제주 지역의 B형 간염 바이러스 유전자형

조지현,박도심,김태현,염주진,김학철,문주현,양재식 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.1

목적: HBV 감염이 무증상의 자연 회복부터 간경변 혹은 간세포성 간암까지 다양하게 나타나는 요인들로 숙주의 요인과 바이러스의 요인으로 구분되어 제시되는데, 바이러스 요인으로는 감염된 바이러스이 유형이나 염기 변이들이 논의된다. HBV 유형을 구분하는 데는 혈청형과 유전자형이 이용되는데 근자에는 주로 유전자형이 이용된다. 이러한 HBV의 분포는 지역적으로 다르나 지역에 따라서 다른 유형들이 혼재되어 있다. 동남아시아는 B형이지만 극동아시아는 C형으로 알려져 있으며, 대만과 오키나와에서는 B와 C형이 혼재되어 나타난다. 이러한 결과로 미루어 보아 우리 나라의 남부지역에서는 B과 C형이 혼재되어 있을 가능성이 있다. 이에 본 연구는 제주 지역에서 HBV의 유전자형을 조사하고자 하였다. 대상과 방법: 원적지가 제주도이면사, HBsAg가 양성이었던 107명(평균 연령: 46.5세)을 대상으로 하였다. 이들의 혈청으로부터 HBsAg/antiHBe를 분석하고, DNA를 추출하여 7개의 유전자형에 따라 특이성을 갖는 시발체(genotype specific primer)들을 이용하여 PCR로 증폭한 후 이를 분석하였다. 주된 유전자형의 일부와 다른 유형에서 증폭에 이용되었던 시발체를 다르게 이용하여 hemi nested PCR로 증폭한 후 염기를 분석하여 계통발생학적 분석법을 이용하였다. 결과: 1. PCR에 의한 HBV DNA 양성은 107명 중 59명(55.1%)이 양성이었다. HBeAg/anti HBe의 발현 양태에 따라 음성/음성에서 2명(66.7%), 음성/양성에서 30명(41.1%), 양성/음성에서 24명(85.7%), 양성/양성에서는 3명(100%)에서 증폭되었다. 2. Multiplex PCR에서는 58명의 유전자형이 C형, 1명에서는 B형과 C형이 동반 출현하였다. 3. Genotype specific primer를 이용하여 각각 따로 PCR을 시행한 경우에 A형에 의하여 증폭된 예가 1예, B형에 의하여 증폭된 예가 1예, C형에 의하여 증폭된 예가 59예로 A형과 B형 모두가 C형에서 증폭된 예였다. 4. 한 쌍의 시발체에 의하여 증폭되었던 A형과 B형 및 C형의 일부에서 염기를 분석한 결과 모두 C 유전자형이었다. 결론: HBsAg이 양성인 제주 지역 주민의 HBV의 유전자형은 C형일 것으로 생각된다. Background/Aims: Hepatitis B virus (HBV) was classified into 8 genotypes by a sequence divergence in the entire genome designated from A o H. HBV genotypes have distinct geographic distributions. Recently, HBV genotypes have been partially found as influencing the clinical manifestation of chronic liver disease in hosts. In Korea, the distribution of HBV genotypes remains unclear. The aim of this study was to evaluate the prevalence of the HBV genotype on Jeju Island. Methods: Hepatitis B virus genotypes were evaluated among 107 hepatitis B carriers residing on Jeju Island. We used single PCR and multiplex-PCR assay with genotype-specific primer pairs for HBV genotypes A-F for the genotyping. Results: 1. Fifty nine samples (55%) were positive for HBV DNA. The positivity was different according to the pattern of HBeAg/anti-HBe expression, as -/-; 2/3 (66.7%), -/+; 30/73 (30%), +/-; 24/28 (85.7%) and +/+; 3/3 (100%). 2. In the single primer set of genotype-specific PCR, 59 samples (100%) were detected as genotype C and 2 (3%) were also detected as genotype A and B. 3. In multiplex-PCR, 58 samples (98%) were detected as genotype C and only one (2%) as a mixed pattern of genotype B and C. 4. When the PCR products were amplified with universal sense and genotype specific anti-sense from one genotype A, one B, and 2 C, all were included in genotype C. Conclusions: These results suggest that on Juju Island, almost all HBV genotypes are C.(Korean J Hepatol 2004;10:42-50)

봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향

이상훈,이현종,백용현,김수영,박재경,홍승재,양형인,김건식,이재동,최도영,이두익,이윤호 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective In order to study the effects of bee venom(BV) on the pain, edema, and acute inflammatory reactant of rheumatoid arthritis(RA) patients. Methods Patients with RA who met the ACR(American College of Rheumatology) 1987 revised criteria for the diagnosis of RA were treated with the BV therapy twice a week for 3 months. Tender Joint counts, swollen joint counts, Visual analog scale(VAS), morning stiffness, ESR. C-reactive protein(CRP) were analyzed before and after BV therapy. Results The results as follows. 1. Tender joint counts in patients after BV therapy were significantly lower than those before BV therapy(9.0±7.9 vs 15± 11.4, p=0.002). 2. Swollen joint counts of the patients after BV therapy were significantly lower than those before BV therapy(50±61 vs 15±23, p=0.001). 3. VAS in patients after BV therapy was significantly lower than those before BV therapy(608± 17.6 vs 380± 159, p=0.000). 4. Duration of morning stiffness in patients after BV therapy was significantly reduced compared with that before BV therapy(119.1± 112.6 min vs 59.0±89.7 min, p=0.009). 5. ESR and CRP were not significantly changed before and after BV therapy, suggesting BV itself could make inflammatory reaction as well as therapeutic effect. Conclusions BV therapy improved tender joint counts, swollen joint counts and duration of morning stiffness in this study, and further study is needed on long-term effect of BV therapy.

Rifampicin에 의한 것으로 추정되는 위막성 대장염1예

김수현,이은우,정종혁,문승현,김동한,양혁승,오영상,김호동,김도현,박혁,박정환,박경옥,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Pseudomembranous colitis, caused by altering the normal colonic flora and allowing the multiplication of Clostridium difficile, is an deleterious adverse effect of antibiotics. But it is rarely reported by rifampicin. Rifampicin is one of the first line drug in the treatment of tuberculosis and many patients are exposed to its potential adverse effects. We experienced a patient that had abdominal discomfort and hematochezia due to pseudomembranous colitis after receiving antituberculous medication, and which was probably caused by rifampicin. A 82 years old man was admitted with abdominal discomfort and hematochezia for one week. On the past history he had been diagnosed as endobronchial tuberculosis about 4 months ago. Colonoscopy revealed multiple discrete whitish mucosal lesion on rectosigmoid colon, and histologic findings were consistent with pseudomembranous colitis. The antituberculous agents were discontinued and vancomycin was administered. The patient's symptoms were resolved within several days. There was no recurrence after reinstitution of the antituberculous agents excluding rifampicin. We report here on a case of pseudomembranous colitis probably due to rifampicin.

An electrochemical nanofilm sensor for determination of 1-hydroxypyrene using molecularly imprinted receptors

Yang, Do-Hyeon,Lee, Chang-Soo,Jeon, Beung-Hoon,Choi, Sung Mook,Kim, Yong-Dae,Shin, Jae Sup,Kim, Heon Elsevier 2017 Journal of industrial and engineering chemistry Vol.51 No.-

<P><B>Abstract</B></P> <P>A novel electrochemical nanofilm sensor for 1-hydroxypyrene (1-OHP) was developed based on a molecular imprinted TiO<SUB>2</SUB> gel matrix, which was prepared with Ti(O-<I>n</I>-Bu)<SUB>4</SUB> and 1-OHP using sol–gel technology. Cyclic voltammetry (CV) showed that guest binding on the non-imprinted sensor resulted in nearly the same current changes for all guest molecules, indicating non-specific binding onto the TiO<SUB>2</SUB> gel matrix. In contrast, binding at the molecular imprinted sensor revealed that the selectivity for 1-OHP relative to structurally related guest molecules was estimated to be 1.8–6.7 times greater at 1×10<SUP>−9</SUP> M, reflecting its specific adsorption and binding capacity for the template molecule.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Fullerene nanohybrid metal oxide ultrathin films

Do-Hyeon Yang,박철순,Ji Hye Min,Mee-Hye Oh,Yeo Seong Yoon,Seung-Woo Lee,신재섭 한국물리학회 2009 Current Applied Physics Vol.9 No.2

In this study, fullerene nanohybrid ultrathin films were prepared by the surface sol–gel process including the alternate adsorption of metal alkoxide and fullerene. The fullerene nanohybrid ultrathin films could be controlled on the nanometer thickness level, and it was confirmed that an assembly of films showed linear growth by quartz crystal microbalance (QCM) and UV–vis absorption spectra measurements. The contact angle changes dramatically when the outermost fullerene layer is changed. The contact angle systematically and reproducibly alternates from a value less than 5° to values of around 32° and 85° for the metal oxide and C60 layers, respectively. These oscillations in the contact angle continue to the same degree up to at least 10 cycles. These results indicate that the surface is uniformly covered by metal oxide and C60 during the sequential adsorption process.

