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Disulfiram에 의한 type IV collagenase 저해효과
신두일,박재복,박관규,조창호,오훈규,최창혁,조현지,장영채,Sin, Doo-Il,Park, Jae-Bok,Park, Kwan-Kyu,Cho, Chang-Ho,Oh, Hoon-Kyu,Choi, Chang-Hyuk,Cho, Hyun-Ji,Chang, Young-Chae 한국생명과학회 2006 생명과학회지 Vol.16 No.6
기질의 침윤과 전이를 특징으로 하는 악성종양 세포는 세포외 기질이나 기저막에 의존적으로 작용한다. 세포외 기질을 분해하는 효소인 matrix metalloproteinase (MMP) 계들의 발현 및 활성증가는 대부분의 악성종양세포에서 전이와 침윤을 촉진시킨다. MMP family 가운데 특히 type IV collagenase 활성을 지닌 MMP-2와 MMP-9은 세포외기질의 중요한 구성분인 collagen, fibronectin을 분해하는 특성을 가지며 암 전이를 용이하게 하는 주요한 효소로 잘 알려져 있다. 본 연구에서는 항암후보물질인 disulfiram이 골 육종 (U20S), 신장암 (Caki-1) 및 자궁암 (Caski) 세포에서 MMP-2와 MMP-9의 효소활성 및 발현억제에 대해 조사하였다. MTT assay를 이용하여 disulfiram에 대한 암세포 viability 실험에서는 disulfiram이 암세포의 viability를 저해하였다. 또한 zymography, western blot 및 RT-PCR 등을 이용한 type IV collagenase의 활성 및 발현 실험에서 disulfiram은 type IV collagenase의 활성을 비롯하여 단백질 및 mRNA 발현을 억제시키는 것을 확인하였다. 따라서 disulfiram이 MMP-2와 MMP-9의 활성 및 발현 억제 기전을 통하여 골 육종, 신장암 및 자궁경부암 세포의 작용을 억제한다는 연구 결과는 disulfiram이 각종 악성종양의 침윤과 전이를 억제 또는 방지하기 위한 치료물질로서 임상에서 활용할 수 있는 가능성을 보여준다. The cancer cells, characterized by local invasion and distant metastasis, are very dependant on extracellular matrix. The expression of matrix metalloproteinases (MMPs) has been implicated in the invasion and metastasis of cancer cells. Among the human MMPs, matirx metalloproteinase-2 (MMP-2) and matrix metalloproteinse-9 (MMP-9) are key enzymes that degrade type IV collagen of the matrix. Here, we studied the effect of disulfiram, an anti-tumor compound, on the suppression of the tumor invasion and the activity of MMP-2, MMP-9 in human osteosarcoma cells (U2OS). Disulfiram had the type IV collagenase inhibitory activity, the effect of inhibition of gene and protein expression, and these inhibitions were responsible for blocking invasion through cell mediated and non-cell mediated pathways. In conclusion, disulfiram inhibited expression of MMP-2 and MMP-9, and regulated the invasion of U2OS, Caki-1 and Caski. These observations raise the possibility of clinical therapeutic applications for disulfiram used as a potential inhibitor of cancer invasion.
Giant Right Atrial Thrombi Formation Associated with Hemodialysis Catheter Placement in a Dog
신두리,양혁주,김혜영,송근호,서경원 한국임상수의학회 2017 한국임상수의학회지 Vol.34 No.6
A Cocker spaniel (7-year-old, female) was presented with one week of anorexia, halitosis, oral ulcer, intermittent vomiting, acute weight loss and 3-day history of oliguria. The patient was diagnosed with acute on chronic kidney disease and pancreatitis. Hemodialysis was continued three times a week (total 7 sessions) with improved clinical signs and kidney panel, but presented with another episode of abdominal distention, respiratory distress, and large bowel diarrhea. Echocardiography revealed nearly totally occlusive thrombus in the cranial vena cava, as well as a right atrial mass of approximately 2 cm × 1 cm. The patient was treated with catheter removal, thrombolysis and anticoagulation therapy with recombinant tissue plasminogen activator. During anticoagulation therapy, size of atrial thrombus was not changed and heart function was not improved after treatment. Since clinical signs were deteriorated, the patient was euthanized by owner’s request. Catheter-related giant thrombus in right atrium is a rare complication and treatment guideline for atrial thrombus does not exist. This case is first report of hemodialysis catheter-induced thrombus in Korea.
신두일(Doo-Il Shin),신동주(Dong-Ju Shin),최성(Sung Choi),박지홍(Jee-Hong Park) 대한정형외과학회 2013 대한정형외과학회지 Vol.48 No.6
견관절 전방 탈구는 인체의 모든 관절 탈구 중 가장 흔하다. 여러 방법의 정복술이 소개되고 있으나 상완신경총 손상, 액와신경 손상 및 상완골 골절 등의 합병증이 발생할 수 있다. 또한 정복을 위해 사용되는 약물로 인한 심혈관계 부작용 및 호흡기계 억제 등에 대한 우려도 있다. 저자들은 이러한 합병증의 발생을 최소화하고 정복 성공률을 높이기 위한 새로운 방법을 고안하여 정복을 시행하였으며 23예 모두에서 합병증 없이 성공적인 정복을 얻었기에 이를 견관절 전방 탈구 정복의 추가적인 방법으로 제시하고자 한다. The most common form of joint dislocation is anterior shoulder dislocation. There are a number of available techniques to reduce, but which have a possibility to be happened many kinds of complications like brachial plexus injury, axillary nerve injury and fracture of the humerus. In addition, the drugs we use for the reduction have potential side effects such as cardiovascular complications and respiratory arrest. We are trying to introduce new reduction technique aimed at minimizing the complications and increasing the success rate, which was used in 23 cases and all of them were successfully reduced without complications.
연구논문 : 서울지역 여성소비자의 1회 제공량 기준 영양표시의 이용실태
신두지 ( Doo Jee Shin ),정경완 ( Kyoung Wan Jung ),이귀주 ( Gui Chu Lee ),권광일 ( Kwang Il Kwon ),김지영 ( Jee Young Kim ),김종욱 ( John Wook Kim ),문귀임 ( Gui Im Moon ),박혜경 ( Hye Kyung Park ),조윤미 ( Yoon Mi Cho ),김유경 ( 한국식생활문화학회 2010 韓國食生活文化學會誌 Vol.25 No.6
This study examined the understanding and use of information on nutrition labels based on one serving size among female consumers above the age of 20 in Seoul area. According to the survey, 69.9% of respondents were aware of the current system of nutrition labeling based on one serving size, and 51.8% of the respondents expressed their dissatisfaction with the system because the nutrition labels were difficult to understand or appeared unreliable. The nutrition label literacy of the consumers varied with respect to different packaging units. The respondents were likely to be less accurate in calculating the expected caloric intake when only portions of a multi-serving package were used. Initially 69.0% of respondents reported that they had read the nutrition label before purchasing a product but 91.9% of respondents said that they would check the label after learning how to read the label properly. It is very important to make consumers aware that the labels are very reliable sources of nutrition information. A public education campaign on the use of nutrition labels should focus on developing the consumers` ability and skills in using the label information when choosing foods.