인삼의 GAP (우수농산물인증) 관련요소 분석

유용만,오세찬,성봉재,김현호,윤영남 한국약용작물학회 2007 한국약용작물학회지 Vol.15 No.3

우리나라 인삼의 대표적 재배지인 금산군에서 GAP인삼 생산을 희망하는 10농가를 선발하여, GAP인삼의 안전성에 영향을 미칠 수 있는 농약 및 중금속의 잔류 그리고 미생물 등의 위해요소가 오염되었는지 여부를 검토하였다. 검토 대상은 경작되고 있는 인삼을 비롯하여 인삼밭의 토양, 수질 등 3종류로 하였다. 토양에서의 농약잔류를 검사한 결과, 인삼농가 및 일반농가 토양에서 자주 사용되는 Carbendazim등 81개의 농약을 선발하여 분석하였으나, 인삼에 등록된 농약별 최대잔류허용수준을 초과하지 않고 모두 적합판정의 결과로 나타났다. 또한 인삼재배 토양에 중금속의 오염우려가 되는 비소 등8종류의 조사 하였으나 기준치 보다 적게나와 전부 적합 판정을 받았다. 한편 관개수 및 농약희석 살포 등으로 사용되는 수질을 조사한 결과도 조사된 10농가가 모두 안전하였다. 참여농가 밭에서 직접 채취한 인삼에서 24종의 농약을 분석한 결과도 적합 판정으로 나타났다. 조사농가 수삼에 존재하는 위해성 미생물 검사에서 Pantoea spp등 2종의 Gram 음성균과 1종의 그람양성균(Staphylococcus sciuri)이 동정되었으며, 일반세균 수는 1.5×103 cfu/ml 로 다른 농산물보다 적은 수로 발견되었다. 따라서 이번 조사한 금산 지역에서는 GAP 인산재배 생산에 적합한 토양, 수질을 보였으며, 기존 생산한 인삼에서도 GAP 농산물 생산 기준에 적합하였다. For the analysis of hazard factors and the development of GAP (Good Agricultural Practices) Ginseng, 10 ginseng cultural farms wished certified GAP were selected at Geumsan-gun area, a representative site of ginseng cultivation in Korea. In order to verify the safety of GAP ginseng, possible contamination of pesticide and heavy metal residues, and microbial hazard were analyzed. Soil and water around ginseng cultivation field, and ginseng were investigated. Eighty-one pesticides including carbendazim were used as typical pesticide against plant pathogens and insect pests of ginseng plant and general crops. There was no excess the maximum residue limit (MRL) in residue figure of the soil. Including the residue figure of the arsenic (0.81 ml/kg) and 7 other heavy metals was also suitable to cultivate the ginseng plant. The irrigation water and dilution water for pesticide application were also safety level for GAP. Fresh ginsengs from the farms were sampled and investigated pesticide residues and contaminations of bacteria. Among 23 pesticides tested, we didn't detect any kinds of pesticide residues, but tolclofos-methyl was frequently found in the other ginseng field. On the investigation of microorganism hazards, 2 gram negative bacteria and 1 gram positive bacterium were found in the fresh ginseng. Number of total bacteria was 1.5×103 cfu/ml, which was less than the other agriculture products. At these results, 10 selected ginseng farms were good cultural places for GAP ginseng production and the ginseng cultured from Geumsan-gun area were a good safe far human.

유통중인 파프리카, 딸기 및 토마토의 생물학적 위해요소 분포 조사

유용만,윤영남,Quan Juan Hua,차광호,이영하 한국식품위생안전성학회 2009 한국식품위생안전성학회지 Vol.24 No.2

The consumption of “ready-to-eat” agricultural products is recently increasing and the safety of these agricultural products is forefront of public concerns. The 120 samples of paprikas, strawberries and tomatoes, which are the representative exported agricultural products, were purchased at the department stores and discount stores in Daejeon. And we determined the microbiological and parasitological contamination level of these agricultural products using culture media, multiplex PCR, commercial bacterial detection kit and microscopy, and also evaluated the decontamination method. Mean counts of total aerobic bacteria from these agricultural products ranged from 1.3×104 CFU/g to 1.8×105 CFU/g, and mean counts of coliforms ranged from 1.4×103 CFU/g to 9.6×103 CFU/g. There was no significant difference in the level of bacterial contamination between the agricultural products from department stores and the ones from discount stores. Strawberry showed the highest contamination level for the bacteria and we also found the unidentified parasite eggs. Enterobacter cloacae was the most frequently isolated bacteria strain, but no food poisoning pathogenic bacteria except Staphylococcus aureus was isolated from the products by multiplex PCR. Compared to unwashed products, tab water-washed ones showed 80% decrease of the counts of total aerobic bacteria on the agricultural products, and the rates decreased more by incorporating detergent or ultrasonic wave treatment. We concluded that the biological contamination levels among paprikas, strawberries and tomatoes were the highest in strawberries, but there were not significant difference according to distribution systems.

