학술발표회 : 단위영양 ( 포스터발표 ) ; 지방첨가 사료급여가 제주재래마의 육생산 및 육성분에 미치는 영향

이왕식,진신흠,강수원,오운용,고서봉,김용곤,김규일 한국동물자원과학회 ( 구 한국축산학회 ) 1994 한국축산학회 심포지움자료집 Vol.- No.-

영양적 방법에 의한 곰팡이 독소의 피해 저감

이왕식,Lee, Wang-Sik 한국단미사료협회 2004 사료산업 Vol.2 No.10

사료중에 존재하는 위해요소로서, 곰팡이 독소는 아스퍼질러스, 페니실리움, 푸사리움속에 속하는 곰팡이의 2차 대사산물이며, 사람과 가축에 잠재적 독성이 있는 300여 가지의 곰팡이 대사산물이 발견되어지고 있다.(중략)

Saccharomyces sake KBA No . 6 에 의한 Ergosterol 의 생산

이왕식,방원기,박장우 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.1

To produce ergosterol Saccharomyces sake KBA No. 6 was used and various factors related to ergosterol accumulation were investigated. The most effective inorganic nitrogen source was ammonium chloride and 3.50 % of ergosterol was accumulated in the cell when the C/N ratio was 200/1. When Tween 80 and potassium nitrite were used simultaneously, ergosterol content and total amount of ergosterol were increased by 56 % and 45 %, respectively, compared to their control in which 0.2 % of Tween 80 and 0.1 % of potassium nitrite were used. Under the optimum conditions, ergosterol content increased from 1.73 % to 5.3 % and the total amount of ergosterol was increased from 65.2 ㎎/1 to 135.15 ㎎/1.

Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus

이왕식,안준형,정상희,이재종,강태준,정진영 한국바이오칩학회 2021 BioChip Journal Vol.15 No.3

Since the first emergence of influenza viruses, they have caused the flu seasonally worldwide. Precise detection of influenza viruses is required to prevent the spreading of the disease. Herein, we developed an optical biosensor using peptide-immobilized nanopillar structures for the label-free detection of influenza viruses. The spin-on-glass nanopillar structures were fabricated by nanoimprint lithography. A sialic acid-mimic peptide, which can specifically bind to hemagglutinin on the surface of the influenza virus, was immobilized onto the nanopillars via polymerized dopamine. The constructed nanopillar sensor enabled us to detect influenza A viruses in the range of 10 3 –10 5 plaque-forming units through simple measurements of reflectance. Our findings suggest that biomimetic modification of nanopillar structures can be an alternative method for the immunodiagnosis of influenza viruses.

Rhizobium Japonicum 1 - 23 Glutamine Synthetase Ⅰ의 분리 정제 및 성질

이왕식,성하진,방원기 ( Wang Sik Lee,Ha Chin Sung,Won Gi Bang ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.4

Glutamine synthetase I (GS I) was induced in Rhizobium japonicum 1-23 grown on glycerol as a sole source of carbon. The enzyme was purified 28.8 fold by heat-treatment ammonium sulfate fractionation, DEAE-Sepharose ion-exchange chromatography, Cibacron-Blue F3GA affinity chromatography, and Ultrogel AcA 22 gel fractionation. The n and the isoactivity point of GS I were 4.5 and 7.53, respectively. Molecular weight of GS I was 600,000 composed of twelve identical 52,000 subunits. The activities of GS I were inhibited by 87.2% (7.14 mM of alanine) and 98.1% (5 mM of inosine). K_m for the substrate, glutamine, was 5.4 mM and V_(max) was 6.90 μ㏖/min/㎎.

푸사리움속 곰팡이에 오염된 보리와 보리겨의 급여가 비육돈 및 육성빈돈의 생산성 및 사료이용효율에 미치는 영향

이왕식,이현준,기광석,노환국,강석진,정영훈,백광수,허태영,Lee, Wang-Shik,Lee, Hyun-June,Ki, Kwang-Seok,Noh, Hwan-Gook,Kang, Seok-Jin,Jung, Young-Hun,Baek, Kwang-Soo,Hur, Tai-Young 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.1