Simple method for the fabrication of 1-hydroxypyrene-imprinted TiO2 gel nanofilms

Do-Hyeon Yang,Young Rok Ham,Mee-Hye Oh,Yeo Seong Yoon,신재섭,김용대,김헌 한국물리학회 2009 Current Applied Physics Vol.9 No.2

In this study, 1-hydroxypyrene (1-OHP)-imprinted TiO2 gel films were prepared by spin coating. Incorporation of the template molecules and their removal were confirmed by UV–vis absorption spectra measurements. Guest selectivity of the 1-OHP-imprinted TiO2 film was estimated to be 1.6, 1.9, and 1.4 against benzoic acid (BA), 1-naphthol (1-NA), and 4-(phenylazo)benzoic acid (4-PABA)-imprinted TiO2 film, respectively. The 1-OHP-imprinted films demonstrated greater binding to 1-OHP than the reference imprinted films. Similar results were obtained with the BA, 1-NA, and 4-PABA-imprinted films, and their guest selectivity against the reference imprinted films amounted to 1.2–3.1 times greater. This result suggests that the binding cavity of the imprinted TiO2 gel matrices discriminates between molecular size, shape, and functional groups. Although much improvement is still needed, this imprinting process is a simple, useful, and less time-consuming way to prepare discriminative receptor cavities.

배지 종류가 둥근매듭풀(Kummerowia stipulacea (Maxim.) Makino) 기내증식에 미치는 영향

임현정(Hyeon Jeong Im),최현준(Hyeon Jun Choi),송기선(Ki Seon Song),김도현(Do Hyun Kim),오범석(Beom Seok Oh),김상근(Sang Geun Kim),김양수(Yang Soo Kim),송치현(Chi Hyeon Song),이재선(Jae Sun Yi) 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

둥근매듭풀(Kummerowia stipulacea (Maxim.) Makino)은 콩과(Leguminosae) 매듭풀속(Kummerowia)에 속하는 초본성 식물로서 척박한 곳에 잘 자라며 정원용 녹화 식물로 이용된다. 본 연구는 둥근매듭풀의 대량생산을 위한 기내증식 체계 확립을 위하여 배지 종류에 따른 최적 배양 조건을 구명하고자 수행하였다. 둥근매듭풀 종자발아 조건은 양분을 첨가하지 않은 대조구와 MS(Murashige&Skoog medium), 1/2MS배지에 4주간 배양하였다. 그 결과 MS배지에서 평균발아일수가 짧았고, 모든 배지에서 80% 이상 높은 발아율을 보였으며, 특히 MS배지에서 91.4%로 가장 높았다. 둥근매듭풀 기내배양묘 배양 조건은 양분을 첨가하지 않은 대조구와 MS(Murashige&Skoog medium), B5(Gambong O.L medium), WPM(Lloyd&Mccown medium), SH(Schenk&Hildebrandt medium), N6(CHU medium), NM(Nitsch medium)배지에 4주간 배양하였다. 그 결과 배지종류에 따라 차이를 보였고, 둥근매듭풀 기내배양묘는 WPM배지에서 신초 길이 (Shoot length) 5.96cm, 엽 수(Leaf number) 32.50개로 가장 우수하였고, NM배지에서 뿌리 발생률(Root ratio)이 13.8개로 가장 높았다. 특히, N6, B5배지는 배양 2주차부터 모든 엽이 갈변하였고 줄기 생장이 정지하여 둥근매듭풀 기내배양묘 생육에 적절하지 않았으며, WPM배지는 둥근매듭풀 기내배양에 효과적이었다. 이러한 연구결과는 둥근매듭풀의 산업화 및 대량증식을 위한 기초자료로 활용될 수 있을 것으로 판단된다.

