苛性소다 處理에 依한 밀짚의 飼料價値 增進 硏究

辛炯泰,金贊昊,金鍾奭,宋永祿,林泰鎭 成均館大學校 1982 論文集 Vol.32 No.-

Nylon bag dry matter digestibility (NBDMD, %), nylon bag organic matter digestibility (NBOMD, %) and chemical compositions were analysed to evaluate the nutritive values of 5 varieties of NaOH-treated wheat straws (Jangkwang, Wonkwang, Suwon 210, Suwon 211, Naemil) by using Korean native male goats. Three grams of dried samples by grinding with 2.45㎜ Wiley mill screen were put into a bag 9×14㎠ made of 100 mesh nylon. These bags were put into the ventral sac of the rumen and taken out at the end of 72 hours. They were washed, dried in an oven at 105℃ for 24 hours and weighed “NBDMD” (%) and ashed in an muffle furnace at 600℃ or 24 hours and weighed “NBOMD”(%). The results which were obtained with this experiment were as follows: 1. Nylon bag dry matter digestibility (NBDMD, %) and nylon bag organic matter digestibility (NBOMD, %) of NaOH-treated wheat straws were highly significant different (p<0.01) in different levels of NaOH treatment and of different varieties of wheat straws. 2. The relationship between total mean NBDMD (%, Y_1) of 5 varieties of wheat straws and NaOH concentration (%, X) at 72 hours rumen incubation was: Y_1=34.57+3.56X(r=0.92^**) 3. The relationship between total mean MBOMD (%, Y_2) of 5 varieties of wheat straws and NaOH concentration (%, X) at 72 hours rumen incubation was: Y_2=32.02+2.46X(r=0.92^**) 4. The orders of NBDMD(%) and NBOMD(%) for 5 varieties of wheat straws were Suwon 210, Suwon 211, Naemil, Jangkwang, Wonkwang and Suwon 211, Suwon 210, Naemil, Jangkwang, Wonkwang, respectively. 5. The optimum level of NaOH for improving NBDMD(%) from 5 varieties of wheat straws, on the basis of Blaxter et al. (1961), was 6% NaOH.

간질환에서의 Cellulose acetate 전기 영동 분획변화에 대한 연구

신태호,이형동,김창세 朝鮮大學校 醫學硏究所 1979 The Medical Journal of Chosun University Vol.- No.-

Cellulose acetate electrophoretic fractions of serum proteins were performed and the following results were obtained: 1) The mean values for the electrophoretic fraction of serum proteins in control groups (normal) were 3.29±0.6gm/100㎖ (48.36±6.65%), 0.22±0.09gm/100㎖ (3.49±1.32%), 0.76±0.23gm/100㎖(10.58±2.87%), 0.99±0.20gm/100㎖ (14.38±3.27%), and 1.50= 0.34gm/100㎖ (21.28±4.64%), respectively, for the albumin, α_1-, α_2-,β-, and γ-glo-bulin fractions. 2) The mean values for the electrophoretic fractions of serum proteins in liver cirrhosis group were 2.41±0.46gm/100㎖ (34.11±8.27%) 0.28±0.15gm/100㎖ (4.06±2.19%), 0.64±0.24gm/100㎖ (9.08± 3.67%), 1.00±0.34gm/100㎖ (14.08±4. 23%), and 2.74±0.99gm/100㎖(38.78±9.91%), respectively, for the albumin, α_1-, α_2-,β-, and γ-globulin fractions. It was also found that the changes observed in liver cirrhosis were more marked than in control group particularly other forms of liver disease-the most charac-teristic abnormalities being decreased concentrations of albumin and increased concentra- tions of γ-aglobulin. 3) The mean values for the electrophoretic fractions of serum proteins in heptitis group were 2.67±0.76gm/100㎖ (41.95±10.43%), 0.30±0.15.gm/100㎖ (4.60±2.62%), 0.73±0.28gm/100㎖ (11.19±4.35%), 0.88±0.36gm/100㎖(14.03±4.33%), and 1.74±0.55gm/100㎖ (27.51±6.11%), respectively, for the albumin, α_1-, α_2-,β-, and γ-globulin fractions. It was found that the decreased concentration of albumin observed in hepatitis was more marked than in control group. 4) The mean values for the electrophoretic fractions of serum proteins in hepatoma group were 2.66±0.58gm/100㎖(38.04±8.92%), 0.31±0.13gm/100㎖(4.53±2.39%), 0.75±0.31gm/100㎖(10.79±3.68%), 1.11±0.46gm/100㎖(16.00±5.43%), and 1.96±0.63gm/100㎖ (28.73±8.13%), respectively, for the albumin α_1-, α_2-,β-, and γ-globulin fractions. It was found that decreased concention of albumin and increased concentration of γ-globulin fractions in hepatoma were more marked than in control group. 5) The mean values for the electrophoretic fractions of serum proteins in cholecystopa- thy group were 2.55±0.65gm/100㎖(40.63±11.56%), 0.25±0.14gm/100㎖(4.13±2.03%), 0.82±0.25gm/100㎖(12.69±3.49%), 0.97±0.31gm/100㎖(14.75±3.21%), and 1.77±0.75gm/100㎖(27.25±9.51%), respectively, for the albumin, α_1-, α_2-,β-, and γ-globulin fractions. It was found that the decreased concentration of albumin in cholecystopathy was more marked than in control group. 6) The mean values for the electrophoretic fractions of serum protins in liver abscess group were 2.36±0.43gm/100㎖(35.27±5.06%), 0.58±0.28gm/100㎖(8.38±3.43%), 0.98±0.36gm/100㎖(l4.38±4.89%), 0.95±0.29gm/100㎖(13.69±3.12%), and 1.83±0.71gm/100㎖ (27.15±8.40%), respectvely, for the albumin, α_1-, α_2-,β-, and γ-globulin fractions. It was found that decreased concentration of albumin and increased concentration of α_1-globulin fractions were marked than in control group. 7) All of the clinical chemistry data, so-called liver function tests, were shown the increased concentrations except cholesterol. Most chracteristic findings were the increased concentration of serum potassium observed in liver cirrhosis and hapatoma groups.

