<i>Prunella vulgaris</i> Suppresses HG-Induced Vascular Inflammation via Nrf2/HO-1/eNOS Activation

Hwang, Sun Mi,Lee, Yun Jung,Yoon, Jung Joo,Lee, So Min,Kim, Jin Sook,Kang, Dae Gill,Lee, Ho Sub Molecular Diversity Preservation International (MD 2012 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.13 No.1

<P>Vascular inflammation is an important factor which can promote diabetic complications. In this study, the inhibitory effects of aqueous extract from <I>Prunella vulgaris</I> (APV) on high glucose (HG)-induced expression of cell adhesion molecules in human umbilical vein endothelial cells (HUVEC) are reported. APV decreased HG-induced expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. APV also dose-dependently inhibited HG-induced adhesion of HL-60 monocytic cells. APV suppressed p65 NF-κB activation in HG-treated cells. APV significantly inhibited the formation of intracellular reactive oxygen species (ROS). HG-stimulated HUVEC secreted gelatinases, however, APV inhibited it. APV induced Akt phosphorylation as well as activation of heme oxygenase-1 (HO-1), eNOS, and nuclear factor E2-related factor 2 (Nrf2), which may protect vascular inflammation caused by HG. In conclusion, APV exerts anti-inflammatory effect via inhibition of ROS/NF-κB pathway by inducing HO-1 and eNOS expression mediated by Nrf2, thereby suggesting that <I>Prunella vulgaris</I> may be a possible therapeutic approach to the inhibition of diabetic vascular diseases.</P>

Anti-Proliferative Effect of an Aqueous Extract of <i>Prunella vulgaris</i> in Vascular Smooth Muscle Cells

Hwang, Sun Mi,Lee, Yun Jung,Lee, Yong Pyo,Yoon, Jung Joo,Lee, So Min,Cha, Jeong Dan,Choi, Kyung Min,Kang, Dae Gill,Lee, Ho Sub Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>The abnormal proliferation of vascular smooth muscle cells (VSMCs) in arterial walls is an important pathogenic factor of vascular disorders such as diabetic atherosclerosis. We have reported the anti-inflammatory effect of an aqueous extract from <I>Prunella vulgaris</I> (APV) in vascular endothelial cell. In the present study, APV exhibited inhibitory effects on high glucose-stimulated VSMC proliferation, migration, and invasion activities, inducing G<SUB>1</SUB> cell cycle arrest with downregulation of cyclins and CDKs and upregulation of the CKIs, p21<SUP>waf1/cip1</SUP> and p27<SUP>kip1</SUP>. Furthermore, APV dose dependently suppressed the high glucose-induced matrix metalloproteinase activity. High glucose-induced phosphorylation of ERK, p38 MAPK, was decreased by the pretreatment of APV. NF-<I><I>κ</I></I>B activation by high glucose was attenuated by APV, as an antioxidant. APV attenuated the high glucose-induced decrease of nuclear factor E2-related factor-2 (Nrf2) translocation and heme oxygenase-1 (HO-1) expression. Intracellular cGMP level was also increased by APV treatment. These results demonstrate that APV may inhibit VSMC proliferation via downregulating ROS/NF-<I><I>κ</I></I>B /ERK/p38 MAPK pathways. In addition, APV has a beneficial effect by the interaction of Nrf2-mediated NO/cGMP with HO-1, suggesting that <I>Prunella vulgaris</I> may be useful in preventing diabetic atherosclerosis.</P>

장기투석을 받은 만성 신부전 환자에서 발생한 종양에 관한 고찰

황정화,이혜경,홍현숙,박재성,김대호,권귀향,최득린,황승덕,이희발 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

The authors tried to evaluate tumor occurrence in long-term dialysis patients with chronic renal failure. Among 359 patients, 20 patients (about 5.6%) were diagnosed with malignancy during long-term dialysis from the period of 1983 to 1995 at our nephrology department. The ultrasonographic and computed tomographic findings including the clinical features of 20 patients that were retrospectively reviewed. The mean age of the patients was 53 (37-75)years old and the ratio of male to female was 9:1. Among the 20 cases, 7 cases of hepatoma (35%) were developed. Among them, urinary tract tumors such as renal and bladder cancer were developed in 4 (20%) and 2 (10%) of the cases. Other malignant tumors were lymphoma, stomach cancer, uterine cervical cancer, cholangiocarcinoma, lung cancer, meningioma, and acoustic neuroma one case of each (each of 5.3%). The most common tumor in patients with chronic renal failure, who were receiving long-term dialysis, was hepatoma and the second most common tumor was cancer of the urinary tract such as kidney and bladder.

