조직확장법,술중 조직확장법 및 술전 봉합법의 임상적 응용에 관한 비교 연구

오석준,하지운,김응춘,서인석,조세흠 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.3

The purpose of soft tissue defect reconstruction is minimal scar with aesthetic and functional good results. Reconstruction of head and neck, upper extremity, chest, and lower extremity defects has been reported with standard available tissue expansions. Various surgical methods have been used in an attempt to achieve a normallooking skin color, texture, sensation, thickness and same skin adenexa. Therefore, in accordance with defect size and location, the operative method was considered so we select adequate method. Authors achieved good results from 48 cases of tissue expansion, 18 cases of ISLE (intraoperative sustained limited expansion), and 35 cases of presuturing technique. 1)The most common cause of reconstruction for soft tissue defect was post grafted scar 51cases(51%), next was tattoo 20 cases(19.6%), traumatic soft tissue defect 16cases(15.7% ) and nevus 8 cases(7.8%)in order. 2)Males(55.9%)were affected more often than females(44.1%)in the ratio 1, 3 : 1 and the most frequently affected group was the age of 20 to 30 years as 36% . 3)The distribution of incidence and location were as follow : face(39.2%), upper extremity(25.5%), scalp(17.6%), lower extremity(9.8%) and trunk(7.8%). 4)The cause and distribution were scalp in 20 cases(39.2%), face in 40 cases(29.4%) in the post grafted scar, upper extremity 18 cases(90.0%) in the tattoo, upper extremity 4 cases(50.0%), face 2 cases(25.0%) in the nevus and even distribution in the traumatic soft tissue defects. 5)The presuturing technique was best recommended method for soft tissue defect in 4cm diameter lesion on extremity, but ISLE method was used in the open traumatic soft tissue defect. Over 9cm wide scar was resurfaced by expanded skin using tissue expander.

대형정신병원 입원환자의 수면제 처방에 관련된 인자

김현숙,서혜수,송인제,손진욱 대한생물치료정신의학회 1997 생물치료정신의학 Vol.3 No.1

만성화된 환자들이 주로 입원하여 있는 대형정신병원에서 수면제의 처방에 영향을 미치는 요소들을 조사해 보고자 하였다. 1996년 10월 2일 국립부곡정신병원에 입원해 있는 환자 402명을 대상으로 병록지조사를 통해 입원시부터 조사당일까지의 수면제 사용을 조사였다. 담당의사의 routine order에 의한 수면제 처방의 여부, 담당의사의 p.r.n. order에 의한 처방 여부, 당직의사에 의한 수면제 처방 여부를 조사하여 환자의 나이, 성별, 진단, 입원횟수, 유병기간, 담당의사의 정신과 근무 연수 등의 변수에 따라 비교해 다음과 같은 결과를 얻었다. 1) 207명(51.5%)이 재원기간동안 최소한 일회 이상 수면제를 복용하였고, 195명(48.5%)은 수면제를 복용한 적이 없었다. 2) 담당의사의 routine order에 의한 수면제 처방의 빈도는 남자에 비해 여자에서 높았다. 3) 담당의사의 routine order에 의한 수면제 처방의 빈도는 전문의가 담당의사인 환자군에서 높았다. 4) 담당의사의 p.r.n. order에 의한 수면제 처방의 빈도는 전공의 1년차가 담당하는 환자나 전문의가 담당하는 환자에서 높았다. 5) 연령, 진단, 유병기간, 입원횟수 등은 수면제 처방의 빈도와 유의한 관계가 없었다. 이상의 결과를 통해 국립부곡정신병원의 입원환자에서 수면제의 처방이 환자의 성별과 담당의사의 정신과 근무 연수와 관계됨을 알 수 있었다. The purpose of this study was to investigate the factors that were associated with the prescription of hypnotics in inpatients of a large mental hospital. The medical records were reviewed in 402 inpatients who were admitting in Bugok National Mental Hospital at October 2, 1996. The results were as follows: 1) Fifty-one percent of the whole group received a hypnotics at least once during hospitalization. 2) The prescription of hypnotics by routine order of doctor in charge was more frequent in female patients than male patients. 3) The prescription of hypnotics by routine order of doctor in change was more frequent in patients whose doctor in charge was a psychiatric specialist than patients whose doctor in charge was a psychiatric resident. 4) The prescription of hypnotics by p.r.n. order of doctor in charge was more frequent in patients whose doctor in charge was a first-year psychiatric resident or a psychiatric specialist than a second, a third, or a forth-year psychiatric resident. 5) Age, duration of illness, frequency of admission, and diagnosis were not significantly associated with frequency of hypnotics prescription. These findings suggest that the prescription of hypnotics in inpatients of Bugok National Mental Hospital was positively associated with patient's sex and duration of service of doctor in charge.

