들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

고빌리루빈혈증을 동반한 자가면역성 간염 1례

서영범,김성욱,장재식,강혁주,이중현,윤병구,김욱년,이광헌,이구,유석동,양창헌,이정호,이영현,이창우,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

자가면역성 간염은 대개 만성 경과를 가지며, 혈중 자가면역항체와 혈청 글로불린치의 상승, 그리고 조직학적으로 괴사 염증성 변화를 특징으로 하는 질환으로 아직 정확한 병인이 밝혀져 있지 않은 상태이다. 발병연령은 대개 젊은 영자(15-25세)에서 호발한다. 이 질환은 급성 간염의 임상경과를 보일 수 있으나, 심한 급성 간염이나 전격성 간염으로도 나타날 수 있는데, 이 경우 아주 나쁜 예후를 보인다고 한다. 치료는 자가면역성 간염 임상 경과의 다양성이나 병인, 병리기전의 불확실성에도 불구하고 대개 steroid 치료에 반응하는 것으로 알려져 있으며 대개 80%의 관해율을 나타내며 궁극적으로 간경변으로의 진행을 막을 수 있는 것으로 보인다. 저자들은 58세 남자에서 발생한 급성의 경과를 가지고 심한 황달을 동반한 자가면역성 간염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Autoimmune hepatitis (AIH) is a chronic necroinflammatory liver disorder of unknown cause associated with circulating autoantibodies and a high serum globulin level. The age of onset of AIH show a peak between the age of 15 and 25 years. AIH can develop and be manifested as acute hepatitis, but severe form of acute hepatitis or fulminant hepatic failure has a poor prognosis. Although AIH is likely to progress from chronic active hepatitis to cirrhosis, steroid therapy can control the disease activity, prolong survival, improve the quality of life , and defer liver transplantation. In the present report we describe a 58-year-old man who admitted because of progressive jaundice and fatigue. He was diagnosed with AIH from laboratory test result showing positivity for antinuclear antibodies, anti-smooth muscle antibodies, and negativity for hepatitis viral markers and from liver biopsy. Steroid therapy, oral administration of prednisolone, was effective in improving the liver function test. Following liver biopsy 6 months after onset shows markedly improved necroinflammatory activity.

Negative Regulation of Hypoxic Responses via Induced Reptin Methylation

Jason S. Lee,김윤호,김익수,김보규,최희준,이지민,신희재,김정화,김지영,서상범,이호,Olivier Binda,Or Gozani,Gregg L. Semenza,김민형,김근일,황대희,백성희 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

Negative Regulation of Hypoxic Responses via Induced Reptin Methylation

Jason S. Lee,김윤호,김익수,김보규,최희준,이지민,신희재,김정화,김지영,서상범,이호,Olivier Binda,Or Gozani,Gregg L. Semenza,김민형,김근일,황대희,백성희 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

韓國産 夜行性 맵시벌科(벌目: 벌亞目)의 分類學的 硏究

이종욱,정종철,김기범,김정한,배지득,남상호 대전대학교 기초과학연구소 2002 自然科學 Vol.13 No.2

영남대 생물학과에 소장되어 있는 표본과 2001년 4월~2002년 6월 사이에 채집한 표본을 대상으로 하여 한국산 야행성 맵시벌의 분류학적 연구와 출현 현황 및 숙주 관계의 분석을 실시하였다. 분류학적 연구 결과 Gauld(1970)가 영국산 야행성 맵시벌을 대상으로 한 연구에서 기록한 Mesochorinae아과, Tryphonin-ae아과, Ctenoplematinae아과, Ophioninae아과의 4아과외에 Metopinae (Metopius속)를 추가하여 한국산 야행성 맵시벌류는 총 5아과 13속을 기록하였다. 또한 한국산 야행성 맵시벌의 출현 현황을 분석한 결과 야행성으로 잘 알려진 Mesochorinae아과의 Mesochorus속, Tryphoninae아과의 Netelia속과 Ophioninae아과가 전체 야행성 맵시벌 증 95%를 차지하였다. 속별 출현현황을 살펴보면 Netelia속이 393개체가 출현하여 가장 많은 수를 나타내었으며 Ophi-on속, Mesochorus속, Enicopilus속도 전체 출현 분류군 중 높은 비중을 차지하였다. 월별 현황을 살펴보면 전반적으로 4월에서 11월 사이에 걸쳐 나타나는데, Op-hioninae아과는 5월과 7월에, Tryphoninae아과는 6월에 집중적인 출현 빈도를 보였다. 숙주의 분류군에 따른 야행성 맵시벌의 기생 양상을 살펴보면 대부분이 나비목을 대상 숙주로 하고 있으며 Mesochorinae아과와 Ctenopelmatinae아과는 벌목에도 많은 종이 기생하는 것으로 나타났다. 특히 Mesochorinae아과의 Cidaphus속은 위생 해충으로 분류되는 기생파리과(Tachinidae)에도 기생하는 것으로 나타나 위생 해충을 구제할 수 있는 천적 분류군으로 연구될 수 있는 가능성을 보였다. 숙주의 식성에 따라 살펴보면 부식성의 먹이를 섭식하는 부식성(saprophagus) 숙주, 곤충의 유충을 섭식하는 식충성(entomophagus)인 숙주가 소수 출현하였고 기생생활(parasite)을 하는 숙주도 다수 출현하였으나 대부분의 야행성 맵시벌의 기생 대상 숙주는 주로 식물을 해하는 식식성 곤충(phytophagus)으로 나타났다. Nocturnal Ichneumonidae, which was called yellow-brown ichneumonoid, included several subfamilies adapted for nocturnal activity. Taxonomic study of nocturnal ichneumonoid has been investigated by Gauld(1975) but examination of Korean nocturnal ichneumonoid has not been provided until now. This present study: the taxonomic revision, Appearance state and Host-parasite relation analysis of nocturnal ichneumonoid, were examined using Korean peninsula specimens. As the result. approximately 13 genera referable to 5 subfamilies appeared regularly in light trap. Genus MetopiudSubfamily Illetopinae) were newly recorded in nocturnal ichneumonoid. Tryphoninae and Ophioninae, which were dominant in appearance taxa! occupied 96% of total numbers of individuals, and majority of them were taken from May to November. Nocturnal ichneumonoid are parasitoids of part of immature holometabolous insects(Coleoptera, Diptera, Hymenoptera, Lepidoptera). Lepidoptera is the most common hosts of nocturnal group except Mesochorinae and Ctenopelmatine. In large number of Mesochorinae are koinobiont hyperparasitoids of ectoparasitic or endoparasitic BraconiddHymenoptera), and, less frequently, of Tachinidae(Diptera1. Also, Ctenopelmatinae are koinobiont endoparasitoids of Symphyta and, rarely, Lepidoptera. Majority of nocturnal ichneumonoid hosts are phytophagous, feeding plant taxa, and many host of Mesochorinae taxa are parastoids, and a few host are saprophagus or entomophagus.

