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In order to study the morphological changes of Corti’s organ in the rat treated with cisplatin and tobramycin, thirty-three rats, weighing between 200∼250g, were used, and divided into 4 groups, i.e., 3 rats were used for control, and 30 rats were used for 3 experimental groups. Each experimental group was divided into cisplatin-treated, tobramycin-treated, and both cisplatin and tobramycin treated group with 10 rats. Rats were injected with a single dose of 7.7㎎/㎏ of cisplatin intraperitoneally and 50㎎/㎏/day of tobramycin for 2 days intramuscularly, respectively or both. Treated rats were sacrificed on the 3rd and 5th day after the first injection. Results were as follows; 1. Cisplatin-treated group: Outer hair cells of Corti’s organ were degenerated with the formation of vacuoles and appearance of lysosomes, however, inner hair cells showed mild vacuolization. 2. Tobramycin-treated group: NO pathological changes were found in Corti’s organ. 3. Combined cisplatin and tobramycin treated group: Outer and inner hair cells showed vacuolization, appearance of lysosomes, myelin figures and multivesicular body. There were also multivesicular body in the inner phalangeal cell and myelin figure in the Reissner’s membrance. 4. Degeneration of Corti’s organ in the combined treated group was more sever than those of the single cisplatin-treated or tobramycin-treated group. 5. NO pathological differences of Corti’s organ in duration of postinjection were manifested in any groups. It can be concluded that cisplatin induces toxicity to inner ear and cisplatin enhance toxicity more in continued treated with tobramycin.
Purpose : The Mirizzi syndrome is relatively rare and preoperative diagnosis of this disease is difficult. In 1978, Morelli suggested the subclassification of the Mirizzi syndrome into acute or chronic form. We experienced 5 cases of acute form. We analysed clinical features, preoperative radiologic findings and operative findings of 18 cases including acute forms which were diagnosed as Mirizzi syndrome and should suggest the modified classification of Mirizzi syndrome for choice of appropriate treatment. Method : From January 1995 to December 1998, 18 cases, of which 8 cases were diagnosed at Soonchunhyang University Chunan Hospital, and 10 cases were reported in the Korean Journal were retrospectively analysed with regard to clinical features, preoperative radiologic findings and operative findings. According to the clinical features, whole cases were divided into type Ⅰ(acute form) and type Ⅱ(chronic form) and then each type of cases were subclassified according to preoperative radiologic findings and operative findings. Results : Of 18 cases there were 5 cases in type Ⅰ(27.8%), 13 cases in type Ⅱ(72.2%). Type Ⅱb was most common. Type Ⅰa cases were treated only with cholecystectomy. We applied cholecystectomy, T-tube choledochostomy and patch technique in type Ⅰb and thpe Ⅰc cases. Cholectystectomies including removal of gallstones and internal drainage procedures were done in type Ⅱ chronic forms. Conclusion : The acute form(Type Ⅰ) of Mirizzi syndrome was suggested by Morelli might be subclassified into typeⅠa,Ⅰb and Ⅰc following the presence of the necrotic defect in common hepatic duct. Through the modified classification of Mirizzi syndrome based on clinical feature, preoperative radiologic findings and operative findings, we can choice appropriate treatment.
<P>The delayed hydride cracking (DHC) tests were conducted on Zr-2.5Nb compact tension specimens with the test temperatures reached by a heating and a cooling. The Zr-2.5Nb specimens were either furnace-cooled or water-quenched after a hydrogen charging treatment to contain 10 to 100 ppm H. On an approach to the test temperatures by a cooling, both the Zr-2.5Nb specimens showed the DHC velocity increasing with an increasing temperature over a temperature range of 100–300°C, irrespective of the cooling rate. However, on an approach to the test temperatures by heating, the furnace-cooled Zr-2.5Nb showed a DHC arrest at temperatures over 180°C and no DHC at 250°C, and the water-quenched ones did have a DHC growth, even at 250°C. Using Kim’s DHC model we elucidate the DHC arrest in the furnace-cooled Zr-2.5Nb at temperatures over 180°C and the DHC growth in the water-quenched specimen, even at 250°C, upon an approach by a heating.</P>
An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal trasmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compard the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residuse in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.
Neuropeptide Y(NPY) was first isolated from porcine brain. This discovery has lead some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study the distribution pattern of neuropeptide Y-immunoreactive neurons was examined in the hippocampal complex of Striped field mouse in Korea(Apodemus agrarius coreae) Experimental animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through the left ventricle and the ascending aorta. The brains were removed and transferred 10%, 20% and 30% sucrose solution. Sections were cut on a cryostat into 40㎛-thick. The tissue was immunostained with free-floating and avidin-biotm complex(ABC) method. The results obtained in this study were summarized as followings. 1. The most of NPY-IR neurons in hippocampus proper were found in the polymorphic layer and a few was found in the pyramidal layer. 2. The process run vertically in the deep polymorphic layer and run parallel to corpus callosum in superficial polymorphic layer. 3. The shapes of neurons in hippocampus proper were varied of polymorphic, oval or round, and the nerve fibers were found in the molecular laver and the hippocampal fissure. 4. The highest density in number of NPY-IR neurons on dentate gyrus were found in hilar area of dentate gyrus. 5. The most of NPY-IR nerve fibers were found in molecular cell layer.
In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.
The purpose of this study is to provide the information whether Korean dance improves the physical fitness and which factors should be reinforced and improved too get a better performance on the assumption that performance has something to do with physical fitness. The subjects were 25 females who were majoring in Korean dance in W University. They were divided into 3 groups according to the level of performance. Each group took a test which measured 6 physical fitness factors so as to compare each group's physical fitness. The results of analysis were as follows : 1. The flexibility (P<0.1) was the only factor that affect the performance of Korean dance. The other factors came power, muscular endurance, agility, strength, and balance in order. Ragression Equation for correlation between performance score and flexibility is : Performance score = 64.4 + 0.0181 (forward trunk flexion)^2. 2. In the correlation between performance score and physical fitness factors, only flexibility showed a little relation(r=0.33). 3. The flexibility showed significant difference in comparison with physical fitness between groups according to the level of performance (P<0.1). In conclusion, it was flexibility among physical fitness factors that affect the performance of Korean dance.
To evaluate the morphological and functional changes of the hepatic cells of hybrid chick according to its growing stages, the volume of cytoplasm, nucleus and nucleolus were measured in 10 specimens of each group; the group of 2nd day, 14th day, 2nd month, 5th month and the group of 11th month after hatching. The results were as follows; 1. The volume of nucleus and nucleolus was increased from the 2nd day to the 14th day after hatching (actively growing period) and from the 5th month to the 11th month after hatching (the period of hypertrophy). The volume chage of cytoplasm appears oppositely to that of nucleus and nucleolus. 2. The nucleoplasmic index was increased at actively growing period and the period of hypertrophy, and the ratio of nucleolar volume to nuclear volume was increased at actively growing period, but unchanged at the period of hypertrophy.
Now a day, the more Proportion of concrete used in practice is exposed to serious chemical attack Chemical reactions that involve formation of expansive products in hardened concrete can lead to certain harmful effects. In this study, it is to compare and investigate the chemical resistance of natural and recycled aggregate concrete by attacked Na₂SO₄according to W/C ratio. The result of this study is that the chemical resistance of recycled aggregate concrete is lower than natural aggregate concrete. But it is developed the chemical resistance by decreasing the W/C ratio.