Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation

Kim, S.-H.,Lee, S.-O.,Park, I.-A.,Park, S.J.,Choi, S.-H.,Kim, Y.S.,Woo, J.H.,Park, S.-K.,Park, J.S.,Kim, S.C.,Han, D.J. Blackwell Publishing Inc 2010 Transplant infectious disease Vol.12 No.2

<P>S.-H. Kim, S.-O. Lee, I.-A. Park, S.J. Park, S.-H. Choi, Y.S. Kim, J.H. Woo, S.-K. Park, J.S. Park, S.C. Kim, D.J. Han. Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation.Transpl Infect Dis 2010: <B>12:</B> 113–119. All rights reserved</P><P>Background</P><P>The presence of latent tuberculosis (TB) infection (LTBI) should be evaluated before kidney transplantation. Although a new T cell-based assay for diagnosing LTBI gave promising results, this assay has not yet been compared with the tuberculin skin test (TST) for diagnosing LTBI in renal transplant candidates before transplantation.</P><P>Patients and methods</P><P>All adult patients admitted to a single institute for renal transplantation over a 1-year period were prospectively enrolled. A clinically predictive risk of LTBI was defined as: (i) recent close contact with a person with pulmonary TB; (ii) abnormal chest radiography; (iii) a history of untreated or inadequately treated TB; or (iv) a new infection (i.e., a recent conversion of TST).</P><P>Results</P><P>Of 209 renal recipients, 47 (22%) had a positive TST≥5 mm, 21 (10%) had a positive TST≥10 mm, 65 (30%) had a positive T-SPOT.<I>TB</I> test, and 25 (12%) had an indeterminate T-SPOT.<I>TB</I> test. The induration size of TST was significantly associated with a high positivity rate on T-SPOT.<I>TB</I> (<I>P</I><0.001). Agreement between T-SPOT.<I>TB</I> test and TST≥10 mm was fair (<I>k</I>=0.24, 95% confidence interval 0.11–0.36). However, neither univariate nor multivariate analysis showed any association between the clinical risk for LTBI and positivity on T-SPOT.<I>TB</I> or TST.</P><P>Conclusion</P><P>T-SPOT.<I>TB</I> test was more frequently positive than TST in renal transplant candidates. However, further longitudinal studies are awaited to determine whether the ability of T-SPOT.<I>TB</I> assay to detect LTBI in renal transplant recipients can better predict the development of TB than can TST after transplantation.</P>

1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.

Construction and Commissioning of the KSTAR Helium Distribution System

Lee, Y.J.,Park, Y.M.,Kim, Y.S.,Jang, H.S.,Kwag, S.W.,Chang, Y.B.,Park, H.T.,Woo, I.S.,Song, N.H.,Bang, E.N.,Ha, T.H.,Baek, S.,Kim, M.K.,Kim, W.C.,Oh, Y.K.,Bak, J.S. IEEE 2009 IEEE transactions on applied superconductivity Vol.19 No.3

<P>The KSTAR components requiring cryogenic helium coolant for superconducting magnet operations are connected to the helium refrigeration system (HRS) through the helium distribution system (HDS), the final helium distribution station. Twenty eight cryogenic valves including 4 quench protecting valves, many sensors such as temperature sensors, pressure transmitters, and flowmeters are mounted on the system. The HDS has to control the 4.5 K supercritical helium (600 g/s) for 30 superconducting coils and their superconducting bus-lines, 55 K helium (270 g/s) pressurized to 18 bars for the thermal shields and the gravity supports, and a maximum 17.5 g/s of liquid helium for 18 current leads, provided from the HRS which has a 4.5 K equivalent cooling power of 9 kW. The helium distribution system consists of a helium distribution box (DB), 5 helium transfer lines, a PLC based helium control system (HCS), etc. The helium control system is connected to the KSTAR supervisory control & interlock system. The detailed status regarding the construction, commissioning during first cool-down, and the instrumentation & control (I&C) system are included in this paper.</P>

