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Sung, C.G.,Kim, T.W.,Park, Y.G.,Kang, S.G.,Inaba, K.,Shiba, K.,Choi, T.S.,Moon, S.D.,Litvin, S.,Lee, K.T.,Lee, J.S. Elsevier 2014 Journal of marine systems Vol.137 No.-
Since the Industrial Revolution, rising atmospheric CO<SUB>2</SUB> concentration has driven an increase in the partial pressure of CO<SUB>2</SUB> in seawater (pCO<SUB>2</SUB>), thus lowering ocean pH. We examined the separate effects of exposure of gametes to elevated pCO<SUB>2</SUB> and low pH on fertilization success of the sea urchin Strongylocentrotus nudus. Sperm and eggs were independently exposed to seawater with pCO<SUB>2</SUB> levels ranging from 380 (pH7.96-8.3) to 6000ppmv (pH7.15-7.20). When sperm were exposed, fertilization rate decreased drastically with increased pCO<SUB>2</SUB>, even at a concentration of 450ppmv (pH range: 7.94 to 7.96). Conversely, fertilization of Hemicentrotus pulcherrimus was not significantly changed even when sperm was exposed to pCO2 concentrations as high as 750ppmv. Exposure of S. nudus eggs to seawater with high pCO<SUB>2</SUB> did not affect fertilization success, suggesting that the effect of increased pCO<SUB>2</SUB> on sperm is responsible for reduced fertilization success. Surprisingly, this result was not related to sperm motility, which was insensitive to pCO<SUB>2</SUB>. When seawater was acidified using HCl, leaving pCO<SUB>2</SUB> constant, fertilization success in S. nudus remained high (>80%) until pH decreased to 7.3. While further studies are required to elucidate the physiological mechanism by which elevated pCO<SUB>2</SUB> impairs sperm and reduces S. nudus fertilization, this study suggests that in the foreseeable future, sea urchin survival may be threatened due to lower fertilization success driven by elevated pCO<SUB>2</SUB> rather than by decreased pH in seawater.
PCR 기법을 이용한 한국재래산양의 α s2-casein 유전자의 특성
이상훈,상병찬,한성욱,김지애,서길웅 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4
This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from the analysis of genetic characteristics of α_(S2-) casein gene in Korean Native goat using PCR-RFLP. This study confirmed the amplified product of 1.3kb fragment obtained from the amplification of a α_(S2-)casein loci by PCR. The frequency of a α_(S2-)casein genotype in Korean Native goat was 100% for AA type and the frequencies of a α_(S2-)casein genotype in Saanen were 80 and 20% for AA and AB genotypes. The frequency of a α_(S2-)casein A allele was 1.000 in Korean Native goat, and the frequencies of a α_(S2-)casein A and B alleles were 0.900 and 0.100 in Saanen, respectively. The nucleotide sequence of a α_(S2-)casein gene of Korean Native goat was 99.0% homology with 12 nucleotide sequences difference of that of goat reported in GeneBank(AJ238965.1, AJ238967.1) and was 95.0% homology with 57 nucleotide sequences difference of that of Holstein reported in Gene Bank(M94327). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphisms and sequences for a α_(S2-)casein gene should be used as basic data for preservation and improvement of genetic resources in Korean Native goat breeding.
볏짚 및 가공처리 왕겨의 급여가 한우의 사료섭취 및 반추행동에 미치는 영향
이왕식,이병석,이상철,이상석,이세영,이덕윤,하종규 한국동물자원과학회 2004 한국축산학회지 Vol.46 No.1
Effects of low quality roughage sources on duration and frequencies of rumination and chewing in Hanwoo steers were determined. Animals were fed three diets; concentrate+rice straw)50:50), concentrate+rice straw+popped rice hull(50:35:15) and concentrate)+rice straw+ground rice hull(50:35:15) to compare both rice straw alone and combination with rice hull. Eating and ruminating time of steers fed concentrate+rice straw(50:50), concentrate+rice straw+popped rice hull(50:35:15) and concentrate+rice straw+ground rice hull(50:35:15) were 78.8 and 338.4min/day; 98.0 and 362.5min/day, and 160 and 519.2min/day, respectively. When steers were fed popped rice hull and ground rice hull, time spent for both eating and ruminating was significantly increased(p<0.05). When steers fed popped and ground rice hull, number of ruminating chews and number of chews per rumination time were significantly decreased(p<0.05). The number of rumination boli and boli per rumination time had significantly decreased(p<0.05) in steers fed both types of rice hull. In summary, when cattle were fed experimental diets substituted with popped or ground rice hull to rice straw, the rumination behaviors were increased, because popped or ground rice hull might physically stimulate rumen wall. Therefore, both the physically shape and size of roughage sources are important factors to induce rumination behavior.
