건강인에서 결핵균 30-kDa항원이 말초혈액 임파구증식에 미치는 영향

최대경,고석신,김화중,조은경,백태현,신철식 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

T lymphocytes are thought to play a central role in cell mediated immune response. To Study the T lymphocyte proliferative response to 30-kDa antigen purified from Mycobacterium tuberculosis H37Rv, peripheral blood lymphocytes isolated from healthy subjects were stimulated with the 30kDa antigen, crude antigen or PHA. Healthy subjects were divided into three groups so that PPD(+), PPD(±) and PPD(-). The proliferations of lymphocytes were measured by the incroporation of ^3H-thyrnidine. The results were summarized as follows: 1. For the PPD(+) person, at concentration of < 1 ㎍/ml, the proliferative response to 30-kDa antigen resulted in a mild reduction but crude antigen showed relatively rapid reduction. For the PPD(-) person, decreasing the concentration of the both antigens showed rapid reduction of proliferation 2. The lymphocytes proliferations to 30-kDa antigen and crude antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group, but PHA response was no significant difference. 3. The sensitivity and specificity of lymphocyte proliferation assay to 30-kDa antigen and crude antigen when compared with skin test results were 92.3%, 100% and 76.9%, 50%, respectively.

흰쥐에서의 Puromycin Aminonucleoside-유발 단백뇨에 대한 선택적 Thromboxane A₂수용체길항제, KT2-962의 효과

서대규,신인철,고현철,하경란,강주섭 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.1

The administration of puromycin aminonucleoside(PAN) to rats caused to nephrotic syndrome which characterized ascites, proteinurisa, hypoalbuminemia, and hyperlipidemia similar to those observed in human minimal change disease. Recently, several studies indicate that renal endogenous thromboxane(Tx) A₂may have an important role in pathophysiology of various renal disease. In this sutdy, we hafve examined the protective effct of a selective TxA₂receptor antagonist, KT2-962(KT2) on PAN-induced proteinuria in rats. Thus, male Wistar rats were given either daily subsutaneous injection of PAN, 20mg/kg, for 10 consecutive days from 3 days before to 7 days with PAN treatment. Urine was collectd, and body weight was measured in interval of 2 days during 2 weeks and urinary N-acetyl-β-Dglucosaminidase(NAG) activity as an index of renal tubular cell damage and urine protein were measured. In addition to measuring BUN, serum creatinine and creatinine clearance were measured to assess the degree of renal functional damage in 14th day. The results(Means SE) otained can be summarized as follows: 1)Body weight(gm) was progressively increased and gained about 46.4gm and 39.2gm on 2 weeks of treatment in the control and KT2 groups respectively. In constrast, there was weight loss about 27.4gm in the PAN group. But, it was increased about 23.2 gm in KT2+PAN grou and means that KT2 has significantly(p<0.05) suppressed weight loss by PAN. 2)Urine flow (ml/24 hours) was slightly increased in both control and KT2 groups during 2 weeks. But, it was significantly(p<0.05) increased after 7th groups during 2 weeks. But, it was significantly(p<0.05) increased after 7th day. But, concurrent administration of KT2 significantly(P<0.05) suppressed PAN-induced polyuria in KT2+PAN group. 3)Urinary protein(mg/24 hours0 was slightly increased in both control and KT2 groups. But, it was progressively increased and reached at the maximal level, 3.2 folds of initial level to 11th day and thereafter slightly reduced proteinuria to 14th day in the PAN group. In contrast, KT2 cotreatment with PAN was significantly(P<0.05) suppressed PAN-induced proteinuria in the KT2+PAN group. 4)Urinary NAG activity was markedly increased and reached to maximal level, 122.03 18.53 U/mg of urine creatinine, 12.7 folds of initial by day 9 and thenafter progressively decreased to 5.4 folds of initial level by day 14 in the PAN group. But, when KT2 was administered with PAN, it was significantly depressed its increment to day 13. But, it was reached to maximal level, 99.05 42.55, 12.7 folds of inital level much than PAN group. This result indicated that KT2 had a partial preventive effect on PAn-induced renal tubular cell damage. 5)The BUN and serum creatinine level(mg/dl) were significantly(p<0.05) increased from initial level, 18.48 1.28 and 0.50 0.03 to 118.42 41.34 and 1.66 0.27 respectively, and creatinine clearance(ml/min) was significantly(P<0.05) decreased from initial level, 0.44 0.02 to 0.28 0.07 by day 14 by PAN treatment. But, when PAN was given together with KT2, the increment of BUN and serum creatinine except for creatinine clearance were significantly(P<0.05) inhibited in the KT2+PAN group. Based on all these results obtained in this study, it is concluded that the coadministration of KT2-962 with PAN can be ingibited protein excretion in urine and suggested that endogenous TxA₂would take part in PAN-induced proteinuria in rats.

