병원간호조직의 내부마케팅요인과 간호사의 직무만족, 조직몰입과의 관계연구

김소인,차선경,임지영 고려대학교 2001 간호학논집 Vol.3 No.-

아미노 말단 영역 돌연변이 루마진 단백질들의 제조, 정제 및 특성 비교

김소영,최지선,우영은,이찬용 충남대학교 생물공학연구소 2009 생물공학연구지 Vol.15 No.-

The lux regulon from Photobecterium leiognathi contains the genes for bacterial bioluminescence reaction as well as the lumazine protein (lumP) and for riboflavin synthase (ribE) that are transcribed opposite direction. The fluorescent protein of lumazine protein is a riboflavin synthase superfamily and shows considerable amino acid identity with the enzyme. To characterize the amino acid in binding site of N-terminal domain of lumazine protein, the mutant proteins containing 6X-His tag system were constructed and purified. The mutated N-terminal domain of lumazine proteins containing one Trp amino acid at the different position show peculiar maximum wavelength peak in fluorescence emission.

혈전탄성묘사도를 이용한 만성 신부전 환자의 수술 전 혈액 응고 기능 평가

김소영,이상귀,손지선,한영진,송희선 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

Background: Patients with chronic renal failure traditionally have been recognized as being at risk for perioperative bleeding diathesis. However, there has been a few reports that chronic renal failure patients showed a hypercoagulability. The purpose of this study was to assess blood coagulation profiles in patients with chronic renal failure using a thromboelastography. Methods: Thirty patients (ASA physical status, 2 and 3) with chronic renal failure (experimental group), were scheduled to get presenting for an arteriovenous fistula formation, and 30 patients with normal renal function (control group) were randomly selected. Blood sampling for a thromboelastography was performed prior to induction of anesthesia. A thromboelastography was analyzed by measuring R time, K time, maximum amplitude (MA), α degree, and TEG index. Laboratory tests including serum blood urea nitrogen, creatinine, hematologic data (hemoglobin, hematocrit, platelet count), and coagulation data (PT, PT-INR, aPTT, BT) were also measured in all patients preoperatively. Results: In the thromboelastographic indices, K time was significantly decreased and MA, α angle, and TEG index were significantly increased in patients with chronic renal failure (experimental group) compared with the control group (P < 0.05). However, there were no significant differences of PT, PT-INR, aPTT, BT between the groups. Conclusions: Thromboelastographic data demonstrates a hypercoagulable state in patients with chronic renal failure. This finding suggests that traditional concern for bleeding diathesis in patients with chronic renal failure may require reassessment. (Korean J Anesthesiol 2002; 43: 407~412)

수준별 학습을 위한 퍼지 집합 기반 적응형 교수 시스템

최숙영,소지숙,이순정 한국컴퓨터교육학회 2003 컴퓨터교육학회 논문지 Vol.6 No.2

본 연구는 학습자 수준에 맞는 학습 내용과 평가 문제를 제공하고, 그 평가 결과에 기반하여 반복학습 및 심화학습을 효과적으로 제공하며, 차기 학습을 할 경우에 이에 기초하여 적절한 학습이 이루어질 수 있도록 하는 적응형 웹기반 교수 시스템을 제안한다. 이를 위해 코스웨어 설계시 학습목표의 중요도, 학습내용의 난이도, 학습목표와 학습내용과의 관련도에 따라 퍼지 수준 집합을 구성하고 이를 기반으로 학습자의 수준에 맞는 내용을 제공한다. 또한, 학습의 평가시에도 문제의 난이도, 평가문항과의 관련도를 고려하여 평가 결과를 제시하게 된다. 본 논문에서 제시하는 퍼지 집합에 의한 학습 내용의 제공과 평가 결과는 학습과정에 나타나는 여러 가지 다양하고 불확실한 요소들을 고려하여 처리함으로써 보다 융통성 있고 효과적인 교수 학습 방법을 제공할 수 있도록 한다. This paper proposes a web-based adaptive tutoring system based on fuzzy set that provides learning materials and questions dynamically according to students' knowledge state, and gives advices for the learning after an evaluation. For this, we design a courseware knowledge structure systematically and then construct a fuzzy level set on the basis of it considering importance of learning targets, difficulty of learning materials and relation degree between learning targets and learning materials. Using the fuzzy level set, our system offers Iearning materials and questions to adapt to individual students. Moreover, a result of the test is evaluated with fuzzy linguistic variable. Appling the fuzzy concept to the tutoring system could naturally consider and deal with various and uncertain items of learning environment thus could offer more flexible and effective instruction-learning methods.