嶺湖南地方의 蒸發量分布에 關한 調査硏究

金仁湖,崔文錫,徐承德 慶尙大學校 1972 論文集 Vol.11 No.-

This report is statistically carried out the research on the distribution of evaporation from 1931 to 1970 in Yeungnam and Honam provinces in Korea. For this work, several data of evaporation were derived from Climatic table(for 1931∼'60) and monthly reports(for 1961∼'70) by thirty eight meteorological stations or observations which were instituted in Yeungnam and honam. Previously, the results and those distribution charts of evaporation from 1961 to 1970 are shown Table-2 and Fig. 1∼l4. Since mostly meteorological stations or observations in Yeungnam and Honam were hardly to retain all data of evaporation from 1931 to 1970, ten places(*marked places in Table-2) where they didn't comparatively occur to the lacks of measured evaporation for forty years(l931∼'70) were selected in the representatives in Yeungnam and Honam. Table 3 shows the distribution of evaporation in the representatives for forty years. Some differences are happend to numerical values of mean evaporation per year in the results between Table-2 and Table-3. However, if all of data are treated of reasonably statistical process, Table-2 and those charts(Fig. 1∼14) are presented obtaining considerations as a distributive tendency of evaporation there. From the conclusion of Table-2, mean value of evaporation per year in Yeungnam and Honam is proposed about 1,200mm

Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid

( Kicheol Yoo ),( Kang Yeol Suh ),( Gi Hun Choi ),( In-suk Kwak ),( Dong Kook Seo ),( Dohern Kym ),( Hyeon Yoon ),( Yong Se Cho ),( Hye One Kim ) 대한피부과학회 2017 Annals of Dermatology Vol.29 No.2

Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients. Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters. Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the sec-ond, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to de-termine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period. Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concen-tration of HSP70 on the first day and the length of the treat-ment period. No measurable concentration of IL-8 was de-tected before 5 hours, but the concentration started to in-crease after 11 hours. The peak value was measured on the fourth day. Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of in-flammatory mediators in burn blister fluid. (Ann Dermatol 29(2) 194∼199, 2017)

Cycloheximide가 膵臟腺房細胞에 미치는 影響에 關한 微細形態學的 硏究

徐仁壽,洪錫宰 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

蛋白合成障碍가 膵臟腺房細胞의 微細構造 및 自家貪食力에 미치는 影響을 알아보기 爲해 흰쥐에 cycloheximide와 自家貪食亢進劑인 neutral red를 單獨 또 倂合 投與하여 惹起된 微細形態學的 變化를 電子顯微鏡으로 觀察하였던 바를 要約하면 다음과 같다. Cycloheximide는 膵臟腺房細胞에 比較的 初期에서부터 Golgi complex의 腫脹, ER膜에 附着된 ribosome의 脫落을 主로 한 輕한 變化를 일으켰다. 60分이 經過하면 ER 및 mitochondria의 輕한 腫脹, 脫落된 ribosome의 破壞 및 消失等을 볼 수 있었으나 그 程度는 輕微하였다. Autophagic vacuole의 計數는 對照群이 한 腺房當 10.1±2.2個, neutral red投與群이 54.4±4.9個, cycloheximide와 neutral red를 同時에 投與한 群에서는 21.5±4.1개, cycloheximide投與 30分 後 neutral red 投與群에서는 9.7±2.7個이었다. 이와 같은 事實은 cycloheximide가 autophagic vacuole形成에 抑制的 效果가 있다는 것을 意味하며 autophagic vacuole의 形成에는 어떤 形態로든 새로운 蛋白合成이 必要하다는 것을 示唆하는 것이라 생각된다. This study was carried out to investigate the effects of cycloheximide, known as an inhibitor of protein synthesis at the level of polypetide elongation, on the fine structures of pancreatic acinar cells. Also studied was the effects of protein synthesis inhibition by cycloheximide on the neutral red-induced autophagocytosis. Twenty-one male albino rats, maintained on water only for 12 hours, weighing 200㎎. in average, were divided into 5 groups, 3 rats for each groups except Group 2. Group 1: This is to serve as a control group. Two consecutive doses of 0.4㎖. of saline per 100 gm. of body weight were given intraperitoneally to 3 rats at interval of 30 minutes. Group 2: A dose of 2 mg. of cycloheximide per 100gm. of body weight was given to 9 rats intraperitoneally and 3 animals each were sacrificed 30, 60 and 90 minutes after injection, respectively. Group 3: A dose of 2mg. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitoneal injection of 0.4 ㎖ of saline per gm. of body weight. Group 4: A dose of 2㎎. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitioneal injection of 2㎎. of cycloheximide per 100gm. of body weight. Group 5: Simultaneous intraperitoneal injection of 2 ㎎. of neutral red and 2㎎. of cycloheximide per 100gm. of body weight was given to 3 rats. All animals were sacrificed 60 minutes after the the last injection except Group 2. Pancreas extirpated for both light and electron microscopic studies. The results were summarized as follow: Cycloheximide induced moderate dilatation of Golgi complex aad detachment of ribosomes from RER membrane from early stage (sacrificed 30 minute after injection). Mild swelling of RER and mitochondria along with destruction and disappearance of detached ribosomes were noted in the animals sacrificed 60 and 90 minutes after injection (Group 2). The number of autophagic vacules per acinus was 10.1±2.2 in the control group (Group 1); 54.4±4.9 in neutral red only group (Group 3); 21.5±4.1 in neutral red simultaneous with cycloheximide group(Group 5); 9.7±2.7 in the group with neutural red administered 30 minutes after cycloheximide injection(Group 4). The results showed marked inhibitory effects of cycloheximide on the neutral red-induced autophagocytosis, suggesting need for do novo synthesis of protein in the formation of the autophagic vacuoles.

