Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

침습성 아스페르길수스증 진단을 위항 갈락토만난 항원검사의 유용성 평가

송경호,이신원,장희창,전재현,박완범,박경운,박상원,오명돈,최강원 대한감염학회 2009 감염과 화학요법 Vol.41 No.2

Background : Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised patients. However, 1A is difficult to diagnose, especially in the aforementioned patient group. Recently, galactomannan assay (GMA) using enzyme immunoassay has been introduced in Korea. We evaluated its diagnostic usefulness in the diagnosis of 1A and we analyzed the results according to the underlying diseases. Materials and Methods : All patients who underwent GMA during the period from October 2007 to June 2008 were evaluated retrospectively. According to the criteria of European Organization for Research and Treatment of Cancer/Mycoses Study Group, IA was classified into four clinical categories: proven', probable', possible', and 'non' IA. Patients with 'proven' and 'probable' IA were used as the reference standards for IA. GMA was performed using Platelia Aspergillus EIA (Bio-Rad, Hercules, CA, USA). Optical density index ≥0.5 was considered positive. Results : Of the 144 patients who underwent GMA, two patients were classificed as proven' IA and sixteen patients were probable' 1A. Overall sensitivity, specificity, positive predictive value, and negative predictive value of the assay were 50% (95% confidence interval [C195), 26-74%), 88% (C195, 81-93%), 38% (CI95, 19-59%), and 93% (CI95, 86-97%), respectively. Among the 99 patients with underlying hematologic diseases, GMA showed 67% (C195, 35-90%) sensitivity and 89% (C195, 80-94%) specificity, whereas in 45 patients with underlying diseases other than hematologic diseases, sensitivity and specificity of the assay were 17% (CI95, 0-64%) and 87% (CI95, 73-96%), respectively. Conclusions : GMA showed high specificity, irrespective of the patient population. However, sensitivity of GMA was low and the assay was less sensitive in patients with underlying diseases other than hematologic diseases than in patients with hematologic diseases.

조직공정성과 조직후원인식의 관계에 관한 연구

정범구,김경재 충남대학교 경상대학부설 경영경제연구소 1997 경영논집 Vol.13 No.2

본 연구는 조직공정성과 조직후원인식의 상호관계를 알아보기 위해서 조직공정성과 조직후원인식의 개념과 이론을 정리하였고 연구모형과 가설을 통해서 실증분석결과를 제시하였다. 연구결과 조직공정성은 조직후원인식에 정적인 영향을 미치는 것으로 나타났고 조직공정성이 종속변수에 대하여 미치는 직접효과보다 조직후원인식을 매개로 하였을 때의 매개효과가 더 큰 것으로 나타났다.

금속광산 폐석 빛 폐재의 활용을 위한 특성 연구

민경원,진호일,최성범,조현순 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

이 연구는 상동광산 주변에 적치되어 있는 폐석과 폐재 자원을 부가가치가 높은 건설자재와 새로운 건설소재 및 첨단산업소재로 활용하기 위하여 폐석 및 폐재에 대한 암석학적 및 물리적·화학적 특성을 규명하고자 하였다. 상동광산 주변의 폐석은 주로 석회암과 셰일, 호온펠스 등이며, 폐재적치장에 쌓여 있는 폐재는 주로 석영과 정장석, 각섬석, 백운모 등의 광물로서, 구폐재 적치장의 폐재가 신폐재 적치장의 폐재보다 약간 더 조립질이고 알카리성을 나타내었다. 폐재의 주성분 원소중 MgO 함량은 신폐재에서, P₂O5는 구폐재에서 각각 뚜렷하게 더 높은 값을 보여 주었으며, 질산과 과염소산의 혼합산에 의한 부분분해에 의해 Al₂O₃, Fe₂O₃(T), MnO, MgO, K₂O, Na₂O 등은 비교적 많은 함량이 추출되었으나, TiO₂, CaO, P₂O5 등은 거의 부분 추출되지 않았다. 폐재의 pH가 증가할수록 Fe₂O₃(T)는 감소하는 경향을 나타내었고, 작열감량(L.O.I.)은 CaO와는 높은 양의 상관관계, SiO₂와는 높은 음의 상관관계를 보여 주었다. The purpose of this study is to determine the petrographic, physical and chemical characteristics of waste rock and sludges around Sangdong mine in order to utilize them as new and high quality construction materials. The waste rocks piled around Sangdong mine are mainly composed of limestone, shale and hornfels, and the sludges in sludge-piles are mainly composed of quartz, orthoclase, amphiboles and muscovite. The sludges in old sludge-piles show a tendency of coarse grains and alkaline than those in new sludge-piles. The sludges of new sludge-piles contain significantly higher abundances of MgO, and those of old sludge-piles show significantly higher content of P₂O5. In consideration of total extraction contents for sludges, the partially extracted contents of Al₂O₃, Fe₂O₃(T), MnO, MgO, K₂O and Na₂O by mixing acid of nitric and perchloric acid, are relatively high, and those of TiO₂, CaO and P₂O5 are very low. According as pH increases, abundance of Fe₂O₃(T) in the sludges of Sangdong mine decreases. L.O.I.(loss on ignition) shows highly positive and negative correlations with CaO, and SiO₃, respectively.