혐오자극과 모델링 및 언어적 지시가 발달지체 아동의 상동행동에 미치는 효과 : 사례연구

한현민,함소희,김윤선,지지영,홍희영 대진대학교 교육연구소 2000 교육연구 Vol.2 No.1

The experiment was undertaken to determine the combined effects of aversive, modeling, and verbal instruction on the self-stimulatory stereotyped benavior exhibited by a three-year two-month old with developmental delay, The behavioral interventions were consisted of lemon juice(aversive), behavioral rehearsal(moleling), verbal instruction(prompting), and defferential reinforcement of incompatible behavior (DRI), and continued over 73 sessions. The data gathered across multitreatments design (A-BCD-A-BD-DE) were visually analyzed. The results revealed that all the treatments reduced the occurrences of stereotyped behavior, the significant differences among any interventions were not found, and more importantly, the behavior effects were maintained.

뇌실외 배액술 관리에 대한 이해 및 업무수행 실태

김정숙,윤미선,송소이,이지은,문경선,고지운,임분남,김경희,박경숙 중앙대학교 간호과학연구소 2001 중앙간호논문집 Vol.5 No.2

The objective of this study was to investigate the managing realities of EVD and provide a descriptive survey with the attempt to obtain basic data needed for the standardization of EVD management. For that purpose, 220 questionnaire copies were distributed to those nurses who were working with intensive care units at university hospitals, general hospitals, semi-general hospitals in Seoul, Kyongki-de and local areas. Of 158 returned questionnaire copies, 139 were used for analysis. Data were collected during the term of academic seminars of Neurosurgery Nursing Committee and KICA (Korea Intensive Care Association), ranging from Oct. 26 through 27. 2000. The tools utilized a structured questionnaire form consisting of 21 questions on general characteristics, the level of understanding, the level of work performance, standard management guidelines, etc. The data collected were analysed with frequency, percentage, mean vague, chi -square test using the SPSS 10.0 program Findings were revealed, as follows: For managing the prevention of EVD infection, aseptic technique was found to be of the most importance (48.9%),'the technique should be carried out at an operation room'(92.8%), but it was actually performed at intensive care units (over 51.5%). 'The term of antibiotics use should be less than 7 days' (81.8%), while it was actually executed for less than 7 days (49.5%). 'The proper time of quittance from it should be within less than 7 days'(74.1%) but actual quittance was executed only by 51.1%. 'Set exchange is needed' by 54.0% but actual exchange was implemented merely by 28.1%. 'The subject of management should be nurses' was reflected by 40% but actually 52.0% were carried out. As a result of hating analysed the understanding of the management of EVD by general characteristics, a significant difference was found in the necessity for set exchange by hospitals, in the time of quittance of a catheter, and in ablution items by work experience. The result of hating analysed the work performance levels of EVD management by general characteristics revealed that a significant difference in administration facilities of medicine by the classification of hospitals, especially the use of antibiotics and the quitting time of a catheter by the classification of intensive care units, and in the use of antibiotics and ablution items by the standard management guidelines.

일부 자동차 공장 근로자들의 職業的 特性과 社會 心理的 Stress의 相關性 硏究

김성삼,홍지우,한현정,최희석,권소희,정해경,김삼태,송용선,이기남 한국전통의학연구소 2004 한국전통의학지 Vol.14 No.1

〈Objective〉 This study were carried out to study to assess the relationship between job characteristics, general characteristics, health behaviors, and psychosocial distress and suggested the preventive oriental medicine approaches in occupational health area. 〈Methods〉 We measured job characteristics(job demand, job control, jop insecurity) by The Karasek's Job Content Questionnaire, health behaviors(smoking, drinking, exercise, coffee), general characteristics at work by a self-administered questionnaire. Psycho social distress was measured by PWI(Psychosocial Well-being Index), a 18-item self- administered instrument. 370 data for research were collected through the process of oriental health examination with questionnaire in workplace. Statistical analysis was done by t test, oneway ANOVA, and hierarchical multiple regression analysis with SPSSWIN (version 10.0) 〈Results〉 Among the 152 respondents, the prevalence of high job strain was 27%(41 persons). The Odds ratio of high job strain was 3.11(95 2.12~4.11), and those of passive group and active group were 2.01(95 0.83~2.76) and 1.80(95 0.83~2.76), respectively. Our results supported the association between psychosocial distress and job strain. 〈Conclusion〉 The odds ratio of high strain isolated group(high strain group+low social support) was 3.59(95 2.05 5.13), but the odds ratio of high strain collective group was 1.32(95 0.23 2.41). Social support at work was modifing factor of the association between psycho social distress and job strain. Therefore we reviewed the evidence that economic depression was associated with psycho social distress in this study, and that the strength of the social support was important in maintain health.

