인체 유방암종에서 Cyclic-Dependent Kinase Inhibitor, p27^Kip1 발현의 의의

송상용,김덕환,서연림,고영혜,김대식,남석진,양정현 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.12

Dabsyl Chlorides의 親核性 置換反應

成大東,朴現錫,柳俊夏,金良姬,李鍾八,嚴泰燮 東亞大學校 1990 東亞論叢 Vol.27 No.1

Kinetic studies of nuclsophilic substitution reaction of dabsyl chloride with pyridines have been investigated at 30℃ in a range of methanol-acetonitrile bianary solvent mixtures. The order of magnitude fo reactivity is β-picoline > pyridine > 1.5-N D A > α-picoline to react with dabsyl chloride, especially in case of α-picoline, the reactivity revealed lower than others, it is suggestd that nitrogen atom of pyfidine ring has a steric hinderance by the neightboring methyl group. The value of ρ(-0.96∼4.59) and β(0.36∼0.67) associated with a change of substituent in the nucleophile are large and indicate a realtively advanced bond formation in the transition state. Solvatochraomic correlations were predicted the importance of bond formation transition state, showing a greater contribution of polaritypolarizability (π*) lone paired to hydro-gen bond donar acidity (α). We conclude that the reaction of dabsyl chloride with pyridine proceed via S2 type reaction mechanism, as well as the reaction of dansyl chloride pyridine.

자궁경부암에서 Glutathione S Transferase-π의 분포에 관한 연구

양승하,김의한,강영근,김대중,강상균 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Uterine cervical carcinoma is the most common malignant tumor of woman in korea. GST-π is multifunctional and is implicated to detoxication of drug and carcinogenesis. This enzyme is expressed in many tumors of human. Relationship between GST-π and drug resistence on uterine cervical carcinoma is not known, but condyloma accuminatum, CIN and invasive carcinoma express this isoenzyme, and it is regarded as a useful marker in preneoplasia and neoplasia. To observe the expression of GST-π in CIN and invasive carcinoma, we analyzed CIN 26 cases and invasive squamous cell carcinoma 61 cases by immunohistochemical stain using monoclonal mouse anti-human GST-π. The results are as followings: 1) The GST-π showed positive reactionin normal and metaplastic epthelium adjacent to CIN and invasive carcinoma in forty-three cases(62.3%) of sixty-nine cases. 2) The GST-π showed positive reactionin CIN in eighteen cases(69.2%) of twenty-six cases. Positive rates of CIN Ⅰ, CIN Ⅱ and CIN Ⅲ are 66.7%, 71.5%, 68.8%, respectively. 3) The GST-π showed positive reaction in invasive squamous cell carcinoma in fifty-one cases(83.6%) of sixty-one cases. Positive rates of microinvasive and invasive squamous cell carcinoma are 84.6% and 83.3%, respectively. According to cell type, keratinizing and nonkeratinizing type are 81.8% and 84.6%, respectively. Above results revealed that GST-π may be significance as a immuohistochemical marker in CIN and invasive SCC, and may be implicated in intrinsic resistance for anticancer drug.

1-Di-butylaminonaphthalene-5-Sulfonyl Chloride와 Pyridine 置換體와의 反應

成大東,李鍾八,朴現錫,金良姬 東亞大學校 大學院 1989 大學院論文集 Vol.14 No.-

Nucleophilic substitution reaction of bansyl chloride with mathyl substituted pyridines have been studied kinetically at 0℃ in methanol-acetonitrile binary solvent mixtures. The order of magnitude of reactivity is γ-picoline>β-picoline>pyridine to react with bansyl chloride. The Bronsted βand Hammett ??values indicated that the bond formation is advanced more than the bond breaking at the transition state. The reactivity of nucleophiles is in accord with charge controlled and the reactivity of substrate has shown an orbital controlled reaction.

대장선암에서 p53과 c-erbB-2의 표출 및 상관관계에 관한 연구

양승하,강상균,김대중,김의한,김만성 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Colorectal adenocarcinoma is relatively common malignant tumor in korea and various oncogenes has found in tumor cells. Colorectal adenocarcinoma show overexpression of p53 and c-erbB-2. The relation between expression of p53 and c-erbB-2 and prognosis of patients in colorectal adenocarcinoma has studied recently, but that is controversy. To evaluate relation between expression of p53 and c-erbB-2 and histopathologic finding prognostic factors, we have performed immunohistochemical staining, using mouse anti-p53 and anti-human c-erbB-2, for 52 cases of colorectal adenocarcinoma. The results were as follows; 1) Expression rate of p53 and c-erbB-2 in colorectal adenocarcinoma were 71.2% and 51.9%, respectively. 2) Expression rate of p53 in left side(70.4%), differentiated(75.6%) and invasion to serosa and surrounding adipose tissue(72.5%) were higher than in right side(37.5%), poorly differnetiated(42.9%) and invasion to muscle(50%). 3) Expression rate of c-erbB-2 in left side(51.9%), differentiated(57.8%) and invasion to serosa and surrounding adipose tissue(54.5%) were higher than in right side(37.5%), poorly differnetiated(14.3%) and invasion to muscle(37.5%). 4) The expression of p53 and c-erbB-2 showed coexpression in colorectal adenocarcinoma. 5) The expression of p53 and c-erbB-2 showed no differenece according to presence or absence of lymphatic metastasis. Above result revealed that p53 and c-erbB-2 are coexpression in colorectal adenocarcinoma. The expression of each p53 and c-erbB-2 and the simultaneous expression of p53 and c-erbB-2 may be related to left side and differentiated cases. Our studies for relation with prognosis of patients in colorectal adenocarcinoma showed different results according to depth of invation and metastasis to lymph node and we should be followed for them.

