제초제저항성 들잔디(Zoysia japonica Steud.) 이벤트 Jeju Green21의 환경위해 성평가

배태웅,강홍규,송인자,선현진,고석민,송필순,이효연 한국식물생명공학회 2011 식물생명공학회지 Vol.38 No.2

Transgenic zoysiagrass (Zoysia japonica Steud.) expressing the bar gene inserted in the plant genome has been generated previously through Agrobacterium tumefaciensmediated transformation. The GM zoysiagrass (event: JG21) permits efficient management of weed control of widely cultivated zoysiagrass fields, reducing the frequency and cost of using various herbicides for weed control. Now we have carried out the environmental risk assessment of JG21 prior to applying to the governmental regulatory agency for the commercial release of the GM turf grass outside of test plots. The morphological phenotypes, molecular analysis, weediness and gene flow from each test plot of JG21 and wild-type zoysiagrasses have been evaluated by selectively analyzing environmental effects. There were no marked differences in morphological phenotypes between JG21 and wild-type grasses. The JG21 retained its stable integration in the host plant in T1 generation, exhibiting a 3:1 segregation ratio according to the Mendelian genetics. We confirmed the copy number (1) of JG21 by using Southern blot analysis, as the transgenic plants were tolerant to ammonium glufosinate throughout the culture period. From cross-fertilization and gene flow studies, we found a 9% cross-pollination rate at the center of JG21 field and 0% at distances over 3 m from the field. The JG21 and wild-type zoysiagrass plants are not considered “weed” because zoysiagrasses generally are not dominant and do not spread into weedy areas easily. We assessed the horizontal gene transfer (HGT) of the transgene DNA to soil microorganisms from JG21 and wild-type plants. The bar gene was not detected from the total genomic DNA extracted from each rhizosphere soil of GM and non-GM Zoysia grass fields. Through the monitoring of JG21 transgene’s unintentional release into the environment, we found no evidence for either pollen mediated gene flow of zoysiagrass or seed dispersal from the test field within a 3 km radius of the natural habitat.

방사선 조사에 의해 유도된 돌연변이 들잔디의 형태적 특성 연구

배태웅,송서영,송인자,전영주,강지연,윤필용,배창휴,류기중,이효연 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.38 No.1

SO₂ gas 가 釜山近郊의 街路樹에 미치는 影響

裵泰雄,韓熙世 동아대학교 환경문제연구소 1984 硏究報告 Vol.7 No.2

The sulfur contents in leaves of street trees in Pusan city showed a remarkably higher value than those of the leaves in the non-polluted areas. Popular and Firmiana platanifolia showed the highest sulfur contents of leaves whereas those of Ginkgo bi-lola showed the least values. The sulfur contents in the leaves of the needle leaf tree in Busan city were higher than of those of the broad leaf tree. The leaves picked on August on may and November showed higher sulfur contents than those of leaves picked.

