“호모 루덴스”로 본 조선족 민요 연구

김춘매 천진사범대학교 한국문화연구중심 2022 중한언어문화연구 Vol.23 No.-

荷兰学者约翰·赫伊津哈在其著作《Homo Ludens》中正式提出了“游戏人”。人们常常认为游戏是文化中创造出来的, 但赫伊津哈认为游戏比文化更先进, 试图将游戏理解为一种文化现象而不是一种生理现象。他觉得所有的社会活动都是游戏, 所以民谣自然亦是一种游戏。本文通过赫伊津哈的游戏理论进一步探讨朝鲜族民谣的特征和内涵。从赫伊津哈的“游戏人”视觉切入, 解读朝鲜族民谣对生活的乐观性和未来指向性。

Liriodenine이 PC12 세포중의 Dopamine 생합성에 미치는 영향

김춘매,이재준,윤수옥,김유미,김영균,유시용,이명구 한국생약학회 2003 생약학회지 Vol.34 No.1

라지오방송의 언어에 대하여

김춘매(Kim Chunmae) 길림성민족사무위원회 2014 중국조선어문 Vol.195 No.-

종-특이적 PCR 분석법을 이용한 신속하고 간편한 말전복의 종판별법 개발

동춘매,이미난,강정하,박중연,남보혜,노재구,김필연,조영아,김은미 한국패류학회 2018 The Korean Journal of Malacology Vol.34 No.1

This study was performed to identify rapidly Haliotis gigantea using polymerase chain reaction with species-specific primers. Around 680 bp of the mitochondrial ND5 gene region from four Haliotis species were aligned and species-specific forward primer was designed based on the single nucleotide polymorphism (SNP) from H. gigantea. The optimal PCR conditions were selected by cross reactivity using gradient PCR method from 55°C to 66°C. Species-specific PCR (SS-PCR) amplification reactions with two pairs of primers were performed for a five specimens of Haliotis species. SS-PCR leads to a species specific amplification of a 1,006 bp fragment in H. discus hannai, H. discus discus, H. madaka and 786 bp in H. gigantea, respectively. The two different sizes of each PCR products can be quickly and easily detected by single gel electrophoresis. The sensitivity of the SS-PCR was up to 1ng/μl DNA as a starting concentration in H. gigantea tested. Therefore SS-PCR technique with species-specific primer based on SNP could be a powerful tool for discrimination of H. gigantean and can contribute to the prevention of falsified labeling of this species.

Microsatellite marker 분석을 이용한 명태(Theragra chalcogramma) 5 집단의 유전적 다양성 및 유연관계 분석

동춘매(Chun Mae Dong),강정하(Jung-Ha Kang),변순규(Soon-Gyu Byun),박기영(Kie-Young Park),박중연(Jung Youn Park),공희정(Hee Jeong Kong),안철민(Cheul Min An),김군도(Gun-Do Kim),김은미(Eun-Mi Kim) 한국생명과학회 2016 생명과학회지 Vol.26 No.11

한류성 어종인 명태는 우리나라 동해를 비롯한 일본, 러시아 북부의 오호츠크해, 베링해, 알래스카 등지에 서식하는 중요한 수산자원으로, 우리나라에서는 그 어획량이 매년 감소하고 있어, 그 자원량의 회복과 보존 및 관리가 필요한 대표적 어종이다. 그러나, 이러한 중요성에도 불구하고 국내에서 명태의 유전학적 집단 분석에 관한 연구는 많이 수행되지 않은 실정이다. 본 연구에서는 우리나라 동해, 러시아, 미국 명태 집단 및 일본 명태 집단과의 유전적 다양성과 유연관계를 분석하여 명태자원의 보존과 관리를 위한 과학적 자료를 제공하기 위해 유전적 다양성 및 계군 분석에 널리 사용되고 있는 microsatellite marker (msDNA) 8개를 사용하여 명태 집단의 유전자형을 분석하였다. 우리나라 동해, 러시아, 미국 및 일본 집단에서 채집된 총 186개체를 분석한 결과, 대립유전자수는 최소 7.13개에서 최대 10.63개로 나타났고, 평균 대립유전자의 수는 9.05개로 나타났다. 기대치와 관찰치 이형 접합율은 각각 0.698과 0.732로 조사되어, 현재 확보된 명태 집단의 유전적 다양성은 비교적 잘 유지되고 있는 것으로 나타났다. 유전학적 유연관계 분석을 위한 유전적 거리, Pairwise FST값, UPGMA와 주성분분석, AMOVA test 분석 결과, 우리나라 동해, 러시아, 미국의 명태 집단 간 유전적 차이는 거의 없었으나 일본 명태 집단과는 낮은 수치이지만 유의한 유전적 차이가 있음을 확인하였다(p<0.05). 본 연구에서 확인된 유전학적 분석을 통한 명태집단의 유전적 특성 및 주변국 집단과의 유연관계 분석결과는 우리나라 동해의 중요한 수산유전자원으로서의 명태에 대한 중요한 과학적인 근거자료가 될 것이며, 앞으로 명태 자원의 보존, 평가 및 이용에 활용 가능한 정보를 제공할 것이다. A comprehensive analysis of the genetic diversity and relationship of the cold-water fishery walleye pollock (Theragra chalcogramma), the most abundant economically important fishery resource in the East sea of Korea, has not been carried out, despite its importance in Korea. The present study assessed the genetic diversity and relationship between five walleye pollock populations (Korean population, Russian population, USA population, and Japanese populations) of T. chalcogramma using eight microsatellite DNA (msDNA) markers to provide the scientific data for the preservation and management of the Pollock fishery resource. The results of the analysis of 186 individuals of the Pollock revealed a range of 7.13–10.63 numbers of alleles (mean number of alleles=9.05). The means of observed heterozygosity (HO), expected heterozygosity (HE) were 0.732 and 0.698, respectively. The results of genetic distance, Pairwise FST, UPGMA (UPGMA: un-weighted pair-group method with an arithmetical average) (the phylogenetic tree), PCA (PCA: Principal Coordinate analysis) analysis pointed to significant differences between the Korean population, Russian population, USA population, and Japanese populations, although small (p<0.05). These results shed light on the genetic diversity and relationships of T. chalcogramma and can be utilized for research on the evaluation and conservation of Korean T. chalcogramma as genetic resources.

