Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice

Yoon, Hye Eun,Kim, Eun Nim,Kim, Min Young,Lim, Ji Hee,Jang, In-Ae,Ban, Tae Hyun,Shin, Seok Joon,Park, Cheol Whee,Chang, Yoon Sik,Choi, Bum Soon Hindawi Publishing Corporation 2016 Oxidative medicine and cellular longevity Vol.2016 No.-

<P><I>Background</I>. This study evaluated whether the change in the renin-angiotensin system (RAS) is associated with arterial aging in mice.<I> Methods</I>. Histologic changes and expressions of transforming growth factor-<I>β</I> (TGF-<I>β</I>), collagen IV,<I> fibronectin</I>, angiotensin II (Ang II), angiotensin-converting enzyme (ACE), angiotensin-converting enzyme 2 (ACE2), angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), prorenin receptor (PRR), Mas receptor (MasR), endothelial nitric oxide synthase (eNOS), NADPH oxidase 2 and oxidase 4 (Nox2 and Nox4), 8-hydroxy-2′-deoxyguanosine (8-OHdG),<I> 3-nitrotyrosine</I>, and superoxide dismutase 1 and dismutase 2 (SOD1 and SOD2) were measured in the thoracic aortas from 2-month-old, 12-month-old, and 24-month-old C57/BL6 mice.<I> Results</I>. Twenty-four-month-old mice showed significantly increased aortic media thickness and expressions of TGF-<I>β</I>, collagen IV, and fibronectin, compared to 2-month-old and 12-month-old mice. The expressions of PRR, ACE, and Ang II, and AT1R-positive area significantly increased, whereas expressions of ACE2 and MasR and AT2R-positive area decreased with age. The expressions of phosphorylated serine<SUP>1177</SUP>-eNOS, SOD1, and SOD2 decreased, and the 8-OHdG-positive area and the 3-nitrotyrosine-positive area increased with age. The expression of Nox2 significantly increased with age, but that of Nox4 did not change.<I> Conclusions</I>. The enhanced PRR-ACE-Ang II-AT1R axis and reduced ACE2-MasR axis were associated with arterial aging in mice. </P>

Treatment of alopecia areata with tofacitinib

( Ji Su Lee ),( Min-woo Kim ),( Hyun-sun Park ),( Jung Yoon Ohn ),( Hyun-sun Yoon ),( Chang-hun Huh ),( Ohsang Kwon ),( Soyun Cho ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.2

Background: Treatment of alopecia areata (AA) is often empiric and not yet satisfactory. Recently, Janus kinase inhibitor showed promising results in Caucasians. Objectives: To investigate efficacy, tolerability, and hair growth trajey of oral tofacitinib monotherapy for Korean AA patients over extended period. Methods: This is a retrospective study of adult AA patients treated with tofacitinib monotherapy for least 4 months. Results: Thirty-six Korean patients with median initial SALT score of 98.9 were included; 91.7% had refractory AA. Twenty-nine (80.6%) patients demonstrated more than 5% SALT change. Twenty patients (55.6%) achieved 50% SALT change at median 7.5 months with 5 mg twice dosage. There were significant differences between SALT 50 achievers and non-achievers: duration of current episode (ρ = 0.009), duration of disease since first onset (ρ = 0.026) and age at onset of first episode (ρ = 0.011). Tofacitinib was well tolerated and there was no any serious adverse effect such as malignancy or tuberculosis reactivation. Conclusion: Oral tofacitinib monotherapy may be effective and tolerable in patients with AA. SALT 50 achievement was associated with followings: duration of current episode, duration of disease since first onset, and age at onset of first episode.

Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

Marked Decreases of Foxp3 and CTLA-4 Are Associated with Strong Antiviral Effects of Tenofovir in Patients with Chronic Hepatitis B

