Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Cholelithiasis complicated with biliary sludge and urolithiasis in a dog

Seung-gon Lee,Dong-gun Kim,Joon-seok Lee,Ho-hyun Kwak,Hyun-sook Nam,Heung-myong Woo*, In-Chul Park**, Changbaig Hyun1,In-chul Park,Chang-baig Hyun 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.3

A 10-year-old intact female Miniature Schnauzer dog was referred with the primary complaint of persistentanorexia, remittent fever, vomiting and abdominal pain. Hemogram suggested a chronic inflammatory disease. Serumbiochemistry showed moderate hepatobiliary cellular damage with severe cholestasis. Abdominal radiography andultrasonography revealed hepatomegaly, choleliths and sludges in gall bladder and small stones in urinary bladder. Basedcholecystectomy and cystectomy, choleliths and uroliths were removed from gall bladder and urinary bladder,respectively. The clinical condition was dramatically improved after surgery.

全北地方의 藥用作物 재배 및 生産現況

郭晙洙,金昌洙,宋英恩,金柱,朴珍華,韓宗鉉,宋昊俊 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

본격적인 지방화 시대를 맞이하면서 지역특화작물의 개발과 농가소득증대를 위한 새로운 기술개발이 절실한 이때 주요 소득원작물의 하나인 약용작물의 재배 및 생산현황을 파악하여 지역특성에 맞는 새로운 작목개발 및 정책 입안 자료로 활용하고자 조사분석한 결과는 다음과 같다. 1. 생산량을 기준으로한 전북지방의 주요 약용작물을 특화계수 분석결과 총 31개작목 중 독활, 백지, 양유, 오미자, 지황 등은 핵심품목으로 길경, 두층, 방풍, 작약, 천마 등은 주산품목으로 조사되었으며 강활 등 21개 작목은 비특화 작물로 분석되었다. 재배면적을 기준으로 한결과는 독활, 백지, 양유, 지황 등이 핵심품목으로 길경, 두층, 목단, 방풍, 백출, 오미자, 의이인, 작약 등은 주산품목으로 강활 등 19개 품목은 비특화 작물로 분석되었다. 2. 주요 약용작물 재배 계획면적 조사에서는 당귀, 두층, 오미자, 독활, 천궁 등이 계획 대비 실제 재배면적의 차이가 큰 작목으로서 이들은 일반적으로 상품가격의 영향을 많이 받는 것으로 분석되었다. 3. '96 재배면적 조사 결과 전북지방의 주요 약용작물 재배 계획 면적은 총 1,692.7ha였으며 이중 독활이 426ha로 가장 많았고, 길경(283ha), 양유(179ha), 작약(165ha), 두층(154ha), 당귀(113ha), 오미자 의이인, 지황, 산수유, 천궁, 방풍 등의 순이었으며 기타작목으로 백지, 황기, 백하수오 등 28작목이 조사되었다. With the beginning of the new localization era, epoch-making techniques in local agriculture are needed to improve farm income and devlop a locally specialized crops. On the basis of these trends in local agriculture, the status quo of production in medicinal plants which are one of the major income crops were surveyed and analyzed the data for developing a new crops and drafting a policy. The major results are as follows; 1. The results analyzed by Location Quotient of production rate in Chonbuk were classified three catagories. 5 items including Aralia continentalis K_ITAGAWA among 31 items were so rted out as a core crops, another 5 items including Platycodon grandiflotum(J_ACQ.) A.DC. were as a main crops and other 21 items were as a non-specialized crops. Analyzed by cultivation area, 4 items were sorted out as a core crops, another 8 items were as a main crops and the other 19 items were as a non-specialized crops. 2. In comparison with the gap between intended cultivation area and real cultivation area, 5 items including Eucommia ulmoides OLIVER were showed more larger than the others. The above mentioned items were assumed to be more influenced by the price. 3. Farmer's intended cultivation areas of the major medidinal plants in 1996 were surveyed totals of 1.692 ha in Chonbuk. Among them, Aralia corolata was surveyed as the top totals of 426 ha. in area.

컴파일러 최적화를 위한 코드 스케쥴링

곽호영,한정현 제주대학교 1992 논문집 Vol.35 No.-

This paper deals with instruction scheduling, one area of compiler optimization. This assures the correctness of program execution during occuring the interlock and reduces the execution time in pipelined processor. DAG(Directed Acyclic Graph) is used to represent the problem and is proven to be pretty convinient to represent the various Kinds of serialization constraints. Gibbon's scheduling algorithm with O(n²) is introdued to schedule the instruction from DAG. Three heuristics and some assumptions are explained to prevent the problem to be O(n⁴). Furthermore, the impact on another phase in compiler is discused. Actually this scheduling algorithm assumes to perform the instruction scheduling after register allocation. More work relating to this area will be the relationship beween two phases-register allocation and code schduling.