GMO 격리포장에서 제초제저항성 돌잔디 (Jeju Green21)의 유전자이동 (Gene flow) 연구

배태웅,강홍규,송인자,정옥철,김주성,임평옥,송필순,이효연 한국육종학회 2008 한국육종학회 학술발표회 발표요지 Vol.40 No.1

유전자변형 제초제저항성 들잔디 (Zoysia japonica)의 위해요소억제 방안

배태웅,정옥철,강홍규,송인자,임평옥,이효연 한국육종학회 2011 한국육종학회 학술발표회 발표요지 Vol.43 No.-

南部地方에 있어서 끝동매미충의 個體群動態에 관한 硏究

裵泰雄 圓光大學校大學院 1986 學位論叢 Vol.16 No.-

The population dynamics of the green rice leafhopper, Nephotettix cincticeps UHLER, an important vector of the rice dwarf virus, were studied based on the three years' field investigations carried out at Kimhae , Milyang, Chinju and Suncheon in southern rice cultural areas. 1. The densities of the total arthropodan populations as well as the green rice leafhopper seemed to have close relation to the complexity of the environment : the topograpy, floristic components and others. the percents of the number of the N. cincticeps to the total arthropodan population were 30.5% in Suncheon, 29.5% for the Milyang and 28.2% for the Chinju , respectively. 2. Alopeculus aegurilis was found to be an important host plant for the overwintering populations of N. cincticeps. 3. More than 89-90% of the overwintering nymphs were 4th instar, and their physiological development seemed to begin in early March. 4. The alternation of the overwintering N. cincticeps in the levee of the paddy field was an average of 30.0 -36.7% in Kimhae and Suncheon areas (1984 -1985) . 5. The time of adult emergence was mid-March and the days of 50% adult emergence were 4 -10 of April. The average sex ratio between male and female was 44:55 under field cage condition. 6. Egg maturing peak period of N. cincticeps in the southern area was from late April to early May in the overwintered generation and mid-June in the lst generation. 7. The peak occurrence times of each age in the fallow were May 1O(l~2nd), May 25 (3rd), May 30(4th),June 10(5th) and June 15(adult), respectively. 8. The overwintered nymphs at levees moved to fallow at the beginning of early February and the peak time of moving was at the beginning of early March. 9. The peak of adult in the nursery bed was on 10-14 of june (male) and 16 -20 of june (female). 10. The peak occurrences of overwintered, lst, 2nd, 3rd and 4th generations were early April, mid-June, late July, late August and late September, respectively. 11. The occurrence of N. cincticeps had one more extra generation at the early transplanting field than at the late transplanting field. 12. Each generation nymphs were field-collected and reared on rice and water -foxtail seedling in a wood cage. The 50% adult emergence date of each generation were early April (overwintered generation), early June (1st generation), late July (2nd generation) and early September (3rd generation), respectively. 13. Emergence period of the 1st generation of adult was from late May to early June. The emergence up to 50% was observed to be from 7-11 of June. 14. The study on the effectiveness of the insecticides to the N. cincticeps in paddy field showed that Carbofuran granules was highly effective for the control of the N. cincticeps. 15. Parasitism rates of dorialaid flies which was natural enemy of N. cincticeps were 20-40% in March and April, and 40-58.4% in June and September. 16. The 3rd generation (59.1 %) of N. cincticeps showed higher occurrence of egg parasite than that of the other generation. 17. The egg parasite rate of N. cincticeps was 59.1 % between late April and early May, the peak of host egg deposition period in fallow.

釜山地方의 土壤徵小節足運動 分布調査에 관한 硏究(Ⅰ)

裵泰雄 東亞大學校 1983 東亞論叢 Vol.20 No.3

우리나라 南部地方인 부산지역의 密度와 場所에 따른 微小土壤節足動物에 관한 調査를 實施한 바 다음과 같은 結果를 얻었다. 1. 범어사.통도사에서는 Acarina와 Collembola가 大部分이었으며 Acarina에는 Oribatei가 51.1%로 主種을 이루었다. Collembola는 8, 10地點과 13,14,15地點이 각각 優勢하엿다. 2. 태종대에서는 Acarina보다 Collembola가 많았으며 Collembola에는 Isotomidae가 69.1%로 가장 많았고 7,8,9,10,12地點이 優勢하였다. 3. 美小土壤節足動物의 全個體中 Acarina와 Collembola가 96.4%로 大部分을 차지하였으며 其他의 土壤動物은 3.5%에 불과하였다. 4. 以上의 實驗 結果에 의하여 有機物 咸量, 土壤濕度, pH에따라 土壤動物의 分布量에 많은 差異가 나타났으며 好酸性에 Collembola 의 集團棲息도 확인되었다. This study was corducted to investigate the density and location on the soil micro-arthropods in south-parts of Korea. results were obtained as follows; 1. At Mt But-ae and Tong--do temple. Acari and collembola was the most abundant orbatei in acari was the most abundant and they occupied the percentage of 51.1%, Collembola was respectively dominant at the collected sites 8.10 (Bum-ae temple) 13,14,15 (Tong-do temple) 2. At tae-Jong Dae Collembola was dominant than Acari isotomidae in collembola was the the most dominant with 69% (the collected sites 7,8,9,10,12,) 3. Acari and collembola of the total individuals of soil mico-arthropods was occupied of 96.4% and other soil microartiropods amounted to 3.5%. 4. According to organic material soil moisture pH, the invidual numbers of soil insect wase very variant. collective in habitation of acidophilic collembola was also comformed.

