한국산 초파리집단의 발생율에 대한 자기장의 영향

정승문,박은규 圓光大學校 基礎自然科學硏究所 2000 基礎科學硏究誌 Vol.18 No.1

거제도에서 채집한 야생형 초파리와 성연관 돌연변이체 사이에서의 성적선택에 따른 자기장의 영향을 조사하기 위하여 본 연구를 수행했다. 본 실험에서의 초파리는 4개의 다른 자기장의 힘의 영향하에서 사육되었다. 즉 지구자기장, 탈지구자기장, 지구자기장 2배의 자기장과 지구장기장 8배의 자기장을 설정하고 이들 제어환경하에서 상기 재료 초파리들의 수정란에서 우화하기까지의 발생율을 조사하여 그 결과를 분석하였다. 전체평균 발생율은 지구자기장내의 초파리 집단, 탈지구자기장내의 초파리 집단, 지구자기장 2배의 초파리 집단, 지구자기장내 8배의 초파리 집단에서 각각 20.35%, 24.55%, 25.27%, 29.83%로 지구자기장내의 초파리 집단 전체평균이 Helmholtzcoils 장치를 이용해 조절된 다른 3개의 초파리 집단에서 보다 상대적으로 낮은 발생율을 보여 주었다. 상이한 자기장내에서의 계수일자에 관한 발생빈도를 보면, 계수 첫째날부터 셋째날까지의 빈도가 지구자기장내 초파리집단에서 약 63%가, 탈지구자기장 집단에서는 약70%, 지구자기장 2배의 집단에서는 65%를, 그리고 지구자기장 8배의 초파리 집단에서는 전체 평균 발생빈도의 74%를 넘는 것으로 관찰되었다. This study was carried out to elucidate influence of magnetic field on sexual selection between sex-linked mutant and the wild type of Drosophila melanogaster collected at keojedo. For the present experiment using population cages, each fly of four cages reared under the influence of four different strengths of magnetic field, that is, the ambient, the without, the double and the eight-fold magnetic field artificially generated by Helmholtz coils. Also the study was analysed emergence frequencies of progeny on counting days due to eggs laying by mated females on a complete food. The over-all mean emergence rates of population of 25 generations reared under the four different magnetic fields, the ambient, the without, the double and the eight-fold, were 20.35%, 24.55%, 25.27% and 29.83%, respectively. The fly population raised under the ambient magnetic field showed a relatively lower emergence rate than any of those raised under the other three magnetic fields. Emergence frequencies of progeny on counting days from 1st to 3rd day under different magnetic fields were above 63%, 70%, 65% and 74%,respectively.

IL-β의 인슐린 분비 자극효과와 그 과정에 관여하는 인자들

정인경,오승훈,강동묵,정재훈,민용기,이명식,이문규,김광원 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.4

