Isolation and Characterization of Antimicrobial Substance Macrolactin A Produced from Bacillus amyloliquefaciens CHO104 Isolated from Soil

( Seung Je Lee ),( Jeong Yong Cho ),( Jung Il Cho ),( Jae Hak Moon ),( Ki Deok Park ),( Young Ju Lee ),( Keun Hyung Park ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

유한세프라딘 캅셀(세프라딘 500 mg)에 대한 브로드세프 캅셀의 생물학적 동등성

조혜영,이석,강현아,오인준,임동구,문재동,이용복 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

Cepharadine is a first generation cephalosporin and has broad spectrum antibacterial activity against gram-positive and gram-negative microorganisms, through inhibition of bacterial cell wall synthesis. Cephradine is useful for treatment of infections of the urinary and respiratory tract, skin and soft tissues. The purpose of the present study was to evaluate the bioequinalence of two cepharadine capsules, Cefradine Yuhan (Yuhan Corporation) and Broadcef (Ilsung Pharmaceuticals Co. Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). The cephradine release from the two cephradine capsules ?? was tested using KP Ⅶ Apparatus Ⅱ method with various different kinds of dissolution media (pH 1.2, 4.0, 6.8 buffer solution and water). Twenty normal male volunteers, 23.10±2.90 years in age and 67.69±8.04 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After one capsule containing 500 ㎎ as cephradine was orally administered, blood was taken at predetermined time intervals and the concentrations of cepharadine in serum were determined using HPLC method with UV detector. The dissolution profiles of two cephradine capsules were very similar at all dissolution media. Besides, the phaemacokinetic parameters such as AUC_t, C_max and T_max were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_t and C_max and untransformed T_max. The results showed that the differences in AUC_t, C_max and T_max between two capsules based on the Cefradine Yuhan were -2.87%, -0.96% and -4.85%, respectively. There were no sequence effects between two capsules in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.93)∼log(1.02) and log(0.88)∼log(1.13) for AUC_t, and C_max, respectively). the 90% confidence interval using untransformed data was within ±20% (e.g., -17.54∼7.78 for T_max). All parameters met the criteria of KFDA guideline for bioequivalence, indicating that Broadcef capsule is bioequivalent to Cefradine Yuhan capsule.

