Cytological analysis of pregnancy-associated plasma protein-A expression in porcine neonatal testis

Ji-youn Kim,Keon Bong Oh,Sung June Byun,Sun-A Ock,Hwi-Cheul Lee,황성수,SangHyun Park,Wootae Ha,Jae-Seok Woo,Hyuk Song 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3

The identification of biomarkers of a living tissues is essentially required to understand specific functions of the cells. In previous study, we reported IGFBP 3 as one of the putative biomarkers, by showing specific expression at porcine spermatogonial stem cells (SSCs) of early stage of porcine testis. In this study, we analyzed the expression of seven members of IGFBP family (IGFBPs) in SSCs and histological expression pattern of pregnancy-associated plasma protein-A (PAPP-A), which plays a role on the growth promoting enzyme by cleavage of IGFBPs in testis of 5 days old pig. RT-PCR analysis showed that IGFBP 1, 2, 3, 4, and 6 were expressed at high level specifically in porcine SSCs compared with whole testis. We performed immunohisotochemical staining of testis sections with PAPP-A and protein gene product 9.5 (PGP9.5) which are the known biomarkers for SSCs. We were not able to find co-expression of PAPP-A and PGP9.5; PAPP-A was expressed only in Sertoli cells and PGP9.5 expression was confirmed in spermatogonium. Additionally, we were able to confirm the GATA4 expression in Sertoli and Leydig cells as a regulator of Sertoli cell function was not detected PGP9.5 expressing cells, indicating indirect evidence of that cytolocalization of PAPP-A expression is limited in Sertoli cells. These results suggested that the PAPP-A expressed in Sertoli cells may play role on regulation of development and differentiation of testicular cells through the IGF axis in neonatal porcine testis.

중학교 과학, 고등학교 공통과학, 및 화학 Ⅰ,Ⅱ 교과서의 물질 반응에 대한 연계성 연구

강지아,노봉오 조선대학교 사범대학 부설 교과교육연구소 2000 敎科敎育硏究 Vol.3 No.1

The effectiveness of continuity of material changes in middle school and high school textbooks was investigated on the basis of the sixth curriculum. For rhis purpose, five types of classification rules are set up for analysis of the concept: initial, overlap, development, gap, and wide gap. The results indicated that decelopment and gap categories were 50% and 30%, repaectively, from middle school science textbooks to high school common science textbooks, and that overlap and development were 60% in the middle school science textbooks to chemistry Ⅰ textbooks in the high school. Well developed sequence of terms are basic law of atoms and molecules, molecular weight, and ion and chemical formulas. The areas of a big gap from middle school science textbooks to high school chemictryⅠand Ⅱ textbooks are atom energy level and electrode potentials. The chapter on chemical kinetics in the high school common science textbooks is a new concept and a gap from middle school science textbooks. Therefore this chapter needs to be changed to treat either acid-base equilibrium, solutions, or atom, molecules and ions.

A Driving Method of Pixel Circuit Using a-IGZO TFT for Suppression of Threshold Voltage Shift in AMLED Displays

Woo-Sul Shin,Hyun-A Ahn,Jun-Seok Na,Seong-Kwan Hong,Oh-Kyong Kwon,Ji-Hun Lee,Jae-Gwang Um,Jin Jang,Sung-Hwan Kim,Jeong-Soo Lee IEEE 2017 IEEE electron device letters Vol.38 No.6

<P>A driving method of pixel circuit using amorphous indium gallium zinc oxide (a-IGZO) thin-film transistor (TFT) is proposed to improve the image quality of active matrix light-emitting diode displays. The proposed pixel circuit employs a diode-connected structure to compensate for variation in threshold voltage (V-th) of the a-IGZO TFT. In addition, the proposed driving method adopts negative bias annealing to suppress the V-th shift. The annealing time is optimized based on the experimental observation of the minimum V-th shift. After a stress time of 30 000 s, the measurement results showthat the (Vth) shift is reducedby 29.6%, using an optimized annealing time of 5% of one frame time. In addition, the maximum deviation in the emission current using the proposed driving method wasmeasured to be less than 4.32% after a stress time of 30 000 s.</P>

Upregulation of NAD(P)H:quinone oxidoreductase by radiation potentiates the effect of bioreductive beta-lapachone on cancer cells.