연구배경: IL­1β는 용량과 노출시간에 따라 인슐린분비에 대해 상반되는 효과를 가지고 있다. IL­1β의 인슐린 분비 억제효과는 제1혁명 당뇨병의 자가면역 기전과 관련되어 잘 알려져 있으나 인슐린 자극효과에 대해서는 아직 명백히 밝혀지지 않았다. 이에 저자 등은 IL­1β의 다양한 농도에 따라 백서의 췌도세포에서 인슐린 분비에 미치는 영향을 살펴보고, 그 기전으로 인슐린 생합성, iNOS의 발현, 칼슘통로의 활성도 변화여부를 알아보고자 하였다. 방법:200∼300g인 수컷 Sprague­Dawley 백서의 췌도를 변형된 Lacy&Kostianovsky's 방법으로 분리한 후 IL­1β의 다양한 농도(0, 0.5, 5, 50, 500pmol/L)에 2, 6, 24시간 노출시켜 췌도세포의 형태, 생존능을 관찰하고 인슐린 분비능 및 췌도세포내 인슐린 함량을 측정하였으며, 전전구 인슐린 mRNA발현, iNOS mRNA발현을 RT­PCR을 통해 확인하였고, 세포의 칼슘 통로 활성도 변화 여부를 측정하였다. 결과:1) IL­1β에 노출되 췌도의 생존능:2시간 노출시는 대조군과 차이가 없었으나, 6시간 고농도군과 24시간 모든 군에서 생존능이 감소되었다. 2)인슐린 분비능은 IL­1β를 2시간 5poml/L이상의 고농도와 6시간 0.5pmol/L 저농도 처리시 대조군에 비해 의미있게 증가하였으나, 6시간과 24시간 5poml/L이상의 고농도에서는 의미있게 인슐린의 분비가 억제 되었다. 3)췌도내 인슐린양의 변화는 IL­1β의 시간과 농도에 따라 배지내의 인슐린 변화와 비슷한 경향을 보였으나 통계학적으로 의미있는 차이는 없었다. 4)전전구인슐린의 mRNA발현은 2시간 50pmol/L이상 고농도의 IL­1β에서 의미있게 증가하였고, 6시간 처리군에서는 의미있는 차이는 없었으나, 24시간에서 IL­1β의 용량의존적으로 억제되었다. 5)iNOS mRNA는 IL­1β 처리 후 2시간부터 발현되기 시작하여, 6시간에 최고에 달한 후 24시간에는 점차 감소하였다. IL­1β의 처리시간과 무관하게 5poml/L이상의 고농도에서 용량에 따라 발현이 증가하였다. 6)칼슘통로 활성도는 IL­1β의 농도나 시간에 따라 유의한 차이가 없었다. 결론: IL­1β는 단시간 고용량이나 장기간 저용량에서 인슐린 분비와 생합성을 증가시키며, 이런 효과는 iNOS나 칼슘 통로 활성도 변화와는 무관한 것으로 생각된다. Background : The inhibitory effort of IL-1β on the insulin secretion has been validated in pathogenesis of type 1 diabetes, but complex results about the stimulatory effect of IL-1β have been reported. The aims of this study are to clarify the effects of IL-1βon insulin secretion of pancreatic islets and to investigate the mechanisms in terms of preproinsulin synthesis, inducible NOS expression, and calcium channel activity. Method : Islets were isolated from male Sprague-Dawley (SD) rat by modified Lacy-Kostianovsky's method. After islets were treated with different concentrations (0, 0.5, 5, 50, 500 pmol/L) and exposure time (2, 6, 24 hours) of IL-1β, morphology, viability, static stimulation of insulin to glucose, insulin content, preproinsulin mRNA expression, iNOS mRNA expression and calcium channel activity were measured. Results : 1) Viability o islets was reduced in high concentrations of long term exposure of IL-1β. 2) Insulin secretion was stimulated in islets treated with 5, 50, and 500 pmol/L of IL-1β for 2 hours. 3) Insulin content was not significantly different regardless of concentration and exposure time of IL-1β. 4) Preproinsulin mRNA expression increased in islets treated with 50, 500 pmol/L of IL-1β for 2 hours. After 24 hours, it decreased in dose dependent manner. 5) iNOS mRNA expression was detectable after 2 hours in the presence of IL-1β, peaks at 6 hour and decreased after 24 hours. It was increased above 5 pmol/L of IL-1β in dose dependent manner. 6) Activities of the voltage-dependent Ca^2+ channels were not different among groups. Conclusion : IL-1β plays a positive role in terms of insulin secretion and insulin synthesis in high concentration of short term or low concentration of long term. These effects of IL-1β might be neither dependent of iNOS pathway nor Ca^2+ channel activity (J Kor Diabetes Asso 431~443, 2000).

