저장 온도 및 상대습도가 비타민 정제 중 지용성 비타민 함량의 변화에 미치는 영향

김동섭,이재황,김세곤,이동언,박석준,이진희,이강표,최성원,백무열 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

김치발효중 가스발생에 따른 김치의 품질 특성 변화

이재황,김동섭,백무열 경희대학교 생명자원과학연구원 2004 硏究論文集 Vol.23 No.-

김치의 발효과정 중의 품질의 변화에 대하여 측정한 결과 온도가 5℃에서 20℃로 높아질수록 숙성속도가 빠르고 발생한 gas의 양도 많았지만, 일정기간 통안 발효가 진행된 후에는 gas의발생량이 줄어들었다. pH와 환원당량은 gas가 발생하는 특성과 유사하게 발효초기에만 급격히 떨어지고 발효가 진행될수록 변화가 적어지는 특성을 보였으나, 온도에 따른 최종측정치의 변화는 보이지 않아 gas가 발생하는 특성과의 차이를 보였다. 산도의 증가 형태 역시 gas의 증가 형태와 매우 유사하게 온도가 높아질수록 숙성속도가 빨라졌지만 최종 산도의 값은 각 온도별로 큰 차이를 보이지 않았다. 총균수는 gas 발생량의 변화와 비례관계는 보이지 않았지만 gas의 생성속도가 느려졌을 때 그 수가 줄어들었고 숙성 중 젖산균수의 변화는 총균수의 변화와 매우 유사한 경향을 보였다. 초기 젖산균수가 급상승하여 비슷한 수준을 유지할 때 gas는 초기젖산균수에 맞춰 증가하다가 젖산균수가 비슷한 시기일 메에도 젖산균의 증식이 계속되어 비교적 gas의 증가가 큰 폭으로 증가하였다. Effect of gas on quality of Kimchi was investigated at different fermentation temperatures. During Kimchi fermentation, the amount of gas, total numbers of viable cells, numbers of lactic acid bacteria and acidity increased but pH and reducing sugars decreased in all temperatures. The amount of gas increased with increasing fermentation temperature but the final volume of gas was not significantly different. Acidity, reducing sugars and pH showed similar patterns with gas production. The amount of gas was not depend on total numbers of viable cells but highly influenced by the numbers of lactic acid bacteria. This study provides the fundamental information on the relationship between fermentation temperature and shelf-stability of Kimchi.

韓國産 자작나무科의 花粉分類學的 硏究

李相泰,金茂烈 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

The pollen grains of the Korean Betulaceae including 15 species and 4 varieties of 5 genera, were examined to describe the taxonomic characters and to find the phylogenetic position of the family and the inter- and intrageneric as well as the inter- and intraspecific relationships. The possession of 3-6-pororate apertures suggested that the Betulaceae is advanced and has been derived rather from the ancestor of the tricolporate Fagaceae than from the ancestor of the tricolpate Hammamelidaceae. Pollen morphology supported the familial division into Section Betuleae and Coryleae, i. e. vestibula are prominently developed in the former while not in the latter In Section Betuleae, because of the characteristic arcus as well as the high number of pores. Alnus was regarded to be more advanced than Betula. In Section Coryleae, Corylus with inconspicuously developed vestibulum was thought to be the most primative in the family as well. The Carpinus pollen with a higher pore number and larger pollen size suggested to be more advanced than the Ostrya pollen. In Alnus, the mean pore number of the hirsuta group (4.28) was significantly higher than that of the japonica group (4.80). In betula, Series Albae had smaller pollen grains (26.03×20.68㎛) than the Costatae (34.60×28.67㎛). In Corylus, the heterophylla group and the sieboldiana group showed the almost identical pollen morphology. In Carpinus, the tschonoskii group possessed 4 of 5 pores, whereas the cordata 3 pores. The Ostrya pollen exhibited a good similarity with that of C. cordata.

위 유문동 평활근세포의 Caffeine 활성화 전류에 미치는 Ascorbate의 효과

이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.4

In order to observe the effects of ascorbate on the background current in pyloric circular smooth muscle cells, caffeine of which action was fairly clarified, was used to induce the current systems. To block all of the known currents, various blocking agents were added in the external bathing solution. A part of pyloric circular muscle tissue was excised out, and enzymatic isolation technique was used to obtain Ca^2+ -tolerant single circular muscle cells. The whole cell mode of patch-clamp experiment was conducted. The results were as follows : 1. Ascorbate induced the increase of inward currents in the hyperpolarized potential ranges, and quite a large increase of outward currents in the depolarized ranges. 2. Caffeine also induced similar results, but the magnitude of outward current was smaller than that of assorbate in the depolarized ranges. 3. These phenomena were disappeared by addition of the anion channel blocker. 4. Replacement of external NaCl with NMG·Cl was rarely affected the effects of ascorbate and and caffeine. But the effects of ascorbate and caffeine were almost abolished when sxternal Cl^- was substituted for aspartate. These results suggested that ascorbate affected chloride current which thought as a component of the background currents in the pyloric circular smooth muscle cells.

피지선 모반에서 발생한 유두상 한선종 1예

성열오,서무규,문형도,박기흠,이창우,이경섭 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

Syringoadenoma papilliferum occurs as a solitary lesion appearing usually on the scalp. It's origin is not definitely known, and about one-third of cases are associted with nevus sebaseous. We describe a case of syringoadenoma papillferum associated with nevus sebaseous in a 29 year-old female who had developed bean sized verrucotic nodules on the Rt. temporal area of the scalp since 6 years after removal of nevus sebaceous. Histopathologic findings showed hyperkeratosis, acanthosis and villi that projected into a cystic lumen. And the villi were surrounded by two cell layers.

