인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

전문계 고등학교 교사들의 이러닝 활용과 영향요인 간의 구조방정식 모형

박성열,남민우,박운구 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.2

이 연구는 전문계 고등학교 교사들을 대상으로 이러닝을 활용하는데 영향을 미치는 요인들을 파악하고, 이들 요인들간의 인과관계성을 구조 모형적인 측면에서 분석 · 설명하는 데 중점을 두고 있다. 연구모형에 포함된 요인은 교직경력, 컴퓨터 사용경험, 이러닝 지원부문, 이러닝의 사회/조직 관련성 등의 외생변인과 이러닝 활용을 위한 컴퓨터 리터러시, 이러닝 활용 의향도 등의 내생변인 등이며, 요인들간의 상관계수를 산출하여 그 행렬을 이용, LISREL 프로그램에 의해 공변량 구조 이론 모델 분석을 수행하였다. 이 연구에서 제시된 모델 분석을 통한 연구결과는 다음과 같다. 첫째, 교사들의 이러닝 활용을 위한 컴퓨터 리터러시에는 설정된 외생변인 중 컴퓨터 사용경험의 영향을 가장 크게 받고 있으며, 다음으로 이러닝 지원정도, 사회/조직 관련성 등의 순으로 영향을 받고 있다. 둘째, 최종 내생변인인 교사들의 이러닝 활용 의향도에는 외생변인 중 이러닝의 사회/조직 관련성만이 영향을 미치는 것으로 분석되었다. 셋째, 내생변인간의 관계성을 분석한 결과 교사들의 이러닝의 활용 의향도는 이러닝 활용을 위한 컴퓨터 리터러시에 매우 큰 영향을 받고 있음을 보여 주었다. This study was designed and implemented to identify factors affecting e-learning use of vocational high school teachers based on structural equation model. The model career, computer experience, e-learning support, social and organizational norm related to e-learning as exogenous variables and computer literacy and intention to use as endogenous variables. The researcher produces a correlation matrix among the variables and run a structural model using LISREL program. The major findings after are as follows. First, teachers' computer experience was the most significant factors affecting their computer literacy to utilize e-learning, followed by e-learning support and soial and organizational norm. Second, intention to use e-learning, terminal exogenous variable was influenced by e-learning social and orginizational norm among exogenous variables. Third, in terms of the relationships between endogenous variables, intention to use e-learning was largely influenced by computer literacy from the model analysis.

논토양중 카드뮴 유효도와 수도의 흡수이행에 미치는 석회 및 Humic acid 사용효과

김민경,김원일,정구복,박광래,윤순강,엄기철 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

토양중 중금속이 식물에 흡수 및 이행되는 것을 경감시켜 여기에서 생산된 농산물의 안전성 향상을 위한 기초자료를 확보하고 논토양의 카드뮴 유효도와 흡수이행에 미치는 석회와 humic acid 처리 효과를 구명하기 위하여 실내경험과 포트실험을 수행하였다. 실내실험에서 공시토양에 2.5와 5.0 ton/ha의 석회와 1%와 2% humic acid를 처리한 후 토양중 가용성 카드뮴 함량 변화를 조사한 결과, 두 처리 모두 14일까지는 담수상태에서 처리효과로 인하여 가용성 카드뮴 함량이 감소하다가 그 이후에는 토양의 완충능에 의해 다시 증가하는 경향이었다. 이때, 토양의 가용성 카드뮴 함량은 토양 pH 및 양이온치환용량과 부의 상관이 있었다. 2.5 ton/ha의 석회와 1% 의 humic acid를 처리한 후 담수시켜 안정화된 다음 벼를 재배하여 조사한 결과, 석회와 humic acid 처리에 비해 분얼기와 수확기의 치화태 및 수용태 카드뮴 함량이 낮아졌으나, 분얼기에 비해 수확기에 토양중 이동이 어려운 산화물 및 탄산염태와 황화물 및 잔류태의 함량이 증가하였다. 식물에 흡수가 용이한 형태로 알려진 치환태와 수용태는 모두 토양 pH와 고도의 부의 상관이 있었다. 수확기의 줄기, 잎 및 현미 건물중은 석회 및 humic acide 처리구에서 대조구에 비해 모두 높았고, 특히 석회 처리구의 건물중이 현저하게 높았다. 석회 처리구에서 줄기와 잎의 카드뮴 함량은 각각 1.01과 0.37㎎/㎏으로 대조구와 비슷하였으나, 뿌리나 현미의 카드뮴 ?량은 각각 2.11과 0.09㎎/㎏으로 대조구에 비해 낮았다. 지상부와 뿌리의 카드뮴 함량은 humic acid 처리에 의해 현저히 낮아졌으며, 특히 현미로의 카드뮴 이행도 월등히 낮게 나타난다. This study was conducted to know the effect of lime and humic acid on cadmium availability and its uptake by plant grown in contaminated paddy soils with heavy metal. The treatment levels of lime were 2.5 and 5.0 ton/ha and that of humic acid were 1 and 2%. The contents of 0.1 N HCl extractable Cd were reduced with lime and humic acid and were negatively correlated with CEC as well as soil pH. The sequential extraction procedure was used to fractionate the heavy metals in soil into the designated from exchangeable (0.5 M KNO₃), water soluble (H₂O), organically bound (0.5 M NaOH), carbonate (0.05 M Na₂-EDTA) and sulfide/residual (4 M HNO₃). In soil amended with 2.5 ton/ha lime and 1% humic acid, chemical forms of Cd at tillering stage were predominant exchangeable+water soluble extractable Cd, whereas that at harvesting stage were predominant carbonate+sulfide/residual extractable Cd. The exchangeable forms of Cd in soil with lime and humic acid were negatively correlated with soil pH during the harvesting period. Total absorbed Cd of paddy rice tended to occur in the order of root > stem > leaf > brown rice. Cd con- tents of brown rice with lime and humic acid treatment were 0.09 and 0.08 mg/kg, respectively. That were lower than control, 0.20 ㎎/㎏. It could be that treatment of lime and humic acid in polluted soil by heavy metals would reduce the uptake of heavy metals by plants and be a temporary method of reclamation at the highly heavy metal contaminated soils.