탈지구자기장이 초파리 집단의 선택불리에 미치는 영향

정승문,박은규 圓光大學校 基礎自然科學硏究所 2001 基礎科學硏究誌 Vol.19 No.1

본 연구에서는 전북 김제시 청하의 자연집단 야생형 초파리와 성연관 골연변이형 호아색체-백색안 초파리를 Helmholtz coils 장치를 사용해서 유도한 탈지구자기장내 초파리 집단과 비교집단인 지구자기장내 초파리 집단으로 사육해서 발생된 성적 선택빈도를 조사하여, 선택불리에 관한 다음과 같은 결과를 얻었다. 야생형 유전자를 운반한 염색체의 선태빈도는 25세대까지 전체 평균빈도가 위의 두 집단에서 각각 0.7284,0.7360으로 관찰되었다. 이 결과는 탈지구자기장내 초파리 집단에서 더 강한 선택불리를 보여 주었다. 성연과 돌연변이형 yω 유전자를 운반한 염색체의 선택빈도는 위의 각각의 조절된 자기장내의 초파리 집단에서 각각0.0256, 0.0191로 관찰되었다. 이 결과는 탈지구자기장내 초파리 집단보다 지구자기장내 초파리 집단에서 더 강한 선택불리 행동을 보여 주었다. 초파리 집단의 전체 평균 발생율은 위의 초파리 집단에서 각각 32.10%, 67.90%로 관찰 되었다. 이 결과는 지구자기장내 초파리 집단의 발생율이 상대적으로 높은 수치로 조사되었다. Duncan's multiple range test와 LSD 값으로 유의 차이를 검정하였던 바 야생형 그룹과 다른 3개의 돌연변이형 그룹 사이에서 고도의 유의 차이가 있음이 밝혀졌다. To elucidate the genetic behavior of selective disadvantage among sex-linked mutants of Drosophila melanogaster vred and kept by the genetic study laboratory room, Wonkwang University and wild type collected at Chongha, Kimje city, Chollabuk-do, and reared under the in fluence of two different strength of magnetic field, this study i.e., the without magnetic field generated by Helmholtz coils and the ambient magnetic field, this study was carried out at the above indicated laboratory room. The mean selective frequencies of wild type gene-carrying chromosomes up to twenty-fifth generation, affected by two different magnetic fields, showed 0.7284 and 0.7360 with the magnetic field strength as mentioned other before. The data explains that Drosophila melanogaster population reared in the without magnetic field showed stronger selective disadvantage. The selective frequencies of the individual of yω gene-bearing chromosomes showed 0.0256 and 0.0191 by the same order of magnetic field strength indicate above. It was shown that the fly population reared at the ambient magnetic field cleared the relatively stronger selective disadvantage. The over-all mean emergence rates of Drosophila melanogaster populations reared in controlled magnetic fields indicated above showed 32.10% and 67.90% respectively higher emergence rate than the without magnetic field population. Through Duncan's multiple range test and LSD value, the highly significant differences between the wild type and anyone of the other mutants were calculated.

한국 초파리 집단의 선택불리에 관한 지구 자기장이 소거된 자기장 영향

정승문,최오옥,박은규 圓光大學校 1996 論文集 Vol.32 No.2

본 연구에서는 한국 남해안 거제도의 자연집단의 야생형 초파리와 성연관 돌연변이형 황색체-백색안 초파리를 Helmholtz coils 장치를 사용해서 유도한 지구 자기장이 소거된 초파리집단과 비교집단인 지구 자기장내의 초파리 집단으로 사육해서 발생된 성적 선택빈도를 조사하여, 선택불리에 관한 다음과 같은 결과를 얻었다. 야생형 유전자를 운반한 염색체의 선택빈도는 20세대까지 전체 평균 빈도가 위의 2집단에서 각각 0.7212, 0.7180으로 관찰되었다. 이 결과는 지구 자기장이 소거된 집단에서 더 약한 선택분리 행동을 보여주었다. 성연관 돌연변이형 yw 유전자를 운반한 염색체의 선택빈도는 위의 각각의 조절된 자기장내의 초파리 집단에서 각각 0.0306, 0.0372로 관찰되었다. 이 결과는 자기장이 소거된 초파리 집단 보다 지구 자기장 내의 초파리 집단에서 더 약한 선택불리 행동을 보여 주었다. 초파리 집단의 전체 평균 발생율은 위의 초파리 집단에서 각각 53.40%, 40.60%로 관찰되었다. 이 결과는 지구 자기장내의 초파리 집단의 발생율이 상대적으로 낮은 수치로 조사되었다. Duncan's multiple range test와 LSD값으로 유의 차이를 검정하였던 바 야생형 그룹과 다른 3개의 돌연변이형 그룹 사이에서 고도의 유의차이가 있음이 밝혀졌다. To elucidate the genetic behavior of selective disadvantage among sex-linked mutants of Drosophila melanogaster bred and kept by the genetic study laboratory room, Wonkwang University and the wild type collected at Keojaedo island, and reared under the influence of two different strength of magnetic fields, i.e. the without magnetic field generated by Helmholtz coils and the ambient magnetic field, this study was carried out at the above indicated laboratory room. The mean selective frequencies of wild type gene-carrying chromosomes up to twentieth generation, affected by two different mafnetic fields, showed 0.7212 and 0.7180 with the magnetic field strength as mentioned other before. The data explains that Drosophila melanogaster population reared in the without magnetic field showed lower selective disadvantage. The selective frequencies of the individual of yw gene-bearing chromosomes showed 0.0306 and 0.0372 by the same order of magnetic field strength indicated above. It was shown that the fly population reared at the ambient magnetic field cleared the relatively lawer selective disadvantage. The over-all mean emergence rates of Drosophila melanogaster populations reared in controlled magnetic fields indicated above showed 53.40% and 46.60% respectively. Referring to these data, the fly population raised in the ambient magnetic field showed a relatively lower emergence rate than the without magnetic field population. Through Duncan's multiple range test and LSD value, the highly significant differences between the wild type and anyone of the other three mutants were calculated.