항생제 혼합 시멘트 정을 이용한 장관골 감염성 불유합의 이단계 치료

조세현,정순택,박형빈,황선철,하용찬,황인환 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적: 장관골 감염성 불유합의 치료 시 항생제 혼합 시멘트 정을 이용한 이단계 재건술의 결과를 분석하고자 한다. 대상 및 방법: 1998년 1월부터 2002년 9월까지 장관골 감염성 불유합으로 이단계 재건 수술을 받은 41예 (대퇴골 26예, 경골 15예)를 대상으로 하였다. 일단계 수술에서는 광범위한 변연 절제술, 부골 제거 및 세척술과 기존 고정 장치의 제거 또는 외고정 장치로의 교환을 시행한 후 시공 (dead space)과 불유합 주변 연부조직 내 항생제 혼합 시멘트 정을 삽입하였다. 임상적 감염 증상이 호전된 평균 8.7주 (3주~32주) 후 이단계 재건술로서 Ⅰ군은 내고정 장치 (금속판 5예, 골수강내 금속정 8예), Ⅱ군은 외고정 장치 (Ilizarov 25예, Monofixator 3예)로 각각 고정하고 17예에서는 골내 이동술이나 골 연장술을 시행하였다. 양군 모두 필요한 경우 골 이식과 시멘트 정의 교환을 추가하였다. 추시 기간은 16개월에서 최장 71개월로 평균 45개월 이었다. 치료 결과는 골유합 기간 및 Paley 방법에 의한 방사선상 골유합 상태와 하지 기능으로 평가하였다. 결과: Ⅰ군 13예 전부와 Ⅱ군 28예 중 26예에서 감염의 치료와 골유합을 얻었다. 이단계 재건술 후 골 이식술, 골 소파술, 외고정 핀 교환과 각변형 교정술, 피부 이식 또는 근육 피판 회전술 등의 보조적 시술이 Ⅰ군은 평균 2회, Ⅱ군은 평균 6.2회 추가되었다. 평균 골유합 기간은 Ⅰ군이 19.3주이고 Ⅱ군이 23.1주로 내고정군이 더 빨랐다. 방사선상 Paley 골유합 상태는 Ⅰ군이 우수 8예, 양호 5예이었고, Ⅱ군이 우수 18예, 양호 7예, 보통 1예, 불량 2예 이었다 (p=0.492). 기능적 결과는 Ⅰ군에서 우수 6예, 양호 6예, 보통 1예 이었고, Ⅱ군에서는 우수 10예, 양호 13예, 보통 3예, 불량 2예 이었다 (p=0.267). 결론: 항생제 혼합 시멘트 정의 골수염 치료 효과가 관찰되었으며, 감염이 호전된 후 내고정 장치로 교환해 준 군이 외고정을 유지한 군에 비하여 추가 수술 횟수가 적고, 골유합 기간도 빨랐다. Purpose: To evaluate treatment results between internal na external fixation groups in two-stage reconstruction of infected nonunion of long bones using antibiotics-impregnated cement beads. Materials and Methods: In the first stage, preexisting hardwares were removed and radical debridement was done. The dead space was filled with antibiotics -impregnated cement beads and the nonunion sits was immobilized by external fixation, cast or skeletal traction. In the second stage, all cases were divided into two groups; the nonunion was fixed by internal fixation in group Ⅰ versus external fixation in group Ⅱ. The intervening period between the first and second stage was average 8.7 weeks (range, 3~23 weeks). Results: The follow-up period was average 45 months (range, 16~71 months). Infection control and bone union were achieved in all 13 cases of group Ⅰ. Infection recurred in two of 28 cases in group Ⅱ, one underwent above-knee amputation and the other case was lost in follow-up. The mean number of supportive operations including repeated curettage, augmentation and change of infected pins, angular correction, and soft tissue flap was average 2 and 6.2 times respectively in group Ⅰ and group Ⅱ. Bony union period was average 19.3 and 23.1 weeks in each group. According to Paley's classification, group Ⅰ was similar to group Ⅱ in bony and functional result (p>0.05). Conclusion: Antibiotics-impregnated cement beads provided positive effect on infection control. Internal fixation group showed less number of additional operations and earlier bony union than external fixation group.

용융염산화 반응기에서 기체체류량 및 기-액 흐름특성

조용준,양희철,은희철,유재형,김준형 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.5

용융염산화는 혼성폐기물과 유해폐기물을 효과적으로 산화 및 분해할 수 있는 소각대체기술중 하나이다. 본 연구에서는 직경이 0.079 m이고 높이가 0.653 m인 용융염산화 반응기에서 기체체류량 및 기-액 흐름특성에 대한 연구를 수행하였다. 액상으로 용융탄산나트륨을 기상으로 건조된 공기를 사용하였으며 기체유속(0.05-0.22 m/s)과 용융염온도(870-970℃) 변화가 기체체류량 및 기-액 흐름특성에 미치는 영향을 규명하였다. 기체체류량은 용융염 온도가 상승함에 따라 증가하였는데, 이는 용융염 온도의 증가로 인해 용융된 탄산나트륨의 점도와 표면장력이 감소하였기 때문이다. 실험에서 얻어진 기체체류량 데이터를 drift-flux 모델에 적용하여 용융염반응기 내의 흐름특성을 규명할 수 있었으며, 이를 통해 흐름영역에 따른 기체체류량을 정확하게 예측할 수 있었다. Molten salt oxidation is one of the most promising alternatives to incineration that can be used to efficiently destroy the organic components of mixed wastes and hazardous wastes. In this study, the gas holdup and gas-liquid flow characteristics are investigated in the molten salt oxidation reactor (0.076 m D×0.653 m H.). Effects of input air velocity (0.05-0.22m/s ) and molten salt temperature (870-970℃) on the gas holdup and flow characteristics have been studied. Molten carbonate as the liquid phase and air as the gas phase have been used in this study. The gas holdup increases with increasing molten salt temperature due to the decrease of viscosity and surface tension of molten carbonate. The experimentally obtained gas holdups in the molten salt reactor have been well described and characterized by means of drift-flux model. The gas holdups with variation of the flow regime have been well predicted.

