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파킨슨병 모델 동물에서 GDNF 아데노바이러스 벡터를 이용한 유전자치료
성기한(Ki-Han Sung),윤인규(In-Gyu Yoon),정현실(Hyun-Sil Jung),김을선(Eul-Seon Kim),김현주(Hyun-Joo Kim),김양호(Yang-Ho Kim),윤 식(Sik Yoon),김봉선(Bong-Seon Kim),김재봉(Jae-Bong Kim),백선용(Sun-Yong Baek) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.6
6-OHDA를 이용하여 실험적으로 유발한 파킨슨병 모델 동물을 대상으로 유전자 치료 목적으로 GDNF 유전자를 포함 하는 아데노바이러스 벡터(Ad-GDNF)를 주사하여 행동 변화와 줄무늬체와 흑색질의 tyrosine hydroxylase (TH) 면역반응양성의 도파민성 세포의 형태학적 변화를 연구하였다. 성숙한 수컷 Sprague-Dawley계 흰쥐의 줄무늬체에 6-OHDA를 주사하고, 1주 후에 apomorphine에 의한 회전 운동 양상 을 측정하여 분당 7회 이상 회전하는 동물은 파킨슨병 모델 동물에 해당하는 것으로 하였다. 이들 동물의 줄무늬체에 Ad-GDNF를 주사하고 1주 후에 apomorphine에 의한 행동 양상을 다시 측정하고, 이어서 심장 관류고정을 실시하여 형태 학적 변화를 관찰하였다. Ad-GDNF 대신에 LacZ 유전자를 포함하는 아데노바이러스 벡터(Ad-LacZ)를 주사한 것을 대조 군으로 이용하였다. 1. 파킨슨병 모델 동물의 줄무늬체에 Ad-GDNF를 주사한 동물에서 apomorphine에 의한 회전 운동 수가 유의하게 감소 하였다. 그러나 Ad-LacZ를 주사한 대조군에서는 회전 운동 수의 변화를 관찰할 수 없었다. 2. 회전 운동 수가 감소한 동물의 줄무늬체에 존재하는 TH 면역반응 양성의 축삭 종말은 대조군의 75% 수준으로 Ad- LacZ를 주사한 37%에 비하여 현저히 증가하여 TH 면역반응 양성 축삭종말의 퇴행성 변화가 방지되었다. 3. 흑색질의 TH 면역반응 양성 신경세포의 수는 Ad-GDNF를 주사한 군에서는 대조군의 82% 수준으로 Ad-LacZ를 주사한 군의 60% 수준에 비하여 유의하게 증가되어 있어서 6-OHDA에 의한 신경세포의 사멸이 방지되는 것을 알 수 있었다. 이상의 결과로 미루어 파킨슨병 모델 흰쥐의 줄무늬체에 Ad-GDNF를 한차례 주사하여 1주 후에 줄무늬체와 흑색질의 도파민성 세포의 사멸이 방지되는 것을 확인하였다. A recombinant adenoviral vector encoding human GDNF gene (Ad-GDNF) was developed to investigate the effect of GDNF gene in 6-OHDA-induced Parkinson’s disease rat. The changes of rotatory behavior and density of THimmunoreactive axon terminals and number of cell bodies were observed by the GDNF expression. Adult male Sprague-Dawley rats were used. Parkinson’s disease (PD) rat models were prepared by the injection of 6-OHDA into the striatum. After 1 week, the animals showing apomorphine-induced rotatory behavior above 7 turns/ min were defined as PD rat model. Ad-GDNF was injected into the striatum of animal model and tested the apomorphine- induced rotatory behavior at 1 week after injection. These animals were perfused with Zamboni fixative to investigate the morphological changes after rotatory behavior test. Instead of Ad-GDNF, Ad-LacZ injected PD rats were used as a control. The following results are obtained: 1. The apomorphine-induced rotational behavior was significantly reduced by the treatment of PD rat by the injection of Ad-GDNF. The Ad-LacZ injected PD rat showed no change in rotatory behavior. 2. The density of TH-ir axon terminals in the striatum was significantly increased by the Ad-GDNF injection (75% of normal side), but there was no change in the density by the Ad-LacZ injection (37% of normal side) compared to 6-OHDA lesioned striatum. This means the Ad-GDNF injection prevented the degenerative change of TH-ir axon terminals in the stritum of the PD rat. 3. The number of TH-ir cell body was significantly recovered by the Ad-GDNF (82% of normal side), but there was not recovered by the Ad-LacZ injection (60% of normal side) compared to 6-OHDA lesion. This means the retrogradely transported Ad-GDNF induced the TH expression in the dopaminergic neurons of the substantia nigra. Gene therapy with Ad-GDNF prevented the degeneration of dopaminergic neurons and axon terminals in the 6- OHDA-induced PD rat.