各種 食品에서 分離한 眞菌 培養液으로 處理된 마우스 肝細胞의 電子顯微鏡學적 觀察 : Ⅰ. 數種 mycotoxin 分泌菌株(Reference株)에 對한 成績 Ⅰ. Results of Several Reference Strains

등영건,高春明,金聖光,孫瑀鍾,崔大卿 최신의학사 1974 最新醫學 Vol.17 No.4

The purpose of present study is to determine the toxicity of several mycotoxin-producing fungi, named A. flavus ATCC 15517, A. parasiticus RIB 1037, A. toxicarium RIB 4002, P. citrinum SWU 238, P. islandicum IFO 5235, P. tardum IFO 5787, and P. brunneum by observing the ultrastructural changes in the mouse liver cells. The results as follows: 1. All of the experimental animals treated with reference strains were observed the focal necrosis and inflammatory infiltration of liver parenchymal cell. 2. It showed cytoplamic changes such as dilatation of endoplasmic reticulum, swelling of mitochondria, detachment of ribosomes, increased number of lipid droplets and glycogens. 3. Nuclear and nucleolar alterations were also noted the nucleolar capping, segregation of nucleolar elements and irregularity of nuclear envelopes.

부직포활성슬러지법에 의한 하수와 침출수처리에 관한 기초연구

정유진,고현웅,김경순,윤태경,성낙창 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

In this study, it was performed using submerged nonwoven bioreactor(SNBR) for removal of organic matter, nitrogen and phosphate under different aeration intervals(intermittent aeration). We applied the SNBR at the cheap nonwoven fiber module instead of the expensive membrane. The SNBR was mainly made up of an activated sludge reactor and a transverse flow nonwoven module. with an innovative configuration being in application between them. In case of sewage, the aeration conditions experimented consist of continuous aeration and 60min/60min, 120min/60min, 120min/120min of aeration/nonaeration time intervals, respectively. In case of landfill leachate, the intermittent aeration condition was 120min/120min at aeration/nonaeration. Consequently. a high COD removal rate (about 94%) was achieved in sewage and leachate. Although nutrient removal rate was relatively high without any additional chemicals.

좌심실 수축기 기능장애를 동반한 양수색전증 1예

박대균,홍경순,두영철,한규록,오동진,유규형,임종윤,고영박,이광학,이영,송원근,김현태 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.8

초등학교 과학 수업에서 개념도 활용의 효과

강석진,이유영,고한중,전경문,노태희 한국초등과학교육학회 2004 초등과학교육 Vol.23 No.1

In this study, we investigated the effects of using concept mapping as an instructional tool on 4th graders' achievement, science learning motivation, and attitude toward science classes. T재 classes (38 students) from an elementary school were respectively assigned to a control group and a treatment group. Students were taught about "expansion of matter by heat" and "heat transfer". These topics were chosen because they require students to understand more concepts as well as relationships among them. A science learning motivation test and an attitude toward science classes test were administered as pretests. A researcher-made achievement test, the science learning motivation test, and the attitude toward science classes test were administered as posttests. The results indicated that using concept mapping in 4th-grade science classes was not significantly effective in improving students' achievement though a statistically significant positive effect was found in the subcategory of knowledge. No statistically significant effect of using concept mapping was found in the scores of the science learning motivation test and the attitude toward science classes test. Educational implications are discussed.

자기공명 분석법을 이용한 백서 피판의 대사에 대한 연구

이윤,이대근,임태환,윤근철,고경석,이춘신,정복성 대한성형외과학회 1992 Archives of Plastic Surgery Vol.19 No.3

Innumerable techniques have been used to measure, index, or predict the viability of skin flaps after elevation. However, these are largely indirect and don't accurately reflect the events that occur at kthe cellular level. A method was sought that gives direct, reproducible, and accurate data about physiological and biochemical changes that occur during flap elevation. Because of its ability to monitor changes in the levels of high energy phosphorus metabolites(ATP, or adenosine triphosphate, PC or phosphocreatine, Pi or inorganic phosphate), 31-P magnetic resonance spectroscopy (MRS) holds promise of providing direct assessment of the metabolic status and biochemical changes that occur during skin flap elevation. MRS monitoring was performed on three portions of raised random pattern skin flaps of 5 rats. The spectrometer used for 31-P nuclear magnetic resonance analysis was a Bruker Biospec(4.7 Tesla) system, and a modified surface coil constructed of two layers of copper coil was used. The results have validated the ability of 31-P MRS to : 1. define the regional flxes in the levels of ATP, PCr, and Pi in skin flaps. 2. demonstrate that, of these, PCr levels represent the most sensitive indicator of flap well being. 3. measure intracellular pH through the chemical shift of the Pi resonance. 4. establish predictive correlations between profile of normal, compromised, and failing skin flaps and have thus prepared the way for noninvasive in vivo topical magnetic resonance investigations of flaps.

