Anticancer Effects of the Engineered Stem Cells Transduced with Therapeutic Genes via a Selective Tumor Tropism Caused by Vascular Endothelial Growth Factor Toward HeLa Cervical Cancer Cells

김혜선,최경철,이보림,황경아,김승우 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

The aim of the present study was to investigate the therapeutic efficacy of genetically engineered stem cells (GESTECs) expressing bacterial cytosine deaminase (CD) and/ or human interferon-beta (IFN-) gene against HeLa cervical cancer and the migration factors of the GESTECs toward the cancer cells. Anticancer effect of GESTECs was examined in a co-culture with HeLa cells using MTT assay to measure cell viability. A transwell migration assay was performed so as to assess the migration capability of the stem cells to cervical cancer cells. Next, several che-moattractant ligands and their receptors related to a selective migration of the stem cells toward HeLa cells were determined by real-time PCR. The cell viability of HeLa cells was decreased in response to 5-fluorocytosine (5-FC), a prodrug, indicating that 5-fluorouracil (5-FU), a toxic metabolite, was converted from 5-FC by CD gene and it caused the cell death in a co-culture system. When IFN-β was additionally expressed with CD gene by these GESTECs, the anticancer activity was significantly increased. In the migration assay, the GESTECs selectively migrated to HeLa cervical cancer cells. As results of real-time PCR, chemoattractant ligands such as MCP-1, SCF, and VEGF were expressed in HeLa cells, and several receptors such as uPAR, VEGFR2, and c-kit were produced by the GES-TECs. These GESTECs transduced with CD gene and IFN- may provide a potential of a novel gene therapy for anti-cervical cancer treatments via their selective tumor tropism derived from VEGF and VEGFR2 expressions between HeLa cells and the GESTECs.

급성심근경색증에서의 심장자기공명영상술의 진단 정확도와 심근 생존력 평가: TI-201 심근관류 SPECT와의 비교

김혜선,박동우,김용수,김영선,최요원,전석철,서흥석,함창곡,김순길,안유헌,최윤영,박충기,Kim Hye-seon,Park Dong Woo,Kim Yongsoo,Kim Young-sun,Choi Yo Won,Jeon Seok Chul,Seo Heung Suk,Hahm Chang Kok,Kim Soon Kil,Ahn You hern,Choi Yoon Young,P 대한자기공명의과학회 2003 Investigative Magnetic Resonance Imaging Vol.7 No.2