프리캐스트 포스트텐션 콘크리트 넓은 보-기둥 내부 접합부의 비탄성 거동

황재환,최윤철,김영찬,김대중,임재형,권기혁,문정호,이리형 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

The purpose of this paper is to evaluate the response of Precast Prestressed Concrete Interior Wide Beam-Column Joint subjected to cyclic lateral loading. To this end, the experimental investigation was performed with six half-scale specimens of interior connection. The design parameters are the beam to column depth ratio, and the number of tendons within column core. One of the specimens, IJH-6, is modeled for the connection at the top floor of a structure. The test results showed that the specimens sufficiently resist up to limiting drift ratio of 0.035 in accordance with the provisional standard by ACI of acceptance criteria for concrete special moment frames.

안드로이드 플랫폼에서 스마트폰 블랙박스 설계 및 구현

황현정;이호섭;전대현;김동열;김진일 東義大學校産業技術開發硏究所 2012 産業技術硏究誌 Vol.26 No.-

본 연구에서는 각종 센서를 통해 교통사고 현장의 상황을 자동으로 저장, 분석할 수 있는 스마트폰 블랙박스를 설계하고 구현하였다. 기존의 웹 캠 형식의 블랙박스 기능을 탈피하고, 사용자가 휴대하기 편한 스마트 폰이라는 장점을 활용해 차량뿐만 아니라 오토바이, 자전거에도 모두 사용가능할 수 있도록 안드로이드 플랫폼을 이용하여 블랙박스 기능을 개발하였다. 또한, 사고발생시 사전에 등록한 번호로 사고위치를 SMS 문자로 전송하여 사고 상황을 노출하도록 설계되었다.

a-TN-LCD의 전기광학특성에 표면액정배향이 미치는 영향

황율연,이창훈,서대식,이보호 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.2

The characteristics of the response time and the viewing angle on twisted nematic(TN)-liquid crystal display(LCD) and amorphous (a)-TN-LCD without rubbing were investigated. To measure the Tansmission-Voltage, response time, and viewing angle characteristics, we prepared three kinds of LC cells and then studied the surface liquid crystal alignment effect. It was found that the response time on a-TN-LCD was fast compared to the TN-LCD, and the weak anchoring strength was attributed to the fast response time on a-TN-LCD. Also, we obtained the wide viewing angle characteristics on-a-LCD. We suggest that the liquid crystal alignment effect on LCD device is very important for electro-optical characteristics.

한랭작업 근로자들의 건강위해에 관한 연구

박호추,정설미,문덕환,이종태,김대환,김정호,최재일,황용식,이용희,이채언 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

In order to evaluate the status of cold exposure and its health hazards of workers at cold storage workplaces and to provide basic data for effective health care, the author measured core temperature and also observed clinical symptoms and signs, past and present history, and general health examination data on 99 cold exposed workers for exposure group and 96 non-exposed workers for control group working at 2 food refrigerating companies in Pusan area from January 6, 1998 to February 24, 1998. The results were as follows: 1. There was statistically significant difference in water intake between the exposure and control group and increased urine volume, urine frequency in exposure group without statistically significant difference. 2. Past and present illness in exposure group were hypertension (18.2%), hepatopathy (8.1%), gastro-intestinal disease(7.1%), arthritis (4.0%), intervertebral disc herniation(4.0%), and so on, and hypertension, arthritis was statistically significant difference compared to control group. 3. Symptoms in exposure group were fatigue(89.9%), headache (64.6%). drowsy(63.6%), neck stiffness(59.6%), excessive food intake(59.6%). general weakness(58.6%), hunger(58.6%), numbness(54.5%), and so on, and there was statistically significant difference between the exposure and control group except fatigue, drowsy. 4. As results of clinical test abnormality rate of the systolic, diastolic blood pressure and electrocardiogram were significantly higher in exposed group than control. 5. Core temperature in exposure group was statistically significantly lower than control group and the highest statistically significant inverse correlation with the working hours and working frequency of daily mean cold storage. As above results, the author suggested that the further studies should be conducted to evaluate the health status of workers about chronic health effects in cold workplaces and to establish effective health care methods for them.

