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주반멘,장유선,이은주,이은실,강종성 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-
Lonicerae Folium et Caulis is a commonly used anti-inflammatory herbal drug. The therapeuticeffectiveness of this drug depends significantly on the geographical origin, hence, the standardization and the geographical authenticity verification using the chemical characteristics would be very important. In the present study, an HPLC method for simple and effective analysis of four main components like coniferin, loganic acid, sweroside and loganin from the Lonicerae Folium et Caulis sample was developed and validated. Four main components were base line separated on a Optimapak C18 column with linear gradient of mobile phase A(10% methanol containing 0.1% formic acid) and B(90% methanol containing 0.1% formic acid) for 0 min 0%B, 15 min 30%B, 25 min 70%B, 30 min 70%B, 35 min 100%B. The flow rate was 1.0 mL/min and detection was carried out at 254 nm. Methylparaben was used as internal standard. Pattern recognition analysis using similarity index and principal components analysis revealed that Korean samples could not discriminated from Chinese samples because the contents of the marker components of samples were very similar. This method was validated according to ICH Guidelines and the method was found to be simple, accurate, precise, economical, and robust.
키랄컬럼 쌍을 이용한 레보도파 제제 중의 l-도파와 d-도파의 HPLC 분석
주홍매,오은기,진건파,주반멘,이은주,이은실,강종성 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-
Levodopa preparations are used for the treatment of Parkinson's disease, since it is capable of crossing the protective blood-brain barrier (BBB), whereas dopamine itself cannot. d-Dopa could be often contained to a pharmaceutical levodopa preparations as impurity, however, the Korean Pharmacopeia doesn't regulate the amount of d-dopa in levodopa preparations. A new, precise and simple HPLC method for determination of l-dopa and d-dopa from levodopa formulation was developed using a pair of chiral columns, SCA and RCA, for the purpose of quality control. The selected mobile phase was composed of methanol and water (80 : 20) containing 0.01% phosphoric acid. The flow rate was 1.0 mL/min and detection wavelength was 210 nm. The intraday precision of l-dopa and d-dopa analysed by developed method were 2.71~8.18% and 2.94~6.03%, respectively. Both dopa enantiomers were separated by SCA and RCA column, a pair of chiral columns. However, RCA column was useful for the analysis of l-dopa, while SCA column for d-dopa. The method was validated according to ICH Guidelines and the method was found to be simple, accurate, precise, economical and robust.
다중요인 분석에 의한 전탕용수에 따른 현호색 성분의 추출효과 비교
김태진,서은영,장유선,김상혁,김대현,주반멘,김동희,강종성 한국생약학회 2012 생약학회지 Vol.43 No.1
The characteristics of decoction water are important for the extraction of herbal drugs. In this study, the relationship between the characteristics of water and extraction efficiency of bioactive compounds from Corydalis tuber was investigated. Six different types of decoction water, including three types of mineral water from Jeju Island and three types of bottled water sold in market, were used as decoction water for the extraction of bioactive compounds from Corydalis tuber. The contents of minerals in six types of water were analyzed by ICP-AES and ICP-MS; the contents of extracted bioactive compounds were analyzed by HPLC. Multivariate analysis of variance was used to analyze the differences in total extraction and bioactive compounds extracted with six types of water. Multiple factor analysis was used to analyze the relationship among the pH, content of mineral, anion, total extractions and content of bioactive components as factors. There was a significant difference among the six types of water in the total extracts and the bioactive compounds. The results proved that the content of extracted compounds from Corydalis tuber was influenced by the types of decoction water.