좌심실 이완기 장애 평가에서 Atrioventricular Plane Displacement의 임상적 중요성

정연손,조구영,이태유,김근숙,최창순,류제헌,최민호,박우정,임종윤,이영 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.12

Facile Synthesis and Radioiodine Labeling of Hypericin

Kim, Sang-Wook,Park, Jeong-Hoon,Yang, Seung-Dae,Hur, Min-Goo,Kim, Yu-Seok,Chai, Jong-Seo,Kim, Young-Soon,Yu, Kook-Hyun Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8

Hypericin (1,3,4,6,8,13-hexahydroxy-10,11-dimethylphenanthro[1,10,9,8-opqra]perylene-7,14-dione), an antidepressant which is also known to be a potent protein kinase C (PKC) inhibitor was synthesized as a precursor for radioiodine labeling via two step reactions. Malignant glioma cells express higher PKC activity compared to untransformed glial cell. Here we report the synthesis and radioiodine labeling of hypericin as a potential brain tumor imaging radiopharmaceutical. The reference compound, 2-iodohypericin, and its radiolabelled analogues, 2-[$^{123}I$]iodohypericin and 2-[$^{124}I$]iodohypericin have been prepared by the reaction of hypericin with NaI or [$^{123}I$]NaI or [$^{124}I$]NaI. The labeling yield was 60-65% for each analogue and the optimal reaction time was 10 min. The purification and isolation of the labelled products were achieved by a reversed-phase HPLC.

폐암과 폐 염증성질환의 동적양전자방출단층검사(Dynamic 18F-FDG PET)의 유용성

박훈희(Hoon-Hee Park),노동욱(Dong-Wook Roh),김세영(Sei-Young Kim),동경래(Dong-Kyeong Rae),이민혜(Min-Hye Lee),강천구(Chun-Goo Kang),임한상(Han-Sang Lim),오기백(Ki-Back Oh),김재삼(Jae-Sam Kim),이창호(Chang-Ho Lee) 대한방사선과학회(구 대한방사선기술학회) 2006 방사선기술과학 Vol.29 No.4

