⅲ 48-bp VNTR(variable numbers of tandem repeats) 다형성을 분석하였다. 결 과 : DRD4 exon Ⅲ Vntr에서 긴 대립유전자(≥5 repeats)를 가진 군이 긴 대립유전자를 가지지 않은군에 비하여 Novelty Seeking 척도 점수가 유의하게 높았다(t=2.11,p=0.037). 결 론 : 본 연구 결과는 DRD4-exon Ⅲ의 긴 대립유전자가 Novelty Seeking 성격과 관련이 있다는 기존의 외국에서의 연구결과를 다시금 확인해주는 것이다. Objectives : We investigated the relationship of personality traits with dopamine D4 receptor(DRD4) exon Ⅲ polymorphism in a Korean population. Methods : We analysed DRD4 exon Ⅲ 48-bp repeats polymorphism in 173 Korean heakthy female adolescents(age=13.88±0.29years) who also completed Temperament and Charater Inventory(TCI). Results : Novelty seeking score of the TCI was significantly higher in the subjects with DRD4 long alleles(≥5 repeats) compares with the subjects without these (t=2.11, p=0.037). Conclusion : The present study supports the previous reports that long repeats of the DRD4-exon Ⅲ polymorphism are relatedwith Novelty Seeking personality.

巴戟天의 實驗的 硏究(第1報)

최정화,홍석,정현우 대한본초학회 1996 大韓本草學會誌 Vol.11 No.1

These studies were carried out to investigate the effect of Radix Morindae water extract on the inhibitory contractile action of histamine in guinea pig. The results of these studies were as follows; 1. Contractile force of histamine from trachea smooth muscle in guinea pig was inhibited by Radix Morindae. 2. Radix Morindae did not changed the contractile force of histamine pretreated propranolol, indomethacin, and methylene blue. 3. Contractile force of uterus in rat was inhibited by Radix Morindae. 4. Contractile frequence of uterus in rat was inhibited by Radix Morindae.

세 도시의 생활 쓰레기 발생량의 장래 추이 예측

오정화,강호,이진홍 忠南大學校 環境問題硏究所 1995 環境硏究 Vol.13 No.-

This study centers on a long-term prediction for the generation of municipal solid wastes for 3 cities such as Pusan, Taejon and Cheongju. For the long-term prediction several statistical models based on time or population were proposed and utilized. The MSW discharge per day from 3 cities predicted is in the range of 12,100~14,600 ton for Pusan, and 3,100~3,800 ton for Taejon, and 2,200~2,600 ton for Cheongju in the year of 2008, respectively. However, the volume-based charge system since January 1995 has made a great effect on the generation of MSWs. Unfortunately, there has been few data to consider this effect for the prediction. Therefore, there has been an urgent need to collect new baseline data for the more realistic long-term prediction.

GC/NPD를 이용한 다성분 잔류농약의 동시분석

김우성,이선화,김상엽,정동윤,김재이,이영자,이홍재,정성욱,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with fiorisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD, Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.