목적 : 급성심근경색을 진단하는데 있어 심장자기공명영상의 유용성을 알아보고, 생존심근을 평가하는데 있어 Tl-201 심근관류 SPECT와 비교하고자 하였다. 대상 및 방법 : 흉통 및 호흡곤란이 있는 환자 중 심장자기공명영상과 Tl-201 SPECT를 시행한 17명을 후향적으로 조사하였다. 심장자기공명영상에서는 T2강조영상에서 심벽의 고신호 강도 유무, 영화영상에서 심벽 운동, Gd-DTPA 조영 증강 후 좌심실 벽의 관류 결손 및 주입 15분 후의 지연조영증강 유무를 평가하였다. T1-201 SPECT에서는 아데노신 부하 영상과 휴식기 영상을 얻어 관류 결손 유무와 관류 결손시 가역성 여부를 평가하였다. 결과 심장자기공명영상과 T1-201 SPECT 모두 급성심근경색을 진단하는데 통계적으로 의미가 있었고, T2강조영상, T1-201 SPECT, 지연조영증강 및 심벽운동 순으로 연관성이 있는 것으로 나타났고, 관류 결손은 통계적 유의성이 없었다. 생존심근 평가에 있어서는 두 검사간 유의한 차이가 없었으며, T2강조영상과 관류영상에서 T1-201 SPECT와 높은 일치율, 지연조영증강 및 심벽운동은 낮은 일치율을 보였다. 결론 : 심장자기공명영상은 급성심근경색을 진단하는데 매우 유의한 진단율을 보였고, 생존 심근여부의 판단에 있어 SPECT와 높은 일치율을 보였다. 그러나 각 검사별 판독 기준과 방법에서 많은 연구가 필요할 것으로 생각된다. Purpose : To assess the usefulness of cardiac MR imaging (MRI) in the diagnosis of acute myocardial infarction and in the assessment of myocardial viability in comparision with T1-201 SPECT. Materials and Methods : We retrospectively studied 17 patients who complained of chest pain and dyspnea with cardiac MRI . The patients were evaluated for the presence or absence of high signal intensity on T2-weighted image (T2wI), abnormal wall motion on 2D-FIESTA, perfusion defect on Gd-DTPA enhanced T1WI, and delayed myocardial enhancement on 15-minutes delay Gd-DTPA enhanced T1WI. The results were correlated with the images on T1-201 SPECT, taken at rest and stress, through which reversibility of perfusion defect was assessed. Results : Both cardiac MRI and T1-201 SPECT proved to be useful methods for diagnosing acute myocardial infarction. In order of decreasing correspondence, T2WI, T1-201 SPECT, delayed enhancement study, and wall motion images all showed significant statistical correlation with the clinical diagnosis of myocardial infarction. Perfusion MRI, on the other hand, showed no significant statistical difference was found between T1-201 SPECT and cardiac MRI. The results on T2WI showed high accordance with those on Tl-201 SPECT, while delayed myocardial enhancement and wall motion studies showed no agreement with Tl-201 SPECT. Conclusion : Cardiac MRI is useful method for diagnosis of acute myocardiac infarction. With respect to the assessment of myocardial viability, the results obtained on cardiac MRI showed high agreement with those on Tl-201 SPECT. However, further study is necessary at this point for standardization and establishment of the methods for assessing myocardial viability on cardiac MRI.

국내 영업을 하지 않는 외국 회사의 부정경쟁행위 및 응용미술저작물 관련 저작권 침해 주장 : 서울중앙지방법원2010. 7. 21. 선고2009가합138388 판결

김혜선 서울대학교 기술과법센터 2011 Law & technology Vol.7 No.3

미혼남녀의 결혼준비도

김혜선,이정읍 대한가정학회 2003 Human Ecology Research(HER) Vol.41 No.2

The purpose of this study was to investigate premarital preparation of single men and women. The number of subjects was 393 premarital couples(196 males and 197 females) in Seoul, Incheon, Daejeon, Gwangju, Pusan and Daegu. The self-administered questionnaire was composed of premarital preparation evaluation scale developed by Kim Hye Seon & Shin Soo Ah(2002). The data was analyzed by frequencies, percentages, mean Cronbach'sα, t-test, ANOVA, Duncan-test. The major findings were as follows: First, the level of permarital preparation status was somewhat higher than average and the level of premaital preparation awareness was very high. Second, preparation status of premarital couples was shown significant difference by some bariables including sex, age, occupation, the period dating, religion. Third, preparation awareness of premarital couples was shown a significant difference by some variables including sex, age, academic ability, the period of dating.

