RAPD법을 이용한 양파품종(Allium cepa L.)간의 유전변이와 유연관계분석

양보경,김도훈,이영병,남재성,정순재 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

This study was carried out to investigate genetic variation and relationships among onion varieties, Allium cepa L., by RAPD-PCR analysis. In the test of 120 primers with 58 onion varieties collected from local and abroad, eight primers turned out to be useful for further analysis. These primers eventually provided a total of 71 bands, consisting of 68 polymorphic and three monomorphic ones. The polymorphic bands were subjected to phylogenetic analysis using NTSYS and PAUP computer programs. In the NTSYS analysis, similarity coefficient of the 58 onion varieties ranged from 0 to 0.9179 and that of 27 varieties with obvious origin ranged from 0.0306 to 0.9032, The dendrogram of 58 varieties obtained by NTSYS analysis resulted in 15 groups, but overall groupings were not consistent with bulb phenotypes (e.g., color, weight, and shape) or origin of the varieties either In the PAUP analysis, genetic divergence of the 58 onion varieties ranged from 0.042 to 0.532 and that of 27 varieties with obvious origin ranged from 0.042 to 0.394. Phylogenetic analysis using PAUP revealed 13 group with 58 varieties and nine group with 27 varieties, but these were not consistent with any bulb phenotypes or origin either, as seen in the NTSYS analysis.

Streptozotocin 유도 당뇨쥐에서의 Phospholipase A₂, Cyclooxygenase 활성과 Thromboxane 및 Prostacyclin합성

양정아,김성옥,최정화,곽오계,이순재,장현욱 영남대학교 약품개발연구소 1998 영남대학교 약품개발연구소 연구업적집 Vol.8 No.-

당뇨병에서의 혈전생성 기전에 관련된 주된 요인을 관찰코져 흰쥐를 4주간 사육한 후 streptozotocin(STZ)으로 당뇨를 유발한 후 6일째에 희생하여 당뇨쥐에 있어서의 phospholipase A₂ 활성변화에 따른 조직의 과산화적 손상과 혈소판 응집능 등의 변화를 관찰한 결과는 다음과 같다. 체중증가는 STZ를 투여한 후에는 STZ 투여군에서 현저하게 감소하였다. 식이섭취에 있어 STZ injection 후, DM군은 16% 증가하였다. 식이효율은 STZ injection 후 DM군은 정상군에 비해 유의적으로 감소하였다. 혈청 중의 총 지질과산화물가는 정상군에 비해 DM군은 2.5배 높았다. HDL 중의 TBARS는 정상군에 비해 DM군은 약 1.6배 정도 높았고 LDL 중의 TBARS는 정상군에 비해 DM군에서 4.8배의 높은 증가를 보였다. 혈소판 phospholipase A₂ 활성은 DM군은 정상군에 비해 42% 증가하였다. 혈소판 cyclooxygenase 활성은 정상군에 비해 DM군이 2배 정도 높은 수준이었다. 혈소판 중의 TXA₂ 농도는 정상군과 비교하여 DM군에서 169%로 현저하게 높았고 대동백 PGI₂의 농도는 정상군에 비해 DM군은 낮은 수준을 보였으며 대동맥 PGI₂와 혈소판 TXA₂의 비는 DM군에서 정상군에 비해 55% 낮았다. 결론적으로 STZ 유발 당뇨쥐에 있어서는 혈청 지질과산화물 수준의 증가와 더불어 phospholipase A₂ 활성이 증가되었으며 따라서 cyclooxygenase가 유도하는 AA cascade 활성화에 의해 TXA₂ 생성증가 및 PGI₂/TXA₂ ratio의 감소가 관찰되었다. 이러한 결과는 당뇨병 질환에서 나타나는 동맥경화증, 심혈관계 질환 등의 여러 혈관계의 병리적 현상들이 당뇨 상태에서의 지질대사 이상으로 인한 지질과산화물의 증가와 그의 독성으로 인한 여러 혈소판 관련 인자들의 활성화와 밀접하게 연관되어 나타나는 것을 시사하는 것이라 하겠다. The relation between lipid peroxidation and thrombotic reaction were investigated in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 100±10gm were randomly assigned to normal and STZ-induced diabetic group(DM). Diabetes was experimentally induced by intravenous injection of 55mg/kg of body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of basal diet. Animals were sacrificed at the 6th day of diabetic states. Body weight gains were lower in diabetic group after STZ injection. Serum levels of thiobarbituric acid reacting substances(TBARS) that were markedly increased in DM group compared with of normal group. TBARS levles of HDL and LDL were similar patterns to total TBARS of serum. Activities of platelet phospholipase A₂(PLA₂) were higher in diabetic group than those of normal group. Activities of platelet cyclooxygenase were 106% in DM group than normal group. Platelet thromboxane A₂(TXA₂) formation was increased in DM group than normal group. Production of aortic prostacyclin(PGI₂) was lower in diabetic group than that of normal group. PGI₂/TXA₂ ratios were decreased by 55% in DM groups than those of normal group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stess which leads to acceleration of lipid peroxidation and platelet aggregability. In conclusion, accelerating effect of lipid peroxidation and thrombogenesis in diabetic state is regarded to be resulted from enhancement of PLA₂ activity and arachidonic acid metabolism, inhibition of antiaggregating agent and aortic PGI₂ formation.