Nd:YAG 레이저 조사에 의한 근관벽의 색소 참투도 및 표면 변화에 관한 연구

이경범,신동훈 大韓齒科保存學會 1996 Restorative Dentistry & Endodontics Vol.21 No.1

급성 지주막하 출혈에 동반된 관상동맥 연축에 의한 심근경색증 1예

부귀범,박근호,양종태,이동민,장성종,김건영,장경식 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

In many cases, acute cerebrovascular disease is accompanied by abnormal electocardiograms. Also, though rather uncommon, acute myocardial infraction is also seen. The mechanism of its occurance is uncertain but it is thought to be related to coronary artery spasms, an abnormal autonomic nervous system, and a catecholamine increase. A female patient of 34 years of age came to the hospital because of sudden headache accompanied by substernal chest pain. A subarachnoid hemorrhage due to a cerebral aneurysm rupture had occured, and there was ST segment elevation in lead II, III, and aVF of the electrocardiogram. A cardiac enzyme test, an echocardiography, and a coronary angiography were performed, and she was diagnosed to have acute myocardial infarction due to coronary artery spasm. Clipping of the aneurysm was performed. With the use of nitrate and an angiotension converting enzyme inhibitor, the condition of the patient improved.

현무암 석분 활용에 대한 연구

민경원,진호일,최성범,박진동 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

철원지역에서 발생되는 현무암 석분은 주로 휘석과 사장석, 방해석, K-장석 등으로 구성되어 있고 건조한 상태에서 회색을 띄며 참비중은 2.86이고 94 vol.%이상이 미사질이나 모래질 입도로 이루어져 있다. 현무암 석분은 pH 8.3인 약알카리성으로서 중금속 용출시험 결과 환경보전법에서 설정한 Cu, Pb, Zn, Cd, Cr, As, Hg 등 6가지의 중금속 원소가 모두 기준값 이하의 낮은 용출 함량을 보여 농지개량을 위한 객토용으로서의 충분한 이용 가능성을 나타내었다. 현무암 석분은 광물학적 및 물리적·화학적 특성과 폐기물인 석분을 다량 소모시키면서 2차적인 석폐기물을 발생시키지 않아야 한다는 점등을 고려할 때, 결합재의 배합비를 석분에 대한 무게비로 대략 45∼50wt% 정도로 하여 폴리머 복합소재를 이용한 캐스트 제품을 만들어 상품화하는 것이 바람직할 것으로 판단되며, 이를 위해서는 지역 특성에 맞는 다양한 캐릭터 및 몰드 제작은 물론 캐스트용 제품의 고기능화에 대한 지속적인 연구가 요구된다. The sludges mostly of silt or sand size grains (above 94 vol.%) from the Cheolwon district are mainly composed of pyroxene, plagioclase, calcite and K-feldspar, and showed gray colors in dried state. Their true specific gravities and pH's are 2.86 and 8.3, respectively. The sludges from the Cheolwom district have less leached contents of six heavy metals (Cu, Pb, Zn, Cd, Cr, As and Hg) than those of standard levels established in the Soil Environment Conservation Regulation, which impliess that the sludges can be utilized as modifier of soil nature. In consideration of mineralogical, physical and chemical properties and effects of consuming the basalt sludges without secondary stone wastes, some character products had been developed by utilizing the basalt powder sludges with adding binder (unsaturated polyester resin) in 45∼50wt%. Further studies on development of regional character, commercialization of cast products, manufacture of mold and etc. are recommended for more effective utilization of basalt powder sludges.

Diazepam과 Lidocaine이 노인환자의 기관내삽관시 심전도 및 심맥관계에 미치는 영향

김경희,권성범,신용섭,최세진 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

We selected at random 30 geriatric patients who had received operation under the general anesthesia with endotracheal intubation. The patients were divided into 3 groups. Group 1 (control) had received endotracheal intubation without diazepam and lidocaine. Group 2 had received endotracheal intubation with diazepam. Group 3 had received endotracheal intubation with lidocaine. The changes of arterial blood pressure and pulse rate, and the appearance of arrhythmia were compared in each group. The results were as follows : 1. The increase of blood pressure and heart rate were less in group 2, 3 than the control group which showed significant increase of blood pressure and heart rate with endotracheal intubation. 2. The incidence of arrhythmias with endotracheal intubation was less in group 2(20%), group 3(50%) than the control group(80%). 3. The electrocardiographic change in the group 2 and 3 were not significant compared to group 1(control).

학습조직 구축요인과 조직특성의 교육훈련 효과성 연구 : 콜센터 종사자를 중심으로

김경재,정범구,조장현 충남대학교 경영경제연구소 2008 경영경제연구 Vol.31 No.2

With the development of telecommunication technology, and with combination of computer and communication, a new field, which is called a customer care call center , has been created. In the past, the customer center was just passive in the way that their role was just answering to the customers' questions. However, now this has been changed to be active and positive for fulfill customers' satisfaction. Therefore, not only enterprises, but also public institutions and government agencies are having customer care centers as one of methods to maximize customer service. Also, the role of customer care center is extended to marketing activities. The actual proof study on training of call center employees and the transfer of the training to the work has been practiced for this research. The findings of this research are summarized as follows ; first, building factors of learning organization is positively associated with the transfer of training, second, organizational characteristics has moderating effects between building factors of learning organization and training effectiveness. 본 연구는 콜센터 종사자를 대상으로 학습조직 구축요인과 조직특성의 교육훈련 효과성 관계를 알아보기 위하여 학습조직구축요인, 조직특성, 교육훈련 효과성의 개념과 이론을 정리하였고, 연구모형과 가설을 통해 실증분석결과를 제시하였다. 연구결과 학습조직 구축요인이 교육훈련 효과성에 영향을 미치는 것으로 분석되었고, 양 관계에 있어서 조직특성이 부분적으로 조절효과가 있는 것으로 나타났다.

뇌실내출혈로 인한 급성 수두증에서 관찰된 해리증후군

김광기,이경민,이정주,오종배,안태범,정상욱,남현우,박성호 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5