생쥐 난자의 체외 성숙에 미치는 Nicotine의 영향
성기청,배인하,Sung, Ki-Cheong,Bae, In-Ha 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.1
Objective: The present study was done to clarify the effects of nicotine and nicotine tartrate on the mouse oocyte maturation in vitro. Methods: GV (germinal vesicle) oocytes were isolated from Graafian follicle of ovaries with sharp needles under a stereomicroscope from female mouse of ICR strain (4 weeks old). Collected oocytes were cultured for 17 hours at $37^{\circ}C$, 5% $CO_2$ in air and 100% humidified condition in incubator. New MHBS was the basic medium used in which nicotine, nicotine tartrate, and mecamylamine (antagonist of nicotinic acetylcholine receptor) were added depending on the experimental group. GV oocytes were cultured in one of these media. Results: Nicotine ($300{\mu}M{\sim}5mM$) had no effects on GVBD (germinal vesicle breakdown) compared to the control, but increasing concentration of nicotine led to an decrease in the first polar body formation. However, nicotine ($10{\sim}500{\mu}M$) induced GVBD in a dose-dependent manner of GV oocytes in a medium containing dbcAMP. Nicotine tartrate ($50{\mu}M{\sim}5mM$) had no effects on GVBD compared to the control but, increasing concentration of nicotine tartrate led to an decrease in the first polar body formation. Mecamylamine $10{\mu}M$ added to the medium containing nicotine ($300{\mu}M{\sim}5mM$) showed higher percentage of the first polar body formation compared to the nicotine ($300{\mu}M{\sim}5mM$) treatment group. Mecamylamine $10{\mu}M$ added to the medium containing nicotine tartrate ($50{\mu}M{\sim}5mM$) showed higher percentage of the first polar body formation compared to the nicotine tartrate ($50{\mu}M{\sim}5mM$) treatment group. Conclusion: The present study suggest that nicotine and nicotine tartrate have the harmful effects on the meiotic maturation of the mouse oocytes in vitro. However, mecamylamine block harmful effects of nicotine and nictine tartrate.
성기선,유인상,Sung, Ki-Sun,Yu, In-Sang 대한족부족관절학회 2014 대한족부족관절학회지 Vol.18 No.3
Acquired adult flatfoot is a deformity characterized by a decreased medial longitudinal arch and a hindfoot valgus with or without forefoot abduction. The etiologies of this deformity include posterior tibial tendon dysfunction, rheumatoid arthritis, trauma, Charcot's joint, neurologic deficit, and damage to the medial spring ligament complex or plantar fascia. Among these, posterior tibial tendon dysfunction is the most well-known cause. Although posterior tibial tendon dysfunction has been regarded as a synonym of acquired adult acquired flatfoot, failure of the ligaments supporting the arch can also result in progressive deformity even without a posterior tibial tendon problem. The authors describe the pathophysiology, diagnosis, and nonoperative treatment of acquired adult flatfoot, focusing on posterior tibial tendon dysfunction.
