Aspergillus sydowi sw 4-1이 생산한 지질이 흰쥐의 체내 지방 대사에 미치는 영향

고경,나혜복,박성오 서울여자대학교 자연과학연구소 1995 자연과학연구논문집 Vol.6 No.-

This study was conducted to investigate the effect of dietary lipid produced by Aspergillus sydowi sw 4-1 on the growth and lipid metabolism. Twenty weanling males Sprague-Dawley rats were fed the experimental diet. Aspergillus oil and soybean oil were supplied as a source of dietary fat respectively, at 5% and 10% by weight. There was no remarkable difference in food intake, body weight and weight of internal organs among experimental groups. The serum lipid and cholesterol content were of internal organs among experimental groups. The serum lipid and cholesterol content were significantly lower in the Asperillus oil group than in the soybean oil group, but there was no difference by the level of dietary lipid. The total lipid content in the liver and epididymal fat pad were higher in the Aspergillus oil group than that of the soybean oil group. The contents of both polar and non-polar lipid in the liver and the epididymal fat pad were affected by the diet. In the polar lipid of the epididymal fat pad, the Aspergillus oil group showed a higher result than the soybean oil group, and in relation to non-polar lipid, the Aspergillus oil group was lower than the soybean group. The composition of the major fatty acids of the epididymal fat pad was palmitic, oleic and linoleic acids. The Aspergillus oil group was lower in unsaturated fatty acid components than the soybean oil group. As a result Aspergillus oil had no visible toxic effect on the growth of rats, but there is a need to study further on the long term effect on Aspergillus oil as a animal feed.