흰쥐에서 심정지후 Pentoxifylline이 뇌의 허혈-재관류 손상에 미치는 효과

정시경,김영민,오동렬,최경호,박승현,이운정,박규남,유은영,이원재,김세경 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Two major events occurring in the cerebral hemodynamics after successful resuscitation from cardiac arrest are reactive hyperemia and postischemic hypoperfusion. In this study, we examined the effect of Pentoxifylline(PTX) on the rat brain following cardiac arrest. Methods: Fourteen rats were anesthetized and artificially ventilated. Cardiac arrest was produced by chest compression and clamping of tracheal tube for 3 minutes in ketamine anesthetized rats. Circulation was restored by standard cardiopulmonary resuscitation methods. In 7 rats, PTX 10mg/kg was infused at 10min after cardiac arrest(PTX group). In the other 7 rats, same amount of normal saline was infused(control group). Results: In both groups, hemodynamic variables, neurologic deficit(ND) score and histopathologic findings of hippocampal CA1 neurons were observed. Hemodynamic variables and ND score were not significantly different between two groups. Delayed ischemic neurons of hippocampal CA1 were decreased in PTX group(2.2±2.4%) compared with control group(9.1±1.2%). Conclusions: We conclude that PTX prevented development of delayed ischemic neurons in hippocampal CA1 after cardiac arrest. PTX may be useful in emergency situations following cardiac arrest.

Helm-Holtz coil裝置 초파리集團의 選擇不利에 관한 遺傳學的 硏究

鄭勝文,朴泰永,朴殷圭 圓光大學校 基礎自然科學硏究所 1990 基礎科學硏究誌 Vol.9 No.2

In order ot elucidate the genetic behavior of sexually selective disadvantage between visible mutants and wild type of Drosophila melanogaster in the Helm-Holtz coil apparatus without the ambient magnetic filed, this study was carried out From the result of the present experiment, the frequencies of all the flies between in the apparatus and .in the ambient field showed that the mutants decreased and the wild type increased according to the process of the generations. The whole average frequencies of the + gene-bearing chromosomes on the selective advantage through initial to twenty five generations in the apparatus and in the field were 72.84%, and 73.60%, respectively. It was observed that the sexually selective disadvantage of the + gene of wild type in the apparatus were higher than 'hat in the field. Each frequency on the sexually selective advantage rates of the yw gene-bearing chromosomes showed as 2.56% in the apparatus and as 1.91% in the field. Thus the selective disadvantage of the farmer was lower thatn that of the latter. The average frequencies of the w and the y gene-bearing chromosomes in the selective advantage rates were nearly the same under both conditions of the apparatus and the field showing 0.07%, 0.08% and 0.03%, 0.03%, respectively. The percentage of progeny due to eggs Lying by mated females on a complete food in the apparatus and in the field was found that each frequency in the whole average frequencies revealed 35.23% in the apparatus and 64.77% in the field. This suggested that the frequencies of all the flies in the apparatus decreased to nearly half of that of them in the field. The average frequency of each gene through initial to twenty five generations in the apparatus and in the field was calculated to be 72.84% and 73.60% in + gene. 2.56% and 1.91% in yw gene, 0.07% and 0.08% in w gene, 0.03% and 0.03% in y gene, respectively. From these data, the yellow bodied mutant was the highest in selective disadvantage and weakened in order of wild, yellow-white and white eyed mutant And summarized are that, as to the gene frequencies of wild, yellow-white, white, and yellow of Drosophila melanogaster, strength and weakness of selective disadvantage was varied due to X-linked genes.