( Ji Young Kim ),( Chang Wook Kim ),( Yun Hui Kim ),( Seok Cheon Yeom ),( Su Gyeong Lee ),( Hee Yeon Kim ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Immune regulatory molecules such as forkhead box P3 (Foxp3) on CD4+ T cell and cytotoxic T lymphocyte-associated antigen 4 CTLA-4) on CD8+ T cell are associated with antiviral effector T cell dysfunction, which influences on T cell exhaustion and persistent viral infection in patients with chronic hepatitis B. These Foxp3 and CTLA-4 are up-regulated in chronic hepatitis B. During antiviral therapy with tenofovir, the expressions of Foxp3 and CTLA-4 could be changed. We investigated the relationship between antiviral effects of tenofovir and the expression of Foxp3 and CTLA-4 during tenofovir treatment in chronic hepatitis B. Methods: Eight patients with chronic hepatitis B under tenofovir treatment were enrolled for detection of Foxp3 on CD4+ T cell and CTLA-4 on CD8+ T cell. Peripheral blood mononuclear cells were isolated from these subjects before tenofovir treatment (T0), 3 month (T3) and 6 month (T6) during tenofovir treatment. For antiviral effect analysis, serum HBV DNA levels were checked at same time. The expressions of Foxp3 and CTLA-4 on T cells were monitored by flow cytometry. Results: Three patients (3 of 8) showed marked decreases of Foxp3 and CTLA-4 during tenofovir therapy (group 1). Five patients (5 of 8) showed minimal changes of Foxp3 or CTLA-4 during tenofovir therapy (group 2). Group 1 showed complete virologic response within 6 month therapy regardless of baseline HBV DNA level but, group 2 showed complete virologic response within 6 month therapy only in patients with low baseline HBV DNA level (< 7log HBV DNA). Conclusions: Among the patients with chronic hepatitis B, the patients who showed marked decrease of Foxp3 and CTLA-4 during tenofovir therapy are associated with strong antiviral effects of tenofovir regardless of baseline HBV DNA level. This finding suggests that restoration of HBV-specific T cell strengthens the antiviral effects of tenofovir.

공동주택 발코니 확장에 따른 결로 방지를 위한 창호 디자인의 실험적 연구

윤대원(Yoon Dae-Won),정창헌(Cheong Chang-Heon),김지영(Kim Ji-Yoeng),유선용(Yoo Seon-Yong),김태연(Kim Tae-Yeon),이승복(Leigh Seung-Bok) 대한건축학회 2008 대한건축학회논문집 Vol.24 No.10

According to the legalization of balcony expansions in apartment houses since January 2006, most of the apartment houses now are built with expanded balcony space. The existing balcony space was physically a buffer between the outside environment and the inside environment. In addition, it was a space that functioned to minimize the indoor environmental changes in relation to external weather changes. However, as apartment houses with extended balconies have become more common recently, the possibility of dew condensation increased due to the weakening of the insulation capacity as the space that can carry out these environmental buffer roles have disappeared. Also, the indoor thermal environment has been weakened. Thus, this paper seeks to resolve problems related to the expansion of balconies by presenting a new method that improves the insulation performance and prevents condensation in the window design that an apartment house with an extended balcony should have. also performance evaluation is executed on new window design to prevent condensation.

A Multicenter Analysis of Clinical Features and Long-Term Outcomes of POEMS Syndrome in Korea

Kook Hye Won,Jang Ji Eun,Min Chang-Ki,Yoon Dok Hyun,Kim Kihyun,Bang Soo-Mee,Park Yong,Lee Jae Hoon,Yoon Sung-Soo,Kim Jin Seok 대한의학회 2024 Journal of Korean medical science Vol.39 No.2

Background: POEMS syndrome is a rare form of plasma cell dyscrasia characterized by polyneuropathy, organomegaly, endocrinopathy, monoclonal proteins, and skin changes. Owing to its low incidence, there are few reports regarding this syndrome. This multicenter study included 84 patients diagnosed with POEMS syndrome in South Korea. Methods: We retrospectively evaluated 84 patients diagnosed with POEMS syndrome at 8 hospitals in South Korea between January 2000 and October 2022. The clinical characteristics and treatment outcomes were analyzed. Results: The median patient age was 53 years (range, 26–77 years), and 63.1% of the patients were male. All patients had peripheral neuropathy, and 81 (96.4%) had monoclonal plasma cell proliferation. Plasma vascular endothelial growth factor levels were available for 32 patients with a median of 821 pg/mL (range, 26–12,900 pg/mL). Other common features included skin changes (54.2%), volume overload (71.4%), and organomegaly (72.6%). Of the 84 patients, 75 received initial treatment (local radiotherapy, 6 [8.0%]; chemotherapy, 17 [22.7%]; both chemotherapy and local radiotherapy, 9 [12.0%]), upfront autologous stem cell transplantation (ASCT), 43 (57.3%; with induction chemotherapy, n = 12, 16.0%; without induction chemotherapy, n = 31, 41.3%). The median follow-up duration was 40.7 months. The 5-year overall survival (OS) was 78%, and the 5-year progression-free survival (PFS) was 55%. Patients who underwent upfront ASCT and were diagnosed after 2014 had a longer OS and PFS. Conclusion: The demographics of Korean patients with POEMS syndrome were similar to those reported previously. Because of the introduction of new treatment agents and the reduced rate of transplant-related mortality related to ASCT, the treatment outcomes of Korean patients with POEMS syndrome have improved in recent years.