중금속 킬레이트 약의 만성 Cadmium 중독 흰쥐의 간장과 신장에서의 Cadmium 잔류량에 미치는 영향

이석호,서대규,신인철 漢陽大學校 環境科學硏究所 1993 環境科學論文集 Vol.14 No.-

중금속은 다른 물질과는 달리 체내에서는 생성되지도 파괴되지도 않으며 환경오염에 의해 공기, 물, 모양으로 침투되고 음식물에 혼입되기도 한다. 대부분의 중금속은 지질학적, 생물학적인 순환을 통해 자연적으로 재분포되며, 특히 납, 수은 및 cadmium(Cd)등은 과거에는 자연적인 순환이 생물학적인 순환을 능가해 왔지만 근대산업화 사회가 도래하면서 인간활동에 의한 생물학적인 순환이 급속도로 증가하면서 환경오염이 시작되었다. 본 실험은 Cd 투여로 유발되는 실험적 Cd 중독 Sprague-Dawley계 웅성흰쥐에서의 중금속 킬레이트 약 투여가 간장과 신장에서의 Cd 잔류량에 미치는 영향을 관찰하고자 시도하였다. 실험동물은 동일환경하에서 사육된 체중 200∼250mg의 Sprague-Dawley계 웅성흰쥐를 사용하였으며, 실험적 Cd 중독은 CdCl₂ (Signa사)의 1mg/ml의 생리식염수 용액을 사용하여 체중 kg당 1.5mg의 용량을 48시간 간격으로 3회 복강내 주사하여 유발시켰으며 1주일 후부터 72시간 간격으로 10회에 걸쳐 킬레이트 약을 복강내로 주사하였다. 적용한 킬레이트 약과 그 용량은 1,2-diaminocyclohexanetetraacetate(CDTA), disodium calcium ethylenediaminetetraacetate(EDTA), diethylenetriaminepentaacetate(DTPA), , triethylenetetraaminehexaacetate(TTHA), D-penicillamine(DPA), N-acetylpenicillamine(NAPA), 2,3-dimercaptopropanol(BAL), sodium 2,3-dimercaptopropanesulfonate(DMPS), sodium 2,3-dimercaptosuccinate(DMSA) 및 sodium diethyldithiocarbamate(DDTC) 등은 각각 0.1mmol/kg(BAL은 0.1mmol/kg)이다. CDTA, EDTA, DTPA 및 TTHA 등은 CaCl₂ 와 1 : 1 mole 비율이고 실험군은 적용한 킬레이트 약 별로 흰쥐 5마리씩을 배정하였고, 동량의 생리식염수를 주사한 군을 대조군으로 하였다. 킬레이트 약 주사 종료 3일 동물을 단두로 도살하여 개복한 후 간장과 신장을 적출하여 이들을 각각 0.1mg 씩 취하여 1.0ml의 질산과 1.0ml의 과염소산으로 전처리하여 조직추출액을 얻었고 이를 사용하여 polarized zeeman 원자분광흡광기(Atomic absorption spectrophotometer : Model Z-8100, Hitachi, Japan)로 조직내 중금속 잔류량을 flame 법으로 측정하였다. 간장에서의 Cd 잔류량을 DPA, EDTA, NAPA, DCTA, DDTC 및 DMSA 투여군에서 대조군보다 각각 32%, 23%, 19%, 17%, 16% 및 15%씩 감소하였고, 신장에서의 Cd잔류량은 DPA, DDTC, CDTA 및 EDTA 투여군에서 대조군보다 각각 33%, 21%, 18% 및 17% 씩 감소하였으며, 간장과 신장에서의 Cd 잔류량의 합계는 DPA, EDTA, DDTC 및 CDTA투여군 에서 대조군보다 각각 32%, 20%, 18% 및 17%씩 감소하였다. 이상의 결과로 여러가지 킬레이트 약 중 DPA, EDTA, CDTA 및 DDTC 등은 Cd 중독의 치료에 이용될 가능성이 많으나, DTPA, TTHA, BAL 및 DMPS 등은 Cd 중독에 치료에 큰 효과가 없는 것으로 사료된다. 그러나 기존의 실험들과 다른 결과가 나온 DTPA, TTHA, BAL 및 DMPS 등에 대해서는 더욱 깊은 연구가 필요하다고 사료된다. Sprague-Dawley albino male rats weighing 200 to 250gm were used. All animals were loaded with 3 intraperitoneal injections of cadmium chloride(1.5mg/kg) given at 48 hourly interval. Intraperitoneal injection of chelators commenced 1 week after the last loading injection and continued every 72 hour for a total of 10 injections. Chelators were given at a level of lmmol/kg(except, 0.01mmol/kg of BAL). The chelators used in present experiment are 1,2-diaminocyclohexanetetraacetate(DCTA), disodium calcium ethylenediaminetetraacetate(EDTA), diethylenetriaminepentaacetate(DTPA), triethylenetetramine hexaacetate(TTHA), D-penicillamine(DPA), N-acetylpenicilllamine(NAPA), 