배양조건이 Aspergillus sydowi SW4-1에 의하여 생산되는 지질 조성에 미치는 영향

고경,김성연,나혜복,박성오 서울여자대학교 자연과학연구소 1996 자연과학연구논문집 Vol.7 No.-

붉은 장미잎으로부터 분리 동정한 균주인 Aspergillus sydowi SW4-1은 균체생산 최적 배양기간인 17일간 보다 5일동안 더 배양한 경우에 nonpolar lipid 함량이 9.6% 증가하여 86.1%였고, 최적온도 보다 5℃ 높거나, 낮은 경우에 nonpolar lipid 함량이 더 증가하였으며 질소원은 NH₄NO₃를 사용한 경우 비극성 지질 함량이 가장 높아 80.9%였으며, urea의 경우 76.5%로 가장 낮았다. Aspergillus sydowi SW4-1의 비극성 지질의 주성분은 partial glyceride 8.5%, frree sterol 10.3%, triglyceride 72.4%였으며 대두유와는 조성에 많은 차이를 보였다. 비극성지방질의 주성분인 triglyceride는 배양기간이 경과함에 따라 12일에 74.6%에서 17일과 22일에 각각 72.4%와 67.7%로 감소하였다. 배양 온도가 높아짐에 따라 triglyceride함량은 21℃에서 73.1%였던 것이 33℃일 때 54.0%로 현저한 감소를 보였으며 반면에 free fatty acid는 21℃일 때 3.9%였던것이 33℃에서 12.4%로 약 3.5배나 증가하였다. Aspergillus sydowi SW4-1의 triglyceride함량은 질소원에 따라 72.4~73.2%의 분포를 보여 질소원에 따른 차이는 거의 없었다. 배양기간별 불포화 지방산의 합계는 12일에 60.4%, 17일에 68.0%, 22일에 59.4%로 최적일인 17일에 그 함량이 가장 높았으며 그 후에 감소하였다. 배양온도별 지방산조성은 최적온도인 27℃에서 불포화 지방산의 합계는 69%, 포화지방산의 합계는 31%로 21℃와 33℃의 66%와 33%보다 불포화 지방산 합계가 다소 높았으며 지방산 종류별로 보면 21℃와 33℃에 비해 27℃에서 palmitic acid함량은 낮고 straric acid와 linoleic acid의 함량은 높았다. 질소원에 따른 불포화 지방산의 합계는 69~71%로 큰 차이가 나타나지 않았으며 sodium nitrate를 질소원으로 사용한 경우 불포화 지방산의 합계가 가장 높았으며, 특히 linoleic acid함유율은 57.0%로 가장 높았다. A mold which produces a large amount of lipid was screened from the leaves of the red rose and was identified as Aspergillus sydowi SW4-1. Non-polar lipid content was recorded at 81.6% which was a 0.9% increase when incubated for 22 days, compared to the optimum incubation period identified as being 17 days. It was also shown that non-polar lipid content increased when incubated at higher, or lower temperatures by 5 degrees than the optimum temperature. Results of the examination with different nitrogen sources of CO(NH_2)_2, NH_4NO_3, and NaNO_3, represented the highest non-polar lipid content of 80.9%, when with NH_4NO_3,while they included the lowest content of non-polar lipid at 76.5% in case with Urea. The major constituents in the non-polar lipid were partial glyceride 8.5%, free sterol 10.3%, triglyceride 72.4%, which demonstrated substantial differences from those of soybean oil. Triglyceride as being a major component of non-polar lipid was observed to fall in content from 74.6% after 12 days of incubation to 72.4% after 17 days and to 67.7% after 22 days. Its content dropped as given higher temperatures from 73.1% at 21℃ to 54.0% at 33℃ . Free fatty acid, on the contrary, rose in its content by allmost 3.5 times from 3.9% at 21℃ to 12.4% at 33℃ . Triglyceride contents showed insignificant differeneces according to the various nitrogen sources, ranging from 72.4% to 73.2%, while the total contents of unsaturated fatty acid varied from 60.4% after 12 days, 68.0% after 17 days and 59.4% after 22 days, accordingly to the incubation period. Fatty acid was distinguished in the proportion of its cotents af different temperatures, resulting in the highest level of total unsaturated fatty acid of 69% at 27℃ , the optimum temperature, while showing 66% and 33% at 21℃ and 33℃, respectively. The content of palmitic acid was identified as being lower at 27℃ than at 21℃ or at 33℃, whereas the contents of stearic acid and linoleic acid were shown comparatively high. The total unsaturated fatty acid contents did not differ significantly by nitrogen sources, however, the contents of unsaturated fatty acid became high when sodium nitrate was introduced as the nitrogen source, of which content of linoleic acid was the highest 57.0%.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

Characteristics of Conversation in Frontotemporal Dementia: Comparison with Dementia of the Alzheimer’s Type

민준상(Jun Sang Min),윤지혜(Ji Hye Yoon),나덕렬(Duk L. Na),이윤경(Yoon kyoung Lee) 한국언어청각임상학회 2018 Communication Sciences and Disorders Vol.23 No.1