결정적 서비스 질을 보장하는 회선 교환 위성 망의 동적 대역폭 할당에 대한 성능 분석

배태웅,이정규,Pae, Tau-Ung,Lee, Jong-Kyu 대한전자공학회 2001 電子工學會論文誌-TC (Telecommunications) Vol.38 No.6

본 논문은 망에 대한 유연성과 망의 성능의 효율성을 개선하기 위하여 회선 교환 위성 망에서 데이터 트래픽을 동적으로 다루는 시스템을 제안하고 제안한 시스템에 대한 성능을 분석한다. 제안한 시스템은 각 연결대한 용량 변경 요구를 동적으로 허락하기 때문에 그 연결 자체의 초기화 설정 및 연결을 해제하는 다른 별도의 알고리즘이 필요 없이 기존의 회선 교환 위성 망보다 효율적이다. 또한, 서비스 질을 결정적으로 보장함으로 망의 유연성 및 신뢰성을 높인다. 트래픽 소스는 표준 듀얼 리키 버켓에 의해 일정하게 조정된 트래픽을 사용하며, 이러한 트래픽을 이용하여 제안한 시스템의 성능을 해석적으로 분석하고, 이를 시뮬레이션을 통해 검증한다. 이러한 분석 결과는 앞으로 회선 교환 위성 망에서 프로토콜의 설계 및 구현 시 유용하게 사용될 수 있을 것으로 사료된다. In this paper, we propose and analyze a more efficient and flexible dynamic data traffic system for a circuit switched satellite network. Our proposed system is more efficient than existing circuit switched satellite networks and allows for dynamic capacity in each connection without rebuilding or resetting the connection software or algorithms. We also discuss an algorithm for bandwidth allocation that provides deterministic quality of service guarantees. The traffic sources are regulated using standard dual leaky buckets; the system performance is analytically evaluated; and the algorithm is verified through simulation. Our analysis scheme and results should prove useful for the design and implementation of protocols in future circuit-switched satellite networks.