발달장애 유아의 자세조절과 원시반사조정 그리고 양측운동조절을 위한 전정자극 훈련의 효과

김혜선,송병호 檀國大學校 特殊敎育硏究所 2007 特殊敎育要求 兒童硏究 Vol.18 No.-

이 연구는 세분화된 전정자극 훈련이 중도 발달지체 유아의 자세조절력과 원시반사 통합 그리고 양측운동 통합의 전정기능과 전반적 발달에 효과적인지 알아보는데 그 목적이 있다. 연구대상은 어린이집에서 통합교육을 받고 있는 평균 5세의 중도 발달지체 유아 세명이다. 측정도구는 Donald A. Berk과 Georgia A. DeGangi가 편찬한 드간지 벅 감각통합검사(DeGangi - Berk Test of Sensory Integration)에서 설명한 36개의 자세조절과 원시반사 통합 그리고 양측 운동통합의 하부항목들 중 유아들에게 적합한 10개를 선택하여 약식의 측정도구를 제작하여 적용하였고 전반적 발달정도를 비교하기 위하여 덴버 II(DENVER II development screening test; William, 1967)를 사용하였다. 훈련내용은 수직, 수평 그리고 회전으로 이루어진 세 가지 전정자극 훈련을 실험기간 동안에 주 2회 실시하고 사전 사후 검사를 통해 그 효과를 비교하였다. 이 연구의 목적에 따른 결과는 다음과 같다. 첫째, 전정훈련후의 점수가 훈련 전 보다 현저히 높아졌으므로, 세분화된 전정자극 훈련은 중도 발달지체 유아의 자세조절력을 향상시키는데 효과적인 것으로 나타났다. 둘째, 세분화된 전정자극 훈련은 중도 발달지체 유아의 원시반사 통합을 향상시키는데 효과적이다. 유아 1과 2는 훈련 후에 만점에 도달할 수 있었고, 유아 3은 처음에 안정적인 통합을 보이면서도 전정자극훈련의 영향에 변동이 없었다. 셋째, 점수와 관찰내용으로 보았을 때, 세분화된 전정자극 훈련은 중도 발달지체 유아의 양측 운동 통합을 향상시키는데 효과적인 것으로 나타났다. 연필과 종이의 사용방법이 달라지고 오류를 수정하는 등 평가에 임하는 태도에 변화를 보인 것이다. 넷째, 세분화된 전정자극 훈련은 중도 발달지체 유아의 전반적 발달을 향상시키는데 효과적인 것으로 나타났다. 유아1과 3이 높은 점수 차를 얻었고, 차이를 보이지 않은 유아2도 과제를 수행하는 동안 전반적인 움직임의 안정성 유지에 발전을 보였다. 이와 같은 연구결과로 운동감각이 현저히 증가되는 시기인 유아기의 세분화되고 간단하지만 정확한 전정자극 훈련이 중도 발달지체 유아의 자세조절력과 원시반사 통합 그리고 양측운동 통합을 비롯하여 일상생활 적응력, 대·소근육 그리고 언어발달의 전반적 발달영역에 모두 효과적인 치료방법임을 알게 되었다. The purpose of this research was to find out the effects of specific vestibular stimulation on the abilities of postural control and primitive reflex integration with bilateral movement integration. The subjects were three children with developmental disability average age 5 year-old. The measurement tools were 10 questionary from the DeGangi Berk Tes of Sensory Integration and Denver II-Development Screening test. Three ways of vestibular stimulation training include vertical and horizontal rotation were performed two times a week. The measurement were taken pre and post training program. The results were as followings; First the specialized vestibular stimulation training was effective on controling the posture for the children with developmental. Second, the specialized vestibular stimulation training was effective for the development of primitive reflex integration. Child 1 and 2 reached to highest point. Chid 3 showed steady integration at the beginning, but he showed any change with the vestibular stimulation. Third, the results of tests showed specific vestibular stimulation was effective on bilateral movement integration. Child was different with holding the pencil and using the paper. Fourth, the specific vestibular stimulation was effective on general development of child with developmental delay. Child 1 and 3 showed significant difference, and child 2 showed a steady stability even though it was not significant. Therefore, from the results the vestibular stimulation training was effective on development of postural control and general developmental for the children with developmental delay