콩기름을 이용한 폴리우레탄 포옴의 합성

양도현(Do Hyeon Yang),이광용(Kwang Young Lee),신재섭(Jae Sup Shin) 한국공업화학회 1999 공업화학 Vol.10 No.5

N/A N/A

식물생장조절제가 털부처꽃(Lythrum salicaria) 선발계통 WH1, PI1의 기내증식에 미치는 영향

임현정(Hyeon Jeong Im),송치현(Chi Hyeon Song),최현준(Hyeon Jun Choi),송기선(Ki Seon Song),김도현(Do Hyun Kim),오범석(Beom Seok Oh),김상근(Sang Geun Kim),김양수(Yang Soo Kim),이재선(Jae Sun Yi) 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

털부처꽃(Lythrum salicaria)은 부처꽃과(Lythraceae) 부처꽃속(Lythrum)에 속하는 다년생 초본식물로서 주로 습지에 자라며, 여름(7~8월)에 붉은 자주색의 꽃이 총상꽃차례로 피어 관상용으로 재배한다. 본 연구는 털부처꽃 선발계통인 WH1와 PI1의 대량생산을 위한 기내증식 체계 확립을 위하여 생장조절제 종류에 따른 기내배양 조건을 구명하고자 수행하였다. 털부처꽃 선발계통 기내배양묘 WH1과 PI1의 배양 조건은 MS 기본배지에 3% Sucrose를 넣고 pH 5.8로 조정한 후 0.4% Gelrite를 첨가하였고, 처리 조건은 생장조절제를 처리하지 않은 대조구와 0.5, 1.0 mg/L BAP와 Kinetin을 0.5mg/L IAA 및 GA3에 혼용하여 4주간 배양하였다. 털부처꽃 선발계통 WH1, PI1은 생장조절제의 영향이 있으며, 생장조절제 종류와 농도에 따라 차이를 보였다. WH1 및 PI1은 0.5mg/L IAA와 Kinetin에서 신초길이(Shoot length) 12.22cm, 11.43cm로 가장 길었으며, 신초수(Shoot number)는 WH1은 0.5 mg/L IAA, 1.0 mg/L Kinetin 혼용처리에서 2.56개, PI1은 0.5 mg/L IAA, 0.5 mg/L mg/L Kinetin 혼용처리에서 4.10개로 가장 많았다. 마디수(Node number)는 WH1은 0.5 mg/L IAA, 0.5 mg/L Kinetin 혼용처리에서 6.89개, PI1은 0.5 mg/L IAA, 1.0 mg/L Kinetin 혼용처리에서 10.10개로 가장 많았다. 뿌리 및 엽 수(Root·Leaf number)는 WH1은 0.5 mg/L IAA, 0.5 mg/L Kinetin 혼용처리에서 13.22개, 15.44개, PI1은 0.5 mg/L IAA 단용처리에서 21.00개, 13.10개로 가장 많았다. 특히, GA3 처리의 경우, 선발계통 모두 줄기와 잎이 왜소하고 도장하여 증식용 배지로는 효과적이지 않았으며, 털부처꽃 선발계통인 WH1와 PI1의 증식용 배지에는 식물생장조절제 IAA와 Kinetin을 혼용하여 사용하는 것이 적절한것으로 판단된다. 이러한 연구결과는 털부처꽃의 신품종 육성 및 대량증식을 위한 기초자료로 활용될 수 있을 것으로 판단된다.

KAAACI Allergic Rhinitis Guidelines: Part 2. Update in Non-pharmacological Management

Park Do-Yang,Lee Yong Ju,Kim Dong-Kyu,Kim Soo Whan,Yang Hyeon-Jong,Kim Do Hyun,Jun Young Joon,Park Sang Chul,Kim Bong-Seong,Yang Song-I,Lee Il Hwan,Kim Minji,Ryu Gwanghui,강성윤,Kim Mi-Ae,이상민,Kim Hyun-Ju 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.2

Allergic rhinitis is the most common chronic disease worldwide. Various upper airway symptoms lower quality of life, and due to the recurrent symptoms, multiple treatments are usually attempted rather than one definitive treatment. There are alternatives to medical (medication-based) and non-medical treatments. A guideline is needed to understand allergic rhinitis and develop an appropriate treatment plan. We have developed guidelines for medical treatment based on previous reports. The current guidelines herein are associated with the “KAAACI Evidence-Based Guidelines for Allergic Rhinitis in Korea, Part 1: Update in pharmacotherapy” in which we aimed to provide evidence-based recommendations for the medical treatment of allergic rhinitis. Part 2 focuses on non-pharmacological management, including allergen-specific immunotherapy, subcutaneous or sublingual immunotherapy, nasal saline irrigation, environmental management strategies, companion animal management, and nasal turbinate surgery. The evidence to support the treatment efficacy, safety, and selection has been systematically reviewed. However, larger controlled studies are needed to elevate the level of evidence to select rational non-medical therapeutic options for patients with allergic rhinitis.