Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

정신분열병 환자들의 自己灣入에 관한 예비 연구

박성봉,김영미,전성일,이기철,김영훈,정영조,이영렬,이정호,최영민 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.6

목 적 : 정 신분열병 환자의 자기만입 정도에 영향을 미치는 인구학적 변인들 및 질병연관 변인들을 규명해 보고자 하였다. 또한. 환자들의 자기만입과 병식 사이의 연관성. 자기만입과 자존심 사이의 연관성을 알아보고자 하였다. 방 법 : 정신과 의사가 병록지와 면담을 통하여 대상 정신분열병 환자들의 인구학적 변인들 및 질병연관 변인들에 관한 자료를 얻고 환자들의 병식 정도를 평가한 후, 환자들로 하여금 자기보고형의 자기만입 척도와 자존심 척도를 시행하게 하였다. 총 111명의 환자가 연구대상으로 선택되었다. 결과: . 1) 여자가 남자보다 유의하게 높은 자기만입 척도 점수를 나타내었다 2) 질병연관 변인들 중 하위집단 사이에 자기만입 척도 점수의 유의한 차이를 보이는 변은 없었다. 3) 자기만입 척도 점수와 병식 점수 사이에 유의한 상관관계가 없었다. 4) 자기만입 척도 점수와 자존심 척도 점수 사이에 유의한 역상관 관계를 보였다. 결 론 : 본 예비 연구의 결과로 보아 정신분열 병 환자의 병식이 부족한 상태에서도 자기만입이 진행될 수 있으며. 자기만입이 심할수록 환자의 자존심이 저하되는 것으로 생각된다. 또한 여자 환자들이 남자 환자들보다 자기만입에 더 취약한 것으로 생각된다. 따라서 정신분열 병 환자의 이해와 치료를 위하여 자기만입에 관한 지속적인 연구가 필요할 것으로 생각된다. objective : This study was conducted to identify demographic variables and illness related variables which may affect the severity of self-engulfment in the patients with schizophrenia. We also studied the relationship between self-engulfment and insight, and the relationship between self-engulfment and self-esteem. Methods : Data on demographic variables and illness related variables for the subjects were gathered from hospital records and clinical interviews by the psychiatrists. Degree of insight for the subjects was assessed through clinical interviews by the psychiatrists. Degree of self-engulfment and self-esteem for the subjects was assessed from the self-engulfment scale and the self-esteem scale respectively. A total of 111 patients with schizophrenia were selected for statistical analysis. Results 1) The females exhibited significantly higher scores than the males on the self-engulfment scale. 2) There was no illness related variable, which exhibited a significant difference among subgroups on the self-engulfment scale. 3) There was no significant correlation between the self-engulfment scores and the insight 4) There was a significantly inverse correlation between the self-engulfment scores and the self-esteem scores. Conclusion : The results of this preliminary study suggest that self-engulfment may develop despite lack of insight and that there was inverse relationship between self-engulfment and self-esteem in the patients with schizophrenia. It also suggests that females are more subject to self-engulfment than males. So, authors suggest that it is valuable to do further studies of self-engulfment in the patients with schizophrenia.