후두백반증의 임상-병리학적 검색 -추적 생검 및 p53과 PCNA의 발현 양상

박양순,옥순애,공경엽,허주령,유은실,이인철,김상윤,최기영 대한병리학회 1996 Journal of Pathology and Translational Medicine Vol.30 No.4

Cementation 법에 의한 흑연 분말상의 구리 코팅

양승봉,민봉기,정동호,최순돈 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.2

We studied the copper cementation and homogeneous coating on the graphite powder by changing the activation temperature and the amount of wetting agent. When we peformed the activation process above 380℃, we were able to achieve the homogeneous copper coating layer on the graphite powder. However, it was impossible to get the homogeneous coating layer on the graphite powder without the activation process. In addition, wetting process on the graphite powder is essential for improving the efficiency of copper cementation. As a result, the optimum condition for copper coating on the graphite by cementation is to add 1㎖ glacial acetic acid and mix with 7g copper sulphate(CuSO4 · 5H2O) and 2g zinc.

마우스의 면역반응에 미치는 백모등엑스의 효과

梁在憲,殷在淳,韓相贊 又石大學校 1995 論文集 Vol.17 No.-

Solanum lyratum(SL)has been used as a herbal drugs for an anticancer, antiinflammatory, antitoxic agents, ect. The present study was to carried out to investigate the several immune-modulatory effects of Solanum lyratum extract on histamine biosynthesis, lymphocyte blastogenesis, and hemolytic plaque forming cell assay against to in C57BL/6 mice. Histamine content lymphocyte blastogenesis and hemolytic plaque forming cell were deter mined by enzyme-isotope method, (^3H)Thymidine incorporation and cunningham method, respectively. Cytotoxicity, which was showed as 50% mortality was occurred by the treatment of SL(10^-3g/ml, 48hr incubation). Histamine production was significantly increased by the addition of 1.0㎍/ml of Con A after 48hr incubatin. SL suppressed histamine production at all concentration of SL(10^-9∼10^-3g/ml). Con A(0.1㎍/ml)dependent T-lymphocyte proliferation was significantly increased by the addition of 10^-7g/ml of SL. And antibody formation markedly increased at 5×10^-4g/㎏. This results suggested that SL have an enhancement action of the immune response, SL in creased cell mediated immune response and also enhanced antibody production(IgG, IgM).

프로스타글란딘 E₁에칠에스테르의 외용 리오겔 제제 설계

양성운,이진교,이지은,김희규,박혜숙,김종석,최한곤,용철순,최영욱 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

External lyogels containing prostaglandin E₁ethyl ester(PGE₁-EE), a produrg of prostaglandin E₁(PGE₁) as a therapeutic agent for erectile dysfunction, were formulated to overcome the aqueous instability and enhance the percutaneous absorption. Lyogels of PGE₁-EE were prepared with ethanol (EtOH)/proplyene glycol (PG) cosolvent system as a vehicle, cineol as an enhancer, and hydroxypropylcellusose as a gelling agent. In vitro percutaneous absorption studies were performed to determine the rate of PGE₁ absorption through rat or hairless mouse skin. The permeability of PGE₁-EE lyogel with enhancer was 16-fold greater than that of lyogel without enhancer. Cosolvent produced 9-fold increase in percutaneous absorption. Pharmacodynamic effects of lyogels were evaluated in mature male cats in terms of intracavernosal pressure (ICP). Lyogles containing 0.01% of PGE₁-EE showed higher ICP compared to intraurethral preparation of PGE₁(1%) and enhancer-free control lyogel. The shelf-life (t_(10%) of lyogel at refrigerated condition (4℃) was calculated as 928 days, which is 4.2 times longer than that of control hydrogel. As a result, PGE₁-EE was formulated successfully to a lyogel system with a selective enhancer and cosolvent system for the topical delivery of PGE₁.

血淸蛋白質의 電氣泳動樣相에 의한 食餌蛋白質의 營養學的 硏究

梁且範,朴尙基,文南順 漢陽大學校 韓國生活科學硏究所 1989 韓國 生活 科學 硏究 Vol.- No.7

Nutritional quality of protein diet was evaluated by serum protein pattern of albino rats which were fed for 15 days with different protein sources and levels. The results are as follows 1.Albumin contents of serum protein might be used for evaluation of the quality of the protein sources and prediction of the least requirement of protein for various protein sources. 2.A / G ratio of serum protein of all experimental animals was not showed steady tendency for all diets treatments. 3.The area ratio of albumin fraction in electrophoretic pattern was useful for estimation of the albumin content of the serum. 4.The peak height ratio of Hpf / Hpb of serum protein fraction in electrophoretic pattern could be used for prediction of the protein deficiency and established the basis of the least requirement protein content for growth. 5.The peak area ratio of Tf / Fa of serum protein fraction in electrophoretic pattern may be useful as an index for estimation of the nutritional state.