The present study was carried out to investigate the effect of barley and barley bran contaminated with Fusarium spp on growth performance and feed efficiency of fattening and growing pigs. In experiment 1, total 48 fattening Landrace pigs were used in a fattening trial for 71 days. Pigs weighing around 75 kg were allocated into different substitution groups containing 0, 10, 20 and 30% of barley contaminated Fusarium spp. In experiment 2, total 16 growing Landrace pigs were used in a growing trial for 45 days. Pigs weighing around 29.4 kg were allocated into different substitution groups containing 0, 5, 10 and 20% of barley bran contaminated Fusarium spp. Mycotoxin concentrations of barley and barley bran contaminated with 30% Fusarium spp were 0.452 and 1.049 ppm for deoxynivalenol, 8.125 and 17.646 ppm for nivalenol and 0.023 and 0.029 ppm for zearalenone, respectively. In experiment 1, no differences were found in weight gain and feed intake between control group (0%) and 10 or 20% substitution groups, but in 30% substitution group, weight gain and feed intake were significantly lower (p < 0.05) than those in control group. After slaughtering, the extended haemorrhage of the fundus region in stomach was observed in 20 or 30% substitution groups. In experiment 2, weight gain and feed intake were not significantly different among treatment groups. After slaughtering of experimental pigs, the extended haemorrhage of the fundus region in stomach was observed in pigs fed diet with 20% substitution group. These results suggest that the feeding of diet with contaminated highly levels of Fusarium spp was negative effect on growth and feed efficiency in growing and fattening pig.

Bermudagrass와 Bahiagrass의 품종별 수확시기 및 파종방법에 따른 생산성 및 사료가치 비교

이왕식,임석주,김범준,김영진,김동훈,황경준,김시현,우제훈,박남건,Lee, Wang Shik,Im, Suk Ju,Kim, Bum Jun,Kim, Young Jin,Kim, Dong Hoon,Hwang, Kyung Jun,Kim, Si Hyun,Woo, Jae Hoon,Park, Nam Geon 한국초지조사료학회 2018 한국초지조사료학회지 Vol.38 No.4

본 연구는 제주지역에서 초식동물의 방목을 위해 주로 이용되고 있는 한지형 목초의 경우에 여름철 하고기에 생산성이 저하되고 있어 여름철에 생산성을 높일 수 있는 난지형 목초를 도입하여 이용하기 위한 목적으로 수행되었다. 시험에 사용된 난지형목초는 Bermudagrass 품종(Cynodon dactylon (L.)Pers. : Giant, Cheyenne, Mohawk, Panchero Frio, Common, Tifton 85)과 Bahiagrass 품종(Paspalum notatum : TifQuik, Tifton 9)을 이용하였으며, 해발 450m고지에서 월동 후에 수확시기별 생산성 및 영양소 함량의 변화를 조사하였다. 시험구는 품종별로 8처리 3반복 난괴법으로 배치하여 수행하였다. 파종방법은 산파와 조파형태로 파종되었으며, Tifton 85품종은 줄기 파종되었다. 시험결과를 보면 월동 후에 Bermudagrass와 Bahiagrass 품종 모두에서 동해에 의한 피해로 봄철에 재생이 대체로 늦었으며, 이중 줄기 파종되었던 Bermudagrass의 Tifton 85와 TifQuik, Tifton 9 품종이 다른 품종에 비해 동해에 비교적 강한 것으로 평가되었다. 생초와 건물수량은 품종에 따라 유의성 있는 차이를 보여 주었는데(p<0.05). Tifton 85, TifQuik 및 Tifton 9 품종의 생초수량이 다른 품종에 비하여 우수하였으며, 건물수량은 Tifton 85와 Tifton 9 품종이 다른 품종에 비하여 우수하였다. 건물 함량에 있어서도 품종에 따라서 유의있는 차이를 보여주었고(p<0.05), Giant 품종이 다른 품종에 비하여 높은 결과를 나타내었다. 수확시기별 건물 함량은 Bermudagrass의 모든 품종에서 수확시기에 따른 차이를 보였다(p<0.05). 조단백질 함량도 품종에 따라 유의있는 차이를 나타내었고(p<0.05), Cheyenne, Mohawk, Panchero Frio와 Common 품종이 다른 품종에 비하여 높은 결과를 보여주었으며, Giant 품종에서는 파종방법과 수확시기에 따른 차이를 나타내었다(p<0.05). 조섬유 함량도 품종에 따라 차이를 나타내었으며(p<0.05), Tifton9 품종이 다른 품종에 비하여 유의있게 높은 함량을 보여주었고, 수확시기별 조섬유 함량은 Mohawk과 Tifton 85 품종에서 유의있는 결과를 나타내었다(p<0.05). 그리고 조회분 함량에 있어서도 품종에 따른 유의있는 차이를 보였으며(p<0.05), Cheyenne이 다른 품종에 비하여 유의있게 높은 함량을 보였다. 수확시기별 조회분 함량은 Cheyenne, Panchero Frio와 Tifton 85 품종에서 유의있는 결과를 나타내었고(p<0.05), 파종방법에 따른 차이는 Giant 품종에서 유의있는 결과를 보였다(p<0.05). 이러한 결과에 따르면 난지형 목초류는 품종에 따라 동해에 따른 재생능력, 생산성, 영양소 함량이 차이가 크게 나타나고 있어 이용목적에 따라 선택할 필요가 있으며, 이용을 확대하기 위하여서는 다양한 해발고도에서의 평가가 필요한 것으로 사료되었다. The southern type grasses announced for the test were the Bermudagrass cultivars (Giant, Cheyenne, Mohawk, Panchero Frio, Common and Tifton 85) and the Bahiagrass cultivars (TifQuik, Tifton 9), and the changes in the productivity and nutrient content were surveyed in Jeju area (450m altitude). The different cultivars were sowed by broadcasting or drill seeding method, and Tifton 85 was transplanted from sprigs. The fresh and dry matter yield showed varying significant differences for different cultivars (p<0.05). The fresh yields of Tifton 85, TifQuik and Tifton 9 were excellent, compared to the other cultivars, and for the dry matter yield, Tifton 85 and Tifton 9 were excellent when compared to the other cultivars. Crude protein content showed significant differences among different cultivars (p<0.05). Cheyenne, Mohawk, Panchero Frio, and Common showed differences in the crude protein content by sowing method and harvest time (p<0.05). The different cultivars showed differences in the crude fiber content (p<0.05), and Tifton 9 registered significantly high content and Mohawk and Tifton 85 showed significant crude fiber content by harvest time (p<0.05). According to these results, the southern type grass cultivars showed big differences in the regenerative capacity against damage from frost, productivity, and nutrient content, so they need to be chosen according to the purposes, and to increase their usage, their evaluation needs to be conducted at various altitudes.

