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최혁재,이우정,박성환,송보완,김동현,김남재 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-
In this study, We carried out the preparing standardization and regulation of processed Glycyrrhizae Radix(PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dray on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increase as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA obtained form heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGR in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on CCl_(1)-intoxicated rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was 150℃ for 80min.
박성환,이우정,최혁재,송보완,김동현,김남재 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-
We have studied the physico-chemical change and pharmacological transformation of traditional herbal medicines with processing. Evodia fruit (EF) has processed for the purpose of detoxification and reduction of its bitter taste. There are various methods of processing EF in Oriental medicinal references. Among them, we processed EF according to the method of Sang-han theory, the most famous medicinal reference. We processed EF with washing in hot water, and then dried. Processed EFs(PEFs) were prepared according to the above procedure through repetition of 1,3,5 and 7 times respectively. The contents of weight loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, alkaloids and limonin in non-processed EF (NPEF) and PEFs were examined. The weight loss, and contents of water extract and dilute ethanol extract in PEF showed decrease in proportion to increase of washing time, while the contents of ether extract, total ash and acid insoluble ash showed little change as compared with those of NPEF. And the content of evodiamine and rutaecarpine was not changed distinctly. However, the content of limonin decreased in the final processed material in proportion to increase of washing times with hot water. And the intense of bitter taste in PEF was also reduced. These results were ascribed to the flowing-out of the water-soluble portion. The biological activities of NPEF and PEF were also investigated. In the test of DPPH scavenging effect, xanthine oxidase inhibition effect and TBA-Rs effect, PEF was more effective than NPEF in vitro. Also, both NPEF and PEF showed potent analgesic and anti-inflammatory effects against in mice. Especially, PEF by 3 times washing with hot water was more effective than other PEFs. From these results, it is considered that PEF should be prepared by 3 times washing with hot water.
Crystal structure of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Thermus caldophilus
Kim, Mun-Kyoung,Cho, Min Kwan,Song, Hye-Eun,Kim, Dooil,Park, Bo-Hyu,Lee, Jun Hyuck,Kang, Gil Bu,Kim, Sung Hyun,Im, Young Jun,Lee, Dae-Sil,Eom, Soo Hyun Wiley Subscription Services, Inc., A Wiley Company 2007 Proteins Vol.66 No.3
Kim, Bo-Hyun,Jung, Ji-Ung,Ko, Ki-Sung,Kim, Won-Sin,Kim, Sun-Mi,Ryu, Jae-Sung,Jin, Jung-Woo,Yang, Hyo-Jung,Kim, Ji-Su,Kwon, Hyuck-Chan,Nam, Sang-Yoon,Kwak, Dong-Hoon,Park, Yong-Il,Koo, Deog-Bon,Choo, Y 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.1
Gangliosides are a family of sialic acid-containing glycosphingolipids that are abundant in neurons and have a variety of functions in developing and mature tissues. We examined the expression of ganglioside GT1b in the embryonic preimplantation stage after freezing and thawing processes to determine the regulatory roles of ganglioside GT1b in early embryonic development. ICR mouse embryos at the two-cell stage obtained by flushing the oviducts were frozen by two cryopreservation procedures, slow freezing using a programmable freezer or vitrification by direct plunging into liquid nitrogen. Slow freezing was conducted with equilibration in 1.5 M 1,2-propanediol or 5% equilibration glycerol. Vitrification was applied with a 10-15 min equilibration in 7.5% ethylene glycol (EG), 7.5% dimethylsulfoxide (DMSO), and 30 sec in a solution of 15% EG, 15% EMSO and 0.5 M sucrose. Immediately after thawing, the survival rate of the embryos was assessed by their morphology and ability to develop to blastocysts in culture. The survival rate of vitrified and thawed embryos (92%) was significantly higher than that of slow frozen and thawed embryos (76%) (P<0.05). A tendency of higher blastocyst rate was found in the vitrified and thawed embryos compared to that of the slow frozen and thawed embryos. Confocal immunofluorescence staining confirmed that surviving embryos expressed ganglioside GT1b, with the strongest expression at the compacted eight-cell or later stage embryos. Ganglioside GT1b was not observed in the TUNEL-positive, apoptotic embryos, suggesting that cryopreservation had induced DNA breaks in them. These results suggest that ganglioside GT1b may play an important role in embryo survival or development.
