Emulsion형 化粧品의 Escherichia coli 汚染防止에 대한 防腐制效果

柳美淑,金樟圭,元省皓,金男紀 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2

Most of cosmetics are emulsion products that contain the source of nutrition-vegetable oil, mineral oil and carbohydrate etc. -so there is much possibility of contamination by microbials. The purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by Escherichia coli but to sustain the quality and the wear of cosmetics as the result of finding optimum growth condition and the growth condition of Escherichia coli for enviro-factor, utilizing antiseptic so as to prevent contamination by observing it in emulsion type cosmetic. The results are as follows : 1. The optimum growth condition of Escherichia coli : pH7.0 and 37.5℃ The mixing volume ratio of water / oil was 70/30 in this experiment. 2. Adding antiseptics into emulsion type cosmetic, the number of Escherichia coli was decreased from 10^8(cell/ml) to 8 x 10^3(cell/ml) 3. Antibacterial effect against Escherichia coli was better as follows, p-hydroxy benzoic acid propyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid buthyl ester + acetic acid buffer solution > p-hydroxy benzoic methyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid methyl ester + p-hydroxy benzoic acid buthyl ester >p-hydroxy

성장호르몬분비성 뇌하수체 선종의 성장호르몬 방출호르몬 수용체 유전자의 발현과 내분비적 양상과의 관계

류미숙,양인명,박승준,우정택,김성운,김진우,김영설,최영길 경희대학교 생명자원과학연구원 1997 遺傳工學論文集 Vol.9 No.-

Growth hormone-releasing hormone (GHRH) plays a key role in the regulation of the proliferation and differentiation of somatomammotroph cells as well as secretion of GH. The actions of GHRH are mediated through the GHRH receptor (GHRH-R) that is a G protein coupled receptor with seven transmembrane domains. It has been demonstrated that alternative splicing occurs in the third cytoplasmic domain of rat and human GHRH-R mRNA. However, the clinical significance of the alternative splicing remains to be unsolved. To find an insight into the clinical significance, we investigate the correlation between the GHRH-R gene expression and a variety of clinical clinical and endocrinological findings in 11 acromegalic patients. Three different sized cDNA fragments 250 bp, 700 bp and 810 bp, were found after RT-PCR. The amount of 250 bp fragment was higher than those of the other two fragments. The clinical findings (age, size, GH level, frequency of paradoxical response to TRH or GnRH. octreotide response, hypothalamc somatostatinergic activity of the group with high expression of the 250 bp fragment did not significantly differ from those of the group with low expression. The GHRH-R gene expression of tumors with gsp oncogene did not significantly differ from that of tumors without gsp oncogene. These results suggest that the expression of GHRH-R gene may not be an important determinant for tumor growth, and the lower GH response to GHRH of tumors with gsp oncogene may not be attributed to the lower expression of GHRH-R gene. The expression of GHRH-R is likely to be regulated by a certain property of tumors for GH secretion and growth.

성장호르몬 분비성 뇌하수체 선종에서 소마토스타틴 수용체 (제2아형, 제5아형), G_i2α 및 Pit-1 유전자 발현

류미숙,양인명,박철영,우정택,김성운,김진우,김영설,최영길,김은희,박승준,김국기 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2

Background: Mutation of Gs protein subunit (gsp oncogene), detected in about 30∼40% of growth hormone (GH)-secreting pituitary tumors, is associated with an increased long-acting somatostatin analog octreotide sensitivity. However, the mRNA expression of somatostatin receptor (sst) was not changed in the GH-secreting pituitary tumor, regardless of whether they were gsp oncogene positive or negative. This suggests that the expression of genes coding for G_i2α, Pit-1 and the other factors involved in the regulation of secretory activity in somatotrophs is likely to be altered in gsp oncogene positive tumors. We observed the impact of the gsp oncogene on the expression of the genes coding for Gi2, Pit-1 and sst (2&5) in GH-secreting pituitary tumors. Methods: The GH response to octreotide was examined in 13 acromegalic patients before transsphenoidal adenomectomy. Genomic DNA and RNA were extracted from fresh frozen tumor tissues. PCR was performed to amplify and sequence the region between codon 184 and 251 that includes exons 8 and 9 of the Gs gene. Sst2, sst5, G_i2α and Pit-1 mRNA levels were measured by semi-quantitative RT-PCR. Results: Sst2 and sst5 mRNA transcripts were detected in all tumors (7 gsp +, 6 gsp-). The amount of sst transcripts varied considerably varied between the tumors. There were no significant differences in sex, age, tumor size, grade or basal GH levels. Pit-1 and sst2 mRNA levels were not different. In contrast, G_i2 mRNA levels were significantly higher in gsp (+) while sst5 mRNA levels were higher in gsp (-). Conclusion: These data suggests that gsp oncogene may increase Gi2α levels but decrease sst5 mRNA levels. However, Pit-1 and sst2 mRNA expression may not be affected by gsp oncogene. The increased expression of the G_i2α gene might be an inhibitory compensatory response to the action of gsp oncogene

