http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
$2{\beta}$, $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid처리에 의한 인간 간암세포주 HepG2의 apoptosis 유도
유기현,이종민,황보전,송명종,양혜정,백남인,김성훈,김대근,권병목,박미현,정인식,Yoo, Ki-Hyun,Lee, Jong-Min,HwangBo, Jeon,Song, Myoung-Chong,Yang, Hye-Joung,Baek, Nam-In,Kim, Soung-Hoon,Kim, Dae-Keun,Kwon, Byoung-Mok,Park, Mi-Hyun,Chung, 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.4
Triterpenoid를 포함하고 있는 $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid를 애기마름으로부터 분리하였다. 이것은 pentacyclic triterpenes의 공통 구조를 가지며 amyrin ursolic acid 그룹에 속해 있다. 본 연구에서는 이 화합물의 독성 영향을 인간 간암 세포주인 HepG2에서 조사하였다. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid는 처리한 양에 비례하여 HepG2 세포주에서 독성을 보였다. 그리고 Confocal microscopy 결과는 $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid를 HepG2 세포에 처리한 시간에 비례하여 녹색 형광의 증가를 보여주었다. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid는 또한 HepG2 세포의 sub-G1 cell population 뿐만 아니라 DNA 분절(fragmentation) 현상의 증가를 보여 주었다. 이러한 결과는 $22{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid가 HepG2 세포에서 apoptosis를 통한 세포 사멸 유도를 의미한다. [ $2{\beta},\;3{\alpha}$ ], 23-trihydroxyrus-12-ene-28-oic acid was isolated from Trapa pseudoincisa S. et Z. It has a common structure of pentacyclic triterpenes and belongs to the amyrin ursolic acid group. The cytotoxic effect of this compound was investigated in human hepatoma cell line HepG2. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid showed dose-dependent cytotoxicity in HepG2 cells. Confocal microscopy data showed that green fluorescence was increased in $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid treated-HepG2 cells in a time-dependent manner. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid also increased the sub-G1 cell population of HepG2 cells as well as ladder-like DNA fragmentation. Taken together, our results indicate that $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid induced apoptosis in HepG2 cells.
장경화,박종화,김도형,정하영,황보전,이현호,이희영,손동화,김원용,정인식,Chang, Kyung Hwa,Park, Jong-Hwa,Kim, Do Hyung,Chung, Ha Young,HwangBo, Jeon,Lee, Hyun Ho,Lee, Hee-Young,Shon, Dong-Hwa,Kim, Wonyong,Chung, In Sik The Korean Society for Biotechnology and Bioengine 2012 KSBB Journal Vol.5 No.3
Dimethyl sulfoxide (DMSO) increased the intracellular calcium ion concentration in stably transfected Drosophila melanogaster S2 cells expressing recombinant cyclooxygenase 1 (COX-1). DMSO did not increase the Drosophila NOS (dNOS) transcript level in calcium chelator-treated cells. Expression of recombinant COX-1 due to DMSO was diminished in cells treated with calcium chelators or channel blockers. Our results indicate that an increased intracellular calcium ion concentration due to DMSO is associated with up-regulation of the dNOS gene, leading to enhanced expression of COX-1.
미생물,발효,생물공학 : 2β, 3α, 23-trihydroxyrus-12-ene-28-oic acid 처리에 의한 인간 간암세포주 HepG2의 apoptosis 유도
유기현 ( Ki Hyun Yoo ),이종민 ( Jong Min Lee ),황보전 ( Jeon Hwangbo ),송명종 ( Myoung Chong Song ),양혜정 ( Hye Joung Yang ),백남인 ( Nam In Baek ),김성훈 ( Soung Hoon Kim ),김대근 ( Dae Keun Kim ),권병목 ( Byoung Mok Kwon ),박 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.4