Kim, Bo-Kyung,Lee, Bo-Hwa,Lee, Yoo-Jung,Jin, Il-Hyuck,Chung, Chung-Han,Lee, Jin-Woo Elsevier 2009 Enzyme and microbial technology Vol.44 No.6
<P><B>Abstract</B></P><P>The microorganism hydrolyzing carboxymethylcellulose (CMC) was isolated from seawater, identified as <I>Bacillus subtilis</I> subsp. <I>subtilis</I> by analyses of 16S rDNA and partial sequences of the <I>gyr</I>A gene, and named as <I>B. subtilis</I> subsp. <I>subtilis</I> A-53. The molecular weight of the purified carboxymethylcellulase (CMCase) was estimated to be about 56kDa with the analysis of SDS-PAGE. The purified CMCase hydrolyzed carboxymethylcellulose (CMC), cellobiose, filter paper, and xylan, but not avicel, cellulose, and <I>p</I>-nitrophenyl-β-<SMALL>D</SMALL>-glucospyranoside (PNPG). Optimal temperature and pH for the CMCase activity were determined to be 50°C and 6.5, respectively. More than 70% of original CMCase activity was maintained at relative low temperatures ranging from 20 to 40°C after 24h incubation at 50°C. The CMCase activity was enhanced by EDTA and some metal ions in order of EDTA, K<SUP>+</SUP>, Ni<SUP>2+</SUP>, Sr<SUP>2+</SUP>, Pb<SUP>2+</SUP>, and Mn<SUP>2+</SUP>, but inhibited by Co<SUP>2+</SUP> and Hg<SUP>2+</SUP>.</P>
Bo-Hyun Kim,Ji-Ung Jung,고기성,Won-Sin Kim,Sun-Mi Kim,유재성,Jung-Woo Jin,Hyo-Jung Yang,Ji-Su Kim,Hyuck-Chan Kwon,남상윤,Dong-Hoon Kwak,박용일,Deog-Bon Koo,추영국 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.1
Gangliosides are a family of sialic acid-containing glycosphingolipids that are abundant in neurons and have a variety of functions in developing and mature tissues. We examined the expression of ganglioside GT1b in the embryonic preimplantation stage after freezing and thawing processes to determine the regulatory roles of ganglioside GT1b in early embryonic development. ICR mouse embryos at the two-cell stage obtained by flushing the oviducts were frozen by two cryopreservation procedures, slow freezing using a programmable freezer or vitrification by direct plunging into liquid nitrogen. Slow freezing was conducted with equilibration in 1.5 M 1,2-propanediol or 5% equilibration glycerol. Vitrification was applied with a 10-15 min equilibration in 7.5% ethylene glycol (EG), 7.5% dimethylsulfoxide (DMSO), and 30 sec in a solution of 15% EG, 15% DMSO and 0.5 M sucrose. Immediately after thawing, the survival rate of the embryos was assessed by their morphology and ability to develop to blastocysts in culture. The survival rate of vitrified and thawed embryos (92%) was significantly higher than that of slow frozen and thawed embryos (76%) (P<0.05). A tendency of higher blastocyst rate was found in the vitrified and thawed embryos compared to that of the slow frozen and thawed embryos. Confocal immunofluorescence staining confirmed that surviving embryos expressed ganglioside GT1b, with the strongest expression at the compacted eight-cell or later stage embryos. Ganglioside GT1b was not observed in the TUNEL-positive, apoptotic embryos, suggesting that cryopreservation had induced DNA breaks in them. These results suggest that ganglioside GT1b may play an important role in embryo survival or development.
( Byoung Hyuck Kim ),( Eui Kyu Chie ),( Kyu Bo Kim ),( Jin Young Jang ),( Sun Whe Kim ),( Sae Won Han ),( Do Youn Oh ),( Seock Ah Im ),( Tae You Kim ),( Yung Jue Bang ),( Sung W Ha ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: To date, there is a paucity of information regarding optimal radiation dose after microscopic positive (R1) resection in patients with extrahepatic bile duct cancer. This study was conducted to evaluate the impact of radiation dose escalation for R1 resection margin (RM). Methods: A retrospective review was performed on 251 patients who underwent curative resection followed by adjuvant chemoradiotherapy between 1995 and 2009. Eighty-six patients had R1 RM which included invasive carcinoma (n=63) and carcinoma in situ/high-grade dysplasia (n=23). Among them, 54 patients received radiation dose < 54 Gy (range, 40-50.4 Gy) and 32 patients received = 54 Gy (range, 54-56 Gy). Concomitant fi uoropyrimidine-based chemotherapy was administered. Acute and late gastrointestinal (GI) toxicity were evaluated using Radiation Therapy Oncology Group criteria. The median follow-up duration was 27 months for all patients and 92 months for survivors. Results: Radiation dose = 54 Gy was associated with improved locoregional control (LRC) (5yr rate, 73.8% vs. 47.1%, p = 0.038), but not disease-free survival (5yr rate, 43.4% vs. 32.6%, p = 0.427) and overall survival (5yr rate, 40.6% vs. 29.6%, p = 0.393). In multivariate analysis for LRC, R1 with invasive carcinoma (HR 3.31, 95% CI 1.11-9.85, p = 0.032) and radiation dose < 54 Gy (HR 3.09, 95% CI 1.14-8.39, p=0.027) were independent adverse prognostic factors. Between the two dose groups, there was no signifi cant difference in acute GI toxicity =grade 2 and in late GI toxicity =grade 3. Conclusions: The current study demonstrated that radiation dose = 54 Gy was associated with improved LRC in patients with R1 RM and also well tolerated. Therefore, dose escalation could be considered for R1 RM. Prospective study is needed to verify these results.