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실험적으로 일이킨 백서에서의 바이러스성 심근염에 대한 연구
박언휘(Eun Hwi Park),김흥주(Heung Joo Kim),김성진(Sung Jin Kim),방인숙(In Sook Bang),박성진(Seong Jin Park),구정완(Jung Wan Ku),윤성철(Sung Chul Yun),한동선(Dong Sun Han),정준호(Joon Ho Jung),김성숙(Sung Sook Kim) 대한내과학회 1993 대한내과학회지 Vol.45 No.1
N/A Background: The authors injected EMC virus (Encephalomyocarditis virus) to induce viral myocarditis in ICR mice in order to study the survival rate and serial histopathologic change of viral myocarditis according to duration. Methods: Seventy adult male ICR mice of 8 to 10 weeks were used for experiment. Among 70 mice, 40 mice (Group I) were used to observe survival and 30 mice (Group II) were used to see serial histopathologic change (Each 5 mice were killed before and 5, 10, 20 days after virus injection). The hearts of animals were fixed in 10% buffered formalin and routinely processed. Hematoxylin & Eosin stain and Masson trichrome stain were done to observe inflammation and fibrosis. Results: 1) In Group I, 1 mouse died at each 2, 4, 5, 6 day after virus injection, and 4 mice died at 7 day and 2 mice died at 10 and 11 day. Total 10 animals among 40 died (mortality: 25%), and showed peak death period at 4-7 days, 2) In Group II, at 5th, 10th, 20th day after virus injection, average inflammatory grade in the myocardium were 1.8, 3.4, 2.0, respectively. 3) Histologic findings revealed monouclear cells infiltration, myofiber necrosis & degeneration. These changes occur multifocally rather than diffusely. Inflammation was most severe at 10 days. 4) Fibrosis was increased at 20 days after virus injection compared to early phase of myocarditis. Distribution of fibrosis is relatively parallel with the area of inflammation, but not with the degree of inflammation. 5) In some cases, dilation of both atriums and ventricles and thrombosis accompaning severe pulmonary congestion and edema were observed, indicating possible transition to dilated cardiomyopathy. Conclusions: It is considered that this experimental animal model may be useful not only to study the pathogenetic mechanisms of viral myocarditis but also to observe clinical course and evaluate the effect of possible drugs to treat myocarditis.
비후된 심근에서의 유세포 분석을 통한 Nuclear DNA 의 관찰
김성진(Sung Jin Kim),방인숙(In Sook Bang),박언휘(Eun Hwi Park),김홍주(Heung Joo Kim),김도헌(De Heon Kim),이예봉(Ye Bong Lee),윤성철(Sung Chul Yun),한동선(Dong Sun Han),김성숙(Sung Sook Kim) 대한내과학회 1993 대한내과학회지 Vol.45 No.2
N/A Background: During the development of the cardiac hypertrophy, cytoplasmic contents of cardiac myocyte usually increase, but the changes in the nucleus of myocytes are not well understood. Therefore, we used flow cytometry to study the changes of nuclear DNA in cardiac hypertrophy from human autopsy heart tissue. Besides, we also tried to see the DNA ploidy of developing heart of fetus. Method: Nine hypertrophied human hearts ranging from 350-620 g (Group II: 350-500 g, Group III: > 500 g) and 9 control hearts (Group I: 250-350 g) which we could obtain after the legal autopsy were studied with flow cytometric analysis. And 3 fetal hearts of 20-24 gestational weeks (Group IV) were also studied. Nuclear DNA was analyzed with FACScan (Becton-Dickinson Co.) after tissue preparation using Modified Hedley and Vindelov Method and staining with Krishan staining buffer. Results: In control hearts (Group I), Flow cytometric analysis showed normal Diploidy pattern in all tissue. However, in cardiac hypertrophy, Non-Diploidy pattern was predominant (3 out of 5 specimen in Group II, and all 4 specimen in Group III) and Tetraploidy was usually observed in this abnormal ploidy pattern, but Aneuploidy was also seen in 2 cases of severe hypertrophy of Group III. Proliferative indexes increased progressively in Group I, II, and III (17.8±5.55, 24.8±7.4%, and 36.4±5.2%, respectively) (p<0.05 between Group I and II, and p<0.01 between Group I k III). Normal growing heart tissue of fetus showed normal Diploidy pattern. Conclusions: In cardiac hypertrophy, abnormal nuclear DNA ploidy pattern can be obseved. This finding might say that, during the genesis of cardiac hyper- trophy, not only cytoplasmic change but also DNA synthesis in the nucleus occurs, but G2 phase arrest happens without further mitosis.
