Diatomaceous earth를 이용한 경제적이고 효율높은 homemade PCR purification column의 제조 및 평가

이동호,이승관,이창규,조경진,최명재,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

In recent years the amount of sequencing has increased tremendously, especially due to the introduction of automated DNA sequencing. PCR is principal method for the synthesis of templates for DNA sequencing. Consequently, the need for PCR cleanup for template preparation has increased. Already, many PCR purification kits are marketed. This DNA purification kits uses most glass membrane or silica gel, So, can do rapidly efficient purification by simple miniprep. But, efficiency of purification kits is different according to manufacturer and is very expensive. Therefore, we compare the homemade Diatomaceous earth column with major manufacturer's spin column methods for DNA purification. As a result, we found that the homemade Diatomaceous earth column method resulted in equal or better yields relative to the commercial purification kits.

COX 모델을 이용한 생존자료분석에서 Proportionality 가정 검정의 의의

이무송,유근영,노동영,최국진 대한암학회 1991 Cancer Research and Treatment Vol.23 No.4

고속핵에서 GABA가 동맥압에 미치는 영향

이정헌,오기화,안동국,박재식 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : NTS (nucleus tractus solitarius)는 압감수성 반사 구심성 신경이 처음 시냅스를 형성하는 곳으로 동맥압 조절에 중요한 역할을 한다. NTS에서 어떤 특정 물질이 동맥압 조절에 관여하는지는 정확히 밝혀지지는 않았지만, NTS에 존재하는 많은 물질들이 이들 과정에 관여하고 있다. 그중 하나인 GABA성 약물이 NTS에서 순환계에 미치는 영향을 구명하고자 본 실험을 시행하였으며 아울러 반대편 NTS를 lidocaine으로 억제하였을 경우의 변화를 관찰하여 NTS에서 신경회로 일단을 밝히고자 하였다. 대상 및 방법 : 실험동물은 체중 300-500g 되는 숫컷 Wistar쥐를 사용하였다. 동맥압 측정과 약물 주입을 위하여 대퇴 동정맥에 삽관하였다. 이어서 기관지절개술을 시행하였으며 체온을 36-37℃ 되게 열판을 이용하여 조절하였다. 쥐를 뇌 정위 고정 장치에 두고 머리를 45°숙여 NTS를 노출시킨 다음 약물을 10초간에 걸쳐 80nl주입하였다. 결과 : 인공 뇌척수액을 주입하면 동맥압의 변화가 거의 없었으나 동량의 GABA를 NTS에 주입하면 동맥압이 증가하였다. GABA에 의해 동맥압이 증가된 상태에서 반대측 NTS에 lidocaine을 주입하여 한쪽 기능을 억제시키면 동맥압이 더욱 증가하였다. 또한 lidocaine주입 후 반대쪽의 건전한 NTS에 GABA를 재차 주입하면 동맥압 증가가 적었다. 그리고 GABA를 NTS에 주입 후 정맥으로 nitroprusside를 주입하면 GABA 주입이 없는 대조군에 비해 동맥압 감소가 더 컸으며, lidocaine으로 마취하여 한쪽 NTS의 기능을 차단한 후 반대쪽 건전한 NTS에 GABA를 주입한 경우는 동맥압 감소가 적었다. 동일한 방법으로 lidocaine을 주입한 후 phenylephrine으로 승압을 시키면 동맥압 증가가 적었다. 압감수성 반사는 NTS에 GABA를 주입하거나 또는 반대편 NTS를 lidocaine으로 마취하여 기능을 억제시킨 경우에 둔화되었다. 결론: NTS에서 GABA가 동맥압의 조절 인자로 작용하며, 양쪽 NTS는 상호작용하고 있는 것으로 보인다. The nucleus tractus solitarius (NTS), the site of termination within the brain of baroreceptor afferent fibers, has been shown to be involved in the regulation of arterial pressure (AP). Many neuroactive substances present within the NTS have been shown to be involved in this process, although the manner in which any specific neurotransmitter acts physiologically in the regulation of AP has not been completely elucidated. The present study was intended to clarify the mechanism of action of GABA in the NTS and also to examine the cardiovascular response to inhibition of the contralateral NTS by lidocaine. Male Wistar rats weighing 300-500 g were employed for the experiment. The femoral artery and vein were cannulated to record the arterial pressure (AP) and to inject nitroprusside or phenylephrine, respectively. The tracheotomy was performed and body temperature was kept to 36-37℃. Rats were mounted in a stereotaxic frame with the head flexed downward to an angle of 45°. After the NTS was exposed. GABA or lidocaine was microinjected into the NTS in a volume of 80 nl over 10 seconds through a glass micropipette (outer diameter of the tip 50 ㎛) connected to a 1 ㎕ Hamilton microsyringe. The results obtained are summarized as follows. Microinjection of artificial CSF into the ipsilateral NTS did not alter basal AP. In contrast, microinjection of the same volume of GABA into the NTS produced a significant increase in the AP. The results presented thus far indicated that the volume injected did not affect AP. In animals with elevated AP by GABA injection, injection-of lidocaine into the contralateral NTS resulted in a further increase in AP. After the contralateral NTS was inhibited with lidocaine. injection of GABA into the NTS produced a less increase in AP. After microinjection of GABA into the NTS, the nitroprusside administered i.v. accentuated the increase of AP but not after microinjection of lidocaine into the contralateral NTS. Similar results were obtained with i.v. injection of phenylephrine after pretreatment of lidocaine into the NTS. The baroreflex sensitivity was reduced by microinjection of GABA into the NTS or microinjection of lidocaine into the contralateral NTS. These results suggest that alterations in GABA-mediated neural transmission in the NTS contribute to the regulation of AP and the NTS of both sides have a mutual interaction.

