생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

일반논문 : 일실된 『姜氏甘藷譜』에 대하여 -자료소개 및 그 실학사적 의의-

( Soo Kyung Oh ) 한국실학학회 2013 한국실학연구 Vol.0 No.26

『강씨감저보』는 현재 그 실물이 일실(逸失)된 것으로 전해진다. 그러나 이 책은 일실된 것이 아니고, 연경재 성해응(成海應; 1760~1839)이 지은 『연경재서종(硏經齋書種)』이라는 책 뒤에 『감저보(甘藷譜)』라는 이름으로 부록되어 현전(現傳)한다. 그러므로 이 책은 독립된 한 권의 책으로 존재하는 것이 아니라, 부록으로 전해진 셈이다.『감저보』는 강필교(姜必敎; 1722~1798)가 편집(編輯)한 것으로 되어 있다. 강필교는 동래부사를 지낸 강필리(姜必履; 1713~1767)의 친동생이다. 동생 강필교는 형님 강필리가 생전에 일본사람들에서 구입한 『감저종식방』을 참고하여 만든 『저보(藷譜)』라는 책을 저본(底本)삼아, 『감저보』라는 새로운 책을 편찬한 바, 이것을 후대 사람들이 강씨형제가 공동으로 지은 책이라는 뜻으로 『강씨감저보』라고 일컬은 것으로 짐작된다. 강필리가 지은 『저보』라는 책이 현존하지 않아 단언하기는 어렵지만, 동생 강필교의 『감저보』를 통해 이 책의 내용은 대략 짐작할 수 있는 바, 이 책은 고안고(故案考), 속록고(續錄考), 기문고(記聞考), 험시고(驗試考), 부록언해(附錄諺解) 등으로 나누어져 있다. 동생 강필교는 형님 강필리의 이 『저보』를 중심으로 여기에다 자신의 감저종식방법을 보완하여 좀 더 완벽한 감저[고구마] 재배방법을 기술하고자 하였던 바, 그는 이 『감저보』를 고실고(故實考), 속록고, 기문고, 험시고, 부록언해 등으로 체재를 나누어 설명하였다. ‘고안고’를 ‘고실고’로 바꾼 것을 제외하고는 강필리의 『저보』와 꼭 같은 이름으로 그 편차가 이루어져 있는 셈이다. 선종한(宣宗漢)·김장순(金長淳) 공저의 『감저신보(甘藷新譜: 일명 『金氏甘藷譜』)』는 강필리의 『저보』에 나온 감저종식방법이 오류가 많아 이를 바로잡기 위해 새로운 감저재배서를 만들었다고 한 바 있다. 실제로 『김씨감저보』는 「강씨감저보변와(姜氏甘藷譜辯訛)」라는 항목을 두어 강필리가 지은 『저보』의 잘못된 점을 하나하나 지적하고 있는 바, 선종한과 김장순은 이 『저보』를 『강씨감저보』라고 생각한 것이다. 그러나 이는 일본사람들이 전해준 감저종식방을 토대로 만든 강필리의 『저보』일 뿐, 강필교가 다시 수정보완한 『감저보』(일명 『강씨감저보』)를 말한 것은 아닌 것으로 생각된다. 따라서 오늘날 우리들이 일실된 것으로 알고 있는 『강씨감저보』는 바로 강필교가 작고한 형님의 『저보』를 저본삼아 다시 수정보완한 『감저보』를 말하는 것으로 보아야 한다. 결국 『강씨감저보』는 강필리·강필교 형제의 공동저서인 셈이다. 『강씨감저보』는 우리나라 고구마 재배서의 원조인 점에서 무엇보다 중요하게 거론되어져야 한다. 이 책의 뒤를 이어 만들어진 모든 감저재배서, 즉 서호수(徐浩修)의 『감저조(甘藷條)』(1799), 김장순·선종한의 『김씨감저보(감저신보)』(1813), 서경창(徐慶昌)의 『종저방(種藷方)』(1813)과 『감저경장설(甘藷耕藏說)』(1813), 조성묵(趙性默)의 『종감저법(種甘藷法)』(1832), 서유구(徐有구)의 『종저보(種藷譜)』(1834)들이 모두 이 『강씨감저보』의 재배경험을 기반하여 만들어진 것인 바, 우리의 농학사에서 차지하는 이 책의 의의는 그 어떤 저서보다 중요하다고 생각된다. 『Kang`s Sweet Potato Book(姜氏甘藷譜)』 is told to this day that the genuine article is lost. However, the book is not lost. It is in existence as the appendix, titled 『Sweet Potato Book(甘藷譜)』, to the 『Yeonkyungjae Seojong(硏經齋書種)』 written by Yeonkyungjae Seong, Hae-Eung(成海應; 1760~1839). Therefore this book is not an independent book, but has been passed down as an appendix. 『Sweet Potato Book(甘藷譜)』 is said to be edited by Kang, Pill-Kyo(姜必敎; 1722~1798). Kang, Pill-Kyo is a brother of Kang, Pill-Lee(姜必履; 1713~1767), who served as the ward head of Dong-nae. Kang, Pill-Lee wrote 『Potato Book (藷譜)』 using 『Gamjeojongsikbang(甘藷種植方)』 he had bought from Japanese. And his younger brother Kang, Pill-Kyo wrote the new book 『Sweet Potato Book(甘藷譜)』 based on his older