18S-srRNA 분석에 의한 Acantbamoeba 한국 분리주들의 분류 검토

신호준,지영진,조명수,김형일,박연희,임경일 아주대학교 의과학연구소 1998 아주의학 Vol.3 No.2

Acanthamoeba spp. spread in soil, ponds, air and swimming pool, cause a granulomatous amoebic meningitis and amoebic keratitis in human and experimental animals. Their classification had depended upon morphological characteristics and physiological conditions such as thermophilic status and generation times. However, because amoeba species showed the morphological and physiological diversity in isolates, many attempts have been made on identification of Acanthamoeba spp. Restriction fragments length polymorphism (RFLP) analysis of IBS-small subunit ribosomal RNA (srRNA) was an useful tool for classification among morphologically and genetically closely-related species. In this study, 18S-srDNAs of amoebae were amplified by PCR with primer encoding 18S-srRNA gene and digested with restriction endonudeases, and RFLP analysis was applied on classification of Acanthamoeba Korean isolates (YM-4, YM-5 and YM-7), comparing with reference amoebae, A. culbertsoni, A. polyphaga and A. royreba. Trophozoites of six Acanthamoeba spp. had typical acanthopoda, but did not show any morphological differences. Cyst of Acanthamoeba sp. YM-7 was similar to that of A. polyphaga which was designated as group Ⅱ Acanthamoeba. Morphologically other amoebae belonged to group Ⅲ Acanthamoeba. Digestion of 18S-srDNA with six enzymes, Dde Ⅰ, Hae Ⅲ, Hind Ⅲ, EcoR Ⅰ, Rsa Ⅰ and Sph Ⅰ, resulted in various DNA fragments. Using the method of Nei and U (1979) for RFLP analysis, genetic divergence was not observed between A culbertsoni and Acanthamoeba sp. YM-4. Between A. culbertsoni and Acanthamoeba sp. YM-5, genetic distance was 0.070, 0.364 between Acanthamoeba sp. YM-4 and A. polyphaga, and 0.277 between Acanthamoeba sp. YM-7 and A. polyphaga. In comparison with A. culbertsoni and A. polyphaga, Acanthamoeba sp. YM-7 showed genetic distance of 0.330 and 0.154, respectively. Thus, Acanthamoeba sp. YM-7 is a similar species to A. polyphaga. Acanthamoeba sp. YM-5 is a different species from A. polyphaga, but closely-related to A. culbertsoni. Acanthamoeba sp. YM-4 can be classified as a subspecies or a strain of A. culbertsoni.