2,3-dimercaptopropanol(BAL), sodium 2,3-dimercaptopropanesulfonate(DMPS), sodium 2,3-dimercaptosuccinate(DMSA) , sodium diethyldithiocarbamate(DDTC). In order to establish the relative abilities of chelators to mobilized liver and kidney cadmium contents, at the end of the experiment the rats were sacrified and the livers and kidneys were immediately excised. A sample of approximately 0.1mg of tissue was digested in 1.0ml of nitric acid, followed by 1.0ml of perchloric acid, and the cadmium content of the samples was determined by atomic absorption spectrophotometer. The result obtained can be summarized as follow : 1. The residual liver cadmium content was reduced in rats administered DPA. EDTA, NAPA, CDTA, DDTC and DMSA(32%, 23%, 19%, 17%, 16% and 15% respectively)compared to control group. 2. The residual kidney cadmium content was reduced in rats administered DPA, DDTC, CDTA and EDTA(33%. 21%, 18% and 17% respectively) compared to control group. 3. The summation of residual cadmium content in liver and kidney was reduced in rats administered DPA, EDTA, DDTC and CDTA(33%, 20%, 18% and 17% respectively) compared to control group. These results suggested that DPA, EDTA, CDTA and DDTC might have protective role against the toxic effects of cadmium.

관동맥질환에서 각종 지질 및 지단백의 변화

황석순,김권배,손수인,서영숙,김기식,김윤년 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.3

To evaluate the relationship between coronary artery disease(CAD) and the various levels of lipid and lipoproteins, we performed coronary angiography to the patient, who were suspected coronary artery disease from January 1988 to August 1989. And compared it with normal control group. The number of control group were 41(21 men, 20 women), and the patient group were 52(42 men, 10 women). The age was not differ between two groups. The results were as follows; 1. In CAD group, the plasma concentration of total lipid, triglyceride, total cholesterol, phosphlipid, and TC/HDL ratio were significantly higher than in control group. But, though, the concentration of HDL was lower in CAD group, had no significant difference between two groups. 2. The severity of CAD were well correlated with total lipid, cholesterol, LDL, trigylceride, phospholipid, TC/HDL, LDL/HDL levels. HDL level didnt's show significant correlation with the degree of CAD. We concluded that total lipid, cholesterol, trigylceride TC/LDL ratio were valuable markers of CAD in Korean, but futhur studies of lipoproteiins and other risk factors in the large population will be recommended.

