전통주 주박의 항혈전 활성 평가

김미선(Mi-Sun Kim),이예슬(Ye-Seul Lee),김종식(Jong Sik Kim),신우창(Woo-Chang Shin),손호용(Ho-Yong Sohn) 한국생명과학회 2014 생명과학회지 Vol.24 No.8

전통주 주박을 이용한 고부가가치 식품소재 개발을 위해, 상업적 시설에서 생산된 3종 약주(J-B, J-S, J-Y) 및 2종 탁주(J-H, J-W) 주박의 ethanol 추출물 및 열수 추출물을 조제하고 이들의 혈액응고 저해활성, 혈소판 응집저해 활성 및 인간 적혈구 용혈활성을 평가하였다. 5종 주박의 pH는 3.90~4.29로 유사하였으나, brix는 5.0~27.0으로 다양하게 나타났으며, 수분 및 알코올 함량에서도 시료에 따라 1.8배의 차이를 나타내었다. 주박의 색차와 성분은 첨가된 부재료 및 사용누룩에 좌우되었으며, J-W 주박의 경우 수분함량이 80.3%, brix 13, 알코올 함량 1.8%를 함유하여 다른 주박에 비해 다양한 식품제조에 용이하게 이용 가능하리라 판단되었다. Ethanol 추출효율은 J-H, J-W, J-B, J-S, J-Y의 순, 열수 추출효율은 J-S, J-B, J-W, J-H, J-Y의 순으로 높았으며, 총폴리페놀 및 총플라보노이드함량은 ethanol 추출물 중에서는 J-H, 열수 추출물 중에서는 J-Y 주박에서 가장 높았다. 5종 주박의 10종 추출물은 모두 5 mg/ml 농도까지 인간 적혈구에 대한 용혈활성이 나타나지 않았으며, J-B, J-S, J-Y의 약주 주박의 ethanol 추출물에서 유의적인 혈액응고저해 활성이 나타났으며, J-W 탁주 주박의 열수 추출물에서 thrombin 저해 활성과 J-B, J-S 및 J-H 주박 열수 추출물에서 혈액 응고인자 저해활성을 확인하였다. 혈소판 응집저해 활성평가의 경우 J-W 탁주 주박의 ethanol 및 열수 추출물에서만 아스피린에 필적하는 우수한 활성이 확인되었다. 본 연구결과는 다양한 약주 및 탁주 주박이 항혈전 활성을 가지고 있으며, 주박으로부터 항혈전제 개발이 가능함을 제시하고 있다. In this study, ethanol and hot water extracts of lees from Korean traditional wine (J-B, J-S, J-Y, J-H, and J-W) were prepared, and their effects on blood coagulation, platelet aggregation, and hemolysis of human red blood cells (hRBCs) were investigated to develop functional food ingredients from lees. The pH and brix of the lees ranged from 3.90 to 4.29 and 5.0 to 27.0o, respectively, and there was a huge difference in the water and ethanol content among the lees. The nuruk and additives used affected the color and physicochemical properties of lees. The J-W takju made from only rice and traditional nuruk, which has 13o brix and 1.8% of alcohol, has potential as functional food ingredient. With regard to the extraction yields of lees, higher yields were obtained from J-H, which contains different medicinal plants, in ethanol, followed by J-W, J-B, J-S, and J-Y. Higher extraction yields of lees were obtained from J-S in hot water, followed by J-B, J-W, J-H, and J-Y, respectively. The ethanol extract of J-H and the hot water extract of J-Y had the highest contents of total polyphenol and total flavonoids among the lees extracts. The 10 lees extracts did not show hemolysis activity against hRBCs up to 5 mg/ml. In an anticoagulation activity assay, the ethanol extracts of three yakju lees (J-B, J-S, and J-Y) and the hot water extract of J-W inhibited thrombin activity, whereas the hot water extract of J-B, J-S, and J-H inhibited blood coagulation factors. In an antiplatelet aggregation activity assay, only the J-W takju lees showed significant inhibition activity. Our results suggest that lees from traditional wine had high potential as a novel antithrombosis agent.

농촌 자활사업참여자의 음주 문제 개입프로그램 개발: 동기강화상담의 적용

이종례(Jong Rea Lee),장수미(Soo Mi Jang),허성희(Seong Hui Heo) 한국지역사회복지학회 2022 한국지역사회복지학 Vol.- No.83