간질환에서의 Cellulose acetate 전기 영동 분획변화에 대한 연구

신태호,이형동,김창세 順天鄕大學校 1979 의대논문집 Vol.4 No.-

Cellulose acetate electrophoretic fractions of serum proteins were performed and the following results were obtained: 1) The mean values for the electrophoretic fractions of serum proteins in control groups (normal) were 3.29±0.6gm/100㎖ 48.36±6.65%), 0.22±0.09gm/100㎖ (3.49±1.32%), 0.76±0.23gm/100㎖(10.58±2.87%), 0.99±0.20gm/100㎖ (14.38±3.27%), and 1.50±0.34gm/100㎖(21.28±4.64%), respectively, for the albumin, a₁-, a₂-, β-, and r-glo-bulin fractions. 2) The mean values for the electrophoretic fractions of serum proteins in liver cirrhosis group were 2.41±0.46gm/100㎖(34.11±8.27%), 0.28±0.15gm/100㎖ (4.06±2.19%), 0.64±0.24gm/100㎖(9.08±3 67%), 1.00±0.34gm/100㎖(14.08±4.23%), and 2.74±0.99gm/100㎖(38.78±9.91%), respectively, for the albumin, a-, a₂-, β-, and r-globulin fractions. It was also found that the changes observed in liver cirrhosis were more marked than in control group particularly other forms of liver disease-the most charac-teristic abnormalities being decreased concentrations of albumin and increased concentrations of r-aglobulin. 3) The mean values for the electrophoretic fractions of serum proteins in hepatitis group were 2.67±0.76gm/100㎖(41.95±10.43%), 0.30±0.15gm/100㎖ (4.60±2.62%), 0.73±0.28gm/100㎖(11.19±4.35%), 0.88±0.36gm/100㎖ (14.03±4.33%), and 1.74±0.55gm/100㎖(27.51±6.11%), respectively, for the albumin, a₁-, a₂-, β-, and r-globulin fractions. It was found that the decreased concentration of albumin observed in hepatitis was more marked than in control group. 4) The mean values for the electrophoretic fractions of serum proteins in hepatoma group were 2.66±0.58gm/100㎖(38.04±8.92%), 0.31±0.13gm/100㎖ (4.53±2.39%), 0.75±0.31gm/100㎖(10.79±3.68%), 1.11±0.46gm/100㎖(16.00±5.43%), and 1.96±0.63gm/100㎖(28.73±8.13%), respectively, for the albumin, a₁-, a₂-, β-, and r-globulin fractions. It was found that decreasad concention of albumin and increased concentration of r-globulin fractions in hepatoma were more marked than in control group. 5) The mean values for the electrophoretic fractions of serum proteins in cholecystopathy group were 2.55±0.65gm/100㎖(40.63±11.56%), 0.25±0.14gm/100㎖(4.l3±2.03%), 0.82±0.25gm/100㎖(12.69±3.49%), 0.97±0.31gm/100㎖(l4.75±3.21%), and 1.77±0.75gm /100㎖(27.25±9.51%), respectively, for the albumin, a₁-, a₂-, β-, and r-globulin fractions. It was found that the decreased concentration of albumin in cholecystopathy was more marked than in control group. 6) The mean values for the electrophoretic fractions of serum protins in liver abscess group were 2.36±0.43gm/100㎖(35.27±5.06%), 0.58±0.28gm/100㎖(8.38±3.43%), 0.98±0.36gm/100㎖(14.38±4.89%), 0.95±0.29gm/100㎖(l3.69±3.12%), and 1.83±0.71gm/100㎖(27.15±8.4096㎖), respectvely, for the albumin, a₁-, a₂-, β-, and r-globulin fractions. It was found that decreased concentration of albumin and increased concentration of a₁-globulin fractions were marked than in control group. 7) All of the clinical chemistry data, so-called liver function tests, were shown the increased concentrations except cholesterol. Most chracteristic findings were the increased concentration of serum potassium observed in liver cirrhosis and hapatorna groups.

요양소에 수용중인 정신장애자의 입소전 치료방법에 관한 연구

신병호,윤진상,이충경,이형영 大韓神經精神醫學會 1988 신경정신의학 Vol.27 No.5

In Korea, a large number of the mentally ill are still institutionalized in asylums where they can not receive modern psychiatric treatments. This survey was designed to investigate previous treatment methods of these psychiatric patients and to offer basic data in order to develop modern psychiatric delivery system for patients institutionalized in asylums. The 694 subjects who could give cooperative informations wee selrected form 6 asylums which are located in the Kwang-ju and Chonnam area. The data on this survey was collected by investigator's interview from Mar. 30, 1987 to Apr. 10, 1987. The previous treatment methods were divided into 8 groups(modern psychiatric treatment, asylym, Korean shamanic rite, religious healing, herb medicine, drug store, others and no treatment) and multiple responses were allowed. The methods were studied relating to the following psychiatric and sociodemographic factors : psychiatric diagnosis, age at the time of institutionalization, morbid duration before institutionalization, sex, occupation, residence, religion, economic status, marital status, educational level, and guardian. 49% of the patients had no experienced any previous treatment for their mental illness. 35% of the patients had been given modern psychiatric treatment and 23% had been previously institutionalized in asylums. The other treatment methods were reported in less than 10% of the patients. There were significant relationships between the choice of treatment methods and the psychiatric and sociodemographic factors.