  목적: 양전자방출단층검사(Fluorine-18 2-Deoxy-D-Glucose positron emission tomography: ¹?F-FDG PET)는 가시적 영상분석과 반정량적 섭취계수 분석 방법을 사용하여 폐의 염증성 질환과 폐암을 구별 및 진단하는 데 유용하다. 일반적으로 표준화섭취계수(Standardized Uptake Value: SUV)가 폐 질환의 진단에 사용되지만, 이는 폐 조직에 있어서 반정량적, 정량적 분석을 통한 동적인 정보를 포함하지 않는다. 그러므로, 본 연구는 ¹?F-FDG PET 검사에서 폐 질환의 동적 섭취 분석이 보다 정확한 대상질환의 진단을 유용하게 할 수 있을 것이라는 가정하에 시간-방사능 곡선(Time-Activity Curve: TAC), 표준화섭취계수-동적곡선(Standardized Uptake Value-Dynamic Curve: SUV-DC), 패트락 분석법(Patlak analysis: Glucose Metabolic Rate(MRGlu))을 토대로 얻은 분석방법을 이용하여 진단적 유용성을 평가하였다.<BR>  대상 및 방법: 폐 질환을 가진 17명의 환자를 대상을 하여, 각각 60분간 Dynamic ¹?F-FDG PET검사를 시행하였다. 획득한 정보를 통하여 폐질환의 형태를 따라 관심영역(Region of Interest: ROI)를 그린 후, 반정량적 분석인 TAC, SUV-DC와 정량적 분석인 Patlak analysis를 각각의 군에 따라 분석하여 비교 분석하였다.<BR>  결과: Dynamic ¹?F-FDG PET을 통한 분석결과, TAC 형태는 초기 혈류상에서 폐의 염증성 질환과 폐암의 구분이 어려웠으나, 시간이 지날수록 폐암의 곡선이 염증성 질환의 경우보다 확연히 증가하였다. SUV-DC의 경우는 TAC 형태와 거의 유사한 형태를 가졌다. Patlak analysis 분석결과, 대동맥 영역에서는 폐의 염증성 질환이 폐암보다 높은 혈액 방사능을 보였으나 시간이 지남에 따라 염증성 질환의 혈액 방사능이 극히 낮아졌다. 반면, 병변 조직에서는 폐암이 가장 높은 섭취를 보였으며 폐의 염증성 질환은 중간 정도에 머물렀다.<BR>  결론: TAC와 SUV-DC 분석에서 폐암과 폐의 염증성 질환은 각각 고유한 그래프 형태를 띠었으며 Patlak analysis에서 역시 주목할 만한 차이를 보였다. 따라서 이러한 분석법을 토대로 좀더 깊이 있는 연구가 진행된다면 비침습적으로 폐의 질환을 구별하는 데 보다 적절하고 유용한 진단적 정보를 얻을 수 있을 것이다.   Purpose: The diagnostic utility of fluorine-18 2-deoxy-D-glucose positron emission tomograhpy (¹?F- FDG PET) for the non-invasive differentiation of focal lung lesions originated from cancer or inflammation disease by combined visual image interpretation and semi-quantitative uptake value analysis has been documented. In general, Standardized Uptake Value(SUV) is used to diagnose lung disease. But SUV does not contain dynamic information of lung tissue for the glucose. Therefore, this study was undertaken to hypothesis that analysis of dynamic kinetics of focal lung lesions base on ¹?F-FDG PET may more accurately determine the lung disease. So we compared Time Activity Curve(TAC), Standardized Uptake Value-Dynamic Curve(SUV-DC) graph pattern with Glucose Metabolic Rate(MRGlu) from Patlak analysis.<BR>  Methods: With lung disease, 17 patients were examined. They were injected with ¹?F-FDG over 30-s into peripheral vein while acquisition of the serial transaxial tomographic images were started. For acquisition protocol, we used twelve 10-s, four 30-s, sixteen 60-s, five 300-s and one 900-s frame for 60 mins. Its images were analyzed by visual interpretation TAC, SUV-DC and a kinetic analysis(Patlak analysis). The latter was based on region of interest(ROIs) which were drawn with the lung disease shape. Each optimized patterns were compared with itself.<BR>  Results: In TAC patterns, it hard to observe cancer type with inflammation disease in early pool blood area but over the time cancer type slope more remarkably increased than inflammation disease.<BR>  SUV-DC was similar to TAC pattern. In the result of Patlak analysis, In time activity curve of aorta, even though inflammation disease showed higher blood activity than cancer, at first as time went by, blood activity of inflammation disease became the lowest. However, in time activity curve of tissue, cancer had the highest uptake and inflammation disease was in the middle.<BR>  Conclusion: Through the examination, TAC and SUV-DC could approached the results that lung cancer type and inflammation disease type has it’s own difference shape patterns. Also, it has outstanding differentiation between cancer type and inflammation in Patlak and MRGlu analysis. Through these analysis methods, it will helpful to separation lung disease.