백서 간재생중 H2B Histone 유전자 발현에 대한 Octamer Binding Transcription Factor의 역할

임규,방재웅,최두식,손미영,이영철,박청,김태동,김영래,박종일,윤완희,박승길,황병두 한국노화학회 2001 한국노화학회지 Vol.11 No.1

종합병원 간호사의 밤번 근무에 영향을 미치는 요인에 관한 연구

박혜자,유인영,이영미,정문희 한국보건통계학회 2003 보건정보통계학회지 Vol.28 No.1

This study is conducted to find influencing factors on unrse's night shift works. From 20th July to 20th August, the survey was performed in a general hospital located at Kyunggi-do area, by the use of questionnaire. Collected 204 data were analysed through SPSS/Win 11.0. Results are as follows: 1. It was shown that night shifting word periods in special ward is longer than that in general ward. Exactly, shifting periods of night nurse work is 28.7days, 16.7days in special and general ward, individually. But numbers of night nurse worker are 3.4 and 2.48 persons in special and general ward, individually. And there were more patients in general wards than that in special ward. Namely, 54.3 patients in general ward and 19.3 patients in special ward. And off duty days and overtime on average in general ward were more much than that in special ward. The preference about night nurse works, the general ward was higher than that in general wards. In case of avoiding the night nurse work, the reasons that were mental stress. excess of duty, the burdens on work by oneself in general wards were shown much more frequency than that in special ward. 2. Physical-mental, social conditon of nurse worker's health in special wards were better than that in general wards. The stress level of nurse workers in general ward was higher than that in special ward. The factors of the influence on the preference on social-mental conditions of health were number of off duty days on average per month, number of night nurse working, and income of all family. Therefore, theses results were shown that the stress of nurses in general ward was much more than that in special ward. And the preference on night nurse work in general ward was lower than that in special ward. Accordingly, It was shown that the policy for support were need. The nurse administrator have to prepare complementary systems in organized level, to develop the positive recognition about night nurse work. For example have to reduce the dissatisfaction with systematic improvement, in other words control of off duty bonus, reasonable overtime and night nurse work bonus, and night working periods, and with control nursing human power and policy for reduction of overstres according working conditions and characters of business in various types of ward.

동화를 통한 통합적 프로그램 적용을 통한 18~36개월 영아의 읽기 능력신장을 위한 시안

박해미,장영미 기전여자대학 2002 論文集 Vol.22 No.-

The purpose of this study is to formulate the basic outline to enhance reading ability of 18-36 month toddlers based on the whole language program through story-telling books. The study was proceeded by selecting story-telling books, classifying the books into the main idea, and researching whole language program for each story-telling book . The research questions are as follows: First, the story-telling book is selected for 18-36 month toddlers. Second, the story-telling book is classified into the main idea, and materials are produced. Third, the whole language program through story-telling books is organized. This study is significant in terms of formulating the basic outline to build up the integrated curriculum for 18-36 month toddlers based on the theory that literature and reading ability of toddlers are strongly related. Therefore, the application of integrated curriculum through story-telling books for 18-36 months toddler would be suggested as the next study.

환자, 간호사, 의사가 지각하는 교육요구도와 교육제공정도에 관한 연구

박인숙,문영숙,조미경,최은숙,이경숙,정길수 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

The purpose of this study was to provide the basic data for the planning of education of patients by exploring and comparing patients', nurses' and doctors' perception of the educational needs and degree of providing education. 66 hospitalized patients undergoing chemotherapy and 44 nurses, 42 doctors assigned for the direct care were sampled in 1 university hospital in Taejon from August 30, 1993 to September 30, 1993. For the comparing patients', nurses' and doctors' perecption of the educational needs and degree of providing education, the researcher used Kims' Educational need scale which is composed of 20 item Likert type 5 point scale. Data were analyzed by means of percentile score, SPSS, t-test, ANOVA, Pearson correlation. The result were as follows 1. Comparision of total Mean score patients', nurses' and doctors' perception of the educational need ordering nurses(M=4.0465), patients (M=3.9803), doctors (M=3.8309). The difference between the three group's total Mean score is showed no statistical significant difference. 2. Comparision of total Mean score patients' educational need, patients' percetion of nurses' and doctors' degree of providing education, patients' educational need is scored 3.9803, patients' perception of nurse is scored 2.3431, patients' perception of doctor is scored 2.5022. The difference between the patients' educational need and medical teams' degree of providing education is showed statistical significant difference(P<.001). 3. Comparision of total mean score nurses' educational need is scored 4.0465, nurses' perception of nurses' degree of providing education is scored 2.9920, patients' perception of nurses' degree of providing education is scored 2.3431. The difference of between the nurses' educational need and nurses' perception of nurses' degree of providing education is showed statistical significant difference(P<.001) 4. Comparision of total mean score doctors' educational need is scored 3.8309, doctors' perception of doctors' degree of providing eduation is scored 3.1726, patients' perception of doctors' degree of providing education is scored 2.5022. The difference of between the doctors' educational need and doctors' perception of doctors' degree of providing education is showed statistical difference(P<.001).

