독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

노인들의 생활체육참가가 건강체력과 행복감에 미치는 영향

전종옥,함주현,박승한 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of this study was to how the sports for all participation the effect on health fitness and happiness of in elderly. This study is with selected in 20 sport for all of participation people 20, not participation 20. The measurement items were sit and reach, 800M, body fat %, grip strength and happiness. The data statistics was applied by one-way ANOVA in SpssWin8.0. The result are as follows; 1. There were statistically significant difference between the grip strength, 800M, body fat % and sit and reach means of the control group and the experimental group. 2. The happiness was participation people higher than gruop not participation

과학교사에 관한 선행연구 분석

우종옥,정진우,홍성일 한국교원대학교 과학교육연구소 1995 청람과학교육연구논총 Vol.5 No.1

There were many studies on the science teacher. Also, there were many researches on the science teacher education. What seems to be needed is more analysis and synthesis of these results. In this study, the results of preceding-research were analyzed and categorized. The study subjects were preceding-researches published on the 'Journal of the Korean Association for Research in Science Education' and the 'Science Education' of America. The results of this study were as follows: The trend of research on science teacher and teacher education became more various and diverse. The domain of research were categorized in three: (1) Teacher Character(of Cognitive and Affective), (2) Teacher Education(of in-service and pre-service), (3) Professional Practice (of Teaching). The implication of this study was that it was more necessary to investigate on the teacher's teaching strategies.

‘께서, 에서, 서’의 문법 기능에 대하여

김수정(Kim, Su-Jeong),정경옥(Jung, Kyung-Ok),서종학(Suh, Jong-Hak) 한국어문학회 2014 語文學 Vol.0 No.126

The purpose of this study lies in clarifying the grammatical function of Korean particles ‘kkeseo, eseo, seo(께서, 에서, 서)’ and establishing their grammatical position. In addition to their features discovered in a number of discussions, it is claimed that through the analysis of their syntactic structure and passive sentences, ‘kkeseo, eseo, seo(께서, 에서, 서)’ are revealed to play a role of the subjects of noun phrase. However, ‘kkeseo, eseo, seo(께서, 에서, 서)’ are defined as auxiliary particles rather than nominative particles.

스쿼시운동이 여대생들의 건강관련체력 ㅣ및 혈청지질에 미치는 영향

박익종,양정옥 신라대학교 자연과학연구소 2007 自然科學論文集 Vol.16 No.-

The purpose of this study was to investigate the effects of squash exercise program on health-related and serum lipid in female university students. The participants of the study were 14 female students, and they were divided into the squash(SG, N=7) and control group(CG, N=7). To evaluate physical fitness and serum lipid analyzed before and after the intervention. Squash exercise programs lasted for 12weeks in duration, 3times a week, and 60 minutes in every session. A pained t-test were used for data analyses, with the critical value of .05. The results of this study were as follow: 1. % of body fat were significantly decreased after the intervention in the SG group. Cardiorespiratory endurance and muscular strength/ endurance were significantly improveds in the SG group. 2. The level of TC, LDL-C, HDL-C, and AI was significantly and positively, changed, but that of TG was not. Results also indicated that both the SG group were superior to the CG after the intervention. Comparing exercise program, no significantly differences were found between the SG group, except for muscular endurance in which the SG was better than group. It was concluded that both the squash exercise coupled program can be beneficial for young female college students.

입제농약을 활용한 벼 병해충 생력방제 연구

김정수,이종옥,강수웅,김재현 진주산업대학교 농업기술연구소 2003 農業技術硏究所報 Vol.16 No.-

The experiment was carried out to establish of labor-saving control system on paddy field transplanted young rice seedling by machine. We examined the effect of control value between conventional control method that applied 6 times to paddy field during cultivation period and labor-saving control method that applied systematic pesticide one time to the seed, to the nursery box at transplanting day and twice to the middle stage of rice cultivation. The control value in labor-saving control method of Laodelphax striatellus, Nephotettix cincticeps and Lissorhoptrus oryzophilus were 93.4%, 92.7% and 86.1% compared with untreatment field, respectively and also the labor-saving method was more efficient than conventional control method at early stage of rice cultivation. The control value in labor-saving method of Nilaparvata lugens, Sogatella furcifera, Cnaphalocrocis medinalis were more than 84% and these showed almost same trend with conventional control method. The control value in labor-saving method of leaf blast and panicle blast were 91.7%, 93.8%, that showed similar result with conventional control method. The control value in the nursery box at transplanting day of sheath blight was 83.6%, these result were better than conventional control method that applied 2 times at middle stage of rice cultivation.