위용종의 내시경적 생검과 내시경적 절제술 후 병리학적 진단의 불일치

윤원재 ( Won Jae Yoon ),이동호 ( Dong Ho Lee ),이국래 ( Kook Lae Lee ),장동경 ( Dong Kyung Chang ),김병관 ( Byeong Gwan Kim ),김지원 ( Ji Won Kim ),정지봉 ( Ji Bong Jeong ),김나영 ( Na Young Kim ),김진욱 ( Jin Wook Kim ),황진혁 ( 대한내과학회 2005 대한내과학회지 Vol.69 No.5

목적 : 위용종이란 위의 내강으로 돌출한 종괴를 의미한다. 거의 90%에서 조직학적으로 과형성 용종이며 이러한 경우 암으로의 진행은 매우 적은 것으로 알려져 있다. 그러나 위선종은 일부에서 국소적으로 암성 병변을 동반하거나, 동반하고 있지 않더라도 시간이 지남에 따라 암으로 진행하는 것으로 알려져 있다. 그러나 내시경적 겸자 생검과 전체 병변의 절제 후 조직 소견에서는 차이가 있는 것으로 알려져 있다. 저자들은 내시경검사에서 위용종이 발견된 후 내시경적 절제술을 시행받은 74명의 환자, 85예의 위용종을 대상으로 임상적 및 병리학적 특성을 알아보고 겸자 생검과 내시경적 절제술 후 병리학적 진단의 불일치를 알아보고자 하였다. 방법 : 1996년 4월부터 2003년 2월까지 내시경검사에서 위용종이 발견된 후 내시경적 생검과 내시경적 절제술 후 조직학적 진단이 모두 이루어진 74명의 환자를 대상으로 내시경 사진, 의무 기록 및 병리 기록을 분석하였다. 결과 : 74명의 환자에서 총 85개의 용종을 관찰할 수 있었다. 위용종은 전정부에서 가장 호발하였으며(58.8%) 그 다음으로는 체하부(18.8%)에 호발하였다. 내시경적 겸자 생검에서 관상 선종은 48.2%, 과형성 용종은 30.6%, 염증성 용종은 15.3%, 위저선 용종은 2.4%, 이형성증은 2.4%, 관상융모 선종은 1.1%였다. 내시경적 절제술 후 조직학적 검사상 관상 선종은 45.9%, 과형성 용종은 31.8%, 염증성 용종은 9.4%, 과오종은 3.5%, 위저선 용종은 2.4%, 관상융모 선종은 2.4%, 선암은 2.4%, 이형성증은 1.1%, 점막 위지방종증은 1.1%였다. 내시경적 겸자 생검 결과와 내시경적 절제술 후 조직학적 진단의 불일치율은 27.1%였다. 용종의 크기에 따른 진단 일치율의 차이는 없었다(p>0.05). 결론 : 위용종의 겸자 생검과 내시경적 절제술 후 조직검사 소견은 차이가 있을 수 있다. 용종의 크기는 진단 일치율에 영향을 미치지 못했다. 따라서 특히 선종이 의심되는 경우에는 크기에 상관없이 내시경적 절제술을 통한 전체 병변의 조직검사가 필요할 것으로 생각된다. Background : Gastric polyp is a descriptive term referring to mucosal prominence that protrudes beyond the flat lining of the stomach. Almost 90% of gastric polyps are hyperplastic polyps. Adenomatous polyps may contain focal carcinomatous foci or undergo carcinomatous changes. It is known that there is some degree of discordance between the results of endoscopic forceps biopsy and pathology of resected specimens. The aim of this study was to investigate the discordance in pathologic findings between endoscopic forceps biopsy and endoscopic resection specimen of gastric polyps. Methods : We reviewed endoscopic photographs and medical records of the patients who underwent endoscopic resection from April, 1996 through February, 2003. Results : A total of 85 cases of gastric polyps from 74 patients were reviewed. Male-to-female ratio was 1:1.96. Mean age was 59.9±10.8 years. Multiple polyps were observed in 10.8%. Gastric polyps occurred in the antrum most frequently (58.8%). Pathology results on resected specimens were as follows: tubular adenoma 45.9%, hyperplastic polyp 31.8%, inflammatory polyp 9.4%, hamartoma 3.5%, fundic gland polyp 2.4%, tubulovillous adenoma 2.4%, adenocarcinoma 2.4%, dysplasia 1.1%, and mucosal pseudolipomatosis 1.1%. Discordance rate between endoscopic biopsy and pathology of resected specimens was 27.1%. There was no relationship between the size of the polyp and concordance rate. Conclusions : There was considerable discordance in pathologic findings between endoscopic forceps biopsy and resected specimens. Approaches to review the histology of an entire polyp should be performed, especially when an adenoma is suspected.(Korean J Med 69:481-486, 2005)