성기창(Sung Ki-Chang),강병근(Kang Byoung-Keun),박광재(Park Guang-Jae),김상운(Kim Sang-Woon),윤영삼(Yun Young-Sam),강태성(Kang Tae-Sung),김인영(Kim In-Young),이영환(Lee Young-Huan) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.9
The purpose of this study is improving the convenience of the disabled who use accommodation in recreational facilities. Rooms and accommodations are essentially requied facilities in recreational facilities. In order to derive the direction of improvement, it would be identified problems of the disabled who feel uncomfortable using accommodation and analyze features and space which rooms should have. Survey is limited professional recreation, general recreation, and national natural forest defined 『The law of tourism』, with accommodation facilities to be able to stay. One of professional recreation, two of general recreation, six of national natural forest were chosen. It is also proposed directions of system and servicies, spatial configuration, room information, the direction of evacuation plans of room facilities through tracking analysis about available rooms space, configuration analysis, and a flat space analysis, amenities of room facilities.
고반복율 인공위성 레이저추적을 위한 운영 소프트웨어 개발
성기평(Ki-Pyoung Sung),최은정(Eun-Jung Choi),임형철(Hyung-Chul Lim),정찬규(Chan-Gyu Jung),김인영(In-Yeong Kim),최재승(Jae-Seung Choi) 한국항공우주학회 2016 韓國航空宇宙學會誌 Vol.44 No.12
한국천문연구원은 인공위성 정밀궤도 결정, 우주측지 및 인공위성 자세역학 연구를 위해서 2kHz 반복율을 가지는 SLR 시스템을 운영하고 있다. 그러나 측지위성의 회전속도를 보다 정밀히 결정하고 거리 측정 정밀도 향상을 위해서 고반복율의 SLR 관측 데이터가 요구된다. 따라서 고반복율 시스템 구현을 위해 운영 소프트웨어 및 레인지 게이트 생성기를 개발하여 최대 10kHz 반복율로 레이저추적이 가능한 HSLR-10(High repetition-rate Satellite Laser Ranging-10kHz) 시스템으로 개선하였다. 본 연구에서는 10kHz 반복율을 가지는 HSLR-10 시스템의 운영 소프트웨어 개발 방법, 구성 및 검증 결과를 제시한다. Korea Astronomy and Space Science Institute (KASI) has been operating SLR (Satellite Laser Ranging) system with 2kHz repetition rate for satellite precise orbit and spin determination as well as space geodesy. But the SLR system was improved to be capable of laser ranging with high repetition rate, up to 10kHz by developing new operation software and novel range gate generator, called HSLR-10. The HSLR-10 will contribute to the accurate spin rate determination of geodetic satellites and geodetic research due to its largest repetition rate in the world. In this study, the development methodology and configuration of operation software are addressed, and its validation results are also presented.
프로그램 운영형태에 대응한 장애인복지관의 공간구성 방향
강병근(Kang Byoung-Keun),성기창(Seong Ki-Chang),박광재(Park Kwang-Jae),김인순(Kim In-Soon),윤영삼(Yun Young-Sam),김상운(Kim Sang-Woon),강태성(Kang Tae-Sung),노태헌(Noh Tae-Heon) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.2
The Rehabilitation Facilities for disabled people keeps going to grow with social changes that disabled people are dramatically increasing. Despite its growth health center newly established is organized by means of following the program and making up space being operated at current center rather than considering the relation between program and space being operated. However, future health center has to be made up not only for disabled people but also for maximum thinking of harmonization with a local resident. Now, this study suggests that we should have a thorough grasp and analyzation about program and making up space at current Rehabilitation Facilities and it is available to be rational for the future health center.
프레스 가공 공정을 이용한 가설구조물 제조에 관한 연구
박준홍(Joon-Hong Park),이재섭(Jae-Sup Lee),이성근(Sung-Keun Lee),김현수(Hyun-Soo Kim),성기인(Ki-In Sung) 한국기계가공학회 2007 한국기계가공학회지 Vol.6 No.4
A temporary structure is a framework of metal poles and planks, used as a working platform from which building repairs or construction can be carried out safety. The manufacturing process of the existing model consumes a many hour. So, it is modified manufacturing process. In this study, it is attempted to find out the shape of modified working platform considering manufacturing process and using Taguchi method, and it reduces production process. The design parameters are defined to discribe shape of model. As a result of the shape design, maximum Von-Mises stress and displacement considering a safety factor were satisfied with CTEA (Construction Temporary Equipment Association of Korea) standard.