야생차의 생육특성에 관한 연구

고영옥,이종옥,최주호,정영섭,이용호 진주산업대학교 농업기술연구소 2003 農業技術硏究所報 Vol.16 No.-

본 시험은 최근 각광 받고있는 차나무의 우량품종 육성과 재배에 관한 기초자료를 구명코자 2001년 3월부터 2002년 10월까지 남부지방 자생차의 지리적·생태학적 특성 조사와 RAPD 유연관계를 비교 분석하였으며 그 결과는 다음과 같다. 1. 녹차 자생지 토양의 pH는 4.1 ~ 6.2로 내대리가(pH 6.2) 가장 높았으며, 다사리가 pH 4.1로 가장 낮았다. 2. P_(2)O_(5)는 농업기술원과 다솔사 차가 319㎎/㎏ 이상으로 가장 높았고, 송광사와 다압리는 20㎎/㎏ 이하로 가장 낮았다. 3. 차 포장의 지형은 혜산서원, 농업기술원을 제외한 대부분 자생지가 해발 220m 이상의 산간지 였으며, 북서풍을 막을 수 있는 남향 또는 남서방향이었다. 4. 순천시 송광사, 사천시 배방사, 다솔사, 밀양시 혜산서원, 고성군 옥천사, 남해군 보리암 차엽은 yabukita 보다 대엽이었다. 5. 엽장은 다솔사가 13.9㎝로 가장 길었고 도심다원은 5.2㎝로 가장 짧았으며, 엽폭은 배방사가 4.9㎝로 가장 넓었으며 매암다원이 2.1㎝로 가장 좁았다. 6. 거치수는 배방사가 79개로 가장 많았으며 천은사가 50개로 가장 적었다. 7. 증폭된 DNA의 band pattern을 비교해 보면 절편 크기는 300~3000bp사이였고, 지역별 band 영상은 뚜렷하였다. 8. Primer별 평균 band수는 7.6개였으며, 22개 Primer당 전체 band 수는 76~192개 범위였다. 9. RAPD UPGA에 의한 유연관계 분석을 위한 similarity matrix값에 근거하여 유연성 분석을 실시한 결과 차가 유전적 거리지수 0.10이내에 우선적으로 유집되었으며, 고성군 옥천사, 밀양시 혜산서원, 엄광사 수집종은 0.25의 유전적 거리지수로 유집되었으며 모두 대엽이었다. This study was carried out to investigate the geographical and ecological characteristics of self-sown teas in the southern southern part of South Korea and evolutionary relationship based on the RAPD analysis data. Each sample was collected from March, 2001 to October, 2002 along with basic characteristics of its cultivation. The results are described as follow : 1. The pH of soils in the habitat of collected green teas were 4.1~6.2. The highest pH(6.2) was shown from the soil of Naedae-ri while that of Dasa-ri showed the lowest pH value. 2. The highest content (319㎎/㎏) of P_(2)O_(5) was examined from the tea of the Agricultural Research Extension Services (ARES) while the lowest values were obtained from the collected samples of Shonggwansa and Dasa-ri (20mg/kg). 3. In terms of geographical aspects, most of the areas that samples were collected are mountains with 220 meters above the sea level and are in the direction of the southern exposure and the southeast except Hyesan lecture hall and ARES.4. In Shonggwangsa of Shunchon-si, Baebangsa and Dasolsa of Sacheon-si, Hyesan lecture hall of Miryang-si, Okcheon-sa of Goseong-gn and Boriam of Namhae-gn, the self-sown teas were a kind of bigger size of leaf than that of Yabukita. 5. The tea of Dasolsa had the longest leaf of 13.9㎝, but the tea of Dosim tea plantation had the shortest leaf of 5.2㎝. Baebangsa showed the broadest leaf width of 4.9㎝, and Maeam tea plantation showed the narrowest leaf width of 2.1㎝. 6. The tea of Baebangsa had 79 serrations of the largest number but Cheoneunsa had 50 serrations of the smallest number. 7. When the band pattern of the amplified DNA by PCR were compared, a size of the bands was between 300 and 3,000bp. The band image by area of gathering seeds was distinct. 8. There were the average number of 7.6 bands per primer. The number of total bands per 22 Primers were between 76 and 192 bands. 9. The result values of a flexibility analysis based on a similarity matrix value for the flexible relation analysis by RAPD UPGA, were attracted within 0.10 of inherited distance index. The collected species in Okcheonsa of Goseong-gn, Hyesan lecture hall and Eomgwangsa of Miryang-si were attracted within the inherited distance index of 0.25 and were all the kind of the big leaf.