혈당측정기 GlucoDr™ System의 평가

박철영,류미숙,우정택,김성운,김진우,김영설,안규정 대한당뇨병학회 2002 임상당뇨병 Vol.3 No.2

연구배경: 당뇨병 환자에서 엄격한 혈당조절은 환자의 예후와 밀접한 관련이 있다. 혈당조절을 철저히 하기 위해서는 자가 혈당 측정기의 사용이 필수적이라 할 수 있다. 또한 저혈당이 빈번하게 발생하거나, 위험요소가 있는 환자들에게도 유용하게 사용될 수 있다. 이에 저자들은 전기화학감지법의 원리를 이용하여 국내에서 처음 생산된 자가혈당측정기인 GlucoDr™ blood Glucose Testing System(ALL Medicus, Co, Korea)를 현재 국내에서 시판되고 있는 다른 제품과 비교, 평가하여 임상적 유용성을 살펴보고자 하였다 방법: 혈당측정기준장비는 YSI 2300 STAT Plus(YSI Incorporated, USA)를 사용하였으며 GlucoDr™외에 Glucocard(KDK, Japan), Precision QID(Abbott Laboratories, Co, USA), Glucotrend(Roche, USA), Surestep(Lifescan, USA)의 다른 혈당측정기를 같이 평가하였다. 정밀도, 직선성, 비교방법과의 상관관계 평가, 검체량에 따른 영향, 헤마토크리트에 따른 영향, 검사자에 따른 영향, 항응고제에 따른 영향들을 평가하였다. 결과: 본 연구에서의 주된 평가대상인 GlucoDr™의 평가결과, 정밀도를 나타내는 검사 내, 검사 간 변이계수는 8%이내였으며, 45~500mg/dL 범위에서 R²=0.9906, 0.9984 정도의 직선성을 나타내었다. 본 연구에 있어 비교방법으로 이용한 YSI 2300 STAT Plus와의 상관관계 및 정확도는 y = 1.0058x + 0.453, R = 0.9853인 것으로 나타났다. 검체량이나 검사자 및 항응고제에 따른 측정값에는 유의할만한 차이가 없는 것으로 나타났으나, 헤마토크리트가 증가 또는 감소함에 따라 최소 10%에서 최대 26% 가량의 영향을 받는 것으로 관찰되었다. 결론: GlucoDrTM는 국내 사용되는 다른 혈당측정기와 비교하여 유용할 것으로 생각된다. Background: Self-monitoing blood glucose devices are sidely used in monitoring and point-of-care testing for the management of diabetic patients. We performed the present study to evaluate the performance of the GlucoDr™ blood glucose testing system using an electrochemical technique. Method: The GlucoDr™ was evaluated for linearity, precision, comparison of method, the effect of sample volume, hematocrit concentration, reapplication, operator and application methods. Results: The GlucoDr™ showed good linearity for glucose concentrations ranging from 52mg/dl to 475mg/dl(r²=0.971). The single day, and day-to-day, CV were within 8%. Excellent correlation was found between the GlucoDr™ and YSI 2300 STAT Plus(y=1.0058x + 0.453, r²=0.9710). The sample volume, reapplication, operator and application method produced no significant effect on the test result. An overestimation in the glucose values was found with low hematocrit concentrations. There was no significant effect by the anticoagulants, with the exception of citrate. Conclusion: The GlucoDr™ showed good linearity, precision and correlation with the reference method and provided rapid and reliable result for blood glucose levels. Therefore, the GlucoDr™ seems appropriate for clinical use in the management of diabetic patients.