LED 광원에 따른 미세조류 Scenedesmus obliquus의 성장 특성

유용진,김송이,이건우,이영복,김진우,김호섭 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.11

Microalgae are independent organisms that perform photosynthesis and can alter the culture environment to increase accumulation of useful substances derived from microalgae. In this study, cell growth was measured by incubation for 39 days using MBBM, Neo medium, and seven light sources, which is the main factor affecting cell growth of microalgae S. obliquus. In the case of S. oliquus, which grew in MBBM and Neo medium, cell growth was highest under fluorescent light sources and Red2 LED (R660) light sources, and cell growth was lowest under Infra Red LED (R741) light sources. The average cell growth rate was 17.7% for MBBM and 15.4% for Neo. Comparing the effects of dry cell weight of Neo medium containing nutrients on the production of aquatic plants, MBBM and dry cell weight of Neo resulted in higher cell growth than Neo medium under all LED light sources except for Blue LED (B450). This proves that MBBM is more suitable for increasing the cell growth of microalgae than Neo medium and confirms that light source selection is important in the production of useful materials through mass cultivation of microalgae in the future. 미세조류는 광합성을 수행하는 독립영양생물로 배양 환경을 변화시켜 미세조류 유래 유용물질의 축적을 증대시킬 수 있다. 본 연구에서는 미세조류 S. obliquus의 세포 성장에 주요 인자인 배지와 광원의 영향을 평가하기 위해 MBBM, Neo 배지와 7가지의 광원을 사용하여 39일 동안 배양하여 세포 성장을 측정하였다. 그 결과 MBBM과 Neo 배지에서 성장한 S. obliquus의 경우, Fluorescent light 광원과 Red2 LED (R660) 광원에서 세포 성장이 가장 높게 관찰되었고, Infra Red LED (R741) 광원에서는 세포 성장이 가장 낮게 관찰되었다. 평균 세포 성장률은 MBBM 배지에서 17.7 %, Neo 배지에서 15.4 %의 성장률을 확인하였다. MBBM과 수생식물 생산용 영양소를 포함한 Neo 배지의 건조세포무게(dry cell weight)를 비교하였을 때, Blue LED (B450)를 제외한 LED 광원에서 Neo보다 많은 세포 성장이 높은 것이 확인되었다. 이는, MBBM 배지가 Neo 배지보다 미세조류 세포량 증대에 보다 적합함이 증명되어 향후 미세조류 대량배양을 통한 유용물질 생산에 있어 광원 선정이 중요하다는 것이 확인되었다.

생화학 농약의 개발 및 관리 동향

유용만 한국농약과학회 2003 한국농약과학회 학술발표대회 논문집 Vol.- No.-

Bacillus thuringiensis 가 생산하는 독소단백질

유용만 忠北大學校 遺傳工學硏究所 1989 遺傳工學硏究 Vol.3 No.1

국내외 언론에 비친 6 · 25 전쟁

庾龍源(유용원) 관훈클럽 2000 관훈저널 Vol.- No.75

유익성 극대화, 유해성 최소화 방안

유용만 한국농약과학회 2000 한국농약과학회 학술발표대회 논문집 Vol.- No.-

Suppression of Meloidogyne incognita in Lettuce and Oriental Melon by Pasteuria penterans KW1

유용만,조명래,주영재,박덕환,허장현,ChunKeunLim 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.3

Pasteuria penetrans KW1 (PP), parasitic bacterium of nematode, was isolated from oriental melon greenhouse soil in Korea and evaluated for the suppression effect on the reproduction of southern root-knot nematode, Meloidogyne incognita (MI), in lettuce (Lactuca sativa L. var. Chungchima) and oriental melon (Cucumis melo L. var. Eunchun). Pot experiments were conducted by planting the lettuce seedlings in medium inoculated with 5,000 MI juveniles/pot (J), J + 100,000 PP endospores/ 1 g medium, and J + 200,000 PP endospores/1 g medium. After 11 weeks of plantation, number of root galls in J + 200,000 PP endospores/1 g medium was decreased to 92/root (38.9%, control effect), compared to the J of 150/root. In the second plantation of lettuce in the same pots, the numbers of root gall were significantly decreased in PP treated pots with 75 (77.2%, control effect) and 150/root (54.4%, control effect) in J + 200,000 and J + 100,000 PP endospores/1 g medium, respectively, compared to the J of 330/root when harvested at 10 weeks after planting. In oriental melon, root gall percentages were 32.1 (60.2%, control effect) and 52.9% (34.5%, control effect) in J + 200,000 and J + 100,000 endospores/1 g medium which were significantly lower than that of 80.7% in J.

환영사

유용만 한국약용작물학회 2014 한국약용작물학술대회 발표집 Vol.2014 No.05

수출농산물의 안전성 향상을 위한 연구개발 방향

유용만 한국농약과학회 2009 한국농약과학회 학술발표대회 논문집 Vol.2009 No.2