苛性소다 處理에 依한 볏짚 Silage 의 飼料價値 增進硏究

辛炯泰,朴昌學,金章鎬,金贊昊 成均館大學校 1981 論文集 Vol.30 No.-

Nylon bag dry matter digestibility (NBDMD, %) and nylon bag organic matter digestibility(NBOMD, %) were measured to evaluate the nutritive value of Tongil and Akibare rice straws ensiled after NaOH treatment by using Korean native male goats. Experimental Korean native male goat, weighing approximately 30㎏ each, was equipped with rumen cannula. Three grams of dried samples by grinding with 2.45㎜ Wiley mill screen were put into a bag 9×14 ㎠ made of 100 mesh nylon. Nylon bags were put into the ventral sac of the rumen and taken out at the end of 72 hours. They were washed, dried in an oven at 90-100℃ for 24 hours and weighed “NBDMD (%)” and ashed in an muffle furnace at 550-600℃ for 2 hours and weighed “NBOMD”(%)”. The results which were obtained with this experiment were as follows: 1. The NBDMD (%) and NBOMD (%) of NaOH-treated Tongil and Akibare silage were highly significant different (p〈0.01) in different levels of NaOH and variety of rice straw, but NBDMD(%) and NBOMD(%) were not affected by moisture content of rice straw silage (p〉0.05). 2. The relationship between NBDMD (Y_1, %) of Tongil and Akibare rice straw silage and NaOH concentration (X, %) at 72 hours rumen incubation was: Tongil rice straw silage (50%) : Y_1=4.70X+43.67 (r=0.96^*) Tongil rice straw silage (70%) : Y_1=4.61X+46.23 (r=0.99^**) Akibare rice straw silage (50%) : Y_1=4.61X+32.93 (r=0.95^*) Akibare rice straw silage (70%) : Y_1=4.97X+35.68 (r=0.97^*) 3. The relationship between NBOMD (Y_2, %) of Tongil and Akibare rice straw silage and NaOH concentration (X, %) at 72 hours rumen incubation was: Tongil rice straw silage (50%) : Y_2=4.74X+40.21 (r=0.97^*) Tongil rice straw silage (70%) : Y_2=4.05X+43.55 (r=0.99^**) Akibare rice straw silage (50%) : Y_2=4.24X+30.26 (r=0.95^*) Akibare rice straw silage (70%) : Y_2=4.34X+30.27 (r=0.97^*) 4. The optimum level of NaOH for improving NBDMD (%) and NBOMD (%) from Tongil and Akibare rice straw silage, on the basis of Blaster et al (1961), was 3% NaOH for Tongil and 4% NaOH for Akibare. But the optimum level of NaOH for rice straw silage, on the basis of Dr. McCullough's theory, was 6% NaOH for Tongil and Akibare. 5. The NBDMD (%) of ensiled NaOH-treated rice straw were more improved 20-30% than freshly-treated rice straw. It is concluded that the nutritive value of stored straw is higher than that of freshly-treated material.

건설장비와 농기계에서 배출되는 연도별 대기오염 배출량 변화추세

신문기,김호정,장영기,홍지형 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.6

The annual air pollution emissions from construction and agricultural equipments were estimated from 1987 through 2000. The annual numbers and operation hours of 5 major construction equipments (Excavator, Bulldozer, Loader, Fork lift, Crane) and 3 major agricultural equipments (Power Tiller, Agricultural Tractor, Combine) were investigated for emission estimation. And monthly variation factors of operation hours of construction equipment were investigated too. The NOx emission from construction equipment have been gradually increased since 1987 to 1997, but sharply decreased as -45% in 1998 due to economic crisis in Korea. The NOx emission was estimated as 64,300 ton/year from construction equipment, and as 23,300 ton/year from agricultural equipment in 2000.