카드뮴이 인간 단핵구 세포주(THP-1)에서 cytokine 유전자 발현에 미치는 영향

신동훈,박관규,강미정,서석권,정인성,양선희 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.2

Cadmium, a potent toxic metal, posses a serious environmental threat but the mechanism of its toxicity remains unclear. Also, cadmium is a known immunotoxic agent in animal studies and induces pathophysiological effects by modulating components of immune system. Cytokines are being increasingly recognized as essential mediators of normal and pathologic immune response. Cells of mononuclear phagocytic system are strategically located at portals of entry in humans and therefore may be particularly at risk for cadmium exposure through contaminated air, food, and drinking water. In the present study, we investigated the effect of cadmium cytotoxicity for the monocyte and expression of cytokine gene in the control and cadmium treated human monocytic cell lines using RT-PCR method. The results showed that cadmium inhibited cell proliferation at 0.1mM cadimium treated cells for 24 hours. The TNF-α mRNA was expressed in both control and cadmium treated cells but not IL-6 and IL-1β. The mRNA levels of TNF-αwere examined during 24 hours culture period, at different time points. The expression of TNF-αmRNA increased in both 0.01mM and 0.1mM cadmium treated cells, but did not show dose-response relationship. According to cadimium treated duration, expression of TNF-αmRNA was more decreased in 24 hours than 6 hours. The decreased levels of mRNA of TNF-α suggest that cadmium suppresses its production at the transcription level.

자기 효능의 개념 분석

서순림,이은남,박송자,양영희,이동숙,최은옥,구미옥,김인자,이인숙,김성재,박영임,이은옥 서울대학교 간호대학 간호과학연구소 1997 간호학 논문집 Vol.11 No.1

Today human needs for health promotion and maintenance are increasing. The nursing progression has a great responsibility for helping clients to practice good health behavior. self efficacy was found to be potent predictor in initiating and maintaining the health-related behavior. Therefore, the concept is important in nursig intervention for change of health behavior. The purpose of this paper was to analyze and clarify the meaning of the concept, self efficacy. This study used Walder & Avant's process of concept analysis. Critical attributes of self efficacy were : 1) positive perception of his won capability; 2) intrapersonal strength; 3) possibility of being learned; 4) individuality; 5) specificity; 6) generalizability. Antecedents of self efficacy consist of those facts that 1) new situation occurs; 2) individuals have needs to change the new situation; 3) there are some required actions in new situation. Consequences occurring as a result of self efficacy consist of those facts that 1) individual initiates the of failure; 5) cope with the situation; 6) controls the situation; 7) the level of self efficacy is enhanced; 8) the level of self-esteem is enhanced.

복통으로 내원한 Behcet병 환자에서 진단된 연중독

남문석(Moon Suk Nam),정준표(Jun Pyo Chung),이홍우(Hong Woo Lee),오제열(Je Yol Oh),박효진(Hyo Jin Pack),이관식(Kwan Sik Lee),이상인(Sang In Lee),박인서(In Suh Park),이안나(An Na Lee),송경순(Kyung Soon Song) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.2

Behcets disease is a systemic disease involving skin, joint, gastrointestinal tract, vascular and nervous systems, characterized by recurrent oral and genital ulcerations and ocular in- flammation. The intestinal Behcets disease has nonspecific abdominal symptoms of colicky pain, constipation, diarrhea, nausea, vomiting, and malabsorption. Therefore, we need to dif- ferentiate with other diseases having nonspecific abdominal symptoms. We have experienced a case of lead intoxication after ingestion of herbal pills believed to contain lead compounds. A 40 year-old female with known Behcet's disaese was admitted due to acute colicky abdominal pain. The cause of abdominal pain and anemia was investigated and intestinal Behcets disease was ruled out. The diagnosis of lead intoxication was made by careful history, clinical manifestations, and various laboratory findings including peripheral blood smear, bone marrow aspiration and biopsy, serum lead and free erythrocyte protoporphyrin levels. W e also used lead mobilization test for measure of chelatable lead level. The treatment was performed with 4 courses of EDTA infusion therapy and the clinical symptoms and chemical data associated with lead intoxication improved.(Korean J Gastroen- terol 1994; 26: 399 404)