방부살균제에 의한 유화형화장품에서의 Staplylococcus aureus 의 성장억제

류미숙,김장규,김남기,원성호 한국산업안전학회 1992 한국안전학회지 Vol.7 No.3

S. aureus를 유화형 화장품시료에 접종배양한 결과, 최적성장조건인 pH 7.0, 온도 37℃에서 시료조성에 따른 성장률은 물과 오일의 부피비 조성에서 70 : 30, 80 : 20, 90 : 10, 30 : 70, 50 : 50의 순서로 균증식이 되었다. 물성에 있어 화장품시료의 pH는 40일 경과시 7.6에서 6.5로 변화되어고, 상대적으로 낮은 굴절률을 가는 물질들이 영양원으로 소모되어 시료(sample 3)의 굴절율이 1.4415에서 1.4490으로 변화되었으며, 균의 증식으로 인하여 균일하게 유화된 유화형 화장품시료의 相이 파괴되었고 약간의 변색, 변취와 함께 크리밍형상, 응집현상이 나타났다. 방부살균제가 첨가되지 않은 경우 약20일 경과후 부터 제품의 변질이 시작되었으나 방부살균제를 첨가하여 challenge test를 실시한 결과, S. aureus의 성장이 억제되었으며 40일 경과시 균수는 방부살균제가 첨가되지 않은 시료의 경우 10^8개/㎖이었으나 p-hydroxy benzoic acid propy ester - phosphric acid buffer solution을 200ppm 첨가한 경우 7×10^8개/㎖로 균수가 현저히 감소하였다. 균의 성장억제 효과는 p-hydroxy benzoic acid propylester+phosphoric acid buffer solution〉p-hydroxy benzoie acid methyl ester+phospheric acid buffer solution〉p-hydroxy benzoic acid methyl ester+phydroxy benzoic acid propyl ester〉p-hydroxy benzoic acid methyl ester+acetic acid buffer solution의 순으로 우수하였고 pH, 농도, 균수 등은 양호한 수준을 유지하였다. Emulsion-type cometics contain many kinds of carbon and energy source i.e., vegetable oil, mineral oil and carbohydrate etc., those can be used as nutrients and caused contamination by micrabials. Thereby we have to keep cosmetics from the possibility of contamination by microbials. From this viewpoint, the purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by microbials but also to sustain the quality. In this experiment, we observed how many Staphylococcus aureus were grown in the prepared cosmetics with or without antiseptics so as to prevent contamination. When the contamination proceed, the stability of phase was disturbed and creaming phenomina was happened with some discoloration and bad smell. About 40 days after, the pH was changed from 7.6 to 6.5 and the refractive index of cosmetic raw materials were changed from 1.4415 to 1.4490(water : oil=70:30). By adding antiseptics into prepared cosmetics, the number of Staphylococcus aureus with challenge test method were decreased to 7×10^8 cell/㎖. For the antibacterial effect against Staphylococcus aureus, p-hydroxy benzoic acid propyl ester in phosphoric acid buffer solution was the best.