황금(Scutellaria baicalensis) flavonoid의 대량 분리

곽호영,김동현,이윤형,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

황금의 뿌리를 물 또는 80% MeOH 로 추출하고 각각 농축하였다. MeOH 추출물은 물, EtOAc 및 n-BuOH로 분배 추출하였다. 얻어진 분획물에 대하여 silica gel column chromatography 하거나, 침전 및 여과과정을 반복하여 4종의 flavonoids 즉, baicalin, baicalein, wogonin 및 wogonoside를 높은 수율로 분리하였다. 분리한 화합물의 화학구조는 NMR을 포함한 물리화학적 data를 해석하여 동정하였다. The root of Scutellaria baicalensis was extracted with water or 80% aqueous MeOH and concentrated, respectively. The MeOH extract was partitioned between H_(2)O with organic solvents such as EtOAc and n-BuOH. The silica gel column chromatography or the precipitation method led to the isolation of four pure flavonoids, baicalin, baicalein, wogonin and wogonosides, with very high yield. The identification of the obtained flavonoids was carried out from the result of physico-chemical data including NMR.

부인과적 진단 복강경술에 대한 임상적 고찰

곽재환,박진완,황태영,김현호,이원기 동아대학교의과대학 1992 동아의대학술지 Vol.4 No.1

관상 동맥 질환에서 아포 E 지단백 유전자 다형성과 혈청 지질치와의 관계

곽선영,김성구,정호석,이유경,이광희,김철현,최태명,현민수,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and aims: The Apolipoprotein E is a ligand of both the protein component LDL receptor as well as the apo E LDL-Receptor related protein (LRP). It modulates the receptor binding of lipoproteins, with the apolipoprotein E found on cell surfaces as its component, thus serving an important role in the lipid metabolism by carrying out the intracellular transport of cholesterol in lipoproteins. The gene for apolipoprotein E is the product of three common genotypes as well as many more rare alleles. The common genotypes are ε2, ε3, and ε4, and are expressed in the three phenotype isoforms of E2, E3, and E4. In the event that E4 is the main component, a rise in the cholesterol level, as the result of down-regulation of the LDL receptor, is observed. Therefore, those samples with E4 genotypes are known to be in much higher risk of coronary artery disease than those with ε3/ε3, while those with ε2 are in low risk (with the exception of hypertiglyceremai Ⅲ). The aim of this study is to analyze in patients with ischemic heart disease the role of aplipoprotein E alleles in order to seek its correlation with coronary artery disease, as well as to seek whether the polymorphism of apo E produces any differences in the severity of coronary artery disease according to plasma lipid levels. Methods: The subjects for study were 273 patients admitted to the Internal Cardiology Division of the Soonchunhyang University Hospital form December 1998 to February 1999. The subjects were divided into the two groups of which one was ischemic heart disease (IHD) experiment group totaling 105 (avg.60.1 years of age, male/female ratio = 69/36) and the control group totaling 168 (avg. 59.7 years of age, male/female = 73:95). The coronary angiogram was given to 127 subjects, and of this total, 94 have developed significant stenosis in the coronary artery. The stages of the analyzing of the apo E phenotype was first, the separation of DNA from the blood samples, subjecting it to the PCR from with 228 base pairs of expanded products were obtained. The band was determined by means of the reverse hybridization principle on the nitrocellulose strip. Results: From the 105 patients the distributions of apo E phenotypes were as follows: ε3/2(5.7%), ε4/2(1.9%), ε3/3(70%), ε4/3(20%), ε4/4(1.9%). The relative frequencies of each allele are as follow: ε2 (0.038), ε3 (0.833), ε4 (0.128). The results show as follows: ⅰ) The IHD experiment group to have a higher occurrence of ε4/3 phenotypes as well as ε4 alleles than the control group. ⅱ) Both the control group and IHD group showed the largest distribution of ε3/3 for phenotypes, and ε4 for alleles. ⅲ) The IHD group showed less ε2/3 phenotypes as well as significantly less allele frequency of ε3 in comparison to the control group. ⅳ) the IHD group showed a much lower level of HDL in comparison to the control group, while the LDL was significantly higher; samples including the apo ε2 showed a significantly higher level of HDL than those without. Among the control group, samples including apo ε2 showed a significantly higher level of TG (triglyceride) than samples without. No significant difference was found between the experiment apo ε4 sample and the control plasma lipid sample. ⅴ) No significant correlation was found between an apo E polynorphism and the number of involved arteries of a coronary angiogram. Conclusion: Between the experiment IHD group and control group were found differences in the frequency of alleles. The polymorphism of apo E alleles may contribute as a risk factor to the development of heart disease by involving itself in the metabolism and modulation of plasma lipids.