민어(Miichthys miiuy)의 卵發生過程 및 仔稚魚 발육단계에 따른 형태발달

한경호,오성현,황동식,조영현,서대철 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

본 연구는 2000년 8월 19일 전라남도 신안군 임자도 연안에서 2각망으로 어획된 민어 어미를 인위적인 방법으로 수정하여 부화 사육하면서 관찰한 卵發生 過程 및 仔稚魚 형태변화는 다음과 같다. 1. 민어의 受精卵은 구형의 분리 부성난으로 알의 크기는 0.96~1.08㎜(평균 1.01㎜, n=50)였고, 크고 작은 油球가 1~5개(0.06~0.34㎜) 존재하였다. 2. 사육수온 25.2~28.4℃에서 受精 후 17시간 10분에는 배체가 卵膜을 뚫고 머리부터 孵化하기 시작하였다. 3. 孵化 직후 仔魚의 전장은 2.36~2.63㎜(평균 2.48㎜, n=10)로 전장의 45.0~47.0%에 달하는 큰 卵黃(장경 1.11~1.20㎜, 단경 0.92~0.96㎜)을 가지고 있으며 筋節은 7~8+ 17~19=24~27개였다. 4. 孵化 후 3일째 仔魚는 전장 3.98~4.32㎜(평균 4.16㎜, n=10)로 입은 열려있었고, 이 시기에 섭이활동이 활발하게 관찰되면서 卵黃이 완전히 흡수되어 後期仔魚期로 이행되었다. 5. 孵化 후 10일째 後期仔魚는 전장이 5.88~6.74㎜(평균 6.30㎜, n=10)로 꼬리의 말단이 굽어지기 시작하는 시기로 黑色素胞가 기저후두골 뒷부분의 체측에서 점모양 및 나뭇가지모양 으로 출현하였다. 6. 孵化 후 31일째 개체의 전장은 9.62~12.76㎜(평균 11.12㎜, n=10)로 모든 지느러미가 정수에 달하였고, 체형이나 반문이 成魚와 닮아 種魚期로 이행하였다. Egg development and morphological change of larvae of the brown croaker, Miichthys miiuy, were investigated after obtaining samples by artificial insemination. The spawning season extends from the middle to the end of August in the seas off Lim-ja Island, Cheollanam-do. The fertilized eggs were transparent, spherical, separate and they floated. Egg diameter ranged 0.96~1.08㎜ with several oil globules of 0.06~0.34㎜. Hatching occurred 17 hours 10 minutes after fertilization in 25.2~28.4℃ water temperature. The newly-hatched larvae were 2.36~2.63㎜(mean:2.48㎜, n=10) in total length(TL), had a large yolk, and 7~8+17~19=24~27 myotomes. Melanophores were distributed on the eye lens. Three days after hatching the larvae had attained 3.98~4.32㎜ in TL(mean:4.16㎜, n=10), the yolk sac was completely absorbed, and they had transformed into the postlarva stage. The larva reached the juvenile stage with all fins formed and with a complete set of fin rays(D.Ⅹ-28~31;A.Ⅱ-7~8;P. 21~23;C. 19~21;V. 25~26) on the 31st day after hatching. The juveniles were 9.62~12.76㎜(mean:11.12㎜, n=10) in total length. At this point the juvenile was similar to the adult in body form and pigmentation pattern.

Studies on processing and storage of eel, Anguilla japonica 1.Changes in free amino acids during processing : 제1보. 가공중의 유리아미노산의 변화

송대진,하진환,이응호 제주대학교 해양연구소 1982 해양자원연구소연구보고 Vol.6 No.-

산업용 이차전지의 열화진단에 관한 연구

장대경,황호석,박민기 서울産業大學校 2005 논문집 Vol.54 No.3

This paper proposes a new degradation diagnosis method of secondary battery in order to improve a battery economy. In the proposed method, a bad cell is detected by the measurement of DC internal resistance and the battery capacity is estimated by the electric discharge test in a short period of time, which can be used to determine the time to replace the battery. Through the computer simulation and actual experimentation, the proposed method's validity is confirmed by comparing and analyzing the performance. As a result, we show that it is possible to estimate the accurate capacity of secondary battery in a short space of time.