배경 및 목적: 전두측두치매(FTD) 환자는 전두엽의 전반적인 손상으로 인해 병의 초기부터 대화 시 화용적인 어려움을 보인다. 본 연구는 알츠하이머성 치매(DAT) 집단과의 비교를 통해 FTD 환자에서 두드러지는 대화 특성을 확인하였다. 방법: 총 50명(FTD 10명, DAT 20명, 정상[NA] 20명)을 대상으로 다양한 주제에 대하여 자유롭게 대화하도록 하였다. 자료 분석은 대화참여 지표와 대화화제 지표 및 대화방해 지표로 나누어 분석하였다. 결과: 첫째, 대화참여 측면에서 FTD 집단은 DAT, NA 집단보다 대화차례 주고받기 빈도와 대화차례당 발화 수 모두에서 유의하게 낮은 수행력을 보였다. 둘째, 대화화제 측면에서 FTD 집단은 DAT, NA 집단보다 화제 수, 화제당 대화차례 빈도, 화제유지비율, 화제전환비율은 유의하게 낮았고 화제이탈비율은 유의하게 높았다. 셋째, 대화방해 측면에서 FTD 집단은 DAT, NA 집단과 유의한 차이를 보이지 않았다. 논의 및 결론: 이러한 결과는 전두엽의 내측부위 손상으로 인한 동기부족으로 발화 수가 적고, 배외측 부위 손상으로 인한 전반적인 언어처리 및 작업기억능력의 결함으로 다양한 화제를 개시하거나 화제를 유지하는 것에 어려움을 보인 것으로 해석해볼 수 있다. Objectives: Due to the general damage to the frontal lobe, patients with frontotemporal dementia (FTD) could have pragmatic difficulties in conversation from early stages. The purpose of this study is to investigate the characteristics of conversation in patients with FTD through a comparison with dementia of the Alzheimer’s type (DAT), and then examine the relationship with general cognitive functions. Methods: Fifty subjects (FTD group = 10, DAT group = 20, normal adults [NA] group = 20) were engaged in free conversation on various topics. Results: First, regarding conversation participation, the FTD group showed significantly lower performance than the DAT group and the NA group in the frequency of conversation turn exchanges and the number of utterances during conversation turns. Second, regarding conversation topic, the FTD group showed a significantly lower number of topics, a lower frequency of conversation turns per topic, a lower rate of topic maintenance, and a lower rate of topic switching, but a significantly higher rate of topic breakaway than the DAT group and the NA group. Third, with regard to conversation disturbance, the FTD group did not show a significant difference in performance from the DAT group or the NA group. Conclusion: It is possible that FTD patients displayed a smaller number of utterances due to lack of motivation caused by the damage to the medial frontal lobe and have difficulties in topic maintenance and various topic initiation due to dysfunction of general language processing abilities and working-memory capacity caused by the damage to the dorsolateral area.

기관내관의 기낭압 조정이 수술 후 인후통에 미치는 영향

나혜경(Na Hye Kyoung),윤혜상(Yoon Haesang) 한국성인간호학회 2009 성인간호학회지 Vol.21 No.5

결혼 해체를 경험한 베이비부머 여성의 경제적 노후 준비 여부에 관한 연구

나지나 ( Na Gi Na ),백승애 ( Baek Seung Ae ),신은경 ( Shin Eun-kyoung ),김미혜 ( Kim Mee-hye ) 한국보건사회연구원 2010 保健社會硏究 Vol.30 No.2

본 연구는 노후준비를 통한 여성노인의 빈곤화를 예방하고자, 현재 결혼해체를 경험한 무배우자 베이비부머 여성의 노후준비의 현황과 이들의 노후준비 여부에 어떠한 변수들이 영향을 미치는지를 살펴보았다. 2007년 여성가족패널 1차년 자료를 이용하였으며 독립변인을 소인특성변수와 가용자원변수, 필요변수로 구분하였고 노후준비여부를 종속변수로 하여 로지스틱 회귀분석을 하였다. 주요 결과는 첫째, 결혼해체 유형 중이혼이 가장 높은 비율이었고, 다음으로 사별, 별거 순이었다. 학력은 고졸이 가장 많았으며 주거형태는 월세, 자가, 전세 순이었고 취업 한 경우가 66%를 차지하였다. 둘째, 결혼해체경험 여성들의 23.4%만이 경제적 노후 준비를 하고 있는 것으로 나타났다. 셋째, 교육수준과 월평균가구소득이 높을수록, 그리고 주택을 소유한 경우에 경제적 노후 준비를 할 가능성이 높았다. 노후경제상황에 대한 걱정은 경제적 노후 준비에 있어서 부정적인 영향을 미치는 것으로 나타났다. 이러한 결과를 바탕으로 정책적 제언을 제시하였다. The purpose of this study is to examine their determinants affecting the economic preparation for aging of Korea women in marital dissolution among baby boomers. Data of this study were drawn from the 2007 Korean Longitudinal Survey of Women and Families and the total number of subjects were 238 women widows or in divorce or separation. The analysis of data employed logistic regression analysis method. The conceptual framework for this study was based on Andersen`s behavioral model and Park, Chang Je`s model. Independent variables consists of predisposing characteristics, enabling resources, perceived need and dependent variable consist of preparation for aging. The results of analysis are summarized as follows. First, proportion of the women in marital dissolution among baby boomers with economic preparation for aging was low; 23%. Secondly, education level, monthly family income, housing ownership are positively and significantly associated with economic preparation for aging. Third, the concern of economic condition for aging is the negative factors on preparation for aging. However, the type and duration of marital dissolution did not give a significant effect on it.