Choi, Eun K,Terai, Kaoru,Ji, In-Mi,Kook, Yeon H,Park, Kyung H,Oh, Eun T,Griffin, Robert J,Lim, Byung U,Kim, Jin-Seok,Lee, Doo S,Boothman, David A,Loren, Melissa,Song, Chang W,Park, Heon Joo Stockton Press 2007 Neoplasia Vol.9 No.8

<P>We found that beta-lapachone (beta-lap), a novel bioreductive drug, caused rapid apoptosis and clonogenic cell death in A549 human lung epithelial cancer cells in vitro in a dose-dependent manner. The clonogenic cell death caused by beta-lap could be significantly inhibited by dicoumarol, an inhibitor of NAD(P)H:quinone oxido-reductase (NQO1), and also by siRNA for NQO1, demonstrating that NQO1-induced bioreduction of beta-lap is an essential step in beta-lap-induced cell death. Irradiation of A549 cells with 4 Gy caused a long-lasting upregulation of NQO1, thereby increasing NQO1-mediated beta-lap-induced cell deaths. Although the direct cause of beta-lap-induced apoptosis is not yet clear, beta-lap treatment reduced the expression of p53 and NF-kappaB, whereas it increased cytochrome C release, caspase-3 activity, and gammaH2AX foci formation. Importantly, beta-lap treatment immediately after irradiation enhanced radiation-induced cell death, indicating that beta-lap sensitizes cancer cells to radiation, in addition to directly killing some of the cells. The growth of A549 tumors induced in immunocompromised mice could be markedly suppressed by local radiation therapy when followed by beta-lap treatment. This is the first study to demonstrate that combined radiotherapy and beta-lap treatment can have a significant effect on human tumor xenografts.</P>

11β-수산화효소 결핍에 의한 선천성 부신증식증 1예

류옥현,류혜진,박수연,권순범,박상수,김희영,이계원,서지아,오정헌,김신곤,김난희,최경묵,백세현,최동섭 대한내분비학회 2004 Endocrinology and metabolism Vol.19 No.1

저자들은 국내에서 처음으로 음핵 비대와 지속적인 질출혈을 주소로 내원한 19세 여성에서 고혈압이 동반되지 않으면서 11 -수산화효소 결핍으로 인한 선천성부신증식증을 경험하였기에 문헌 고찰과 함께 이를 보고하는 바이다. Congenital adrenal hyperplasia refers to a group of autosomal recessive disorders that is defective in the synthesis of cortisol. The enzymes most often affected are 21-hydroxylase and 11 - hydroxylase. The low levels of cortisol stimulate the pituitary gland to release ACTH. Chronic elevation of the ACTH level causes bilateral adrenal hyperplasia and a secondary increase in androgen formation. We examined a 19 year-old woman presented with clitoral hypertrophy and vaginal spotting. The subjects basal level of serum cortisol was low, but the serum levels of ACTH, 17a-hydroxyprogesterone, deoxy-corticosterone were elevated. The urinary excretions of 17-ketosteroids and 17-hydroxycorticosteroids were also increased. The karyotyping study and transrectal ultrasonography showed normal findings. The patient underwent clitoris reduction surgery and received hydrocortisone. To the best of our knowledge, this is the first case of 11 -hydroxylase deficiency in Korea (J Kor Soc Endocrinol 19:58∼63, 2004).

Anti-Asthmatic Activities of an Ethanol Extract of Aster yomena in an Ovalbumin-Induced Murine Asthma Model

Sim, Ji Hyun,Lee, Hyun Seung,Lee, Sunkyung,Park, Dae Eun,Oh, Keunhee,Hwang, Kyung-A,Kang, Hye-Ryun,Ye, Sang-Kyu,Kim, Hang-Rae The Korean Society of Food Science and Nutrition 2014 Journal of medicinal food Vol.17 No.5