brother`s. It is thought to be called 『Kang`s Sweet Potato Book(姜氏甘藷譜)』 by future generations meaning that the Kang brothers are the co-authors. Because 『Potato Book (藷譜)』 is not in existence we cannot assure though, guessing from the younger brother Kang, Pill-Kyo`s 『Sweet Potato Book(甘藷譜),』 『Potato Book (藷譜)』 is consisted of 「Goango(故案考)」, 「Soklocgo(續錄考)」, 「Kimungo(記聞考)」, 「Humsigo(驗試考)」 and 「Bulockunhae (附錄諺解)」. Kang, Pill-Kyo pursued to supplement his older brother`s 『Potato Book (藷譜)』 with his own seeds gathering method. So he systematized 『Sweet Potato Book(甘藷譜)』 as 「Kosilgo(故實考)」, 「Soklocgo」, 「Kimungo」, 「Humsigo」 and 「Bulockunhae」. The contents and their order are the same as Kang, Pill-Lee`s 『Potato Book (藷譜)』 except switching 「Goango(故案考)」 with 「Kosilgo(故實考)」. Seon, Jong-Han(宣宗漢) and Kim, Jang-Soon(金長淳) co-wrote 『Sweet Potato New Book(甘藷新譜: so-called 『Kim`s Sweet Potato Book(金氏甘藷譜)』 and it aimed to correct the mistakes in sweet potato seeds gathering method presented in Kang, Pill-Lee`s 『Potato Book (藷譜)』. 『Kim`s Sweet Potato Book(金氏甘藷譜)』 has an item titled 「Kang`s Sweet Potato Book Corrections(姜氏甘藷譜辯訛)」 and points out mistakes one by one from Kang, Pill-Lee`s 『Potato Book (藷譜)』. Seon, Jong-Han(宣宗漢) and Kim, Jang-Soon(金長淳) seem to have thought this 『Potato Book (藷譜)』 as 『Kang`s Sweet Potato Book(姜氏甘藷譜)』. However, it was only Kang, Pill-Lee`s 『Potato Book (藷譜)』, which was based on the 『Gamjeojongsikbang』 he had bought from Japanese, not the 『Sweet Potato Book(甘藷譜)』(so-called 『Kang’s Sweet Potato Book(姜氏甘藷譜)』). Hence, 『Kang`s Sweet Potato Book(姜氏甘藷譜)』, which is thought to be lost, seems to be Kang, Pill-Kyo`s 『Sweet Potato Book(甘藷譜)』 which was corrected and complemented from his older brother`s 『Potato Book (藷譜)』. Consequently, Kang, Pill-Lee and Kang, Pill-Kyo bothers are the co-authors of 『Kang`s Sweet Potato Book(姜氏甘藷譜)』. 『Kang`s Sweet Potato Book(姜氏甘藷譜)』 is very important as the originator of sweet potato book in Korea. All following sweet potato books such as Seo, Ho-Soo(徐浩修)`s 『Sweet Potato Article(甘藷條)』(1799), Seon, Jong-Han(宣宗漢) and Kim, Jang-Soon(金長淳)`s 『Kim`s Sweet Potato Book(金氏甘藷譜)』(1813), Seo, Kyung-Chang`s(徐慶昌) 『Jonggebang (種藷方)』(1813), 『Kamgekyunggjangsul(甘藷耕藏說)』(1813), Cho, Seong- Mook(趙性默)`s 『Jongkamgepub(種甘藷法)』, Seo, Yoo-Gu(徐有구)`s 『Seed Potato Book(種藷譜)』(1834) are based on the growing experience from this Kang`s 『Sweet Potato Book(姜氏甘藷譜)』. The significance of this book in Korean agricultural history is foremost among other books.