대학생 자녀를 둔 중년여성의 자아존중감, 자녀와의 관계, SNS 이용강도가 우울에 미치는 영향

우지원,김가은,김경미,남주희,박지인,범준희,이나경,조소연,조은경,최영윤,김석선,길민지 이화여자대학교 간호과학대학 2017 이화간호학회지 Vol.- No.51

Purpose: This study aims to identify the effects of self-esteem, relationship with children, Social Networking Services(SNS) use intensity on depression in middle-aged women with college-age children, and to provide basic data for the health promotion of middle-aged women. Method: A sample of 133 middle-aged people was purposely selected from parents of university students. Questionnaires used for this study were CES-D(The Center for Epidemiological Studies-Depression Scale), Self-esteem Scale, Relationship with Children Scale, SNS Intensity Scale. The collected data was analyzed by SPSS 21 with descriptive statistics, independent t-test, ANOVAs, Pearson correlation, multiple regression. Result: Self-esteem was correlated with relationship with children (r=0.385, p<0.01) and depression (r=-0.440, p<0.01). Relationship with children was correlated with depression(r=-0.402, p<0.01). As a result, a factor having the greatest influence on the subject's depression degree was self-esteem(β=-0.335), followed by relationship with children(β=-0.274). Conclusion: The result of this study suggested that depression in middle-aged women is closely related to self-esteem and relationship with children. This result can be used in Nursing by developing the theoretical basis for education program related to enhancing self-esteem and relationship with children.

Retention behavior of microparticles in gravitational field-flow fractionation (GrFFF): Effect of ionic strength

Woo, I.S.,Jung, E.C.,Lee, S. Pergamon Press ; Elsevier Science Ltd 2015 Talanta Vol.132 No.-

Retention behavior of micron-sized particles in gravitational field-flow fractionation (GrFFF) was studied in this study. Effects of ionic strength and flow rate as well as the viscosity of the GrFFF carrier liquid was investigated on the size-based selectivity (S<SUB>d</SUB>), retention ratio ®, and plate height (H) of micron-sized particles using polystyrene latex beads as model particles. It was found that the retention ratio of microparticles increases with increasing flow rate or the viscosity of the carrier liquid as the particles are forced away from the accumulation wall by increased hydrodynamic lift forces (HLF). On the other hand, the retention time increases (retention ratio decreases) with increasing ionic strength of the carrier liquid at the same flow rate, due to decreased repulsive interaction between the particles and the channel accumulation wall (glass in this study) allowing the particles approach closer to the wall. Results suggest the ionic strength of the carrier liquid plays a critical role in determining retention of microparticles in GrFFF as well as the viscosity or the flow rate of the carrier liquid. It was found that the resolution and the separation time could be improved by increasing the carrier viscosity and by carefully adjusting the ionic strength of the carrier liquid.

Bandgap-Graded Cu₂Zn(Sn_1-xGe_x)S₄ Thin-Film Solar Cells Derived from Metal Chalcogenide Complex Ligand Capped Nanocrystals

Kim, I.,Kim, K.,Oh, Y.,Woo, K.,Cao, G.,Jeong, S.,Moon, J. ACS AMERICAN CHEMICAL SOCIETY 2014 Chemistry of materials Vol.26 No.13

<P>We demonstrate organic residue free, bandgap-graded Cu2Zn(Sn1-xGex)S-4 (CZTGeS) thin-film solar cells based on metal chalcogenide complex (MCC) ligand capped nanocrystals (NCs). The bandgap of the CZTGeS films is tuned by varying the amount of Sn2S64- MCC ligand absorbed on the surface of the Cu2ZnGeS4 (CZGeS) NCs, without an undesirable postselenization process. Using CZGeS NCs inks with three different Sn/(Ge+Sn) ratios, bandgap-graded CZTGeS thin films are obtained via multicoating and annealing procedures. Compositional and spectroscopic analyses along the film thickness confirm that the bandgraded CZTGeS absorber layer, with a gradually increasing bandgap from the back contact to the p-n junction, is successfully accomplished. Compared with an ungraded band structured CZTGeS cell, this normal grading structure facilitates both higher short circuit current and open-circuit voltage, facilitating a power conversion efficiency of 6.3%.</P>