시상부위에 발생한 단일성 뇌농양 : 증례 보고 및 문헌고찰 Report of Two Cases and Review of the Literature

곽호신,김동규,이상형,정희원,이현구,김현집,한대희 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.12

The authors present two cases of solitary thalamic abscess which were intially diagnosed as malignant thalamic glioma. After treatment with steroids and osmotic agents, the patients' clinical conditions improved after two to three days. However, sudden deterioration of consciousness level developed and the CT scan showed acute hydrocephalus with increment of thalamic low-density lesions. We performed extraventricular drainage and obtained CSF containing numerous inflammatory cells. This suggested the possibility that the lesion was a pyogenic abscess. which progressed to ventriculitis, rather than a malignant glioma. Stereotactic aspiration of the lesion confirmed thalamic abscess. These findings and review of previous reports of thalamic abscess suggested that early diagnosis and stereotactic drainage of the thalamic abscess is of primary importance.

全北地方의 藥用 資源植物 分布

郭晙洙,金昌洙,宋英恩,金柱,朴珍華,韓宗鉉,宋昊俊 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

국민 소득이 늘어나고 건강에 대한 관심이 높아지면서 한약재에 대한 수요가 증가하게 되고 WTO체제의 출범과 함께 선진각국의 유전자원 확보전이 치열해지면서 국내에서도 약용자원식물의 분포상황 및 이들 유전자원 식물의 보전이 중요한 과제로 대두되고 있다. 따라서 전북지방의 약초자원식물의 분포상황 및 이들 유전자원식물의 보전이 중요한 과제로 대두되고 있다. 따라서 전북지방의 약초자원식물의 분포현황을 조사하여 그에 대한 보전대책 수립 및 연구의 기초자료를 얻고자 수행한 본연구의 주요결과는 다음과 같다. 1. 전북지방에 분포하는 약용 자원식물의 목록을 작성한 결과 총 40목 129과에 걸쳐 762종류의 식물이 정리되었다. 2. 이들 분포식물중 항암효과가 보고된 약용식물은 36과 74종류였다. 3. 지역적으로는 덕유산지역에 가장 다양한 식물이 반포하고 다음으로는 남원, 변산반도, 모악산, 무주군, 완주고산, 내장산, 장안산, 선운산, 비안도, 적상산 등의 순으로 나타났다. 4. 식물군별로는 쌍자엽식물강의 이판화군과 합판화군이 가장 다양하였고, 각 목별 과(科) 수는 통화식물목이 10개 과로서 가장 많았으며, 각 목별 종류 수에 있어서는 장미목이 99종으로 가장 많았고, 초롱꽃목(77종), 통화식물목(67종), 백합목(53종), 미나리아재비목(43종), 산형화목(39종) 등이 비교적 많은 종들을 포함하는 것으로 분석되었다. 5. 각 과별 종수 분석에서는 국화과가 70종으로 가장 많았고, 장미과(46종), 콩과(40종), 백합과(40종)등으로 나타나 이들 4개과의 합계 종수가 196종류로서 전체의 25.72%를 차지하였다. 그 다음으로 산형과(27종), 미나리아재비과(26종), 꿀풀과(25종), 벼과(22종), 마디풀과(19종), 현삼과(15종), 석죽과(12종), 제비꽃과(11종), 난초과(11종) 등이 비교적 많은 종수를 가지는 과(科)들이었다. With the improving of the people's standard of life and the heightening of the concerns of health, the demands of medicinal plants are more rapidly increase. Also, with the inauguration of World Trade Organization, and acceleration of openness in domestic markets, the procurement competitions of gene pools in medicinal plants are more intense among the countries. With these trends of the world, an acute subjects are coming up for us such as distribution status quo and preservation of medicinal plants in domestic area. Therefore this paper surveyed the distribution status quo of medicinal plants in Chonbuk province, arranged the catalogue and analyzed the data by plants and regions. The major results are as follows; 1. The total amounts of 762 species, 129 families, 40 orders of medicinal plants distributed in Chonbuk province. 2. The number of medicinal plants which are assumed to contain antineoplastic agents totals 36 families and 74 species. 3. In distribution of medicinal plants by region, the most diverse flora showed in Mt. Dukyoo followed by circumferences of Namwon, Byun-san, Mt. Moak, Muju, Dosan, Mt. Naejang, Mt. Jangan, Mt. Seon-woon, Beeando island and Mt. Juksang etc. in Chonbuk province.

冷凍冷藏倉庫 前室部 侵氣防止裝置의 適用에 關한 硏究

석호태,곽현철,송승영,송진규,이영욱 대한건축학회 2003 대한건축학회논문집 Vol.19 No.4

This study aims to evaluate the economic performance of infiltration prevention devices for the refrigerated warehouse. It is showed that the cooling loads of refrigerated warehouses is changed by assembling of infiltration prevention devices in a previous study. Space loads and cooling energy costs according to the types of the infiltration prevention devices are analyzed by the DOE-2.1E simulation. Based on the Life Cycle Costs considered the costs of initial construction, repair·replace, cooling energy and operating energy, the probability of reducing energy consumption and application of infiltration prevent devices in vestibule of refrigerated warehouse is founded.