공공건설프로젝트에서 효율적인 건설분쟁처리에 대한 연구

이상범,조영준,김정길 동의대학교 산업기술개발연구소 2005 産業技術硏究誌 Vol.19 No.-

A In Korea when change order was occurred, contract amount may be adjusted according to Government Contract Act. If the requirement of privy is fully accepted, conflict can't arise. Because of a misunderstanding and misappropriation of contract, a difference between the contract condition and job site, an errors & omissions of the design document, the contract disputes has been increased since the Seoul 2nd Metro Project claims alleged by 6 Contractors at Feb. 1998. To mitigate and to resolve effectively construction dispute, training of construction participants, introduction of coercive arbitration clause in public construction, adaption of the lowest bidding system, participation of construction engineer in arbitration process were suggested in this paper.

2종의 뒤영벌 여왕벌의 실내 정착율 비교

이상범,배태웅 東亞大學校 1999 東亞論叢 Vol.36 No.-

국내종 뒤영벌족 중 주요 우점종인 호박벌과 좀뒤영벌의 실내정착가능성을 조사하기 위하여 1997년 3월말에서 5월초까지 수원, 지리산과 인천영흥도에서 채집한 두 종의 여왕벌을 상자별로 사육한 결과 다음과 같은 결과를 얻었다. 호박벌과 좀뒤영벌 모두 상자의 재질이나 크기에 따른 산란율 및 성충출현율의 차이는 없었으나 호박벌이 좀뒤영별보다 산란율, 첫일벌 출현율, 50두 이상 일벌출현율, 새여왕벌 출현군체수, 여왕벌 수명 및 군체의 크기 등에서 좋은 성적을 보여 실내사육이 가능한 종이며 방화성 곤충으로서도 개발이 가능한 종이다. For survey of colony performance by foundation queens collected, collection of domestic species of Bombini was made from March to May of 1997 on Kyungki Suwon, Kyungnam Chiri-Mt. Area and Inchun Younghwung-Do in Korea. And queens of two Bombus species was reared at the 4 kinds of wooden case. The results obtained are as follows; Both of Bombus ignitus and B. ardeus were not a difference between the rate of egg-laid and of warker emergence according to the quality of material and size of all of ages. Bombus ignitus is better than Bombus ardens according to the rate fo egg-laid, rate of first worker emrgence, ≥50 heads of worker emergence, rate of new queen emergence, longivity of foundation queens, and size of colonies. So B. ignitus is possible to develop indoor rearing and foraging insect.

알긴산나트륨 및 첨가제를 함유한 서방성 매트릭스 정제

이범진,이태섭,신성이,허보욱,유승구 한국약제학회 1996 Journal of Pharmaceutical Investigation Vol.26 No.3

The matrix tablet containing sodium alginate and CaHPO₄ can release drugs in a controlled fashion from hydrogel with gelling and swelling due to their interaction as water penetrates the matrices of the tablet. The purpose of this study was to evaluate release characteristics of the matrix tablet varying the amount of sodium alginate, CaHPO₄ and other excipients such as chitosan, hydroxypropyl methylcellulose (HPMC) and Eudragit^ⓡ RS100 in the simulated gastric and intestinal fluid. The practically soluble ibuprofen was used as a model drug. The release profiles of matrix tablet in the gastric fluid as a function of sodium alginate/CaHPO₄ ratio was not pronounced because of low solubility of drug and stability of alginate matrices. However, release rate of drug from the matrix tablet in the intestinal fluid was largely changed when sodium alginate/CaHPO₄ ratio was increased, suggesting that the ratio of sodium alginate/CaHPO₄ was an important factor to control the gelling and swelling of the matrix tablet. The incorporation of other excipients into the matrix tablet also influenced the release rate of drug. The chitosan and HPMC decreased the release rate of drug. No release of drug was occurred when Eudragit^ⓡ RS100 was added into the tablet. The retarded release of matrix tablet when excipients were added resulted from the hindrance of swelling and gelling of the matrix tablet containing sodium alginate and CaHPO₄. The hardness and bulk density of the matrix tablet was not correlated with release rate of drug in the study. From these findings, the ratio of sodium alginate and CaHPO₄ in the matrix tablet in addition to incorporation of excipients could be very important to control the release rate of drug in dosage form design.