중국 여가 산업의 발전상황과 특징에 관한 연구

하춘매,김명화 한국여가레크리에이션학회 2006 한국여가레크리에이션학회지 Vol.30 No.1

◈This thesis studied about the expansive trend of the leisure industry in china which matured by economic growth and the change of the industrial structure and the meaning of the leisure industry through the strategy of country in China.The growth and rearing of the leisure industry in China is noticed by a promising industry which contribute the stimulation of the domestic demand and well balanced development among regions. In addition, it is contributed the Chinese’s improvement in the quality of life that the reduction of business hours like Western Europe and great holidays such as CHUNJIL, LAODONGJIL(labor day), GUOQINGJIL.Through the journey of the weekend, people tend to recover their body and soul. These tendencies made a new word which is called “A holiday economy”.According to those reasons, recent Chinese people spend their time in trip, theater and music hall. The new policy of the work hours like Western Europe and great holidays gave 115 regular holidays of the year to Chinese and the satisfaction of the steady economic growth during 25 years make people become to realize the importance of their daily life as a consumer.The growth of the leisure industry in china has steady economic growth which chases the improvement in quality of the life. Also it has very special meaning by important way which can solve the inequality growth among regions in China. Needless to say, it is true that the latest leisure industry in china has many problems which had to improve because the history of the leisure industry is so short compare to the advanced countries.Nevertheless, leisure industry is not only one of the promising industriesbut also expected very good mean which can build harmonious socialism. Ⅳ. 중국여가 산업의 문제점

차세대 염기서열 분석법을 이용한 방어(Seriola quinqueradiata)의 microsatellite 마커의 개발 및 유전적 특성 분석

동춘매(Chun Mae Dong),이미난(Mi-Nan Lee),김은미(Eun-Mi Kim),박중연(Jung Youn Park),김군도(Gun-Do Kim),노재구(Jae Koo Noh) 한국생명과학회 2020 생명과학회지 Vol.30 No.3

본 연구는 차세대 염기서열 분석법(NGS)을 이용하여 방어의 microsatellite 마커를 개발하고, 개발된 마커를 이용하여 방어 집단의 유전적 특성을 분석하기 위해 수행되었다. 차세대 염기서열 분석 장비인 Illumina Hiseq2500를 이용하여 총 28,873,374개의 read들을 얻어 assembly를 수행한 결과, 전체의 약 1.6%에 해당하는 466,359개의 read들이 assembly 되었으며, 이 read들의 총 길이는 7,247,216,874 bp로 확인되었다. 크기가 518 bp 이상이 되는 contig는 30.729개로 나타났으며, 이 중 microsatellite 영역을 포함하는 contig 132개(0.43%)를 1차로 선별하고, PCR 증폭 여부 및 유전자형 분석을 통해 microsatellite 후보 60개를 2차로 선별하였다. 그 중 방어집단의 마커로서 유용한 15개의 microsatellite 마커를 선택하였다. 방어집단을 대상으로 개발된 15개의 microsatellite 마커로 분석한 결과, 관찰된 유효 대립유전자수(NA)는 평균 18.5(11~30)로 나타났다. 평균 관측치 이형접합도(HO)와 평균기대치 이형접합도(HE)는 각각 0.812(0.431~0.972)와 0.896(0.782~0.949)으로 나타났다. 다형성이 관찰된 모든 microsatellite 마커 간의 연관불평형은 나타나지 않았으며, 해산어의 평균 HE 값인 0.79 이상의 수치를 나타내었다. 따라서 본 연구에서 개발된 15개의 microsatellite 마커는 방어 집단의 유전적 다양성 분석에 유용할 것으로 사료된다. This study was conducted to develop microsatellite markers in Seriola quinqueradiata using next-generation sequencing. A total of 28,873,374 reads were generated on an Illumina Hiseq2500 system, yielding 7,247,216,874 bp sequences. The de novo assembly resulted in 466,359 contigs. A total of 132 contigs (0.43%), including 60 microsatellite loci, were derived from 30,729 contigs longer than 518 bp. A total of 60 primer sets were designed from the 132 microsatellite loci. A total of 15 polymorphic nuclear microsatellite loci were chosen to evaluate population genetic parameters in the parents and offspring. The mean number of effective alleles was 18.5, ranging from 11 to 30. The observed heterozygosity (HO) and expected heterozygosity (HE) ranged between 0.431 and 0.972 with an average of 0.812 and from 0.782 to 0.949 with an average of 0.896, respectively. No significant linkage disequilibrium was observed after Bonferroni revision in any loci. The results show that the 15 polymorphic nuclear microsatellite markers can be used to study the population and conservation genetics of S. quinqueradiata in Korea. To ensure the success of artificial seedling production technology, genetic variations between the parent and offspring populations should be monitored, and inbreeding should be controlled.