의문사구 이동을 통하여 본 스페인어와 한국어의 차이에 관한 고찰

박영미 한국동서정신과학회 2002 동서정신과학 Vol.5 No.2

지구상에서 사용되는 자연어는 몇 가지나 될까? 보편문법 (Universal Grammar)이 추구하는 것은 다양한 인종, 종족, 민족만큼이나 다양한 인간의 자연어들 사이에 존재하는 언어보편적인 특징을 찾는 것이라 하겠다. 언어의 보편성을 가정함은 외현적으로 나타나는 수많은 차이에도 불구하고 인간이 사용하는 언어는 그 생성에 있어서 다르지 않고 문법에서 정하는 보편 원리에 따라 설명될 수 있음을 전제로 한다. 본고에서는 서양어와 동양어로 대표되는 스페인어아 한국어의 의문사 사용을 통하여 이들 두 언어간의 차이점을 살펴보고 이 차이점이 보편문법 체계하에서 어떻게 통합 설명되는지 알아보고자 한다. 본고의 전개는 다음과 같은 방식으로 이루어 진다. 제 1장에서는 우리가 자료 분석을 위해 이론적 배경으로 삼을 보편문법의 문법체계와 성립 배경에 대하여 알아보고자 한다. 제 2장에서는 초기 생성문법 (지배-결속이론)의 이론 내에서 의문사구 이동 규칙이 어떻게 적용되었는지를 살펴보며, 3장에서는 초기 규칙 체계로 설명되던 의문사구 이동을 생성문법의 최근 이론인 최수주의(Chomsky 1998,1999)내에서 재분석해보고자한다. 제4장에서는 2, 3장의 분석을 통한 결론을 도출하고자 한다. En este articulo vamos a comparar el movimiento del sintagma Qu- del espaiiol y del coreano cuyo proceso se difiere uno al otro. El espaiiol, inchso el mglk y el franc&, es una lengua que siempre tiene el sintagma Qu- en la posici6n inicial de la oracibn; en cambio, en el coreano el sintagma Qu- no se mueve en su posici6n de base, lo cud1 tambikn se observa en el chino y el japonts. Segh la GramLica Universal, esta aparente diferencia se puede explicar de manera unitaria si captamos el mecanismo del movimiento Qu- que ofrece su teoria. Para el anhlisis, tomaremos dos oraciones bkicas en las cuales el sintagma Qu- es el objeto del verbo: iQu6 compr6 Juan?; Chulsuga muutsl sanni?. Como es sabido, en las dos oraciones el objeto (qut y muut) se genera en la posicih del complement0 del verbo. si aceptamos que la oracion se proyecta hasta SComp (Chomsky 19811, el Espec del SComp ocupa el elemento Qu-, en este sentido el objeto Qu- del verbo comprar y sada debe rnoverse a la posicih del Espec del SComp. Pero, se puede preguntar porqut en coreano el sintagma Qu- no se mueve a esa posd6n. Planteada esta pregunta, revisaremos la regla del movimiento Qu- desde el sistema de Chomsky (1981) hasta la dltima teoria del minimismo (Chomsky 1998, 1999). Criticaremos el mecanismo de la gramitica en base de las reglas y adoparemos el sistema de Cotejo (Checking) o Acuerdo (Agree) de 10s rasgos no interpretables del minimismo para poder explicar adecuadamente. Mediante este nuevo sistema de Cotejo (Acuerdo) el ndcleo C tiene el rasgo no interpretable y debe ser eliminddo bajo el Acuerdo con el rasgo interpretable que tiene el elemento Qu- que se encuentra en la oracih. En este caso, C de las dos lenguas elimina s u rasgo no interpretable con el elemento Qu- de manera unitaria, y la lrnica diferencia del orden de palabras que se observa en las dos se debe al rasgo W E del C. Chomsky (1998, 1999) afirma que el PPE es un rasgo no interpretable que se satisface por el rnovimiento de una categoria. A diferencia del PPE del I que es obligatorio, el PPE del C y del v es optional, asi que la diferencia mostrada en las dos lenguas s61o se debe al rasgo PPE del C. El sisterna de Acuerdo tiene la ventaja de simplificar las cornplejidades operativas puesto que todas las operaciones sintkticas se reduce en este sistema eliminando asi tanto el rnovirniento explicito corno el implicito.

융모막 암종 성분의 간전이를 보인 S-결장의 복합 선암종-융모막 암종

오영하,이원미,박문향,이중달,김경숙 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.8

멀티미디어 성교육 프로그램이 남자 중학생의 성 태도와 성 지식에 미치는 효과

박효미,임미영 대한간호학회 아동간호학회 2003 Child Health Nursing Research Vol.9 No.2