폐결핵 환자의 우울 상태

홍정표,최선희,조만종,이성주,박종,김기순,김양옥 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.1

The main purpose of this study is to identify and evaluate the depressive states for the patients with chronic pulmonary tuberculosis. A questionnaire(Self-rating Depression Scale, SDS) survey was performed toward 57 pulmonary tuberculosis patients with initial treatment who started chemotherapy and 53 pulmonary tuberculosis patients with retreatment, admitted to one tuberculosis-centered hospital and two general hospital. Fifty three healthy persons as control group were investigated with the same questionnaire for comparison. The survey was performed from July to August 1996. The results are as follws : 1. The patients had significantly high scores in the depression than control and the patient with retreatment showed significantly high scores in the depression than the patient with initia1treatment(P<0.01). 2. The depressive levels of the patients were associated with duration of admission and the expectancy of recovery (P<0.05). 3. The percentage of the subjects with 40 or more scores tentatively classifiable as psychologically depressed was 67.1% of the total subjects, 48.4% of the control groups, 75.0% of the patient with initial treatment and 84.1% of the patient with retreatment. Because patient with pulmonary tuberculosis with initial treatment showed higher depression scores than normal persons, and patients with retreatment due to failure of initial treatment showed higher depression scores than initial treatment, it is necessary to give attention to depression of the patients during treatment of pulmonary tuberculosis.

구조화된 문항을 이용한 지구과학 탐구능력 평가 도구 개발

정철,우종옥,김정률 한국교원대학교 과학교육연구소 1997 청람과학교육연구논총 Vol.7 No.1

The purpose of this study is to develop a test of earth science inquiry abilities using structured items. To develop a more valid and reliable instrument factual data was used, and most of the previous studies regarding inquiry teaching method and inquiry evaluation were consulted. The model developed in this study is composed of 5 stages; recognizing problems and generating hypotheses, designing inquiry, pursuiting inquiry, interpreting data, and drawing conclusion. Also, in this study 10 science inquiry abilities; recognizing problems, generating hypotheses, controlling variables, designing experiments, performing experiments, transforming data, inferencing, predicting, conclusing, and generalizing were clearly defined. The test developed in the study, Test of Earth Science Inquiry Abilities, includes 25 multiple-choice (five-choice) items and requires testing time of 50 minutes. The content validity of items, objectivity of scoring keys and clarity of items were checked by 7 experienced specialists in science education and earth science. The developed test were investigated and revised through three field tests. According to the results of the third field trial, test reliability (Cronbach α) was 0.62, difficulty index was 0.54, and index of discrimination was 0.35. Also, the developed Test of Earth Science Inquiry Abilities showed a correlation coefficient of 0.53 with TIPS. Therefore, the development of Test of Earth Science Inquiry Abilities using structured items satisfied the reliability and validity requirements for general assessment instruments for students' earth science inquiry abilities.

活性汚泥 微生物의 分離 및 同定

강명규,배정옥,이기종,이영하 忠南大學校環境問題硏究所 1993 環境硏究 Vol.11 No.-

To investigate the microfloral composition in activated sludge, more than six hundred bacterial and fungal strains isolated from influent sewage, return sludge and aeration tank of an activated sludge sewage treatment plant were identified to the genus level. During the holding period in the aeration tank development of diverse heterotrophic microbial populations occurred and bacterial and fungal numbers in the aeration tank were 10- to 100-fold higher than in the influent sewage and return sludge. A large number of Gram-negative rods, particularly Pseudomonas, Flavobacterium, Enterobacter, Klebsiella, and Alcaligenes were found to be the dominant genera in the aeration tank. The species of filamentous fungi Trichoderma, Aspergillus, Rhizopus and Lepiomitus, and various yeasts were frequently associated with activated sludge. The most commonly found yeast genera were Candida, Geotrichum, Saccharomyces, Torulopsis and Rhodotorula.