사상체질(四象體質)과 성별(性別)에 따른 위염(胃炎)의 정도, 종류 및 소화기(消化器) 증상(症狀)에 관한 연구

이태규,황민우,임진희,김상복,이수경,고병희,송일병,Lee, Tae-Gyu,Hwang, Min-Woo,Lim, Jinny,Kim, Sang-Bok,Lee, Soo-Kyung,Koh, Byung-Hee,Song, Il-Byung 사상체질의학회 2004 사상체질의학회지 Vol.16 No.2

1. Objectives However there was a report that dyspepsia occurs more frequently in Soeumin, there has not been any clinical research if structural changes such as gastritis occurs differently according to Sasang Constitution. So in this research, we investigated to find out if there are differences in the degree and types of gastritis and gastrointestinal symptoms according to Sasang Constitution and gender difference. 2. Methods Subjects were 30 men and 38 women, who had visited Kyung-Hee Medical Center for physical examination and took gastroscopy and differential diagnosis of Sasang Constitution in March, 2003. We investigated the degree and types of gastritis and gastrointestinal symptoms. 3. Results and conclusions Gender difference han no relation with types of gastritis, but had significant relation with the degree of gastritis and gastrointestinal symptoms. But Sasang Constitution had no significant relation with the degree and types of gastritis and gastrointestinal symptoms.

중풍입원환자의 체질별 소증(素證)과 중풍 발생 후 병증(病證)에 관한 임상적 연구

함통일,황민우,이태규,김상복,이수경,고병희,송일병,Ham, Tong-Il,Hwang, Min-Woo,Lee, Tae-Kyoo,Kim, Sang-Bok,Lee, Soo-Kyung,Koh, Byung-Hee,Song, Il-Byung 사상체질의학회 2004 사상체질의학회지 Vol.16 No.3

1. Objectives The purpose of this study is to investigate constitutional ordinary and pathological symptoms differences among each constitutional groups of stroke in-patients. 2. Methods 101 stroke in-patients(Soyangin(SY) $n^{a)}=52$, Taeumin(TE) n=39, Soeumin(SE) n=10) hospitalized at Kyung-Hee Oriental medical center from Nov. 2003 to Sept. 2004 were investigated through questionnaire which consists of 18 parts, 289 questions(156 questions concerned ordinary symptoms and 133 pathological symptoms). The answers were analyzed statistically in order to find the questions which represent the significant differences among each constitutional groups. ( a) number of patients) 3. Results As for the ordinary symptoms, majority of the Soyangin group replied that they experienced "frequent dreams during sleep" and "scarce constipation". Majority of the Taeumin group affirmed to "dark redness of face", "dry nose", "low intolerance of heat", "good appetite and digestion", and "frequent overeating experiences". Majority of the Soeumin group affirmed to "pale face", "insomnia", "frequent constipation", "poor appetite and digestion", "small amount of diet", "slow eating", "fond of warm or hot water", and "easily tired(especially after sweating)". As for the pathological symptoms, majority of the Taeumin group affirmed to "frequent optic fatigue", "frequent tinnitus", "frequent bitter mouth", "fond of cold water", "flatulent", "stuffiness in the navel area", "irregular stool habits", "frequent constipation", "large amount of sweat", and "frequent rigidity of neck". Majority of the Soeumin group affirmed to "bad appetite", "fond of warm or hot water", "drink small amount of water", "sensitive during sleep" and "frequent heavy head". 4. Conclusions Through this study, We found statistically significant questions distinguishing the characteristics of each constitutional group.

사상의학(四象醫學) 전문의(專門醫)의 체질진단(體質診斷)결과 일치도에 관한 연구

함통일,황민우,이태규,김상복,이수경,고병희,Hahm, Tong-Il,Hwang, Min-Woo,Lee, Tae-Gyu,Kim, Sang-Bok,Lee, Soo-Kyung,Koh, Byung-Hee 사상체질의학회 2006 사상체질의학회지 Vol.18 No.3

1. Objectives The purpose of this study was to investigate the concordance rate of the diagnostic results among Sasang Constitutional Medicine(SCM) specialists. 2. Methods We carried out two studies in order to investigate the concordance rate of the diagnostic results between SCM specialists. We calculated the kappa value for analyzing concordance rate(using the SPSS program). 1) Study A : We investigated 142 clients who had taken health examinations and diagnosis of Sasang constitution from March 9, 2005 to March 29, 2005 at a health examination center of Kyung-Hee medical center. Every SCM specialist diagnosed 142 clients directly. Each SCM specialist don’t know the other specialist’s diagnosis. 2) Study B : We investigated 176 clients who had taken health examinations and diagnosis of Sasang constitution from January 2005 to March 2005 at the department of Occupational and Environmental medicine of Kyung-Hee medical center. One SCM specialist diagnosed 176 clients directly and the other specialist knowing previous specialist’s diagnosis, diagnosed each person indirectly 3. Results 1) The concordance rate of the diagnostic results among SCM specialists in study A was relatively high(kappa value=0.700). 2) The concordance rate of the diagnostic results among SCM specialists in study B was high(kappa value=0.895). 4. Conclusions We can confirm the concordance rate of the diagnostic results among SCM specialists is relatively high.