웹데이터베이스를 이용한 기업정보화의 전략적 추진에 관한 연구

조세형,김용원 한국정보전략학회 1999 한국정보전략학회지 Vol.2 No.1

The Internet and World Wide Wed(WWW) hold many possibilities for the strategic use of global information technology in the business dimension including business informatization. The proliferation of the Internet and on-line information services has resulted in the integration environment of distribuied computing resources, especially databases. WWW, a hypermedia system for browsing and retrieving Internet resources, use gateway technology to integrate distrbuted databases on platform-independent architecture. In this paper, we present a conceptual model of business information system based on Web DB. The model suggests WWW-based business computing that is already seen as a new competitive business weapon. Finally, prerequisite conditions are consedered for the successful use of this model.

배양 표피세포 이식의 생착에 관한 연구

조용근,홍성표,장충현,이두형 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.1

We cultured the keratinocytes from different donors for 3 weeks and the cultured keratinocyte sheets were applied to the 15 cases of patients who had hypertrophic scars on their extremities. (auto-??allografting) And the applied keratinocyte sheets were studied for the adaptation, growth ?? differentiation with light and electron-microscopy. The results were as follows; 1. Grafted epidermal cells started differentiation from second week post-grafting and showed a similar appearance to the normal epidermis at fourth week post-grafing. 2. Keratohyalind granules were seen from fourth week post-grafting and showed a normal appearance at fifth month post-grafting. 3. Immature desmosome-tonofilament complexes were seen at cultured epidermal sheet and showed a normal appearance at fifth month post-grafting. 4. Rete ridges showed its appearance at fourth week post-grafting and showed a normal appearance at fifth month post-grafting. 5. Fine structures of epidermo-graft bed junction showed a discontinuous appearance at second week post-grafting and showed a normal continuous appearance at fifth month post-grafting. In summary, the 'take' of grafted epidermal sheets begins at fourth week post-grafting and completed at fifth month post-grafting.

중소기업 전자상거래의 활성화전략에 관한 연구

조세형,김승철,정용균 한국중소기업학회 2004 中小企業硏究 Vol.26 No.2

전자상거래에 관한 대부분의 선행연구들은 기업의 전자상거래 채택 및 확산 요인에 초점을 두었으며, 대기업 중심의 자료를 바탕으로 연구가 수행되었다. 그러나, 기업에서의 전자 상거래 활용은 선택이 아닌 기정 사실화되었고 이제는 어떻게 활용하는가에 초점을 두어야할 때이다. 한편, 대기업을 대상으로 한 연구결과가 중소기업에 지침으로 제공되고 있으나, 중소기업이 단순히 대기업의 축소판이 아니며 많은 점에서 고유의 특성을 지니고 있기 때문에 중소기업 자체의 연구가 필요하다. 본 연구는 세 가지 측면에서 전자상거래에 환한 선행연구를 확장한다. 첫째는 많은 선행연구들이 주로 정보기술의 채택 및 확산이란 차원에서 전자상거래를 다루어 온 것에 비하여 본 연구에서는 전자상거래의 실행을 성과와 연관시켜 다룬다. 둘째는 국가경제에 커다란 비중을 차지하고 영향을 미치는 중소기업에 초점을 둔다. 끝으로, 중소기업에 적합한 전자상거래 활성화 전략을 제안하고자 한다. 연구결과 중소기업에 적합한 특유의 전자상거래 활용형태는 없으며, 기업상황에 적합한 활용형태를 찾는 것이 필요할 것으로 보인다. 한편, 정보시스템 성숙도 수준에 따라 IC실행형태가 성과에 미치는 영향에 차이가 있음을 발견하였다. 예를 들어, 비용우위 전략은 성숙도가 낮을수록 가치시스템형 IC는 성숙도가 높을수록 높은 성과를 나타냈다. Business firms are increasingly taking part in online business activities through internet as the size of the e-commerce market is rapidly growing in the recent years. Electronic commerce (EC) activities now become an essential part of the business strategy for survival as well as growth for both large enterprises and small- to medium-sized enterprises (SMEs). Previous studies on e-commerce mostly focused on the factors leading to adoption and diffusion of EC by business firms, and also have been carried out with the data from big enterprises. More often than not, the results obtained from the large business firms are used to provide the guidelines for SMEs. SMEs are, however, different from large business firms in many aspects, and need to be studied on their own. This paper extends the previous researches on EC in three aspects. Firstly, we study the e-commerce issues in the area of implementation in relation to EC performance, beyond the adoption and diffusion of IT technology which has been the research issues in many previous researches. Secondly, we focus on small and medium enterprises which comprise a large portion of national economy with significant influence. Finally, we propose desirable EC strategies for SMEs upon consideration of SMEs' characteristics.