이창규,이승관,조경진,박종성,정수경,유병서,박상숙,류정록,남현철,김석수,김상섭,김재영,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 1998 保健科學論集 Vol.24 No.1
Since the Korean Association of Quality Assurance for Clinical Laboratory was established in 1976, the numbers of the participating hospitals nowadays were increased from 40 to 450 or more hospitals all over the country. In the early day the both internal and external quality control programs were started at the same time, however, the external QC programs prevailed over the internal ones putting the cart before the horse. The accuracy and the precision of the data are not satisfying in spite of the 20 years experiences of quality control programs. All the daily data should be reported after the validity is assured by the self-evaluation not by third party evaluation. It is regret that the CVs of enzymatic tests in external QC are exceeding 10% and there is no sign of diminishing CVs. One of the major reasons is that the types of anaytical instrurments and reagents are diverisified. Moreover, most of them are subject to the standards of the respective country of manufacturer. Accordingly, we can make a suggestion that the reagents and instruments should be imported or supplied meeting the established requirements for Korean standardization.
Sang Guk Lee,Hee Moon,Kwang Hyun Park,심왕근,M.S. balathanigaimani 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3
Beer lees precursor is chosen to prepare activated carbons having different physical and chemical properties. The beer lees-based activated carbons (BL-ACs) are characterized by N2 adsorption/desorption isotherms, adsorption energy distributions (AEDs), and X-ray photoelectric spectroscopy (XPS). Furthermore the electrochemical properties of the BL-ACs are assessed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and constant current charge/discharge method. The maximum specific capacitance (about 188 F/g at discharge current of l mA/cm2) is obtained in 0.1 M H2SO4 electrolyte solution, which is a relatively low electrolyte concentration. The overall results suggest that the BL-ACs are good candidates for EDLC electrode materials.
Hereditary nonpolyposis colorectal cancer in endometrial cancer patients
Yoon, Sang Nam,Ku, Ja-Lok,Shin, Young-Kyoung,Kim, Kyung-Hee,Choi, Jin-Sung,Jang, Eun-Ja,Park, Hyoung-Chul,Kim, Duck-Woo,Kim, Min A.,Kim, Woo Ho,Lee, Taek Sang,Kim, Jae Weon,Park, Noh-Hyun,Song, Yong-S Wiley Subscription Services, Inc., A Wiley Company 2008 International journal of cancer: Journal internati Vol.122 No.5
<P>Endometrial cancer is the second most common cancer in hereditary nonpolyposis colorectal cancer (HNPCC). It has often been overlooked to explore the possibility of HNPCC in endometrial cancer patients. Our study was to investigate how many HNPCC patients existed among endometrial cancer patients. Among patients who underwent hysterectomy for endometrial cancer at Seoul National University Hospital from 1996 to 2004, 113 patients were included, whose family history and clinical data could be obtained and tumor specimens were available for microsatellite instability (MSI) testing and immunohistochemical (IHC) staining of MLH1, MSH2 and MSH6 proteins. There were 4 (3.5%) clinical HNPCC patients fulfilling the Amsterdam criteria II, and 2 (2/4, 50%) of them carried MSH2 germline mutations. There were also 8 (7.1%) suspected HNPCC (s-HNPCC) patients fulfilling the revised criteria for s-HNPCC, and one (1/8, 12.5%) of them revealed MLH1 germline mutation. In 101 patients, who were not clinical HNPCC or s-HNPCC, 11 patients showed both MSI-high and loss of expression of MLH1, MSH2 or MSH6 proteins, and 2 (2/11, 18.2%) of them showed MSH6 germline mutations. In 113 patients with endometrial cancer, we could find 5 (4.4%) HNPCC patients with MMR germline mutation and 2 (1.8%) clinical HNPCC patients without identified MMR gene mutation. Family history was critical in detecting 3 HNPCC patients with MMR germline mutation, and MSI testing with IHC staining for MLH1, MSH2 and MSH6 proteins was needed in the diagnosis of 2 HNPCC patients who were not clinical HNPCC or s-HNPCC, especially for MSH6 germline mutation. © 2007 Wiley-Liss, Inc.</P>
Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats
Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2
Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*