심첨부 비후 심근증에서 심근 내 미세 관상동맥의 혈류 예비력 평가 : 경흉부 도플러 심초음파를 이용한 연구 Study Using Transthoracic Doppler Echocardiography

윤호중,박철수,신재원,임상현,조은주,정해억,전희경,오용석,정욱성,김재형,최규보,홍순조 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.3

비강 및 비인두에 발생한 도립유두종과 편평상피암종의 p53단백 및 세포증식능에 관한 연구

한주호,윤기중,이재규,신대균,박근호,조향정,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

The inverted papilloma and squamous cell carcinoma are common neoplasia in the sinonasal cavity and nasopharynx, but the incidence of these tumors are very low and the study on the oncogenesis or biological activity of the tumor cells are not well known. This study was designed to evaluate the oncogenic roles of the p53 gene and the proliferative activity of the tumor cells in the inflammatory polyp, inverted papilloma and squamous cell carcinoma. The experiment was carried by the immunohistochemical stains about the p53 protein, PCNA and Ki-67, histochemical stain about the AgNORs. and flow cytometric analysis about the DNA ploidy using the formalin fixed paraffin embedded tissues. The frequency of the expression of p53 protein was 0%(0/16 cases) in the inflammatory polyps, 18.8% (3/16 cases) in the inverted papillomas, and 87.5%(14/16 cases) in the squamous cell carcinomas. The labelling index(%) of the PCNA and Ki-67 was 5.3% and 3.3% in the inflammatoy polyps, 29.6% and 25.2% in the inverted papillomas, and 51.9% and 36.8% in the squamous cell carcinoma. The expression of the PCNA and Ki-67 was distributed in the periphery of the tumor islands of the inverted papilloma and was distributed in the both of center and periphery of the tumor islands of the squamous cell carcinoma. The number of nuclear AgNORs was increased in the order of inflammatory polyps (0.96), inverted papillomas(1.34) and squamous cell carcinoma(2.09). The frequency of the DNA aneuploidy was 0%(0/16 cases) in the inflammatory polyps, 18.8%(3/16 cases) in the inverted papillomas. and 12.5%(2/16 cases) in the squamous cell carcinomas. Above results indicates that the changes of the p53 gene and proliferative activity of the tumor cells are involved on the oncogenesis and the biological activity of the inverted papilloma or squamous cell carcinoma in the nasopharynx and sinonasa cavity.

스포츠 현장에서 사고 분쟁해결을 위한 법적 구조 및 그 효용성 분석

박형상,육조영,김우규,이재봉,유관호,이무연,박태민,이신언 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

1. The purpose of this study is to reflect reality of sports to legal structure. This author classified types of sports related accidents as match accidents, school accidents, leisure and living sports accidents. Out of such accidents common factors such as 'the provider of virtue' and 'user' could be deduced and this author reviewed what kinds of obligations must be shouldered by these people respectively. In particular this author stressed the fact that disproportionate emphasis is placed on malfeasance in disposition of dispute over the sports accidents. Malfeasance is a method for seeking relief through money after the accident. However default in an obligation enables supplementary completion when it is not performed or imperfectly performed. Thus if object which must be performed is providing safety then it is possible to secure safety prior to accident. Furthermore at such time mistake of the debtor can not become a factor. For this reason this line of thinking has the following merits: (1) In comparison with constitution of malfeasance default of an obligation has long prescription. It seems that such fact was a motivation for saving obligation for safety consideration by making it fall under the principle of faith. (2) Default of an obligation is not an obligation for ordinary attention which is cause of malfeasance but it takes up issue with obligation for attention required for performance. Game organization, sponsor and spectators etc. shares certain sense of identity and are demanded to have manners. As method for evaluation of such relationship of faith it may be possible to impose a special kind of obligation for attention and it will be close to constitution of default of an obligation. (3) Imposing obligation for provision clarifies that such responsibility belongs to game organizations promoting sports to which anyone can participates or to national or public organizations, sponsor, schools which induced such sports as a part of its educational programs, installer who made people use it as leisure facility or person in charge for its management. (4) It is also applicable to umpires, players or spectators who use field of sports. It is possible to reflect reality whereby these people specifically relate themselves to sports. In consequence it will be possible to make people feel the necessity of expanding the principals of sports from 'doers' to 'watchers.' (5) The obligation of provision is an obligation under the principle of faith therefore mutual negotiation may be expected to clarify such fact. It may be also evaluated by effectuation of users' self-determining right. 2. If the above mentioned structure is provided then, although it is limited to sports, students who are subjects of instruction, seminar attendants, and furthermore players who finished games may be obligated to bear responsibility for safety consideration under the principle of faith. Accordingly level of relief for considerable number of victims may be increased as cases of treating even borrowed materials as default of obligation being expanded. 3. As scope of responsibility becomes more clearer it will be possible to prevent disputes while securing more safety as rules and regulations become more clearly enacted or new contract is signed or existing contract is revised.