Eradication of Tropical Cymbidium Viruses by Repeated Shoot-Tip Culture and Ribavirin Treatment

Choi, S. O 한국식물조직배양학회 1996 식물생명공학회지 Vol.23 No.4

Melatonin enhances arsenic trioxide-induced cell death via sustained upregulation of Redd1 expression in breast cancer cells

Yun, S.M.,Woo, S.H.,Oh, S.T.,Hong, S.E.,Choe, T.B.,Ye, S.K.,Kim, E.K.,Seong, M.K.,Kim, H.A.,Noh, W.C.,Lee, J.K.,Jin, H.O.,Lee, Y.H.,Park, I.C. North-Holland 2016 Molecular and cellular endocrinology Vol.422 No.-

Melatonin is implicated in various physiological functions, including anticancer activity. However, the mechanism(s) of its anticancer activity is not well understood. In the present study, we investigated the combined effects of melatonin and arsenic trioxide (ATO) on cell death in human breast cancer cells. Melatonin enhanced the ATO-induced apoptotic cell death via changes in the protein levels of Survivin, Bcl-2, and Bax, thus affecting cytochrome c release from the mitochondria to the cytosol. Interestingly, we found that the cell death induced by co-treatment with melatonin and ATO was mediated by sustained upregulation of Redd1, which was associated with increased production of reactive oxygen species (ROS). Combined treatment with melatonin and ATO induced the phosphorylation of JNK and p38 MAP kinase downstream from Redd1 expression. Rapamycin and S6K1 siRNA enhanced, while activation of mTORC1 by transfection with TSC2 siRNA suppressed the cell death induced by melatonin and ATO treatment. Taken together, our findings suggest that melatonin enhances ATO-induced apoptotic cell death via sustained upregulation of Redd1 expression and inhibition of mTORC1 upstream of the activation of the p38/JNK pathways in human breast cancer cells.

불안정 비구골절의 수술적 치료 : 수술시 문제점 및 합병증을 중심으로 Clinical analysis of 28 cases with consideration of surgical problems and complications

김인,우영균,김용식,송석환,권순용,박수안 대한골절학회 1994 대한골절학회지 Vol.7 No.2

Authors reviewed total 28 cases of acetabular fracture with operative management followed up over 1 year. The clinical results were retrospectively analyzed with consideration of surgical prohlems and complications. The overall results were as follows: l. According to the classification by Judet and Letournel(l974), 20 cases were elementary fractures and 8 cases were associated fractures. The posterior wall fractures were most common in 9 cases. 2. Kocher-Langenbeck approach in 18 cases, ilioinguinal in 4 cases, iliofemoral in 4 cases and triradiate transtrochanteric approach in 2 cases were used. 3. The devices for internal fixation were as follows: screw only in 8 cases. plate and screw in 14 cases, plate and screw with circumferential wiring in 4 cases, wire and staple only in 1 each case. 4. The early and late complications occurred postoperatively as follows: incomplete sciatic nerve palsy 2 cases, wound infection 2 cases as early complications and posttraumatic arthritis 6 cases, avascular necrocis of femoral head 2 cases, heterotropic oscification 1 case as late complications. Two cases of sciatic nerve palsy were spontaneously recovered and 2 cases of wound infection were controlled by adequate drainage and antibiotic therapy. And then, the total hip arthroplasty were carried out for 2 cases of avascular necrosis of femoral head, and 6 cases of posttaumatic arthritis and 1 case of heterotopic ossification were under observation. 5. Postoperatively, the causes of inadequate reduction and insufficient fixation were radiographically analyzed with immediately and lastly checked plain films, of which causes in 9 cases were as follows: inappropriate approach for exposure in 4 cases, delayed operation due to major associated injury over 3 weeks in 3 cases and severe comminution in 2 cases. As a result, we reached to put an emphasis on an importatnce of preoperative planning, including the evaluation of individual fracture personality, the choice of surgical approach and the method of intemal fixation.

Performance of a Small-scale Molten Carbonate Fuel Cell Stack I: Performance of a 100W-Class Cross-flow Type Stack

Suk Woo Nam 한국화학공학회 1995 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.33 No.5