단위영양 ; 생균제 , 셀룰로스 또는 유당을 함유한 사료의 급여가 쥐의 장내 요소분해효소 활성 및 암모니아 생산에 미치는 영향

이왕식,김규일 한국동물자원과학회 ( 구 한국축산학회 ) 1993 한국축산학회 심포지움자료집 Vol.- No.-

볏짚 및 가공처리 왕겨의 급여가 한우의 사료섭취 및 반추행동에 미치는 영향

이왕식,이병석,이상철,이상석,이세영,이덕윤,하종규 한국동물자원과학회 2004 한국축산학회지 Vol.46 No.1

Effects of low quality roughage sources on duration and frequencies of rumination and chewing in Hanwoo steers were determined. Animals were fed three diets; concentrate+rice straw)50:50), concentrate+rice straw+popped rice hull(50:35:15) and concentrate)+rice straw+ground rice hull(50:35:15) to compare both rice straw alone and combination with rice hull. Eating and ruminating time of steers fed concentrate+rice straw(50:50), concentrate+rice straw+popped rice hull(50:35:15) and concentrate+rice straw+ground rice hull(50:35:15) were 78.8 and 338.4min/day; 98.0 and 362.5min/day, and 160 and 519.2min/day, respectively. When steers were fed popped rice hull and ground rice hull, time spent for both eating and ruminating was significantly increased(p<0.05). When steers fed popped and ground rice hull, number of ruminating chews and number of chews per rumination time were significantly decreased(p<0.05). The number of rumination boli and boli per rumination time had significantly decreased(p<0.05) in steers fed both types of rice hull. In summary, when cattle were fed experimental diets substituted with popped or ground rice hull to rice straw, the rumination behaviors were increased, because popped or ground rice hull might physically stimulate rumen wall. Therefore, both the physically shape and size of roughage sources are important factors to induce rumination behavior.

농후사료와 조사료의 비율이 한우의 저작 및 반추시간과 빈도에 미치는 영향

이왕식,이병석,오영균,김경훈,강수원,이상석,하종규 한국동물자원과학회 2004 한국축산학회지 Vol.46 No.1

The effects of concentrate to roughage ratios on duration and frequencies of rumination and chewing in Hanwoo steers were determined. Five Hanwoo steers fitted with rumen fistula were used to evaluate the effects of concentrate to rice straw ratio on eating and ruminating behavior. Experimental diets were formulated with different concentrate to rice straw ratios(50:50, 60:40, 70:30, 80:20, 90:10). When level of roughage feed was increased by 10, 20, 30, 40 to 50% of total dry matter of the diet, total chewing time was increased linearly from 286.99, 321.09, 390.29, 406.63 to 423.30 min/d, which was mainly due to increased ruminating time from 204.91 to 342.80 min/day. However, the level of roughage did not affect eating time. The number of chews per day for rumination and number of chews per rumination were significantly decreased(p<0.05) as roughage level was increased. In summary, the duration and frequencies of rumination in Hanwoo increased with increased rice straw level.