도시부도로 지점교통정보를 이용한 통행시간 산정방법 비교분석

김혜선,김보성,김성호 한국ITS학회 2014 한국ITS학회 학술대회 Vol.2014 No.05

통화수요와 유동성함정의 추정: 한국과 일본의 비교연구

김혜선 한국제도∙경제학회 2017 제도와 경제 Vol.11 No.3

본 연구에서는 통화수요 관계(money demand relation)와 유동성함정(the liquidity trap)의 이론적 존재를 한국과 일본의 통화 및 거시변수 자료를 이용하여 분석하였다. 이를 위하여, 고전적 통화수요 모형에 최근의 추정방식을 적용하여 수정한다. 즉, ‘베이지언 박스-콕스 모형’(Bayesian Box-Cox transformation)을 이용하여 통화수요 관계식을 확장하고 ‘마코프 체인 몬테카를로(Markov Chain Monte Carlo, MCMC) 방식’을 적용하여 실증 분석한다. 그 결과, 한국과 일본의 경우 모두에서 유동성 함정을 나타내는 이자율의 이론적 하한선이 0과 1 사이에서 존재하는 것으로 나타났으며, 한국모형은 0.543, 일본모형은 0.000499로 추정되었다. 또한, 통화수요모형의 형태에 관련된 파라미터가 한국모형에서는 0.842로 양(+)의 값으로 추정되었으나 일본모형에서는 –0.037로 음(-)의 값으로 나타났다. 즉, 통화수요이론에서 제시하는 표준모형이 한국과 일본에 상이하게 적용될 수 있음을 확인했다. In this paper, we modify the money demand model to show the existence of liquidity trap. We incorporate the liquidity trap into the Bayesian Box-Cox transformation of the money demand relation, and improve the empirical procedure using Monte Carlo Markov Chain(MCMC). The estimation is based on Korean and Japanese data. First, the model is estimated to have the lowest bound interest rate between 0 and 1. It is estimated to be 0.543 and 0.000499 for Korea and Japan, respectively. Second, we found that the parameter for measuring the degree of log-linearization in Japanese model has a significantly negative sign.

교사되기를 향한 유치원 초임교사들의 교육 이야기

김혜선 한국유아교육학회 2006 유아교육연구 Vol.26 No.2

한국 근해의 난바다곤쟁이 Euphausia pacifica의 지방 조성에 의한 섭식 생태 비교

김혜선,주세종,고아라 한국해양과학기술원 2010 Ocean and Polar Research Vol.32 No.2

Dietary lipid biomarkers (fatty acids, fatty alcohols, and sterols) in adult specimens were analyzed to compare and understand the feeding ecology of the euphausiid, Euphausia pacifica, from three geographically and environmentally diverse Korean waters (Yellow Sea, East China Sea, and East Sea). Total lipid content of E. pacifica from Korean waters was about 10% dry weight (DW) with a dominance of phospholipids (>46.9% of total lipid content), which are known as membrane components. A saturated fatty acid, C16:0, a monounsaturated fatty acid, C18:1(n-9), and two polyunsaturated fatty acids, C20:5(n-3) and 22:6(n-3), were most abundant (>60% of total fatty acids) in the fatty acid composition. Some of the fatty acids showed slight differences among regions although no significant compositional changes of fatty acids were detected between these regions. Phytol, originating from the side chain of chlorophyll and indicative of active feeding on phytoplankton, was detected all samples. Trace amounts of various fatty alcohols were also detected in E. pacifica. Specifically, krill from the Yellow Sea showed relatively high amounts of longchain monounsaturated fatty alcohols (i.e. 20:1 and 22:1), generally found in herbivorous copepods. Three different kinds of sterols were detected in E. pacifica. The most dominant of these sterols was cholest-5-en- 3β-ol (cholesterol). The lipid compositions and ratios of fatty acid trophic markers are indicative of herbivory in E. pacifica from the Yellow Sea and East Sea (mainly feeding on dinoflagellates and diatoms, respectively). The lipid compositions and ratios of fatty acid trophic markers are indicative of carnivory or omnivory in E. pacifica from the East China Sea, mainly feeding on microzooplankton such as protozoa. In conclusion, lipid biomarkers provide useful information about krill feeding type. However, further analyses and experiments (i.e. gut content analysis, in situ grazing experiment, etc.) are needed to better understand the feeding ecology of E. pacifica in various marine environments.

절임 기간에 따른 야콘 간장 장아찌의 품질 특성

김혜선,조인희,김경미,김영,김기창 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10