Properties of inverse nonpremixed pure O₂/CH₄ coflow flames in a model combustor

Choi, Sun,Kim, Tae Young,Kim, Hee Kyung,Koo, Jaye,Kim, Jeong Soo,Kwon, Oh Chae Elsevier 2015 ENERGY Vol.93 No.1

<P><B>Abstract</B></P> <P>In order to evaluate the potential of oxy-combustion of inverse nonpremixed coflow flames using a clean fuel to improve the combustion and emission characteristics compared with the normal coflow flames using air, the fundamental properties of inverse nonpremixed pure oxygen (O<SUB>2</SUB>)/methane (CH<SUB>4</SUB>) coflow flames in a model combustor are experimentally investigated. The combustion stability (extinction) limits, structure and nitrogen oxide (NO<SUB>x</SUB>) emissions of the inverse coflow flames in the quartz-windowed combustion chamber with a single shear coaxial injector and a fully opened exhaust nozzle are measured using OH<SUP>∗</SUP> chemiluminescence, a schlieren imaging system and a gas analyzer. Results show four distinct stability regimes and limits: the attached flame regime, the near-blowout flame regime, blowout limits and blowoff limits. The direct flame and OH<SUP>∗</SUP> chemiluminescence images confirm the cooling effects of chamber walls that cause no steady liftoff flame and only the near-blowout flame. The extinction limits of the inverse CH<SUB>4</SUB>/O<SUB>2</SUB> coflow flames are extended compared with the normal CH<SUB>4</SUB>/air coflow flames and reasonable levels of NO<SUB>x</SUB> emissions are observed, which supports that oxy-combustion of the inverse nonpremixed coflow flames using the clean fuel is acceptable for practical application, providing a useful database for modeling the flames in the confined chamber.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Extended stability limits of inverse nonpremixed O<SUB>2</SUB>/CH<SUB>4</SUB> coflow flames are found. </LI> <LI> Stable, attached flames and oscillating, near-blowout flames are observed. </LI> <LI> Direct flame and OH<SUP>∗</SUP> chemiluminescence images indicate no steady liftoff flame. </LI> <LI> Reasonable levels of NO<SUB>x</SUB> emissions are observed. </LI> <LI> Inverse nonpremixed O<SUB>2</SUB>/CH<SUB>4</SUB> coflow flames are acceptable for practical applications. </LI> </UL> </P>

오징어젓 숙성 중 N-nitrosamine 생성에 관한 연구

오창경,김수현,송대진,오명철,김성수 濟州大學校工科大學産業技術硏究所 1996 尖端技術硏究所論文集 Vol.7 No.1

본 연구는 오징어에 식염 20%를 첨가하는 고식염구와 식염 10%에 솔비톨, 에탄올, 젖산 등을 첨가한 저식염구로 나누어 오징어젓을 제조한 후, 이들을 숙성시키면서 N-nitrosamine의 생성 여부와 숙성 중 그의 전구물질인 아질산염과 질산염, DMA 및 TMA와 TMAO의 변화를 검토하였다. 오징어젓 숙성 중 pH는 저식염구가 5.79∼6.07, 고식염구가 6.05∼6.21 사이에서 변화하였다. 아질산염질소와 질산염질소는 각각 0.75∼1.04mg/kg 및 1.22∼1.42mg/kg 사이에서 변화하였다. TMA-N는 숙성 중 계속하여 증가하여 숙성 140일에는 고식염구가 20.1mg%, 저식염구가 14.9mg%였으며, TMAO-N는 생시료에서 27.9mg%이었던 것이 숙성 중기까지 완만하게 증가하다가 그 이후에는 다시 감소하였다. DMA-N는 숙성 중 계속하여 증가하였으며 숙성 140일에는 고식염구는 19.7mg/kr, 저식염구는 13.3mg/kg이었다. 오징어젓의 N-nitrosamine으로는 NDMA만이 검출되었고, 숙성 40일째에 저식염구에 흔적량, 고식염구에 3.8㎍/kg의 양으로 생성되었으며 숙성 중 계속해서 증가하여 57.5∼74.7㎍/kg에 이르렀다. Possibility of formation of carcinogenic N-nitrosamines and changes in their precursors during the fermentation of high salt-fermented squeid, Sepiell maindroni, group with 20% of salt and low salt-fermented squid group with 10% of salt, 6% of ethanol, 6% of sorbitol and 0.5% of lactic acid were investigated. pH during the fermentation of squid changed in the ranges of 5.79∼6.07 and 6.05∼6.21 in low and high salt group, respectively. The contents of nitrite- and nitrate-N during the fermentation were in the ranges 0.75∼1.04 and 1.22∼1.42mg/kg, respectively. Trimethylamine(TMA) was continuously increased during the fermentation and showed 20.1 and 14.9mg% in high and low salt group after 140 days of the fermentation, respectively, while trimethylamine oxide(TMAO) was gradually increased from the beginning until the middle period of fermentation and then was again decreased. Dimethylamine(DMA) was continuously increased during the fermentation and showed 19.7 and 13.3mg/kg in high and low salt group after 140 days of the fermentation, respectively. N-nitrosamines in salt-fermented squid was detected only N-nitrosodimethylamine(NDMA), which its amount was trace in low salt group and 3.8㎍/kg in high salt group on 40 days of the fermentation and then continuously increased in the ranges of 57.5∼74.7㎍/kg in both groups during the fermentation.