본 연구의 목적은 동기강화상담(Motivational Interviewing: MI) 모델을 적용하여 농촌 자활사업참여자의 음주 문제 개입프로그램을 개발하는 것이다. 개입연구(Intervention Research: IR)의 절차에 따라 문헌고찰, 실무자 인터뷰를 시행한 후 농촌 자활사업참여자의 음주문제 양상을 파악하고, 동기강화상담의 원리와 전략에 기반하여 1차 프로그램을 구성하였다. 전문가 자문을 통해 수정 보완 후 2차 프로그램을 도출하였고, 음주 문제를 가진 농촌 자활사업참여자를 실험집단(n=10)과 통제집단(n=10)으로 무작위 배치한 후, 실험집단에 동기강화상담 기반 음주 문제 개입프로그램을 실시하였다. 사전‧사후‧추후 조사를 통해 개입프로그램의 효과성을 평가한 결과, 실험집단의 음주 지식, 단주 자기효능감, 변화 동기, 음주 문제, 자활 성과의 사전‧사후 점수에서 유의미한 변화가 발생하였고, 음주 문제를 제외한 모든 변인에서 사전‧추후 점수의 유의미한 변화가 나타났다. 음주 문제의 경우 사전‧추후, 사후‧추후 모두 유의미한 변화가 발생하지 않아 효과성이 유지되지 않음을 확인하였다. 이후 현장 실험 결과에 대한 피드백을 반영하여 최종(3차) 프로그램을 개발하였다. 결론에서는 주요 연구 결과를 바탕으로 논의하고, 자활 현장을 중심으로 사회복지 실천 및 정책에의 함의를 제시하였다. The purpose of this study is to develop an intervention program for the drinking problem of participants in rural self-sufficiency center by applying the Motivational Interviewing(MI) model. According to the stages of Intervention Research(IR), the aspects of drinking problems of rural self-sufficiency participants was identified through literature review and interviews with practitioners, and the 1st program was constructed based on the principles and strategies of MI. After revising with experts advice, a 2nd program was derived, and rural self-sufficiency participants with drinking problems into an experimental group(n=10) and a control group(n=10) by random assignment, MI was conducted only in the experimental group. As a result of the program, significant changes occurred in the pre and post scores of the experimental group’s drinking knowledge, abstinence self-efficacy, change motive, drinking problem, and self-sufficiency outcomes, and significant changes in pre and 6 weeks later scores were found in all variables except for drinking problems. The drinking problem was not significant in pre and 6 weeks later, post and 6 weeks later, it could be seen that the effectiveness was not maintained. The 3rd(final) program was developed by evaluating the effectiveness of the programs, and reflecting feedbacks from field experiment. In conclusion, the implications for social welfare practices and policies were presented, focusing on the field of self-sufficiency.

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

대구지역 영세사업장의 작업환경 측정실태와 인식도

이성미,이종영,김두희,이종화,장봉기 순천향대학교 산업의학연구소 1996 순천향산업의학 Vol.2 No.1

This study was conducted to examine both actual status and understanding of the workshop environment of the small sized companies with 30 or less employees in and around Taegu city, Korea by analysing the questionnaire with 400 small sized manufacturing companies from the end of Mar. to the end of June 1993 according to their employee number and business type. The result of this study is summarized as follows. The job title of the respondents showed that the president accounted for 34.3% which was highest, particularly, which showed the highest percent in the companies with 5-9 employees. The management of workshop environment indicated that the response of 'Very Necessary' accounted for 52.0% which was higher. Thus it was shown that the owner and manager's understanding of the workshop environment was higher. However, the response of 'No Enough Money' for investment in improvement of workshop environment indicated that the president accounted for 51.1% which was also higher. 54.3% of all subjects considered their workshop environment as not good, while 66.5% answered that the workshop environment has effect on the health of employees. The actual status of industrial accidents occurred from January to December 1992 showed 18.7% frequency, while their business type was that the metal and machinery industries accounted for 24.8%. In addition, their size indicated that 16-29 employees accounted for 26.5% which was higher than that of other companies with different sizes. Regarding the question of understanding whether their workshop is the subject required for measuring the workshop environment, it was shown that the response of 'No Understanding' accounted for 57.0%. Based on their size, the companies with 5-9 employees accounted for 37.6%, while on their business types the metal and machinery industries accounted for 31.2% which showed the lowest understanding. In case of the companies understanding as the subject required for measurement of their workshop environment, the harmful element acting as a problem in their workshop indicated that the response of 'Too Noisy' accounted for 37.8%, and 'Too Serious Dust' for 11.8%, when the noise appeared as the most serious harmful element in all workshops. Among the companies required for measuring the workshop environment, it was shown that the workshops which did not carry out the required measurement accounted for 24.4%. Of the workshops which carried out the measurement required for their workshop environment, the frequency of annual two times accounted for 40.1% which was highest. Based on their size, it was shown that the companies with 16-29 employees accounted for 52.6% which was highest. The use of their measured data and execution of required improvement showed that the response of 'Good' was totally high. Also the keeping result of their measured list indicated that the response of 'Good' accounted for 72.3%. Based on the above result, the understanding of needs for control of workshop environment was high. However, the actual improvement of workshop environment was not so active as their understanding. Because the health of all employees is closely related to the harmful workshop environment and working conditions, it will be necessary for the government to support the improvement of workshop environment of these small sized companies with insufficient capability of investment, to suggest its direction and to continuously encourage and inspect their measurement of workshop environment.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