주의력결핍 과잉행동장애 아동의 연령에 따른 특성비교

정영철,이종범,박형배,정성덕,성형모,사공정규 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.1

Objectives : This study is to verify the developmental changes of ADHD symptoms and functions with aging from 7 to 12 year-old. Method : The subjects of this study were 120 children(108 boys and 12 girls) who were diagnosed as ADHD by DSM - IV at department of neuropsychiatry of Yeungnam University Medical Center. Multidementional tests(K-ABC, ADDES-HV, CAP, CPT, KPl-C, WCST) were carried out to evaluate the change of ADHD symptoms and functions. Results : In K-ABC, children with ADHD showed statistically significant increased "Reading and Understanding" ability(p<.001) with age. In ADDES-HV, They showed decreased inattention (p<.05) and hyperactivity(p<.05) but, not showed significant change of impulsivity symptom with age. In CAP, They showed decreased hyperactivity(p<.001) and inattention (p<.05) and lower total score(p<.05) with age. their result were similar with ADDES-HV. In CPT, They showed decreased hit reaction time(p<.001) and hit reaction time standard errors(p<.01) and variability of standard errors(p<.05) with age. In KPI-C, They showed decreased anxiety(p<.01), depression(p<.001), somatization(p<.05), delinquency(p<.05) and hyperactivity(p<.01) with age. Social relationship(P<.05) was improved, as well as decreased psychoticism(p<.05) and autism (p<.001). In WCST, They showed increased completed categories(p<.001), total corrects(p<.001) and fewer total errors (p<.001) with age. Conclusion : Above findings indicate that symptoms of inattention and hyperactivity in school-aged ADHD children tend to decline with age, but age was less associated with decline in impulsivity symptom . In additions, Their executive functions were improved notably and they appeared stability of emotion, close social relationship and decreased delinquency.

초임계 유체 CO₂를 이용한 강황에서의 Curcumin 추출의 최적화

정승현,장규섭,김영종 한국산업식품공학회 2004 산업 식품공학 Vol.8 No.1

강황의 색소성분 curcuminoid의 주성분인 curcumin은 많은 생리활성이 보고 되고 있으나, 현재 유기 용매를 이용한 추출에 의존하고 있어 안전성이 문제시 된다. 따라서 본 연구는 유기용매에 대한 위험성이 적은 초임계 유체 추출을 통한 강황 중 curcumin 추출의 최적화를 수행하였다. curcumin 추출의 최적 조건을 도출하기 위하여 중심합성법(central composite design)으로 설계된 실험방법으로 반응표면 분석을 실시하였다. 이때 독립변수를 추출온도(X₁), 보조용매유속(X₂), 추출압력(X₃)로 설정하고 curcumin 함량을 종속변수(Y)로 하여 Y=-8.581270+0.220770X₁+1.176731X₂+0.036873X₃+-0.0026816X₁²-0.0000825X₃X₂-0.000096554X₃² 와 같은 회귀식을 도출하였다. 반응표면 분석결과 curcumin 추출량의 최고점은 온도 40.31℃, 압력 231.59bar, 보조용매유속 3.07 ml/min 조건이었고, 이때 curcumin 추출량은 1.9228%였다. Curcumin, the major yellow-colord pigment in turmeric(Curcuma long L.), was extraced by using supercritical carbon dioxide. Opimum extraction conditions were determined. Overall experiments were plaaned by central composite design and result were analyzaed by response surface methodology to find efffect of three independent variables, temperature(X₁), co-solvent flow rate(X₂) and pressire(X₃) on the yield of curcumin extrat(Y). Regression model optimiaed by response surface analusis was as follows Y=-8.581270 + 0.220770X₁+1.176731X₂+0.03683X₃+ -0.0026816X¹₂-0.013010X₂X₁-0.103353X₂²+0.000198X₃X₁-0.0000825X₃X₂-0.000096554X32. Optimum temprature, pressure and so-solvent flow rate for exracting curcumin from turmeric were 40.31℃,3.07㎖/min and 231.59bar, respectively and statistical maximum yield of curcumm was 1.922%.

平生體育에 있어서 老化現象의 生理 및 心理社會學的 理論에 관한 考察

鄭升溶,李揆文 忠北大學校 平生體育硏究所 1985 平生體育硏究所 論文集 Vol.1 No.-

The purpose of this study is to researchphysiologieal and ?? aging and the general struetural and functional changes that occur in adult ??. The aging process is irreverisible. The changer of organs and ?? at different rates. Rates of physiological and psychosocial changes are set by the inter-acting and environmental forces. The six physiological theories presented here attempt to explain the age changes at the cellulan and organism levels. Cerain theories accent the role of geneties, others stress the effects of environment as cental to prolonging length of life. The free radical theory proposes that chemical substances are produced at the cellular level and alter the DNA structure within the cell nucleus. The wear-and-tear theory is based upon the promise that the body ?? wears out, with an inevitable slowing of the metabolic processes until the human machine ??timately stops from exhaustion. The cross-linking (collagen) theory bases its premises upon a build-up of collagen, which becomes stiffer with age. Collagen appears to replace water in bones and also deposits itself in an altered form within numerous body structures, cross-linking with the DNA strands so that they no longer function normally. The autoimmune theory asumes that aging results from a break down of the body's immune system. This theory postulates that age-connected diseases such diabeles, arteritis, arthritis, and cancer result from deviate antkbodies produced by aging cells. According to the genetic mutation theory, body cells undergo form and quality changes that hasten the aging process. Cells are altered so that they are unable to mannfacture essential enzymes necessary for maintaining life. The cellular error theory suggests that errors occur in the formation of a protein or enzyme. Such error produce ?? compounds, which presumably accumulate rapidly, and progressively lead to age changes and death. The activity, dis-engagement, and continuity behavioral theories of aging involve a psychosocial and health fitness perspective. Essentially, the activity theory asserts a positive relationship between successful aging and the individuals level of mental, physical, and social involvement. The disengagement theories regard the gradual with drawal from societal activitles as normal physiological and psychological behavior as one grows older. The continuity theory holds that behavior in old age is an extension of one's previous life style, and one's early habit, preference, and dispositions continue into senior maturity. The several patterns of personality discussed illustrate both well-adjusted and integrated types and the disintegrated sort found among sell-haters.

단계적 온도 하강법을 이용한 췌도세포 냉동보존법

정인경,오승훈,김병준,양태영,이병완,하창영,노정현,정재훈,민용기,이명식,이문규,김광원 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.1