유전자변형작물의 개발 현황 및 안전관리 동향

동춘매,김주원,강정하,박중연,김군도,공희정 한국수산해양교육학회 2017 水産海洋敎育硏究 Vol.29 No.6

Genetically modified organisms (GMOs) are created by biotechnology to improve crop production. The commercialization and commercial cultivation of GMOs are gradually increasing. The area planted with genetically modified crops, which was only 1.7 million hectares in 1996, increased by 18.51 million hectares in 2016. Compared to 1996, it has increased by more than 100 times. The country that grows the most genetically modified crops is the United States; the area of cultivation in the United States is 72.9 million hectares. The cultivation area of genetically modified crops is increasing annually in the world, in the order of Brazil (49.1 million hectares), Argentina (2.380 million hectares), Canada (1.160 million hectares), and India (10.80 million hectares). Korea has not yet grown genetically modified crops, but it has been importing genetically modified crops for food and feed since 2001. In 2016, 22.4 million tons (21%) were imported for food and 77.4 million tons (79%) were imported for feed. In addition, many genetically modified crops, including rice, pepper, soybean, and grass, have been developed by research institutes, universities, and companies, and some of these crops are expected to be put into practical use soon in Korea at the safety evaluation stage. The number of genetically modified crops has increased steadily worldwide to benefit societies, but these crops may pose risks to the ecological environment and to human health and therefore require careful regulation. This study investigated the current status of genetically modified crops, both domestically and overseas, and examined the safety management trends and labeling systems for GMOs.

단체급식소에서 제공되는 음식의 적정분량 설정에 관한 연구(ㅣ) : 국민학교급식을 중심으로 in elementary school food service

金蕙英,金春妹 성신여자대학교 생활문화연구소 1994 生活文化硏究 Vol.8 No.-

단체급식소에서 제공되는 음식의 적정분량 설정에 관한 연구(2) : 산업체급식소와 대학기숙사 급식소를 대상으로

김혜영,고성희,김춘매 한국식생활문화학회 1997 韓國食生活文化學會誌 Vol.12 No.1

Well-balanced meal is very important in its quantity and quality. Especially on the quantity field it becomes difficult for a foodservice operation to decide proper portion for individuals uniformly. These study was focused to setting up a proper portion by each food service operation. The results obtained were: 1. Individual consumption size from dormitory food service of college: cooked rice 282 g, soups 161 g, pot stewes 162 g, stir fries 53 g, stews 32 g, kimchies 47 g, fresh and boiled salads 43 g, one course dishies 477 g, pan broiles 44 g, meunieres 124 g. Individual consumption size from industry foodservice (white collar worker): cooked rices 228 g, soups 205 g, pot stewes 251 g, stir fries 20 g, stewes 76 g, kimchies 57 g, fresh and boiled salads 36 g, one course dishies 423 g, pan broiles 63 g, meunieres 38 g. 2. Proper portion of meal based on a statistical data is as follows: at college foodservice - cooked rices 280∼290 g, soups 155∼170 g, pot stewes 170 g, stir fries 60 g, stewes 35 g, kimchies 40∼60 g, fresh and boiled salads 50 g, one course dishies 480 g, pan broiles 50 g, meunieres 130 g and at industry foodservice (white collar worker) - cooked rices 220∼250 g, soups 210 g, pot stewes 250 g, stir fries 20 g, stewes 80 g, kimchies 60 g, fresh and boiled salads 40 g, one course dishies 430 g, pan broiles 70 g, meunieres 40 g.