Southern Blot Hybridization법을 이용한 악성 림프종의 면역유전형에 관한 연구

전호종,기근홍,김윤신,이미숙,이미자,장원재,장숙진,박영진,정춘해,정종훈,양성훈,이광민 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

Immunogenotyping using gene rearrangement analysis has emerged as a precise laboratory aid in the diagnosis and classification of malignant Iymphoid neoplasms. The lineage and clonality of the malignant Iymphoid neoplasms can be identified by the demonstration of rearrangements of antigen receptor genes of the immunoglobulin and T-cell receptor genes. The analysis of the gene rearrangements on the malignant Iymphoid neoplasms are also useful as a sensitive unique clonal markers to detect early recurrence in patients with malignant Iymphoid neoplasms after treatment. To analyze the sensitivity and specificity of gene rearrangements in the diagnosis of malignant Iymphoid neoplasms. 24 cases of malignat Iymphoma were examined by Southern blot hybridization using CTβ-T cell receptor β chain gene-DNA probe and JH-immunoglobulin heavy chain gene-DNA probe. The results of the immunogenotypings using Southern blot hybridization disclosed high correlation between the immunophenotyping using immunohistochemical stain with monoclonal antibodies (B-cell Iymphoma 84.2%. T-cell Iymphoma 75% ). The analysis of the gene rearrangement of the angioimmunoblastic lymphadenopathy(AILD) and unclassifiable Iymphoma using immunohistochemical stain could resolve the monoclonality and lineage . Rearranged bands to the CTβDNA probe were observed in one case out of 2 cases of AILD. One case of unclassifiable Iymphoma showed rearranged bands to the CTβ DNA probe. There were no rearrangements in reactive follicular Iymphoid hyperplasia and paracortical Iymphoid hyperplasia. In conclusion, DNA gene rearrangement study should be applied to differentiate the clonality and cell lineage in the malignant Iymphoma with indistinctive immunophenotype.

박송암제 화청에 관한 음악 연구

이종미(Lee Jong-Mi) 한국전통음악학회 2007 한국전통음악학 Vol.- No.8

Hwachoeng is the musical result of missionary activities of Buddhism as a trial to make Buddhism popular. It is a precious cultural asset to Korean Music. It is considered to be worthwhile to study as music in the form of a song with ethnic emotion from the prehistory times to the modern times. Here are the summary of the results of the disscussion up to now. First, Hwacheong means the invitation to Bulbosal. It became a song by putting ‘Yul’ on the pre-existing verse. All the buddhist songs except Beompae can be classified as Hwacheong. As anyone can create it because ‘Yul’ goes freely, we can classify it as popular music. Second, when we see its musical characteristics of Hwacheong, tune and musical scale is Major Key D, which is similar to Folk Song Tune and Musical Scale of the Eastern Part such as Menarijo that uses the three keys Mi-Ra-Do. Among them Ra is most frequently used. Third, rhythm is mostly 9/8 and sometimes we can see other rhythms such as 4/8, 12/8, and 15/8. For the pattern, it does not have special pattern unlike other musical notes. It has the pattern of A-A'-A''-A'''. Fourth, Hwacheong is classified as free style music. From the analysis of the collected notes, we could confirm that they do not have uniform or consistent rhythm or phrase. Although they have the same words in the first phrase, they have different verse from the second phrase. Hwachong can be considered as our unique and original music in our history. We need to try diverse and various approaches to it, perceiving it as inclusive and pure music in our musical history.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

Discordance in Spirometric Interpretations Based on Korean and Non-Korean Reference Equations

Nami Kim,Mi-Hee Park,Se-yeong Kim,Chunhui Suh,Sangyoon Lee,Kun-Hyung Kim,Chae-Kwan Lee,Dae-Hwan Kim,Jong-Tae Lee 대한직업환경의학회 2013 대한직업환경의학회지 Vol.25 No.-

Objectives: Korean regression models for spirometric reference values are different from those of other ethnic groups. The purpose of this study was to evaluate discordance in spirometric interpretations based on using Korean reference equations versus non-Korean reference equations. Methods: Spirometry was performed on 825 Korean male workers from April 2009 to November 2011. The spirometric patterns and disease severity were evaluated using two Korean equations (Choi’s and Lee’s) and three equations for Caucasians (NHANES III, Morris’s, and Knudson’s), and the results of Choi’s equation were compared with the non-Korean equations. The spirometric patterns were defined as normal, restrictive, and mild and moderate obstructive. Results: The mean differences in the FEV1% and FVC% between the two Korean equations were 2.0 ± 1.3% and 3.5 ± 2.2%, respectively. Morris’s equation had the greatest difference in the FEV1% from Choi’s equation: 32.9 ± 8.5%. Knudson’s equation had the greatest difference in the FVC% from Choi’s equation: 10.5 ± 6.5%. Conclusions: The two Korean equations shared similar characteristics of spirometric interpretation. The spirometric interpretations of Choi’s equation were significantly different from those of Morris’s equation and Knudson’s equation.

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea