삼릉(三稜) 추출물의 간세포 보호 및 미토콘드리아 보호 효과

서혜림,이주희,장미희,권영원,조일제,김광중,박숙자,김상찬,김영우,변성희,Seo, Hye-Lim,Lee, Ju-Hee,Jang, Mi-Hee,Kwon, Young-Won,Cho, Il-Je,Kim, Kwang-Joong,Park, Sook-Jahr,Kim, Sang-Chan,Kim, Young-Woo,Byun, Sung-Hui 대한한의학방제학회 2015 大韓韓醫學方劑學會誌 Vol.23 No.2

Objectives : Sparganii Rhizoma is frequently used in traditional herbal medicine for treatment of blood stasis, amenorrhea and functional dyspepsia and has been reported to exhibit anti-oxidant, anti-proliferation and anti-angiogenesis peoperties. In this study, we investigated the cytoprotective effect and underlying mechanism of Sparganii Rhizoma water extract (SRE) against oxidative stress-induced mitochondrial dysfunction and apoptosis in hepatocyte. Methods : To determine the effects of SRE on oxidative stress, we induced synergistic cytotoxicity by co-treatment of arachidonic acid (AA) and iron in the HepG2 cell, a human derived hepatocyte cell line. Results : Treatment of SRE increased relative cell viability and altered the expression levels of apoptosis-related proteins such as Bcl-xL, Bcl-2 and procaspase-3. And SRE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species production induced by AA+iron. In addition, SRE activated of AMP-activated protein kinase (AMPK), a potential target for cytoprotection, by increasing the phosphorylation of AMPKα at Thr-172. Morever, SRE increased phosphorylation of acetyl-CoA carboxylase, a direct downstream target of AMPK. Conclusion : These results indicated that SRE has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with AMPK pathway.

영상의학검사 일반촬영 분야의 촬영기법에 대한 분석

김정수(Jung-Su Kim),김정민(Jung-Min Kim),이영한(Young-Han Lee),서덕남(Deok-Nam Seo),최인석(In-Seok Choi),남소라(So-Ra Nam),윤용수(Yong-su Yoon),김현지(Hyun-Ji Kim),민혜림(Hye-Lim Min),허재(Jea Her),한성규(Seong-Gyu Han) 대한방사선과학회(구 대한방사선기술학회) 2014 방사선기술과학 Vol.37 No.3

2013년 국민건강보험심사평가원의 의료기관 데이터베이스에 따르면 1118개의 병.의원에 영상의학과가 개설되어있다. 이들 병원에는 CT, 투시촬영장치, 일반촬영장치와 같은 의료용 방사선 발생장치가 운영되고 있다. 이 중에서도 일반촬영장치는 가장 많은 병원에서 운영되고 있는 장비이다. 일반촬영장치의 경우 film-screen 장치에서 digital radiography 로 급격하게 변하고 있다. 하지만 그 촬영기법은 films-screen 기법 을 그대로 사용하고 있어 디지털 장치의 맞는 촬영기법의 개발을 위한 전반적인 실태 조사가 필요하다. 이에 본 연구에서는 국내 의료기관의 일반촬영기법에 관한 조사를 시행하여 실제 병원에서 사용하고 있는 일반촬 영기법의 기술적 항목에 대한 현황을 파악하여 보았다. 본 연구에서는 의료기관에서 일반적으로 사용되는 일 반촬영기법 26개에 대한 촬영기법의 전국 단위 조사에서 흉부, 두부, 척추, 골반에 해당하는 검사에 대한 분석을 시행하였다. According to database of medical institutions of health insurance review & assessment service in 2013, 1118 hospitals and clinics have department of radiology in Korea. And there are CT, fluoroscopic and general radiographic equipment in those hospitals. Above all, general radiographic equipment is the most commonly used in the radiology department. And most of the general radiographic equipment are chang-ing the digital radiography system from the film-screen types of the radiography system nowadays. However, most of the digital radiography department are used the film-screen types of the radiography system. Therefore, in this study, we confirmed present conditions of technical items for general radiog-raphy used in hospital and research on general radiographic techniques in domestic medical institutions. We analyzed 26 radiography projection method including chest, skull, spine and pelvis which are gen-erally used in the radiography department.