원발성 간암환자에서 관찰된 99mTc - DISIDA의 비장 섭취
윤성철(Sung Chul Yun),이예봉(Ye Bong Lee),김성진(Sung Jin Kim),박언휘(Eun Hwi Park),방인숙(In Sook Bang),김홍주(Heung Joo Kim),이원재(Won Jae Lee) 대한핵의학회 1993 핵의학 분자영상 Vol.27 No.1
We report a case of hepatorna which had a suggested splenic metastasis in a 64 year-old male patient. Hepatoma proved by abdominal CT (Cornputed Tomography) and considerable accumulation of 99mTc-DISIDA was noted in a enlarged sp]een. We suggests the splenic uptake of the radionuclide corresponded to the diffuse metasfasis of hepatorna to spleen.
박언휘,배용철,박매자,홍해숙,조희중,주강 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.4
In order to investigate the ultrastructure of the articular disc of the rat mandibular joint, the articular discs of aduct rats were studied by light and electron microscope. The findings were as follows. Most cells of the articular disc of the madibular joint were chondroid cells, and were arranged irregularly in anterior and posterior portions, but in middle portion, two or three cells were arranged linearly, and they were encapsulated by territorial matrix. The nacleus of chondroid cell was irregular shape or binucleated, and pyknosed occasionally, the nucleus fibrous lamina was developed, however the rough endoplasmic reticulum, mitochondria, lysosome, and golgi apparatus were not well developed, and many partitions of the cytoplasm were, filled with intracytoplasmic microfilaments. The micropinocytotic vesicles lining the cell membrane were not well developed, two or three lipid droplet was shown in the cytoplasm, shorter cellular processes which was characteristics of chondrocyte were well developed. The membrane bounded or membrane unbounded electron dense material was scattered in the extracellular matrix. The cells showed degenerative changes, ie rupture of the cell membrane, loss of cytoplasm, outflow of cell organelle were also observed. The cells in close vicinity to synovial cavity showed different aspects from chondroid cells. The cells were long, and had no cellular process, and their nucleus was long and occupied the most parts of the cytoplasm. So the organelle and intracytoplasmic microfilament were not well developed. The articular surface of the articular disc was lined with amorphous electron dense material.
비만 여성을 대상으로 한 해조류 추출물의 국부처치와 운동 병행을 통한 지방분해 효과의 임상적 검정
서태수,이인숙,변재철,김규호,박승한,김유영,김상국,김현정,김상기,신동철,성미영,박종석,김종기,박언휘 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.3
This study was carried out to test the body fat breakdown during topical seaweed extract treatment and in combination with the aerobic exercise. Of 20 female obesity subjects, ten treated a cream types of seaweed extract(control group) and ten treated a placebo cream(placebo group). Topical treatment of each sample was designed for 4 weeks, and treated with 2 times a day about 10ml on the abdomen and the thigh, respectively. All subjects were participated aerobic dance half hour a day and four times a week. After 4 weeks of topical treatment and exercise, they were measured body fat, skinfold thickness, body circumference. Then venous blood samples were taken and analyzed blood cells, serum enzymes, and serum lipids. There were reduction of body weight, % of body fat, antebrachium, brachium, chest, waist, thigh circumference and abdomen thickness between pre and post treatment in both group. Especially, body weight and % of body fat significantly reduced in the subjects treating the seaweed extract than those treating the placebo. There were significant decrement in total cholesterol and LDL whereas increment in HDL and TBIL in the control group, but no significant difference in the placebo group. In conclusion, there were 7.6% of body fat reduction during 4 weeks of aerobic exercise and 21% of body fat reducement in combination of topical seaweed treatment and exercise, respectively. These results suggest that the topical treatment of seaweed extract should be very effective for fat breakdown. Especially, the body fat breakdown maximized in the combination with the topical seaweed extracts treatment and the aerobic exercise.
김성국,이영두,이승엽,최용환,박언휘,탁원영,조창민,배한익,염현규,권영오,정준모 대한간학회 2000 Clinical and Molecular Hepatology(대한간학회지) Vol.6 No.4
Focal nodular hyperplasia (FNH) is a rare benign hepatic tumor occurring predominantly in women of childbearing age. Generally oral contraceptive is not associated with FNH but might accentuate the vascular abnormalities which may cause the lesion to enlarge and, very rarely, to rupture. FNH is typically asymptomatic and seldom bleeds. Often it is incidentally observed during imaging procedures performed for some other reasons. The histologic feature of FNH is characterized by areas of localized growth of mature hepatocytes and septal fibrosis. Surgical resection is seldom required because of the benign nature of the lesion and its lack of significant complication. We experienced a case of focal nodular hyperplasia without liver cirrhosis confirmed by surgical resection and histologic examination. in a 47-year-old man..(Korea J Hepatol 2000;6:524-529).