신부전이 동반된 당뇨병 환자에서 발생된 침습성 모균증 : 수술과 Liposomal amphotericin B 및 GM-CSF 병합 요법에 의한 성공적인 치험 1예

이원영,오기원,임국희,장재혁,이동건,최정현,강무일,신완식,차봉연,이광우,손호영,강성구 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.4

저자들은 신부전이 동반된 당뇨병 환자에서 발생된 부비동형 모균증에 대하여 수술과 함께 liposomal amphotericin B 및 GM-CSF의 복합치료를 하여 성공적으로 치료한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Mucormycosis (zygomycosis) primarily affects diabetic or immunocompromised patients and typically progresses rapidly, necessitating surgical excision and antifungal therapy with amphotericin B. Large doses of amphotericin B are needed for cure, but it has the risk of causing significant renal toxicity. The recent development of liposomal amphotericin B allows antifungal therapy to be administered with potentially improved efficacy and reduced nephrotoxicity. We have experienced a case of paranasal mucormycosis successfully treated with surgery, liposomal amphotericin B and GM-CSF. A 59-year-old male suffering from diabetes mellitus for 6 years was admitted with pain at left maxillary area. He was diagnosed as mucormycosis after cytologic exam on the necrotic nasal mucosa, which showed typical hyphae. He have had diabetic nephropathy with macroproteinuria and had rapidly rising serum creatinine levels with the amphotericin B treatment: creatinine levels reverted to basal level with the use of liposomal amphotericin B. Despite surgical excision and continued antifungal therapy, his infection was not effectively controlled. Therefore, GM-CSF was administered additionally to improve phagocytic activity of leukocytes. He was finally cured after receiving a combination of aggressive surgery, liposomal amphotericin B and GM-CSF. To our knowledge, this is the first detailed clinical description of the treatment of mucormycosis with liposomal amphotericin B in Korea.

Zeolite X 촉매를 이용한 WO₃의 CO 환원-탄화반응

이동활,이종대,전진혁,박노국,류시옥,이태진 嶺南大學校 工業技術硏究所 2003 工業技術硏究所論文集 Vol.31 No.2

This study was performed to manufacture tungsten carbide from CO reduction- carburization of WO₃using zeolite-NaX catalyst. Zeolite-NaX catalyst could improve the reduction property of WO₃. Moreover, zeoite-HX and KX catalysts also improved the reduction property of WO₃. This improvement of reduction property leaded to the decrease of reaction temperature. The size of WC particle was 50∼60 nm at 650 ℃ for 10h. From these results, it is concluded that the various zeolite X catalysts could be used for the reduction-carburization of W0₃ as the catalysts.

WO_3의 환원-탄화에 있어서 Zeolite NaX, HX, KX의 특성에 따른 영향

이동활,이종대,박영효,박노국,이태진 한국공업화학회 2003 응용화학 Vol.7 No.1

The effect of the Zeolite HX,NaX, and KH prepared by ion-exchange method, on the reduction-carburization of WO_3 was studied This study was performed at thetemperature range from 300℃ to 735 ℃ with CO gas. The produced gas, CO_2, was analyzed by mass spectroscopt The addition of the Zeolite HX, NaX,and KX into WO_3 increased the efficiency of the reduction-carburization of WO_3 and Zeolite HX with the strongest acidity was the most effective on redution-carburization among them. The Zeolite HX, NaX, and KX improved the oxygen mobility of WO_3 and the acidity of the Zeolite was directly related to the increasing efficiency of reduction-carbuization of WO_3.