Aster yomena is used in traditional remedies to treat cough, asthma and insect bites; however, its therapeutic mechanism is not completely understood. To elucidate the anti-asthmatic effect of A. yomena, we investigated the anti-asthmatic characteristics of an alcohol extract of A. yomena in an ovalbumin (OVA)-induced murine asthma model. In this study, we showed that A. yomena extract inhibited the overall pathophysiological features of asthma by suppressing Th2 responses and enzymes associated with the production of inflammatory mediators. This suppression resulted in decreased Th2 type cytokines and eosinophils in the bronchoalveolar lavage fluid and OVA-specific IgE in serum. Additionally, A. yomena extract significantly decreased airway hyperresponsiveness and abrogated the histopathological changes in the lungs, which reached normal levels in the OVA-challenged mice treated with A. yomena extract. These findings suggest that A. yomena could be a promising natural agent for treating bronchial asthma in humans.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

유독와편모조류 Alexandrium tamarense와 Alexandrium catenella의 성장에 미치는 조도의 영향

권형규 ( Hyeong Kyu Kwon ),박지아 ( Ji A Park ),양한섭 ( Han Soeb Yang ),오석진 ( Seok Jin Oh ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.12

The effects of irradiance on the growth of toxic dinoflagellates Alexandrium tamarense (Masan Bay strain) and Alexandrium catenella (Jinhae Bay strain) were investigated in the laboratory. At 15℃ and 30 psu for A. tamarense and 25℃ and 30 psu for A. catenella, the irradiance-growth curve showed the maximum growth rate (μmax) of 0.31 day-1 with half-saturation photon flux density (PFD) (KI) of 44.53 μmol m-2 s-1, and a compensation PFD (Ic) was 20.67 μmol m-2 s-1 for A. tamarense, and μmax of 0.38 day-1 with KI of 59.53 μmol m-2 s-1, and Ic was 40.80 μmol m-2 s-1 for A. catenella. The Ic equated to a depth of 8~9 m from March to June for A. tamarense and 6~7 m from March to June for A. catenella. These responses suggested that irradiance at the depth near the middle layer in Masan Bay would provide favorable conditions for two species.

대학생의 취업스트레스와 자아존중감, 자아효능감, 외모관리행동의 관계

김보라,김유진,김지희,노혜진,오승희,홍지혜,황윤희,박선아 水原大學校 2016 論文集 Vol.30 No.-

Purpose: Many university students are under a lot of job-seeking stress because the employment rate is low. This study sought to determine the relationship between job-seeking stress, self esteem, self efficacy and appearance management behavior amongst university students focusing on nursing. Methods: This descriptive correlational study evaluated the relationships between job-seeking stress, self esteem, self efficacy and appearance management behavior of 340 university students was conducted between September 1 and October 5, 2015. Results: The correlational analysis between job-seeking stress, self esteem, self efficacy and appearance management behavior revealed a negative correlation between job-seeking stress, self esteem and self efficacy and a positive correlation between job-seeking stress and appearance management behavior. In addition, age also correlated with self esteem, self efficacy and appearance management behavior. Conclusion: Stress correlated with self esteem and self efficacy and appearance management behavior. Therefore job-seeking stress may be reduced through not only self esteem and self efficacy enhancement programs but also management according to age.

Bioassay-guided isolation and identification of anti-obesity phytochemicals from fruits of Amomum tsao-ko

Hong Seong Su,Choi Chun Whan,Lee Ji Eun,Jung Yeon Woo,Lee Jung A,Jeong Wonsik,Choi Yun-Hyeok,Cha Hanna,Ahn Eun-Kyung,Oh Joa Sub 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.1

Amomum tsao-ko (Zingiberaceae), an important traditional medicinal herb, possesses many biological activities, including anti-inflammatory effects. Though the anti-obesity properties of the crude ethanol extract of A. tsao-ko fruits have been reported, the anti-adipogenic properties of its phytochemical constituents have not been reported. Therefore, in the present study, we isolated the active constituents of A. tsao-ko and investigated their anti-adipogenic effects. The bioassay-guided isolation of the phytochemicals from the ethanol extract of A. tsao-ko fruits identified four bioactive compounds, comprising one fatty acid (1), one sesquiterpene alcohol (2), and two phenolic compounds (3 and 4). Their structures were elucidated by a combination of 1D and/or 2D nuclear magnetic resonance and mass spectrometry. The anti-adipogenic activities of the four compounds evaluated by Oil Red O staining in 3T3-L1 cells revealed that the treatment with the isolated compounds 1 and 3 reduced the lipid accumulation in 3T3-L1 adipocytes more strongly than the compounds 2 and 4, in a dose-dependent manner.