레보프라이드 정(레보설피리드 25 mg)에 대한 레보피드 정의 생물학적 동등성

조혜영,강현아,문재동,이용복 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

Levosulpiride is the levo-enantiomer form of racemic sulpiride, a benzamide derivative selectively inhibiting dopaminergic D_2 receptors at the trigger zone both in the central nervous system and in the gastrointestinal tract. The purpose of the present study was to evaluate the bioequivalence of two levousulpiride tablets. Levopride (SK Pharmaceutical Co. Ltd.) and Levopid (Dae Won Pharmaceutical Co. Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). The levosulpiride release from the two levosulpiride tablets in vitro was tested using KP Ⅶ Apparatus Ⅱ method with various different kinds of dissolution media (pH 1.2, 4.0, 6.8 buffer solution and water). Twenty eight normal male volunteers, 23.82 ±3.26 years in age and 69.13 ±8.58 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After one tablet containing 2.5 ㎎ of levousulpiride was orally administered, blood was taken at predetermined time intervals and the concentrations of lovosulpiride in serum were determined using HPLC method with fluorescence detector. The dissolution profiles of two levosulpiride tablets were very similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_t, C_max and T_max were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_t, and C_max and untransformed T_max. The results showed that the differences in AUC_t, C_max and T_max between two tablets based in the Levopride were -1.17%, 1.20% and -1.09%, respectively. There were no sequence effects between two tablets in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.93)∼log(1.07) and log(0.90)∼log(1.14) for AUC_t and C_max, respectively). The 90% confidence interval using untransformed data was within ±20% (e.g., -19.47 ∼16.20 for T_max). All parameters met the criteria of KFDA guideline for bioequivalence, indicating that Levopid tablet is bioequivalent to Levopride tablet.

국제유도의 스포츠화 과정에 관한 고찰

조용철,이운형 龍仁大學校 武道硏究所 2001 武道硏究所誌 Vol.12 No.1

Under the realization that Judo gets more recognized as an international sport, this study, this study is aimed to take a close look at the Judo's development process to an international sport and changes resulting from the development. The study has come to the following four conclusions. First, along with Judo's transition into an international sports, the International Judo Federation (IJF) is also changing from a Japan-centered organization to a more international body. Judo has positioned itself as an international sport with the help of the IJF establishment, the regular holding of international Judo contests and the Judo's selection as an official Olympic sport event. Secondly, Judo's free practice style indicates that it still preserves martial art aspects, but the possibility of getting injured becomes minimized. In other words, it took one step further toward an international sport. Since the foundation of the IJF, Judo's techniques have been globally standardized. Thirdly, Judo games are shifting from defense-oriented to offense-oriented as game rules are in favor of offensive players over defensive ones. Fourthly, mindful of the general perception that it would be better to expand score zones, enable clear and far judgment, and turn a Judo match to a more offense-oriented one, the IJF has exerted its best efforts go make the Judo game rules more rational, realistic and simpler. It has yielded fruitful results. As mentioned above, Judo is a sport, which developed from a martial art. In the process of transition, however, it might lose its unique martial art features, which are commonly shared by oriental sports, Without martial art aspects, Judo would bo no different from other western sports. Therefore, in future studies, I would like to focus on problems looming with respect to the Judo's transformation into an international sport, and present solutions.

비접촉 온도센서를 이용한 2차원 열화상 측정 시스템 개발

조용진, 임형민 忠北大學校 農業科學硏究所 2017 農業科學硏究 Vol.33 No.1

A thermal imaging system has been developed to measure the temperature on surface, using non-contact temperature sensor. Also, the developed system can monitor the temperature based on Zigbee communication for ubiquitous sensor network. The measurement of temperature was performed on the pot plant depending on the moving speed of frame. The optimal speed for frame movement was 1.4 m/ s. The developed system might provide as a basic study which predict the location of crops according to the difference of temperature between soil and crop.