대학 병원을 방문한 만성 일상성 두통 환자의 특징

도진국,오희종,이동국 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

실신으로 발현된 기립성빈맥증후군 : 단일 기관 예비 연구

이동준,김종국,허소영 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background and Objectives : Postural orthostatic tachycardia syndrome (POTS) is characterized by excessive tachycardia without prominent hypotension in the upright position. Most of the patients experience symptoms of orthostatic intolerance such as lightheadedness, dizziness, palpitation, nausea, or headache. There were limited POTS cases presenting as syncope thus, we analyzed syncope patients who showed orthostatic tachycardia in tilt table test. Methods : A single center retrospective analysis was done for patients with POTS who developed syncope. We reviewed their clinical records and results of autonomic function tests (sympathetic skin response, heart rate variability test to hyperventilation, heart rate variability test to Valsalva maneuver, 15:30 ratio and blood pressure and heart rate response to tilt table test). Results : There were 13 patients of POTS with syncope. Mean age of onset was 22.3 (14-38) years old. Men were eight and women were five. In autonomic function tests, mean orthostatic heart rate increments were 35.2 beats per minutes (bpm). Mean maximal heart rate within 10 minutes and the time for maximal heart rate were 99.9 (87-128) bpm and 7 minutes, respectively. Mean systolic blood pressure was not changed significantly during tilt table tests but, mean diastolic blood pressure was increased as much as 14.4 (±8.0) mmHg compared with supine position. Conclusions : POTS patients who manifested assyncopal attack showed similar patterns in clinical characteristics and autonomic function tests compared with ordinary POTS reported previously. These suggest that syncope can be one of the leading features of POTS. This preliminary study suggests that structured and controlled studies about POTS and syncope are needed.

日本 民族主義의 성격

李瑛雨,金東國 경북대학교 평화문제연구소 1993 평화연구 Vol.18 No.-

다단계 반응 및 단일 화염 표면모델을 이용한 석탄입자의 연소특성에 관한 수치적 연구

이창형,박원희,민동호,김태국 중앙대학교 기술과학연구소 2000 기술과학연구소 논문집 Vol.30 No.-

석탄 연소장치의 설계나 석탄 연소의 최적 조업조건 등을 추정하기 위하여 석찬 연소에 관한 많은 연구가 진행되어 왔지만 석탄 자체의 특성인 휘발분, char 그리고 회분 등의 분율과 성분에 따른 복잡성 때문에 일반적인 석탄의 연속특성을 규명하기란 쉬운 일이 아니다. 본 연구에서는 석탄의 수분 증발, 휘발, char 연소 등으로 진행되는 연소과정을 수치해석적으로 Modeling 함으로써 고체연료의 연소 특성을 파악하였다. 석찬의 종류, 크기, 주변기체의 온도, 유속, 방사율, 그리고 이산화탄소와 산소의 질량분율에 따른 연소특성을 파악한 결과 입자의 크기가 클수록, 주변기체의 유속이 빠를수록 연소가 빨리 진행되며, 주변기체의 온도와 방사율, 그리고 이산화탄소와 산소의 질량분율이 클수록 연소 진행이 빨라짐을 알 수 있다. 그러나 이산화탄소와 산소의 질량분율은 휘발분의 휘발에 거의 영향을 미치지 않음을 알 수 있다. 따라서 본 논문에서 고려한 인자들은 고체연료를 사용하는 연소시스템의 설계에 중요한 영향을 미치는 인자임을 알 수 있다. A systematic study on coal combustion is needed to design the coal combustion systems and to search for the best operating condition. In this study, we studied the coal particle combustion by considering the evaporation of moisture, two-reaction model of daf coal and heterogeneous carbon reaction of char which could be observed during the coal combustion. To study on the characteristics of the coal combustion, types and sizes of coal, temperatures and velocities of gas around the coal particle, emmisivities and concentrations of CO_2 and O_2 were considered for the parametric study. The rate of combustion is observed to be faster for smaller particle sizes and for faster gas velocities. As the temperature, emissivity and concentrations of CO_2 and O_2 of the environment become higher, the coal combustion rate becomes faster. However, the concentrations of CO_2 and O_2 didn't appreciably affect the devolatilization of the daf coal. From the result of this study, the parameters considered in this study can be important for the design of various coal combustion systems.