과학 영재 프로그램을 통해 본 과학 관련 태도와 과학 불안도의 관계와 변화

양태연,박상우,박인호,한기순 한국과학교육학회 2005 한국과학교육학회지 Vol.25 No.2

본 연구는 과학 영재 학생의 과학 관련 태도와 과학 불안도와의 관계가 어떠한 상관을 보이고 있고, 과학 관련 태도와 과학 불안도가 과학 영재 프로그램에 참여하기 전과 참여한 후에 어떻게 변화하는지를 보았다. 이에 따라 과학 영재 교육 프로그램에 참여하고 있는 148명의 중학생들을(남학생 : 95명, 여학생 53명)대상으로 과학 관련 태도 검사와 과학 불안도 검사를 실시하였다. 연구결과, 과학 관련 태도와 과학 불안도의 관계는 사전 검사에서 뚜렷한 부적 상관을 보였으나 사후 검사에서는 부분적으로 정적 상관이 나타났고 영재 프로그램 참여 후 과학 영재들의 과학 관련 태도는 낮아졌고 과학 불안도는 증가하였다. 이 연구의 흥미로운 점은, 여러 선행 연구결과와는 달리 영재 프로그램에 참여한 후 남학생이 여학생보다 과학 관련 태도가 낮아졌고 과학 불안도가 증가하였다는 점이다. This study examined scientifically gifted students' science attitude, anxiety, and their relationship. In addition, sex differences in these variables were investigated. The main interest of the study, however, was to explore how scientifically gifted students' science attitude and anxiety have changed before and after a gifted education experience. One hundred and forty-eight middle school students (95 boys and 53 girls) who enrolled in a gifted education program participated in the present study. The results indicate an increase in science anxiety and a decrease in science attitude after the six-month gifted program experience. Negative correlations between science attitude and anxiety were found in pre-test, but some positive relationship existed in post-test. Interestingly, after the specialized gifted program, male students' science attitudes were lowered significantly. In contrast, there was no statistically significant difference in female students. Results of the study were discussed in relation to the programming issues for the scientifically gifted.

임상실습 교육을 위한 임상실습지도자제도(preceptorship)의 모형개발

안양희,최상순,허혜경,임영미,신윤희,박소미,김대란,김기경,김기연 연세대학교 교육대학원 2002 연세교육과학 Vol.50 No.-

The purpose of this study was to construct a modified preceptorship model that could be used to improve the quality of clinical practice for student nurses in the Department of Nursing, Wonju College of Medicine, Yonsei University. Through a literature review, three workshops, and analysis of the preceptorship performance of new nurses, three findings were identified. First, based on the literature review and workshops, a modified preceptorship proposal for clinical practice for student nurses in the hospital setting was developed. It contained standard criteria outlining qualifications and rewards for preceptors, training for preceptors, and regulations concerning reciprocal relations among preceptors, head nurses, clinical instructors, and students. Second, results of the analysis of the preceptorship performance of new nurses in the hospital found implications for improvement of self-esteem and self-confidence and, for burden in overloading. Third, based on the literature review, the modified preceptorship proposal, and the results of the analysis of the preceptorship performance of new nurses, a modified preceptorship model was constructed. The modified preceptorship model for clinical practice for student nurses in both hospital and community settings contained standard criteria outlining qualifications and rewards for preceptors, training and education fur preceptors, and regulations concerning reciprocal relations. The standard criteria for qualification as a preceptor should be at least a BSN, at least two years experience, and leadership ability. Rewards for preceptors could be job conditions. For the training and education of preceptors, the difference between hospital and community settings was the content and duration of training. The common training content for preceptors were objectives, orientation to curriculum and preceptorship, clinical practice guidelines, communication channels between health teams and preceptors, nursing process, nursing interventions, and evaluation. In conclusion, the findings suggest a need fur further study on preceptorship when used to improve the quality of clinical practice for student nurses in the both hospital and community settings. The ultimate goal of the research on preceptorship was not only to help student nurses to enhance their ability in clinical practice, but also to maintain the quality of clinical practice in the professional nurses in any clinical setting.

3차원 비디오 자동 동작분석 시스템 개발

박성순,양창수,장준원,이경일,우철호,백승국 한국운동역학회 1998 한국운동역학회지 Vol.8 No.2

The purpose of this study was to develope both hardware and software of automatic 3-dimensional video motion analysis system. In this image capture system, the vedeo image was stored as a file in CD-Rom directly, the refore it was possible to analyze motion without VCR. Reflective markers were used to digitize automaticaly. C-language and MS-Visual Basic 5.0 ] anguage were utiliged in this software to analyze motion. The conclusions were as follows; 1. The vedeo information at sixty frames per second could be captured accurately as a image with a resolution photography of 640×480-24 bit. 2. It was possible to digitize both manually and automatically utilizing reflective marks attached the body. 3. The automatic 3-dimensional video motion analysis system, which was developed in this study not only increased accuracy but also reduced digitizing time so much.