골수강내 금속정을 이용한 경골 근위간부 골절의 치료 : AO 골절형과 Poller 나사 사용 유무에 따른 분석 Analysis According to AO Classification and the Poller Screw

오창욱,김성중,전인호,경희수,박병철,인주철,김경훈,우연기 대한골절학회 2004 대한골절학회지 Vol.17 No.2

제대혈 단핵구 냉동보존 기법에 따른 해동 후 세포 회복률 비교 : 급속 냉각 vs 통제속도 냉각 Dump Freezing vs Controlled Rate Freezing

오상철,김병수,윤수영,박경화,윤소영,김석진,서재홍,최철원,신상원,김열홍,김준석 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

연구배경: 저자 등은 조혈모세포를 -80℃ 냉동고에서 급속냉각법을 통하여 경제적으로 보존하는 방법의 냉동보존 효율이 통제속도냉각 후 액체질소보존법의 냉동보존 효율과 동등한 유효기간을 규명하고자 본 연구를 시행하게 되었다. 방법: 총 20 명의 산모에서 채집한 재대혈을 대상으로 하여 10% DMSO로 제대혈을 -80℃로 급속냉각한 경우(실험군)와 통제속도냉각장치로 냉각 후 액체질소냉동고에서 보존한 경우(대조군)에서의 해동 후 세포 회복률 및 기능의 회복 정도가 동등한 기간의 범위를 파악하기 위하여 3달 간격으로 총 18개월 동안 각 군 간의 해동 후 단핵세포회복률, CFU-GM 집락회복률, CD34+ 세포회복률 등을(평균)±(표준편차)로 표시하여 Paired t test를 통하여 비교, 분석하였다. 결과: 1) 생존 단핵세포 회복률: 대조군(통제속도냉각군)과 실험군(급속냉각군)을 비교하여 보았을 때, 모든 기간 동안 각 군 간에 유의한 차이를 발견할 수 없었다. 2) CFU-GM 집락 회복률: 대조군(통제속도냉각군)과 실험군(급속냉각군)을 비교하여 보았을 때 12개월 이후부터는 대조군이 실험군에 비하여 높은 경향의 회복률을 보였으나 양 군 간의 유의한 차이는 없었다. 3) CD34+세포 회복률: 대조군(통제속도 냉각군)과 실험군(급속 냉각군)을 비교하여 보았을 때 12개월 이후부터는 대조군이 실험군에 비하여 높은 경향의 회복률을 보였으나 양 군 간의 유의한 차이는 없었다. 결론: 제대혈에서 채집된 단핵구를 냉동보존 시 18개월까지 -80℃ 급속 냉각법으로 보존한 군과 통제속도 냉각 후 액체질소 보존 군 간에 단핵세포 회복률, CD34 양성 세포 회복률, CFU-GM 세포 회복률의 유의한 차이가 없었음을 관찰할 수 있었다. Background: This study was designed to compare the mononuclear cell recovery rate of cord blood after thawing and freezing according to cryopreservation methods: dump freezing vs controlled rate freezing. Methods: Twenty samples of cord blood were divided into dump freezing and controlled rate freezing groups. The viable mononuclear cell, CFU-GM and CD34+ cell recovery rates were evaluated at the time of post-thawing 1, 3, 6, 9, 12, 15 and 18 months after cryopreservation with 10% DMSO and compared between two groups. Results: The mean viable mononuclear cell recovery rates from cryopreservation 1 month to 18 months of dump freezing and controlled rate freezing were 81~88% and 82~89%, respectively. There was no statistical difference between two groups. The mean CFU-GM colony and CD34+cell recovery rates of dump freezing and controlled rate freezing (cryopreservation duration: 1 month to 18 months) were 66~76% vs 71~78% and 86~95% vs 86~94%, respectively. There was no statistical difference between the results of two groups. Conclusion: The -80℃ storage without rate-controlled freezing of cord blood was practically applicable with satisfactory cell recovery rates after thawing, which was comparable with those cryopreserved with controlled rated freezing and liquid nitrogen storage.