Gene Expression Analysis of the Bromobenzene Treated Liver with Non-hepatotoxic Doses in Mice

Lim, Jung-Sun,Jeong, Sun-Young,Hwang, Ji-Yoon,Park, Han-Jin,Cho, Jae-Woo,Song, Chang-Woo,Kim, Yang-Seok,Lee, Wan-Seon,Moon, Jin-Hee,Han, Sang-Seop,Yoon, Seok-Joo The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.4

Bromobenzene (BB) is well known hepatotoxicant. Also, BB is an industrial solvent that arouses toxicity predominantly in the liver where it causes centrilobular necrosis. BB is subjected to Cytochrome P450 mediated epoxidation followed by either conjugation with glutathione, enzymatic hydrolysis or further oxidation. In this study, we focused on BB-induced gene expression at non-hepatotoxic dose. Mice were exposed to two levels of BB, sampled at 24 h, and hepatic gene expression levels were determined to evaluate dose dependent changes. When examining the toxic dose of BB treated group in other previous studies, genes related to heat shock protein, oxidative stress, and drug metabolism are expressed. Compared to these results, our study, in which non-toxic dose of BB was administrated, showed similar patterns as the toxic conditions above. The purpose of the study was to select genes that showed changes in relation to the differing dose through confirmation of the difference within transcriptomic boundaries, but those that are not detected by the existing classic toxicology tools in non-hepatotoxic dose.

나프타분해플랜트의 부탄추출공정에서 부탄증기의 연속누출에 의한 화재사고의 영향평가

윤대건,이헌창,함병호,조지훈,김태옥 韓國火災 ·消防學會 1998 한국화재소방학회논문지 Vol.10 No.4

나프타분해플랜트의 부탄추출공정에서 부탄증기의 연속 누출에 의한 제트화재와 플래쉬화재의 사고결과에 대한 영향을 평가하고 사고결과에 미치는 공정변수들의 영향을 해석하였다. 877 kPa과 346.75K의 공정조건에서 부탄증기가 87.8 kg/s의 속도로 8m의 높이에서 연속누출되는 경우에 표준조건으로 설정한 대기 및 환경조건에서 사고결과를 해석한 결과, 제트화재에서는 화염의 형태와 크기를 예측할 수 있었으며 누출지점으로부터 200m에서 API모델로 산출한 목사열은 약 5kW/m^2이었고 플래쉬화재가 일어날 수 있는 연소범위는 11.2~120.2m이었다. 그리고 사고결과에 미치는 조업압력의 영향은 조업온도보다 크며 압력이 증가할수록 사고결과 값이 증가하였다. 이때 플래쉬화재에서 X_UFL에 미치는 공정압력의 영향은 X_LFL에서보다 약 1/10이하를 나타내었다. The consequence analysis for jet and flash fire accidents by the continuous release of butane vapor was performed and effects of process variables on consequences were analyzed in standard conditions. For the continuous release(87.8 kg/s) of butane vapor at 8 m elevated height in the debutanizing process of the naphtha cracking plant operating at 877 kPa, 346.75 K, we found that for the jet fire accident, shape and size of the flame could be predicted and thermal radiation estimated by API model at 200 m distance from release point was 1.5 kW/m^2, and that for the flash fire accident, effect range was 11.2~120.2m. Also, simulation results showed that effects of operating pressures on consequences were large than those of operating temperatures and results of accidents were increased with increasing operating pressures. At this time, effects of operating pressures on X_UFL. were smaller

한국인에서 Quadruplex PCR을 이용한 8종류 상염색체 STR 유전좌의 다형성 분석

장영길,김기범,신창호,이숭덕,이윤성,이정빈 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5% polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 085 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65×10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here preyed to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.