Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

PDP 격벽 금형 미세 홈 가공 특성 평가

김남훈,이은상,이득우,김남경,김덕환 한국공작기계학회 2003 한국생산제조학회지 Vol.12 No.4

This paper describes the machining characteristics of a developed micro grooving machine. Experiments have been conducted on the various grooving condition such as spindle revolution speed, feed rate and depth of groove. V and U-shaped blade tool and STD11 workpiece was used in this study. To evaluate the developed micro grooving machine, AE signal obtained from each experimental condition was analysed, and cutting stability was compared with the surface state. As a result, this study presented the process to optimize grooving condition and possibility of application of AE technique in groove machining.

圓光醫大 附屬 第2病院 神經精神科 患者에 對한 統計的 考察(Ⅱ)

박민철,장광철,김상원,김한주,유미경,김훈,유용진,김현정,박남진,오상우,이귀행,노승호,백영석 圓光大學校 醫科大學 神經精神科學敎室 1992 圓光精神醫學 Vol.8 No.1

본 연구는 1986년 7월 11일부터 1991년 7월 10일까지 원광의대 부속 제2병원 신경정신과에서 의래 및 입원치료를 받았던 6,072명의 환자를 대상으로 병원 역학조사를 실시하고 그 결과를 1차조사 (박민철 등1986)와 비교했다. 1.전체 환자 중 남자가 54.4%로 여자보다 많았고, 이리와 이리 인접지역이 84.0%로 대부분의 지역을 나타났다. 의료보장상태는 보험환자가 57.1%, 의료보호환자21.8%, 일반환자 21.1%였으며 연령별로는 20대 24.6%, 30대 22.8%로 20대 30대가 절반정도를 차지했다. 2.외래환자에서는 신경중성장애가 46.0%로 가장 많고 정동장애, 경련성장애 순이며, 입원환자에서는 정신분열증이 30.5%로 가장 많고 기질성정신장애, 정동장애의 순이었다. 3.남자는 신경증성쟝애(23.4%), 정신분열증, 기질성정신장애 순이고 여자는 신경증성장애(39.9%), 정동장애, 정신분열증의 순이었다. 남자가 여자보다 많은 진단은 알코올정신장애(94.6%), 기질성정신장애, 소아정신장애 순이고, 여자가 남자보다 많은 진단은 정동장애(64.5%), 신경증성장애였다. 4.진단별 연령분포에서 신경증성장에는 30대(25.3%), 20대, 40대 순이고, 정신분열증은 20대(40.5%), 30대, 40대 순이며, 정동장애는 20대, 30대, 50대 순이나 비교적 비슷한 융이었다. 5.진단별 발병연령에서 신경증성장에는 20대, 30대가 절반이고,정신분열증은 20대(51.3%), 10대, 30대 순이며 정동장애는 20대(30.7%), 30대, 50대 순이었다. 진단별 평균 발병연령은 신경증성장애 32.7세, 정동장애 36.1세, 정신분열증 23.6세였다. 6.진단별 이병기간은 2년이상이 신경증성장에 28.3%, 정신분열증 68.5% 정동장애 43.9%인데 전체적으로 41.0%였다. 진단별 평균 이병기간은 정신분열증 73.2개월, 정동장애 45.5개월, 신경증성장애 29.5개월이었다. We examined epidemiologic study of 6,072 patients who visited Wonkwang Neuropsychiatric hospital since July 11 of 1986 until July 10 of 1991 and compared this results with previous study(Park et al 1986). The study revealed as follows:1. Of total patients males visited this hosptial more than females as 57.1%, Most of them(84.0%) resided Iri and neighbouring Iri. In the point of insurance, insured patients, medicaid patients and uninsured patients were 57.1%, 21.8% and 21.1% respectively. On the distribution of age, the twenties, the thirties were 24.6%, 22.8% respectively and twenties and thirties were about half of total patients. 2. Of outpatients neurotic disorder was the most as 46.0%, secondly mood disorder and thirdly convulsive disorder, but of inpatients schizophrenia was the most as 30.5%, secondly organic mental disorder and thirdly mood disorder. 3. Of males neurotic disorder was the most as 23.4%, secondly schizophrenia, and thirdly organic mental disorder, but of females neurotic disorder was the most as 39.9%, secondly mood disorder and thirdly schizophrenia. alcoholic mental disorder(94.6%), organic mental disorder, child mental disorder were mostly found in males,but mood disorder(64.5%) and neurotic disorder were mostly found in females. 4. On the distribution of age neurotic disorder was found the most in the thirties(25.3%), next in the twenties and in the forties, and schizophrenia was found the most in the twenties(40.5%), next in the thirties and in the forties, but mood disorder was found the most in the twenties, next in the thirties and in the forties but revealed similar rates relatively. 5. On the distribution of age of onset neurotic disorder developed nearly half during the twrnties and the thirties, schizophrenia developed mostly during the twenties (51.3%), secondly the teens and thirdly the thirties, but mood disorder developed firstly the twenties(30.7%), secondly the thirties and thirdly the fifties. on the average age of onset of illness neurotic disorder, mood disorder and schizophrenia were 32.7 years, 36.1 years and 23.6 years respectively. 6. On the duration of illness more than two years was 28.3% in neurotic disorder, 68.5% in schizophrenia and 3.9% in mood disorder and on the average duration of illness schizophrenia, mood disorder and neurotic disorder were 73.2 months, 45.5 months and 29.5 months respectively.