연구배경:최근 당뇨병의 새로운 치료법으로 시도되고 있는 췌도이식은 충분한 췌도수의 확보와 췌도생존율을 높이기 위한 면역억제제 사용이 제한점이 되고 있다. 본 연구의 목적은 이식 전 충분한 췌도 수의 확보를 위해 분리한 췌도를 냉동보존하는 방법을 확립하고 냉동보존한 백서 췌도세포의 시험관내 그리고 생체내 기능을 조사하였다. 방법:분리한 백서의 췌장소도를 48시간 배양한 후 한 시험관당 췌도세포 1000개씩 나누었다. 냉동보존은 6개의 시험관에 DMSO를 첨가한 후 초 냉각(supercooling), 핵화(nucleation)단계를 거친 후 99% isopropanol과 액체질소가 들어있는 dewer를 이용하여-0.25℃/분의 냉각속도로 -40℃까지 단계적으로 얼린후-70℃ 액체질소 탱크에 보관했다. 해동은 냉동시킨 vial들을 액체 질소 태으에서 꺼내 37℃ 항온조에 담가 급격히 해동시킨 후, 원심분리하여 상층액을 제거하고 각 vial에 0.75M sucrose 용액을 가한 후, 10% fetal calf serum이 함유된 RPMI 1640 media에서 배양하였다 각각 6개의 시험관에서 해동한 췌도들을 광학현미경 및 형광현미경하에서 췌도의 모양과 생존율에 대해 조사하고 인슐린 정적반응을 알아보았다. 또한 분리한 췌도를 냉동보존하지 않고 이식한 경우를 대조군(6마리)과 생체내 기능을 비교하였다. 결과:① 냉동보존후 획득한 췌도의 수와 생존율 해동후 획득한 췌도의 수는 해동시킨 당일날이 902±21, 24시간 배양 후에는 857±16, 72시간 후에는 817±18개로 점차 감소되었다. AO/PI 염색상 각 췌도의 생존율은 냉동 전을 100으로 하였을 때 해동당일, 24시간 후, 72시간 후가 각각 60±5, 80±5, 90±5%로 해동후 3일간 배양하였을 때 냉동전의 수준으로 회복하였다. ② 냉동보존후 췌도의 포도당에 대한 정적 인슐린 분비능:냉동직후 감소된 경향을 보였으나 해동후 3일간 배양한 췌도의 인슐린 분비는 냉동전과 통계적으로 의미있는 차이가 없이 냉동보존 전의 수준으로 회복되었다. ③ 냉동보존후 췌도의 포도당에 대한 동적 인슐린 분비능:냉동보존한 췌도를 해동후 3일째의 인슐린 동적 분비능은 냉동 전과 마찬가지로 자극 인슐린의 반응의 제1기와 2기가 잘 관찰되었다. ④ 냉동보존한 췌도세포 이식 후 혈당 변화:스트렙토조토신으로 유도된 당뇨병 쥐에 췌도이식 후 혈당은 냉동보존한 췌도이식군에서 대조군에 비해 혈당의 조절효과가 더 오래 지속되었다. 결론:소동물에서 단계적 온도 하강법을 이용한 췌도세포 냉동보존법을 확립하였으며 이는 기능, 구조 및 생존율에 큰 이상을 보이지 않았으므로 장차 사람의 췌도세포 동종이식시 부족한 췌도세포수를 극복하고 면역반응을 줄일 수 있는 매우 유용한 방법이 될 것으로 판단된다. Background : Although islet transplantation has been attempted to reverse the state of diabetes, achieving a critical number of islets and modulating the immune response limit the success of islet transplantation. Cryo-preservation of islets offers many important benefits for islet transplantation by collecting islets with a wide variety of HLA phenotypes and islet MHC expression. The aims of this study was to determine the optimal conditions for cryo-preservation by using a controlled cooling method and to evaluate in vitro and in vivo functional properties of the cryo-preserved islets. Methods : Collagenase-isolated, Ficoll-purified islets were cultured for 48 hours. They were aliquoted into freezing tubes (1000 islets per tube), equilibrated with 2M dimethyl sulfoxide (DMSO) in three steps, supercooled, nucleated, and controll-cooled at rate of 0.25℃/min to - 40℃ prior to storage at - 196℃. Rapid thawing and removal of DMSO with 0.75 M sucrose preceded 48 hour of culture and the morphology, viability, glucose-induced insulin secretion, and in vivo function of rats transplanted with cryopreserved islets was reexamined. Results : ① Recovery was 90.2±0.2%, 85.7±0.1% and 81.7±0.1% immendiately after, 24 hours and 72 hours after thawing respectively. The viability was 60±5%, 80±5%, 90±5% immediately after, 24 hours and 72 hours after thawing respectively. ② The glucose-stimulated-insulin secretion (GSIS) tended to decrease immediately after thawing, but GSIS increase to the level of pre-cryopreservation 72 hours after thawing. ③ The in dynamic GSIS, the first and the second phase of insulin secretion were well preserved in islets cultured for 72 hours after thawing. ④ The cryopreserved islets were cultured for 3 days and transplanted into renal sub-capsular space of streptozotocin (STZ) induced diabetic rats. The duration of normoglycemia in the STZ-induced diabetic rats transplanted with cryopreserved islets was significantly longer than of the fresh islets. Conclusion : The optimal condition of cryopreservation using the controlled cooling method was established in rat pancreatic islets. This cryopreservation method can be a feasible approach for human islet transplantation (J Kor Diabetes Asso 26:64~74, 2002).