정신분열병의 유전적 고위험군에서 인지, 정서 및 사회기능 연구

이경진,위휘,유소영,이애라,송지연,하태현,홍경수,김명선,권준수 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objectives : Vulnerability marker in biological relatives of schizophrenia may be very useful in identifying the propensity for the development of schizophrenia. This study suggests that the relatives at risk for genetically developing schizophrenia have impairments in several domains of cognition, social function and affective function as a vulnerability marker. Methods : A neurocognitive function test, a social function test and an affective function test were administered to fifteen healthy relatives from families with two or more patients with schizophrenia (geneticallly high risk subjects). Fifteen healthy controls matched for age and gender were recruited in this study. Results : Compared to the control subject, the genetic high risk subjects performed significantly more poorly in the selective attention and recall memory of neurocognitive function. The high dsk subjects also had lower scores in independence-performance and independence-competence of the social function test and emotion control of the affective function test. Conclusion : Selective attention, recall memory, independence-performance, independence-competence and emotion control ability may be a valuable marker for genetic study of schizophrenia.

일차배양된 설치류 호흡기 상피세포로부터의 점액소 분비에 대한 수종 약물의 영향

이충재,석정호,이재흔,허강민,박지선,배소현,노삼길,박상철 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

1. PKC activator인 PMA는 일차배양 HTSE세포로부터의 뮤신분비를 0.1μM 농도에서 30%, 1μM 농도에서 80% 가량 증가시켰다. 2. 식물 유래 성분으로, flavonoid의 일종인 TFR은 일치배양 HTSE 세포로부터의 뮤신분비를 10μM 농도에서 50%, 100μM 농도에서 80% 가량 증가시켰다. 3. 양이온성 폴리펩티드인 PLL 및 PLA는 일차 배양 HTSE 세포로부터의 뮤신분비를 0.01 - 10μM 농도에서 용량의존적으로 감소시켰다. 4. 결론적으로, 본 연구에서 얻어진 결과들은 새로운 거담제 및 점액용해제나 단백분해 효소제가 아닌 호흡기 류신의 생성/분비를 조절해 줄 수 있는 신개념의 약물을 개발함에 있어 극히 일부분이나마 단서를 제공하고 있다고 하루 수 있을 것이다. In the present study, we tried to investigate whether phorbol myristate acetate(PMA), trihydroxymethoxy-flavanone rutinoside(TFR) and cationic polypeptides significantly affect mucin release(secretion) from cultured hamster tracheal surface epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hr and chased for 30 min in the presence of each agent to assess the effect on 3H-mucin release. The results were as follows : (1) Both PMA and TFR significantly increased mucin release from cultured HTSE cells ; (2) Cationic polypeptides including po1y-L-lysine(PLL, mw 7,500) and poly-L-arginine(PLA, mw 10,800) significantly inhibited mucin release from cultured HTSE cells, in a dose-dependent manner. This finding suggests us that PMA and TFR be further studied for the possible use as mild expectorants and cationic polypeptides might function as a regulator for hyper-secretion of mucus, both by direct acting on airway mucin-secreting cells, during the treatment of chronic airway diseases.

Antifungal activities of zoysiagrass (Zosia japonica Steud.) chitinases against Rhizoctonia solani and analysis of fungus responsive cis-elements in chitinase genes promoter

Ji-Nam Kang,So-Mi Kang,Hong-Gyu Kang,Hyeon-Jin Sun,Yong-Ik Kwon,Suk-Min Ko,Hyo-Yeon Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Zoysiagrass are damaged by fungi diseases such as large patch, dollar spot, pythium blight and brown patch. Large patch is one of the major diseases caused by Rhizoctonia solani AG2-2 on zoysiagrass fields e.g. golf courses. Plant chitinases have been known PR (Pathogen related)-protein. In this study, we isolated two chitinase genes (Zjchi1 and Zjchi2) from zoysiagrass. Antifungal activity analysis revealed that Zjchi2 protein inhibited mycelium extension of fungi. A further study, we cloned 5` upstream region from two chitinase genes for investigating transcription regulatory mechanism that inducing of two chitinase genes dependent R. solani. -818 bp and -799 of upstream region from Zjchi1 and Zjchi2 successfully isolated using in vitro LA (Long and Accurate) PCR system. And then, we generated promoter-GUS reporter constructs with deletion construct based on W-boxes. Constructs were introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation for stable expression of GUS reporter gene.