산업근로자의 식생활 의식행태 조사

김무룡,황연자,차경미,이순자,위광복,남철현,김기열,김홍길 慶山大學校 保健福祉硏究所 1997 保健福祉硏究 Vol.1 No.-

This study was carried out to grasp the awareness and habits of food intake and use the information for health education program in order to help healthy dietary life. The survey was done in cooperation with health manager in industries on 1,200 workers who was selected randomly from 5 types of industries from Sep. 2 to Oct. 31, 1995. The results are summarized as follows: 1) The percentage of subjects who assess themselves "Healthy" was more higher in female, in 20∼29 age group, in single, in daytime workers, in college graduate than other groups respectively. 2) As for the degree of health education satisfaction, 35.5% of the subjects answered "dissatisfaction" 3) As for the exercise, 52.7% exercised for their health and the rate of exercise was more higher in male, in 30 years age group, in single than other groups respectively. 4) The rate of smoking was 57.6% and the rate of drinking alcohol was 47.3%. 5) 92.8% of the subjects got stress everyday and the rate is more higher in female, in less than 19 years old, in single, in longer working duration, in lower income, in high school graduates, in workers of product field line than other groups respectively. 6) As for drinking times of coffee or soft drink, two times per day was 29.5%, more three times per day was 28.0%. As for the eating speed of meal, 46.0% of the subjects answered "fast" and the rate was more higher in male, in 30 years age group, in married group, than other groups respectively. 7) With regard to the level of preference to instant food, 23.1% preferred to take instant food. As for the level of preference to hot and salty food, 28.1% preferred to have hot and salty food. 8) The score of eating behavior was 3.38±32 point to 6 point full marks. 9) With regard to the correlation among related variables, the variables of gender, marital status and working duration had positive relation with the variables of regular meal intake, the preference to food intake between regular meals.

위 평활근 세포의 활동전압을 이루는 CI^-전류성분에 관한 연구

방효원,이종찬,이무열,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1993 中央醫大誌 Vol.18 No.3

Chloride current has been known as a part of backgroud current. Recently, its roles in the maintenance of action potential have much attention. The purposes of the present study are clarifying the chloride current component which might play an important role in generation and maintenance of the action potential in the pyloric circular smooth musele cells. We used the whold cell voltage clamp techniques to assess the current and voltage responses induced by caffeine superfusion. The results were as follow: 1. Caffeine superfusion induced the increase of inward currents in the hyperpolarized potential ranges, and the increase of outward currents in the depolarized ranges. 2. Sodium ion removal from the external medium and the addition of cadmium ion rarely affected these current response. 3. Duration of the action potential was elongated by application of the caffeine and modified by the change of the intracellular chloride ion concentraion. 4. The effect of caffeine superfusion on the action potential disappeared by the addition of the anion channel blocker. The results suggest that caffeine superfusion may prolong the duration of repolarized phase of the action potential probably by the chloride current activation in the gastric antral smooth muscle.

Ca^(2+)―작동성 Cl^-전류에 미치는 이차 전령들의 효과

송인섭,이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1993 中央醫大誌 Vol.18 No.3

The anion movements across the cell membrane were regarded as apart of passive distribution according to the cation movements. Recently, from the developement of patch clamp technique new concept for anion channels were introduced. And their roles in the genesis of action potential and resting potential were noticed. So we clarified the existence of the chloride channel in the smooth muscle cells and studied its nature in regard to the roles of the second messangers. We used the whold cell voltage clamp technique to study the current responses induced by carbachol application in the circular smooth muscle cells of the gastric antrum of the rabbit. The results were as follows; 1. Application of the carbachol induced the increase of inward currents in the hyperpolarized potential ranges, and the increase of outward currents in the depolarized ranges. 2. Even in the cases of blocking all the known currents, these current components were observed. 3. Sodium ion removel from the external medium rarely affected these current responses, and the addition of cadmium ion did not make any remarkable change. 4. Known second messangers such as c-AMP, c-GMP, heparin, A1F_4 rarely affected these current components. 5. Lowering of intracellular calcium ion concentration drastically reduced these current responses. Form the above results we can suggest that the carbachol induced current responses contained chloride currents which were activated by only intracellular calcium ion, not by any other second messegers in the gastric antral smooth muscle cells.

배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

위 유문동 평활근 세포에서 Caffeine에 의해 활성화되는 이온 전류에 관한 연구

정인원,이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.3

In order to clarify the existence of the non-selective cationic channel of CI^- -channel in gastric antral smooth muscle cells which suggested to play a critical role in generation and maintenance of depolarization and exitability, the whole cell mode of patch-clamp experiment was conducted. Following results were obtained ; 1. Puff injection of caffeine induced the increase of inward currents in the hyperpolarized holding potential ranges, and the increase of outward currents in the hypopolarized range. 2. In the case of blocking of all known currents, these current components were observed. 3. These current components were disappeared when known as a Cl^- -channel blocking agent was treated and intracellular EGTA concentration was increased. The above results could be concluded that these current components strongly suggested as a Ca^2+ -dependent Cl^- current in the gastric antral smooth muscle cells.