여성의 가족가치관과 결혼 및 출산의향

임병인 ( Lim Byung-in ),서혜림 ( Seo Hye-rim ) 한국보건사회연구원 2021 保健社會硏究 Vol.41 No.2

본 연구에서는 미혼 여성의 결혼의향, 기혼여성의 출산, 추가출산의향에 결혼, 자녀, 성역할 가치관이 미치는 효과를 2015년과 2018년의 『전국 출산력 및 가족보건, 복지실태조사』를 사용하여 로지스틱 회귀분석으로 실증하였다. 분석결과, 첫째, 2015년 이후 3년 동안 미혼 여성의 결혼의향과 자녀가 있는 기혼여성의 추가출산의향이 모두 통계적으로 유의하게 낮아졌다. 둘째, 미혼 여성과 자녀가 없는 기혼여성은 취업한 경우 결혼, 출산의향에 긍정적이고, 자녀가 있는 기혼여성은 결혼 당시 자가에 거주할 경우 추가출산의향이 더 높았다. 이는 첫 자녀출산 결정에는 취업여부, 첫 자녀 이후 추가출산 결정에는 주거상황이 영향을 줄 수 있음을 보여준다. 셋째, 미혼 여성은 전통적 가족가치관에 긍정적일수록 결혼의향이 높았지만, 기혼여성의 출산의향에 가치관이 주는 방향은 일관되지 않고 혼재되어 있다. 넷째, 국가의 결혼, 출산, 육아에 관한 지원정책이 필요하다고 생각하는 미혼 여성과 기혼여성이 증가하였고, 더 나아가 국가의 적극적인 지원정책이 결혼의향과 추가 출산의향을 높이는 것으로 나타났다. 이상에서 미혼여성과 자녀가 없는 기혼여성에게는 고용안정에 대한 실효성 있는 정책, 자녀가 있는 기혼여성에게는 현재의 주거안정정책을 보완, 강화할 필요가 있다는 정책적 시사점을 도출할 수 있다. We examined if the values women have of marriage, childbirth, and gender role affect their intention to marry and to have children. For our analysis, we used logistic regression estimation and multiple regression on data from the 『National Survey on Fertility and Family Health and Welfare』 of 2015 and 2018. Empirical findings are as follows: first, both unmarried women’s intention to marry and married women’s intention to have an additional childbirth has declined to a statistically significant extent since 2015. Second, in both unmarried women and married women without children, being in employment positively affected the intention both to marry and to have a child. Among married women with children, being in employment did not affect the intention to have another child. Having a home of one’s own was associated with increased intention to have a child. Third, the more positive unmarried women were about family values in the traditional sense, the higher their intention to get married. Married women, however, appeared to have inconsistent and mixed values affecting their intention to give birth. Fourth, an increasing proportion of unmarried or married women were found to think that promoting marriage and childbirth should require the increased support from the government. In conclusion, effective policies for job security are needed for both unmarried women and married women without children. For married women with children, there is a need for strengthening on-going housing policies.

Stevia rebaudiana의 항산화 효과

정은혜(Eun Hye Jung),서혜림(Hye Lim Seo),김민규(Min Gyu Kim),김영우(Young Woo Kim),조일제(Il Je Cho) 한의병리학회 2013 동의생리병리학회지 Vol.27 No.6

5·31 교육개혁이 남녀 대학생 수에 미친 차별적 효과분석

임병인 ( Byung In Lim ),서혜림 ( Hye Rim Seo ) 한국경제통상학회 2021 경제연구 Vol.39 No.3

본 연구는 1980년도부터 2018년도까지 총 39개년의 시계열 자료를 ARDL-UECM 모형에 적용하여 5·31 교육개혁으로 도입된 교육정책(대학설립준칙주의, 수시 전형)과 다른 사회경제적인 변수들이 남녀 대학생 수에 미친 장기관계를 추정하여 그 결과들을 분석하였다. 추정결과, 첫째, 대학 설립준칙주의 시행과 수시 전형제도는 남녀 대학생 수에 모두 양(+)의 영향을 미치나, 여자 대학생에게 더 큰 양(+)의 영향을 끼쳤다. 이로써 5.31 교육개혁으로 인해 도입된 대학설립 준칙주의와 수시 전형제도는 여자 대학생에게 더 큰 혜택으로 돌아갔음을 알 수 있다. 둘째, 가구원 수가 줄어들수록, 실질 가구소득이 증가할수록 남녀 대학생 수가 증가하였지만, 여자 대학생 수에 더 큰 영향을 주었다. 이는 남아 선호 인식 약화와 자녀 수 감소와 소득증가로 성별과 무관하게 자녀에 대한 교육 투자를 증가시켰기 때문이라고 보인다. 이상에서 5.31 교육개혁으로 인해 도입된 두 정책과 사회경제적인 변화가 남녀 대학생의 증가에 기여하였지만, 여자 대학생에게 상대적으로 더 유리하여 여자의 대학진학률이 1990년대 후반부터 급격하게 증가하여 2005년 남자를 추월한 뒤 그 추세를 유지하고 있는 현상을 설명해준다. 또한, 5.31 교육개혁 수혜자 집단으로서의 여자 대학생 증가가 2000년대 이후 여성의 경제활동 참가율 증가에 기여하면서 경제 활동 참가율의 남녀 격차 축소에 일정 정도 기여하고 있다는 정책적인 시사점을 도출할 수 있다. This study applies the 39 years time-series data(1980-2018) to the ARDL-UECM model, and estimates the long-term relationship if two educational policies, i.e., the university establishment rules and the early admission introduced by the 5.31 education reform, have an impact on the number of male and female university students. Empirical findings are as follows: first, the 5.31 education reform policy benefits female university students bigger than male students. Second, the less the number of households, the more the household income, the higher the number of both male and female university students, also meaning that these have greater impact on the number of female university students than male. It results from weakening the perception of preference for boys, a decrease in the number of children, and an increase in income. In these contexts, it can be said that both two policies and socio-economic changes are also beneficial to female university students, and further, the reason why women's university enrollment rate has increased rapidly since the late 1990s, overtaking male students’ rate in 2005 and then maintained those trends. In addition, we derive another following policy implication: more female students have entered the labor market since the 5.31 education reform than before, and thus led to the participation rate of women in economic activity higher, and then contributed to narrowing the gap between male and female’s participation rate.