폐장암에서 p53과 PCNA 표출에 관한 연구

김대중,양승하,정도영,강상균,김의한 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

To observe the expression of p53 protein and PCNA in lung carcinomas and to evaluate the correlationship between the two results, the author has been carried out immunohistochemical staining with mononuclear antibodies against p53 and PCNA. 64 cases of lung carcinomas used consist of 32 cases of squamous cell carcinomas, 18 cases of adenocarcinomas and 14 cases of small cell carcinomas, respectively. 1. p53 protein expression was seen in 16 cases out of 32 (50%) in squamous cell carcinoma, 7 cases out of 18 (39%) in adenocarcinoma and 10 cases out of 14 (71%) in small cell carcinoma. The positive rate of p53 is highest in small cell carcinoma. 2. p53 staining degrees showed that 3+ in 75% of squamous cell carcinoma, 1+ in 58% of adenocarcinoma and 1+ in 50% in small cell carcinma. were observed. The tumor cell composing of squamous cell carcinoma showed most strong staining to p53 protien. 3. PCNA labelling index showed higher index in p53(+) cases than p53(-) cases. The PCNA index of squamous cell carcinoma was highest and adenocarcinoma and small cell carcinoma in order. Above findings suggested that the PCNA index was related to p53 protein in tumor cells, but the PCNA index appeared to be influenced by the p53 protein content in tumor cells than whether the p53 protien was or not. So the growth rate of squamous cell carcinoma appeared to be most rapid than adenocarcinoma and small cell carcinoma.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

Paraquat lung에서 Fibronectin 분포에 관한 연구

강상균,양승하,김대중,김의한,김성용 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.1

To investigate the process of the Paraquat induced pulmonary fibrosis, 10 mg/Kg and 20mg/kg of paraquat was administered intraperitoneally each of 10 rats respectively. Three, five, seven, ten and fifteen days after this injection, the rats were sacrificed. Immunohistochemical studies for fibronectin and histologic studies were carried out. Results were as follows. 1. Toxic effect of the paraquat seems to be depend on the dose of administered paraquat since the groups of the rate treated with 10 mg/kg revaled no significant tissue damage after 7 days. 2. The pulmonary changes seen in 20 mg/Kg treated group could be classified into two stages: the early changes such as hemorrhage, edema and hyaline membrane formation which are similar to the findings observed in adult respiratory distress syndrome and the late change characterized by fibrosis which observed in mild degree even after 2 weeks, so the fibrosis seen to need more time for full blown. 3. The positivity of fibronectin were observed in macrophage and exudate in the early stage of tissue destruction that the fibronectin productin is preceded fibrosis and plays an important role for the collection of inflammatory cells such as macrophaques and fiboblasts. The more tissue desturction is severe, the more strong positivity of fibronectin was observed in the early stage of tissue destruciton.

위선암에서 p53, c-erbB-2, 그리고 DNA ploidy의 상관관계에 관한 연구

강상균,양승하,김대중,김의한,곽현수 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Gastric carcinoma is the most common malignant tumor in korea and many oncogenes have found in gastric carcinoma. The relation between the expression of p53 and c-erbB-2 and the prognosis of patient in gastric adenocarcinoma have studied recently, but it is controversy. To evaluate correlation between the pathologic findings including prognostic factors and the expression of p53 and c-erbB-2, and DNA ploidy, we have performed immunohistochemical staining using 83 cases adenocarcinoma and flow cytometry with 56 cases. The results were as follows; 1) The expression rate of p53 was 45.8% in gastric adenocarcinoma. Differentiated, intestinal, advanced, and lymph node metastatic cases showed higher expression rates than poorly differentiated, diffuse, and non-metastatic cases, without statistically significance. 2) The expression rate of c-erbB-2 was 62.6% in gastric adenocainoma. c-erbB-2 was related to differentiated and intestinal types. Advanced and metastatic cases showed higher expression rates of c-erbB-2 than early and non-metastatic cases, but there was not statistic significance 3) Gastric adenocarcinoma showed correlation of DNA diploidy with poorly differentiated type and aneuploidy with differentiated. 4) DNA diploidy and aneuploidy showed correlation with c-erbB-2 negative and postive cases respectively, but correlation between DNA ploidy with p53 expression was not proved. Above results showed that p53 and c-erbB-2 may be related poor prognosis but there was no statistic significance. DNA aneuploidy and diploidy showed correlation with c-erbB-2 expressed and non-expressed cases, respectively, but not with p53 expression.

하악측두관절에서 퇴행성 관절염의 실험모델 개발

신상훈,송동석,김철훈,황대석,김용덕,김욱규 부산대학교 병원 암연구소 2007 부산대병원학술지 Vol.- No.21

Purpose : The purpose of this study is to investigate temporomandibular joint arthritis that was induced by intra-articular injection of collagenase in rabbits. Material and Methods: In this study, 12 rabbis was used. We injected collagenase on temporomandibular joint of rabbit and observed for six weeks. H-E stain was done. Results : Four weeks later from 2.0 mg collagenase injection, we observe histologic appearance like arthritis. Conclusion : In our study using injection of collagenase in temporomandibular joint of rabbit we produce arthritis on temporomandibular joint of rabbit.