화장품(에멀젼형)에서 Pseudomonas aeruginosa의 성장과 방부살균제효과

류미숙(Mi Sook Ryu),김장규(Jang Kyu Kim),김남기(Nam Ki Kim) 한국생물공학회 1992 KSBB Journal Vol.7 No.2

휴대폰 중독군과 비중독군의 어머니의 양육태도와 의사소통 차이비교: 중학생을 대상으로

류미숙 ( Mee Sook You ),권미경 ( Mi Kyung Kwon ) 부모자녀건강학회 2011 부모자녀건강학회지 Vol.14 No.2

The purpose of this study is to compare the mother`s rearing attitude and communication between mobile phone addiction group and non-addiction group in middle school students. Methods: A cross-sectional descriptive study was performed with 574 students from 3 middle schools (1 boy school, 1 girl school, 1 coeducation school) at G-city in Gangwon Province. The data was collected from the 10th to 24th of July in 2010. Statistical analysis of the results was carried out by means, frequencies, chi-square, t-test and multiple regression with SPSS/WIN 18.0 program. Results: There were significant differences in mobile phone addiction group and non-addiction group in regard to sex, school year, grades, mother` age, purpose of mobile phone use, activity after school, phone bill per month. Also There were significant differences in mother`s rearing attitude and mother`s communication between two groups. Conclusion: Mobile phone addiction among students is not serious, however, possibility of becoming an addict still remains. This study suggests that we have to educate them about proper mobile phone use in advance to prevent them from becoming a mobile phone addict in early stage. Therefore, we need to develop an educational program to prevent mobile phone addiction.

성교육 프로그램이 청소년 성의식과 성태도에 미치는 효과 : 정신병원 청소년병동 입원환자를 대상으로

류미숙 외 국립서울병원 정신보건교육·연구센터 2008 정신건강정책포럼 Vol.2 No.1

• Purpose : Sexual Education Program on adolescence sexual health has been developed by researchers in accordance with the national education courses. However, there have been not the study which found the effects of this program on sexual consciousness and sexual attitudes of psychiatric in-patient adolescents. • Method : Non-equivalent control group pretest-posttest design was used. Twenty six adolescents participated in an experiment. National Seoul mental hospital in-patients were selected for a convenience sampling method and have been taught the sexual education program during six weeks. • Result : There were significant differences in the sexual consciousness(t:-2.835. p:0.009) and sexual attitudes(t:-1.826. p:0.008) after the sexual education program • Conclusion : Since the sexual education program can affirmative influence on the sexual consciousness and sexual attitudes of the adolescence, it is recommendable to perform the sexual education program suitable for every stage of growth and development.

타인에 의한 타당화 욕구, 눈치, 사회적 자기효능감이 청소년의 대인관계에 미치는 영향

류미숙(Ryu Mi Suk) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.13

본 연구는 청소년을 대상으로 타인에 의한 타당화 욕구, 눈치, 사회적 자기효능감이 대인관계에 미치는 영향과 심리적 기제를 알아보기 위한 것으로 타인에 의한 타당화 욕구가 대인관계에 미치는 영향에서 눈치와 사회적 자기효능감의 매개효과를 가정하였다. 이를 알아보기 위하여 경기도 소재 3개 중학교에 재학 중인 2, 3학년 학생 435명을 대상으로 2014년 12월부터 2015년 2월까지 설문조사를 실시하였다. 수집된 자료는 SPSS 21.0 프로그램을 사용하여 Cronbach’s α 계수 산출, 기술통계 분석, t-검증, 상관분석, 회귀분석을 실시하였으며 매개효과 검증을 위해 AMOS 21.0을 이용하여 구조방정식 모형 분석을 시행하였다. 연구 결과 본 연구에서 제안한 연구모형인 이중매개 모형이 대안모형에 비해서 수집된 자료에 가장 잘 부합하였다. 타인에 의한 타당화 욕구가 대인관계에 미치는 영향에서 눈치와 사회적 자기효능감이 순차적으로 매개하는 이중매개효과가 유의미했다. 또한 눈치가 대인관계에 미치는 영향에서는 사회적 자기 효능감이 부분매개 효과가 있었다. The purpose of this study was to investigate the effects of needs for approval, nunchi, social self-efficacy on adolescents interpersonal relationship and psychological mechanism. This study investigated mediating effects of nunchi, social self-efficacy on the relation between needs for approval and interpersonal relationship. The date for this study consist of 435 middle school students in Gyeonggido. To test the hypotheses, Needs of a Validation From Others Scale, Nunchi Scale, Social Self-efficacy Scale, Interpersonal Relationship Scale was used. Goodness of fit of the measurement and structural models was verified through SPSS 21.0 and AMOS 21.0, and the final model of the mediating effects was examined. Structural equation modelling was used to compare the fit of the research and alternative models, and found that the research model as the best model for the present data. Results of the study showed that needs for approval had indirectly a effect on interpersonal relationship via nunchi and social self- efficacy which also functions as a mediator. Social self-efficacy mediated effect of nunchi on interpersonal relationship. lastly, implications on consultation and follow-up studies are suggested.