항암화학요법으로 인한 말초신경병증 환자의 한방치험 1례

이지혜(Ji Hye Lee),박혜림(Hye Lim Park),이혜윤(Hye Yun Lee),조민경(Min Kyoung Cho),홍미나(Mi Na Hong),한창우(Chang Woo Han),최준용(Jun Yong Choi),박성하(Seong Ha Park),권정남(Jung Nam Kwon),이인(In Lee),홍진우(Jin Woo Hong),김소연(So Y 한의병리학회 2014 동의생리병리학회지 Vol.28 No.5

Transcriptional control of anthocyanin biosynthesis genes and transcription factors associated with flower coloration patterns in Gerbera hybrida

Naing, Aung Htay,Lee, Ji Hye,Park, Kyeung Il,Kim, Kyoung-ook,Chung, Mi Young,Kim, Chang Kil Springer-Verlag 2018 3 Biotech Vol.8 No.1

Improvement of Microbiological Safety of Sous Vide Processed Soybean Sprouts

Hye-Jung Kim,Na-Kyoung Lee,Dong-Sun Lee,Wansoo Hong,Sang-Rak Lee,Cheon-Jei Kim,Hyun-Dong Paik 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.1

Soybean sprouts which are a popular vegetable in Korea, are produced using the techniques of sous vide. The purpose of this study was to determine the effect of nisin and storage temperature on the microbiological and physicochemical qualities of sous vide processed soybean sprouts during storage in order to improve shelf-life and industrial applications. During storage of the cook-chilled soybean sprouts at 3℃, no development of mesophilic microorganisms was observed. However, at 10℃ storage without nisin, the number of mesophilic microorganisms increased markedly, whereas sprouts stored at the same temperature with nisin showed no observed increase. Psychrotrophic microorganisms, anaerobic microorganisms, and Bacillus cereus all showed similar trends. The ascorbic acid content, following the sequential heat processing of soybean sprouts through blanching and pasteurization decreased markedly during early storage, and stabilized thereafter. During storage, no major changes in the color or ascorbic acid content of samples at either temperature were observed. With regard to microbial and physicochemical qualities, the presences of nisin and storage temperature are important factors for extending shelf-life of soybean sprout.

Inhibition of Listeria monocytogenes in Vacuum or Modified Atmosphere - Packed Ground Beef by Lactococcal Bacteriocins

Hye Jung Park,Na-Kyoung Lee,Kee-Tae Kim,Jung-Uk Ha,Dong Sun Lee,Hyun-Dong Paik 한국식품영양과학회 2003 Preventive Nutrition and Food Science Vol.8 No.2

We investigated the antagonistic effects of two lactococcal bacteriocins, nisin or lacticin NK24, on the growth, the survival of Listeria monocytogenes in vacuum or modified atmosphere-packaged ground beef. Ground beef was inoculated with approximately 3 log colony-forming units (CFU) of L. monocytogenes ATCC 15313 culture per gram of ground beef. Inoculated samples were blended with/without 100 AU/g nisin or lacticin NK24,, subsequently vacuum or modified atmosphere packed at 4℃. Listeria in the bacteriocin-treated, control samples was subsequently isolated from both vacuum, modified atmosphere packs, enumerated as CFU on Listeria Isolation Agar medium. Microbial counts in ground beef treated with bacteriocin declined steadily, while those of non-treated beef samples increased steadily. The results obtained demonstrate that nisin inhibits the growth of L. monocytogenes more effectively than lacticin NK24 at 100 AU/g. The use of lactococcal bacteriocins, such as nisin or lacticin NK24, in vacuum or modified atmosphere packaging offers a promising approach for eliminating or reducing the risk of L. monocytogenes contamination in ground beef.