夏枯草의 간세포에서 항산화 및 미토콘드리아 보호효과

장미희(Mihee Jang),서혜림(Hye Lim Seo),김상찬(Sang Chan Kim),김영우(Young Woo Kim) 한의병리학회 2016 동의생리병리학회지 Vol.30 No.1

트레드밀 운동과 타우린 섭취가 streptozotocin 유도 당뇨쥐의 체중, 혈당, 인슐린 및 콜레스테롤 농도에 미치는 영향

김세종 ( Kim Se Jong ),서혜림 ( Seo Hye Lim ),이현미 ( Lee Hyeon Mi ),염종우 ( Yeom Jong U ),김경환 ( Kim Gyeong Hwan ),장은숙 ( Jang Eun Sug ),백영호 ( Baeg Yeong Ho ),전병학 ( Jeon Byeong Hag ) 한국운동영양학회 2003 Physical Activity and Nutrition (Phys Act Nutr) Vol.7 No.3

Exercise and taurine has been known to be benficial to the diabetic patients. But it combinatory effect has not been studied. This study aims to understand whether taurine intake has a repressive effect on blood levels of glucose, insulin and lipids of the streptozotocin-induced hyperglycemic diabetic rat, and also whether combinational treatment of taurine intake and low-intensity exercise has a synergistic repressive action. Taurine was freely supplied as 1 % solution. The blood glucose level was not suppressed in the taurine-intake diabetic rat, but the blood levels of insulin and lipid such as total colesterol, HDL-cholesterol, LDL-cholesterol and triglyceride was reverted. Exercise (25 mill/day, 5 time/week,15 m/min) alone has a repressive effect on the hyperglycemic index. The combinatory effect of taurine and exercise before and after streptozotocin injection was examined. When taurine was supplemented with exercise before streptozotocin injection, the blood glucose level was surprisingly unchanged. but the blood levels of insulin and lipids were mildly recovered. When taurine was supplemented with exercise after streptozotocin injection, the blood glucose level was also unchanged, but the blood levels of insulin and lipids were mostly recovered. These results suggest that taurine itself has no beneficial effect on the blood glucose level and no combinatory effect with exercise, although it has a positive effect on the blood insulin levels and also serum lipids.

Sonovue 초음파 조영제를 이용한 장비 간 Mechanical Index의 변화에 따른 신호 강도 비교연구

이재경(Jae-kyung Lee),서혜림(Hye-lim Seo),김상현(Sang-Hyun Kim),김명석(Myung-seok Kim) 대한초음파의료영상학회 2019 대한초음파의료영상학회 학술대회 초록집 Vol.4 No.1

곽향의 간세포 보호효과

장미희 ( Mi Hee Jang ),변성희 ( Sung Hui Byun ),서혜림 ( Hye Lim Seo ) 대구한의대학교 제한동의학술원 2015 東西醫學 Vol.40 No.1,2

Abbreciations: TVE, Teucrjum veronjcOldes water extract: MTT, 3- (4,5-dimethylthiazoJ) -2,5-diphenyltetrazolium bromide: FACS, fluorescence activated cell sorter: Rh123, Rhodamine 123 Objectives: Teucrium veronicoides is Korean traditional herb that has effect on anti-cancer and anti-inflammation. We examined the hepatocyte protective effect of Teucrium veronicoides water extract (TVE) on apoptosis, mitochondrial dysfunction, ROS production and AMPK activation. Methods: To evalute the hepatocyte protective effect of TYE, we examined the cell viabillity, inhibition of mitochondrial dysfunction and AMPK activation using MTT assay, western blotting and flourescnece activated cell sorter (FACS) on AA+iron induced HepG2 cells known as the human-drived hepatocyte cell. We also examined ROS production on AA+iron induced HepG2 cells. Results: TVE inhibited the cell death and decrease in the apoptosis-associated protein levels such as pro-caspase, PARP on AA+iron induced HepG2 damage. TVE also protected HepG2 cell against AA+iron induced mitochondrial membrane dysfunction. Conclusion: TVE protected hepatocytes against AA+iron induced-apoptosis, MMP dysfunction and ROS production. And TVE induced AMPK activation.

Plyometric Training과 비타민 C 투여가 남고생의 체력, 혈중 젖산, 암모니아 및 젖산탈수소효소에 미치는 영향

장준원,김세종,이현미,서혜림,조현숙,고정림,염종우,예정복,백영호 釜山大學校 附設 體育科學硏究所 2003 體育科學硏究所 論文集 Vol.19 No.-

The purpose of this was to examine the effect of plyometric training and vitamin C ingestion on physical fitness and fatigue substance....