신이식후 갑상선 기능변화에 대한 장기간 추적관찰

류미숙(Mee Sook Ryu),홍성표(Seong Pyo Hong),이태원(Tae Won Lee),임천규(Chun Gyoo Ihm),김명재(Myung Jae Kim) 대한내과학회 1997 대한내과학회지 Vol.53 No.4

N/A Background: Thyroid status in uremia is still inconclusive due to the complexicity of the system. No single pathogenetic event may explain the thyroid function abnormalities in end stage renal disease (ESRD). Defects at all levels of the hypothalamic-pituitary-thyroid axis have been identified. Regarding the thyroid dysfunction in ESRD it is well recognized that the TSH response to TRH is blunted and serum concentrations of thyroid hormones are decreased in patients with ESRD. Whether or not on maintenance hemodialysis. Restoration of renal function with renal transplantation resulted in normalization of all parameters of thyroid function with exception of blunted TSH response to TRH. We evaluated the long-term changes of the thyroid function in 10 patients to know whether the thyroid function and the hypothalamo-pituitary axis were improved with the recovery of the renal function under maintenance low-dosage steroid administration after renal transplantation. Methods: These tests were performed during the morning in the fasting state in 10 ESRD patients before, 1 month and 6 years after renal transplantation (RT). Thyroid function tests. Serum T3, T4 were measured by RIA kit and serum TSH was measured by IRMA kit. TRH stimulation test. Serum blood samples were obtained 0, 30, 60, 90, 120 min after TRH (400㎍) administration. Statistical analysis. All grouped data were expressed as mean±SD. Student t-test was used to assess the statistical difference between any two means. Results: 1) The mean basal level of serum T3 was reduced in ESRD patients (53.6±33.2ng/dL) and increased to the low normal level 1 month after RT (87.8±25.4ng/dL), improved to the normal level 6 years after RT (116.3±28.8ng/dL). 2) The mean basal level of T4 was within normal range before RT (5.9±1.1㎍/dL), after 1 month (6.2±1.2㎍/dL) and after 6 years (6.5±1.4㎍/dL) of RT. 3) The mean basal level of TSH was within normal range before RT (2.0±1.2μU/mL), after 1 month (1.1±0.7μU/mL), and after 6 years (0.7±0.5μU/mL) of RT. Rut the mean TSH level of 6 years of RT was significantly decreased within the normal range. 4) In ESRD the TSH response to TRH was blunted, had a diminished peak and delayed fall before RT. After 1 month of RT, the TSH response to TRH was persistently blunted, however showed more rapid fall of TSH. After 6 years of RT, the TSH response to TRH normalized, but the absolute level of TSH and the peak level of TSH to TRH were less than before and after 1 month of RT. Conclusions: The abnormalities of thyroid hormones in uremic patients were improved partially after 1 month of RT and almost completely after 6 years of RT. But the level of T3H and the peak level of TSH to TRH were low within normal range, these results may be a direct consequence of low-dosage and long-term glucorcorticoid administration.

폐농양 및 복부농양을 일으킨 Eikenella corrodens 감염 2예 : Two Case Reports and Review

정용희,류미숙,김영식,이우인,이희주,서환조 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

Eikenella corrodens is a slowly growing, facultative anaerobic gram-negative bacillus. It is a normal inhabitant of the human upper respiratory tract, and it is recognized as an infrequent cause of invasive disease. To date, a few cases of E. corrodens infection were noted from all other organ. We report herein two cases of E. corrodens infection. In one case E. corrodens was isolated in the lung abscess and in the other case it was in the appendiceal abscess. The patients were treated empirically with penicillin G. The results of the bacterial culture and sensitivity test were available with it. The patients were discharged 15 days later with marked improvement after treatment. Our report clearly demonstrates that E. corrodens can be the pathogen of lung abscess and intraabdominal abscess. We reviewed descriptions from the literature of infections caused by E. corrodens.