閉塞性 換氣障碍 疾患에 있어서 動脈血가스 分析과 肺換氣機能 檢査에 關한 硏究

金獻男,崔仁善,朴炅玉 全南大醫大 1986 전남의대학술지 Vol.23 No.1

도심지 노후 상업 건축물의 리모델링과 재건축 비교 연구

김동훈,임남기 동명정보대학교 2002 건축도시연구소 저널 Vol.1 No.-

It we build new building after demolition of commercial building that is located in the downtown, it will be caused a social and environmental problem as wasting of resources and generating of waste. In this study, I investigated remodeling, this conclusion is given below. 1. As a result of comparing the cost of a construction wiht the term of works, it's represented that reconstruction cost is 2.1 times, construction period 1.4 times unfavorable to requirement and rental value 2.4 is time as much remodeling. So remodeling has an advantage. 2. For repairing and reinforcing timeworn building, we reinforced it as using carbon fiber sheet(girder, slab) and injecting method steel plate bonding method Also, we tried to maintain efficiency of new building as using epoxy to protect concrete crack. 3. In the period of demolition construction, a remodeling is reguired three times as much as a reconstruction, however, in the evacuation cost of one, the former is reguired more moderate price than the latter. The next remodeling in order to show and econmical perception of remodling to the owner of building, we have to consider percedent conditions as follows; the development of projects, and to partially clear up the difficult problem of raising money by developing a suitable organization as the merchandise of construction on the installment If we develop research with enterprise·university·laboratory to exploit material and equipment and to train specialized engineer who will has a capacity to know construct repair and reinforce, it can be attribute to prevail remodeling in new construct market.

이동경계조건을 고려한 유동해석기법

김남형,박지훈 제주대학교 공과대학 첨단기술연구소 2003 尖端技術硏究所論文集 Vol.14 No.1

In this paper, the numerical model of the flow analysis by finite element technique is described. The Galerkin method is employed for spatial discretization. Two step explicit finite element scheme is used to discretize the time function, which has advantage in problems treating large numbers of elements and unsteady state. Two dimensional hydrodynamic model considering moving boundary condition is developed. Also the model flume was applied to verify in the idealized water, and the results of this study confirm the efficiency of moving boundary treatment in coastal numerical computation.

一樣有界原理에 관한 槪觀

金憲男,黃洪澤 金烏工科大學校 産業技術開發硏究院 1999 産業技術開發硏究 Vol.15 No.-

1932년 바나하에 의해 소개된 一樣有界元理(Uniform boundedness Principle)는 베어 카테고리 定理를 이용하여 證明 되었으며, 대부분 문헌 역시 같은 방법으로 소개하고 있다. 본 논문은 베어카테고리 정리와는 관계없는 초보적인 보조정리를 이용한 一樣有界元理 證明을 소개 한다. 나아가 一樣有界元理를 응용하여 얻은 기존의 결과들을 재 조명함으로써, 동 원리를 응용할 수 있는 수학적 대상을 탐색하고 활용하는 데에 도움을 주고자 한다.

체외형 자동 제세동기(AED)와 심전도계(ECG)를 위한 통신 프로토콜의 설계 및 사용자 인터페이스 구현

김남훈,권희중,권호열,나학록 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A

In this paper, we studied Automated External Defibrillator and Electro- Cardiogram System. Proposed System, CU-EX1, is a PC-based AED/ECG system that manages the data acquired by Paramedic CU-ER1. The system controls the transfer of data from the Paramedic CU-ER1 and the storage of the data in a local host computer. Patient data could be entered as soon as the rescue data is stored in a local computer. The system also displays the data on a local computer and facilitates review of rescue events.

제주도 해수욕장의 자연조건에 관한 기초조사

김남형,장성훈 제주대학교 해양연구소 2000 해양자원연구소연구보고 Vol.24 No.-

Bathing beaches may be the one of the resorts in popular, which peoples can easily access in summer. Three beaches located in Jeju island are observed on natural conditions using coastal engineering technique. Also the satisfaction indexs of the sand size. wave height. water temperature. transparency and bottom slope are surveyed. The results obtained can be utilized making an artificial bathing beach.

소방대원들의 실내, 실외 근무 형태에 따른 유산소 운동능력 및 등속성 근력

김남익,장지훈,성기홍,윤성 한국유산소운동과학회 2002 대한스포츠융합학회지 (jcses) Vol.6 No.1

The purpose of study was to investigate the influence of cardiopulmonary function and isokinetic knee joint muscle strength on duty form in the fm fighters. 28men who aged 30-40 years were measured by resting pulmonary function test, exercise stress test(Q4500, Quinton, Co.) and isokinetic knee joint muscle strength testing(Cybex 6000 dynamometer system). Subjects were divided into the 2 groups that were indoor duty group and outdoor duty group. Statistical analysis was performed using analysis of variance t-test. Major findings and conclusion are as follows: In body fat test, outdoor duty groups was showed lower than that of indoor duty group. For the lung function test at rest, lung volume, vital capacity, MVV, FEVO.5-1.0, expiratory and inspiratory flow rate were checked. In results, outdoor duty group was showed higher than that of indoor duty group. During exercise stress test, heart rate, ventilation, and oxygen uptake, were checked by automatic gas analyzer. In results, outdoor duty group was showed higher than that of indoor duty group. In isokmetic knee joint muscle strength, peak torque and average power of indoor duty group was showed significantly lower than that of outdoor duty group. At Comparison of right and left knee joint, isokinetic strength, isokinetic power and isokimtic endurance of right knee joint was showed significantly higher then that of left knee joint, but in 60 degree per second, left knee joint was showed higher then that of right knee joint. Result from this investigation show greater personal training and fire quell a not, emergency rescue activity was increased knee joint muscle strength. Therefore, physical fitness and health maintenance programs for fire fighters have been developed, implemented and proven to reduce morbidity and mortality and financial expenditures.