한국 남부지방에 있어서 끝동매미충 개체군 동태에 관한 연구 -휴반에 있어서 개체군동태-

배태웅 한국곤충학회 1985 Korean journal of entomology Vol.15 No.2

본 연구는 우리나라 남부지방에 있어서 벼에 오갈병과 흡즙을 하여 많은 피해를 주고 있는 끝동매미충에 대한 월동개체군 및 본답에 있어서 개체군동태를 구명하여 끝동매미충을 효율적으로 제거할 목적으로 1982~1985년 사이에 김포, 밀양, 진주 및 순천등 끝동매미충 발생지에서 조사한 결과를 보고하는 바이다. 1. 시험기간중의 각 지역별 휴반에 있어서 절족종물의 월동밀도중에 끝동매미충은 전체밀도의 비율은 순천이 30.5%, 밀양, 진주가 각각 29.5%, 28. 2%를 차지하였다. 2. 끝동매미충의 월동기간중에 기생식물이 되는 것은 둑새풀이었다. 3. 휴반에 있어서 끝동매미충의 월동기간중 사망률은 김해와 순천에서 평균 65.5~67.3%였다. 4. 휴반에서 채집한 월동 끝동매미충 약충을 시험관내의 둑새풀 묘에 접종하여 사육후 누적우화율을 조산한 결과 5령 50%, 우화일은 3월 10일, 성충 50% 우화일은 4월 10일 이었다. 5. 휴반에서 월동한 끝동매미충의 약충은 2월 상순부터 휴한답으로 이동하기 시작하여 3월 상순경에는 이동의 Peak를 보였다. 6. 제1세대 성충의 우화시기는 5월 하순~6월 상순이였다. 그리고 성충 50%, 우화율의 범위는 6월 7일~11일 이었다. 7. 반부지방에 있어 끝동매미충 자웅의 난성숙 Peak는 월동세대가 4월 하순~5월 상순, 제1세대가 7월 중순 이였다. 8. 나무 Cage 내에서 둑새풀과 벼 유묘로 각 세대별충을 채집하여 접종사육후 성충 50%, 우화일을 조사한 결과 월동세대는 4월 상순, 제1세대 6월 상순, 제2세대는 7월 하순 및 제3세대는 9월 상순경에 각각 우화가 이루어 졌다. 1. The densities of the total arthropodan populations as well as the green rice leafhopper seemed to have close relation to 4he complexity of the environment; the topography, floristic components and others, The percent of number of the N. cincticeps to the total arthropodan population were 30.5% in Sunchen, 29.5% for the Milyang fad 28.2% for the Jinju. 2. The Igrasses, especially Alopecalus aequrilis, seems to be an important host plant for the overwintering populations of N. cincticeps. 3. The mortality of the overwintering on N. cinrticeps the population: Dynamic in the levee of the Paddy field was an average of 65.5∼67.3% in Kimhae and Sunchen areas (1984∼’85). 4. Accumulated percent of the development of the overwintered N. cincticeps wen the levee field collected insects were on the water-foxtail seedling in test lutes, the accumulated percents of the 5 the instar nymphs become greater than 50% by March 10. The adults began to emerge on April 2 and more than 50% of the nymphs developed in to the adults on April 14 for Mil-yang April 10 for Kimhae, April 18 for Jinju and April 10 for Sunchen. 5. The overwintered N. cincticeps nymphs at levees moved to fallow from at the beginning of early February and the peak time of moving was at the beginning of early March. 6. Emergence period of the 1st generation of adult N. Cincticeps was from late May to early June. The emergence of about N. cincticeps up to 50f was observed from 7-11 of lune 7 Egg maturing peak period of N. cincticeps in the Souther area, overwintered N. cincticeps was from late April to early May, and the 1st generation was mid-June 8. Wen field Collected each generation nymps of N. cincticeps were read on rice and water foxtail seedling in a wood cage, the 50% adult emergence date of each generation were early April(overwintered generation), early June (1st generation), early July (2nd generation) and early September (3rd generation) respectively.

GMO 격리포장에서의 유전자변형 들잔디로부터 토착미생물로의 수평유전자전달 평가

배태웅,이효연,류기현,이태형,임평옥,윤필용,박신영,류기중,송필순,이용억,Bae, Tae-Wung,Lee, Hyo-Yeon,Ryu, Ki-Hyun,Lee, Tae-Hyeong,Lim, Pyung-Ok,Yoon, Pill-Yong,Park, Sin-Young,Riu, Key-Zung,Song, Pill-Soon,Lee, Yong-Eok 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.1

The release of genetically modified organisms ($GMO_{s}$) into the environment has the potential risks regarding the possibility of gene transfer from $GMO_{s}$ to natural organisms and this needs to be evaluated. This study was conducted to monitor the possible horizontal gene transfer from herbicide-resistant zoysiagrass (Zoysia japonica Steud.) to indigenous microorganisms. We have first examined the effect of field-released GM zoysiagrass on the microbial flora in the gut of locust (Locusts mlgratoria). The microbial flora was analyzed through determining the 165 rDHA sequences of microorganisms. The comparison of the microbial flora in the gut of locusts that were captured at the field of GM zoysiagrass and of wild-type revealed that there is no noticeable difference between these two groups. This result indicates that the GM zoysiagrass does not have negative impact on microbial flora in the gut of locust. We then investigated whether the horizontal gene transfer occurred from GM zoysiagrass to microbes in soil, rhizosphere and faecal pellets from locusts by utilizing molecular tools such as Southern hybridization and polymerase chain reaction (PCR). When the total DNAs isolated from microbes in GM zoysiagrass and in wild-type zoysiagrass fields were hybridized with probes for bar or hpt gene, no hybridization signal was detected from both field isolates, while the probes were hybridized with DNA from the positive control. Absence of these genes in the FNAs of soil microorganisms as well as microbes in the gut of locust was further confirmed by PCR. Taken together, our data showed that horizontal gene transfer did not occur in this system. These results further indicate that frequencies of transfer of engineered plant DNA to bacteria are likely to be negligible.