PLCM을 이용한 카오스 블록 암호화 기법

이성우,이민구,박정렬,신재호 東國大學敎 産業技術硏究院 2005 산업기술논문집 Vol.15 No.1

본 논문에서는 동력학적 특성이 좋은 PLCM을 이용한 256비트 길이의 암호 키와 32비트 데이터 블록의 카오스 블록 암호화 기법을 제안한다. 본 논문에서 제안한 기법은 256비트의 키에서 각 암호화 단계마다 사용될 32비트의 세션 키 두 개를 생성해서 PLCM의 초기 값과 반복회수를 결정하는데 사용한다. 본 제안 기법은 피드백 구조와 대치 기능의 S함수를 통해 암호 강도를 향상시켰다. 또한 실험 결과와 안전성 분석을 통해 본 제안 기법이 통계적(Statistical) 공격에 매우 강함을 보여준다. In this paper, we propose a chaotic block encryption scheme using a PLCM (Piecewise Linear Chaotic Map) having a good dynamical property. The proposed scheme has a block size of 32 bits and a key size of 256 bits. In the proposed scheme we use two 32-bit session keys generated from 256-bit key to decide the initial value and the number of iteration of PLCM. To enhance the strength of security we use a feedback structure and a S (Substitution) function. With results of test and analyses of security we show the proposed scheme is very secure against statistical attacks.

Spontaneous Additive Nanopatterning from Solution Route Using Selective Wetting

Jeong, Hyeonho,Moon, Hanul,Kim, Han-Jung,Yoon, Min,Park, Chang-Goo,Oh, Yong Suk,Sung, Hyung Jin,Choi, Dae-Geun,Yoo, Seunghyup American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.31

<P>Nanopatterns of functional materials have successfully led innovations in a wide range of fields, but further exploration of their full potential has often been limited because of complex and cost-inefficient patterning processes. We here propose an additive nanopatterning process of functional materials from solution route using selective wetting phenomenon. The proposed process can produce nanopatterns as narrow as 150 nm with high yield over large area at ultrahigh process speed, that is, the speed of solution dragging, of up to ca. 4.6 m·min<SUP>-1</SUP>. The process is highly versatile that it can utilize a wide range of solution materials, control vertical structures including pattern thickness and multistacks, and produce nanopatterns on various substrates with emerging form factors such as foldability and disposability. The solution patterning in nanoscale by selective wetting is enabled by corresponding surface energy patterns in high contrast that are achieved by one-step imprinting onto hydrophobic/hydrophilic bilayers. The mechanisms and control parameters for the solution patterning are revealed by fluid-dynamic simulation. With the aforementioned advantages, we demonstrate 25 400 pixel-per-inch light-emitting pixel arrays and a plasmonic color filter of 10 cm × 10 cm area on a plastic substrate as potential applications.</P> [FIG OMISSION]</BR>

Status of research on quarantine treatment and application of sulfuryl fluoride to wood destorying pests

Min-Goo Park 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10

Research on alternative fumigants to replace methyl bromide (MB), a harmful ozone-depleting substance and highly toxic pesticide, began in 2007. Initial tests focused on using ethyl formate for fruits and EDN for wood. Subsequently, alternative techniques were developed for a total of 94 plant types using ethyl formate, EDN, and phosphine, demonstrating Korea's superior performance compared to other countries, especially in practical applications. However, when it comes to wood products, the primary users of methyl bromide, EDN is scarcely used due to concerns about worker safety. An emerging alternative to MB is sulfuryl fluoride (SF), widely employed in North America to control wood-damaging pests like termites. SF's advantages include a lower boiling point and higher vapor pressure, making it effective against wood pests. Yet, certain bark beetle eggs require higher doses and longer treatment periods, resulting in established international standards (e.g., 120g/m3 for 48 hours at 20℃), posing challenges for field applications. Recently, the APQA initiated basic research to apply SF to wood. They are assessing SF's effectiveness against various wood-destroying pests, including termites, bark beetles, and long horn beetles, while also evaluating the feasibility of mixed treatments to reduce dosage. Once SF fumigation standards are established, it is expected to significantly reduce methyl bromide use in commonly used wood types, contributing to ozone layer protection and enhancing fumigation work safety.

The risk of Methyl bromide fumigation work on the quarantine area

Min-Goo Park,Jungmi Choi 한국응용곤충학회 2022 한국응용곤충학회 학술대회논문집 Vol.2022 No.10