김치의 N-Nitrosamine 오염

오창경,오명철,송대진,김수현,Hotkchiss, J. H. 濟州大學校工科大學産業技術硏究所 1999 尖端技術硏究所論文集 Vol.10 No.1

김치 발효중 Ν-nitrosamine의 함량 변화 및 오염 출처에 대한 연구를 수행한 결과, 식품에서 흔히 발견되는 NDMA와 NDEA가 검출되지 않은 반면, 13종의 미확인 Ν-nitrosamine이 검출되었으며, 이들은 모두 Ν-nitrosamines으로 동정되었다. 이들은 주로 배추와 일부 멸치 젓갈에서 유래된 것이었다. 김치 발효 초기에는 배추에서 유래된 Ν-nitrosamine들이 감소되었으며, 발효 말기에는 일부 Ν-nitrosamine이 미량 증가되었다. Changes in Ν-nitrosamines during Kimchi fermentation were investigated. Thirteen unknown Ν-nitrosamines were detected during Kimchi fermentation, but Ν-nitrosaminethylamine(NDMA) and Ν-nitrosaminethylamine(NDEA) were not. Most of these Ν-nitrosamines were originated from chinese cabbage and some of from fermented anchovy sauce. The content of Ν-nitrosamines decreased remarkably at the initial stage of fermentation, but increased slightly at the final stage.

영지버섯 생장점 단백다당체 GLB의 대식세포 활성화 효과

오정연,조경주,정수현,김진향,Lillehoj, H.S.,정경수 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

In the previous study we described the antitumor activity of GLB. a protein-polysaccharide fraction of the growing tips of Ganoderma lucidum, against sarcoma 180 solid tumor in ICR mice. In this study we investigated the stimulatory activity of GLB on macrophages. When analyzed using a flow cytometer. GLB (100 ㎍/㎖) was found to increase the phagocytic activity of the BALB/c mouse peritoneal macrophages as well as chicken macrophage BM2CL cells against FTTC-labeled C. albicans by 55.2% and 21.2%, respectively. GLB also increased the spreading and the expression of MHC class Ⅱ molecules of BM2CL cells as well as the mouse peritoneal macrophages. From these results, it is clear that GLB is a strong stimulator to the macrophages.

환기시스템의 효율 향상을 위한 후드 내 유해물질 포착속도 개선에 관한 연구

오율권,김영선,박경수 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.1

Most of the hood maximize on capture velocity concentrate in inhalation capacity of harmful materials on hood of center. But, decrease generally ventilation system efficiency because, the edge of hood decrease capture velocity of harmful materials. So, development on the gas-guidance-device for increase of capture velocity of harmful materials in hood increase generally ventilation system efficiency. In this study, flow in hood doing visibility after numerical analysis using the Phoenics 3.1 on commercial code of heat and fluid using finite volume method. And, boundary conditions establish the same condition as work space that it is temperature is 20 degrees, pressure in 1 atm, velocity in duct is 6.85m/s, diameter is 400mm. Also, gas-guidance-device draw optimum shape as a result comparison and analysis as being numerical analysis of variety shape. As the result of study, compare hood have gas-guidance-device with hood have no gas-guidance-device identify increase on harmful materials capture velocity. Also, gas-guidance-device identify best inhalation efficiency in hood when it is 125mm wide and a ratio of 4 to 6 on side. So, safety of worker and work environment improvement expect helped as a result remove effective on harmful materials in work space and cut down on expenses of occurrence when the inhalation volume again design for efficiency improvement of ventilation system.

Android SDK를 이용한 2D RPG 모바일 게임 제작

오경훈, 안성옥, 김수균 배재대학교 공학연구소 2012 공학논문집 Vol.14 No.1

Mobile game Soul Chaser was manufactured using the Android SDK. The game uses 2D graphics and RPG genre. Neat graphics and simple, easy-operation can be enjoyed by anyone, Based on the scenario with the completed structure, Causing the player's interest through a variety of events foreshadowing. Simple interface complex operation is difficult because of the nature of the mobile games, Story of conversational RPG game genre is very appropriate, because the With the development of the mobile gaming market will be even more prominence. KEY WORDS:Mobile game,Android SDK.